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The map label for this gene is mlaA [H]

Identifier: 77460071

GI number: 77460071

Start: 4352722

End: 4353411

Strand: Reverse

Name: mlaA [H]

Synonym: Pfl01_3850

Alternate gene names: 77460071

Gene position: 4353411-4352722 (Counterclockwise)

Preceding gene: 77460074

Following gene: 77460070

Centisome position: 67.62

GC content: 59.42

Gene sequence:

>690_bases
ATGCGCTGGAGCCATCCTCTTGCTCAGCTTTGTGTATGTGCCAGCGTGATGCTGGTGCCGTTCGCCGCTCAAGCCGCAAC
GGAAGAAGACCCTTGGGAAAGCGTCAACCGTCCGATCTTCCAGTTCAACGATTTCGTCGACACCTATGCGCTGAAGCCGC
TGGCGCAGGGCTACGAATTTGTTACTCCGCAATTCCTGGAAGACGGCATCCACAACATGTTCCGCAACGTCGGTGATGTC
ACCAACCTGGCGAACAACATTCTTCAGGCCAAACCGGCTGCGGCTGGCGTCGACACCGCGCGCCTGATCTTCAACACCAC
CTTCGGCCTGCTCGGATTCTTCGACGTCGGCACCAAAATGGGCCTGAACCGCAGCGACGAAGACTTCGGCCAGACCCTCG
GCTACTGGGGCGTCGGCAGCGGTCCTTACGTGATGCTGCCACTGATGGGCCCGAGTACCCTGCGCGACGCGCCGTCCAAG
TACGTCGACAGCTTCACCGGCCCGTACCGCTACATCAACGATGTGCCGGTGCGTAACTCGATTTTCGGCCTGAACATCGT
TGATACCCGCGCCAGCCTGCTGTCGAGCGAGAAGCTGATCAGCGGCGACAAGTACACCTTCATCCGCAACGCCTACCTGC
AGAACCGCGAGTTCAAGGTCAAGGACGGGCAGGTCGAAGACGATTTCTGA

Upstream 100 bases:

>100_bases
TTTCACCGGGGGAACTGCCTAAAGTGCCGTTTCCGTCTTATACTGACGCACTAATCGCCGCGGCCCAGGTGCTGCGTCCG
TACTATCCAAGGAGTTTTCT

Downstream 100 bases:

>100_bases
TCTCGGTTGATCGGTATGAAAGGGCGGCCGCGAGGTCGCCTTTTTTGTGGGCGCGTCCAATGATTGGGTTACGGTTGAAG
CCGTTCTTATAACTGCGAGT

Product: VacJ-like lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MRWSHPLAQLCVCASVMLVPFAAQAATEEDPWESVNRPIFQFNDFVDTYALKPLAQGYEFVTPQFLEDGIHNMFRNVGDV
TNLANNILQAKPAAAGVDTARLIFNTTFGLLGFFDVGTKMGLNRSDEDFGQTLGYWGVGSGPYVMLPLMGPSTLRDAPSK
YVDSFTGPYRYINDVPVRNSIFGLNIVDTRASLLSSEKLISGDKYTFIRNAYLQNREFKVKDGQVEDDF

Sequences:

>Translated_229_residues
MRWSHPLAQLCVCASVMLVPFAAQAATEEDPWESVNRPIFQFNDFVDTYALKPLAQGYEFVTPQFLEDGIHNMFRNVGDV
TNLANNILQAKPAAAGVDTARLIFNTTFGLLGFFDVGTKMGLNRSDEDFGQTLGYWGVGSGPYVMLPLMGPSTLRDAPSK
YVDSFTGPYRYINDVPVRNSIFGLNIVDTRASLLSSEKLISGDKYTFIRNAYLQNREFKVKDGQVEDDF
>Mature_229_residues
MRWSHPLAQLCVCASVMLVPFAAQAATEEDPWESVNRPIFQFNDFVDTYALKPLAQGYEFVTPQFLEDGIHNMFRNVGDV
TNLANNILQAKPAAAGVDTARLIFNTTFGLLGFFDVGTKMGLNRSDEDFGQTLGYWGVGSGPYVMLPLMGPSTLRDAPSK
YVDSFTGPYRYINDVPVRNSIFGLNIVDTRASLLSSEKLISGDKYTFIRNAYLQNREFKVKDGQVEDDF

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=225, Percent_Identity=32.4444444444444, Blast_Score=110, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 25551; Mature: 25551

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWSHPLAQLCVCASVMLVPFAAQAATEEDPWESVNRPIFQFNDFVDTYALKPLAQGYEF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
VTPQFLEDGIHNMFRNVGDVTNLANNILQAKPAAAGVDTARLIFNTTFGLLGFFDVGTKM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GLNRSDEDFGQTLGYWGVGSGPYVMLPLMGPSTLRDAPSKYVDSFTGPYRYINDVPVRNS
CCCCCCHHHHHHCCCCCCCCCCEEEEECCCCHHHHHCHHHHHHHCCCCHHHHCCCCCCCC
IFGLNIVDTRASLLSSEKLISGDKYTFIRNAYLQNREFKVKDGQVEDDF
EECCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCCCCCC
>Mature Secondary Structure
MRWSHPLAQLCVCASVMLVPFAAQAATEEDPWESVNRPIFQFNDFVDTYALKPLAQGYEF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
VTPQFLEDGIHNMFRNVGDVTNLANNILQAKPAAAGVDTARLIFNTTFGLLGFFDVGTKM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GLNRSDEDFGQTLGYWGVGSGPYVMLPLMGPSTLRDAPSKYVDSFTGPYRYINDVPVRNS
CCCCCCHHHHHHCCCCCCCCCCEEEEECCCCHHHHHCHHHHHHHCCCCHHHHCCCCCCCC
IFGLNIVDTRASLLSSEKLISGDKYTFIRNAYLQNREFKVKDGQVEDDF
EECCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]