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The map label for this gene is queF [H]

Identifier: 77460074

GI number: 77460074

Start: 4354655

End: 4355485

Strand: Reverse

Name: queF [H]

Synonym: Pfl01_3853

Alternate gene names: 77460074

Gene position: 4355485-4354655 (Counterclockwise)

Preceding gene: 77460075

Following gene: 77460071

Centisome position: 67.65

GC content: 61.85

Gene sequence:

>831_bases
ATGCATCCCGCAGCCGAACATTCGCCGCTGGGCAAATCCAGCGAATACATCGCCACGTACACGCCGTCCCTGCTGTTCCC
GATCCCGCGCACCGCGAAATGGGCCGAATTGGGCCTGACCGCCGAGACCCTGCCGTATAAAGGCGTGGATTTCTGGAACT
GCTTCGAACTGTCGTGGCTGCTGCCGTCGGGTAAACCGGTGGTGGCGATCGGTGAATTCAGCATTCCGGCGGATTCGCCG
AACATCATCGAATCCAAGTCGTTCAAGCTGTACTTGAACTCGCTGAACCAGACGCCGTTCGCCGACACCGCGAGCCTGGA
AGCGACGCTGGTCAAGGATTTGTCCGCTGCTGCCGGCAAACCGGTGGGCGTGCGCGTCCGCAGCCTGAAAGACGTCGAAG
CCGAAGGCGTTGTGGCGCTGCCGGGCGTGTGCATCGACGATCTGGATATCAGCGTCAGTGACTACGAACACCCGCGTCCT
GAGCTGCTGCGCTGCGACGATTCGCGGGTGGTGGAGGAGAGCGTGCACAGCCATCTGCTCAAATCCAACTGCCCGGTGAC
CAGTCAGCCGGACTGGGGCAGCGTGGTGGTGGAATACCGTGGCGCGGCGCTGGATCACGCCAGTTTGCTGGAGTACATCG
TCAGCTTCCGTCAGCACTCGGACTTCCACGAGCAGTGCGTGGAGCGGATCTTCCTCGACCTGCAGCGCCTGCTGAAACCG
GAAAAGCTCACGGTGTTTGCGCGTTACGTGCGCCGGGGCGGGCTGGACATCAACCCGTACCGCAGCACTGAAAGCGTGCA
ACTGCCGAACCACCGTCTGGTTCGTCAGTAA

Upstream 100 bases:

>100_bases
CAAGCGGGAATGGTCGGCAAACTGACTGTCAGTCAGTAAGCACTCAAAGGCGGAAGCAAAGGCTGGTAGAATCCGCTGAT
TCTTCAGTCAGGTTTCCGCC

Downstream 100 bases:

>100_bases
CCCGCCTCGCGGACATGAAAAAGCCCTGCCAGTGATGGCAGGGCTTTTTTGCATTCGCGGGGTCTCAGATGCCCATGCTG
ACCAGGGAATTCATGATGTT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRTAKWAELGLTAETLPYKGVDFWNCFELSWLLPSGKPVVAIGEFSIPADSP
NIIESKSFKLYLNSLNQTPFADTASLEATLVKDLSAAAGKPVGVRVRSLKDVEAEGVVALPGVCIDDLDISVSDYEHPRP
ELLRCDDSRVVEESVHSHLLKSNCPVTSQPDWGSVVVEYRGAALDHASLLEYIVSFRQHSDFHEQCVERIFLDLQRLLKP
EKLTVFARYVRRGGLDINPYRSTESVQLPNHRLVRQ

Sequences:

>Translated_276_residues
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRTAKWAELGLTAETLPYKGVDFWNCFELSWLLPSGKPVVAIGEFSIPADSP
NIIESKSFKLYLNSLNQTPFADTASLEATLVKDLSAAAGKPVGVRVRSLKDVEAEGVVALPGVCIDDLDISVSDYEHPRP
ELLRCDDSRVVEESVHSHLLKSNCPVTSQPDWGSVVVEYRGAALDHASLLEYIVSFRQHSDFHEQCVERIFLDLQRLLKP
EKLTVFARYVRRGGLDINPYRSTESVQLPNHRLVRQ
>Mature_276_residues
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRTAKWAELGLTAETLPYKGVDFWNCFELSWLLPSGKPVVAIGEFSIPADSP
NIIESKSFKLYLNSLNQTPFADTASLEATLVKDLSAAAGKPVGVRVRSLKDVEAEGVVALPGVCIDDLDISVSDYEHPRP
ELLRCDDSRVVEESVHSHLLKSNCPVTSQPDWGSVVVEYRGAALDHASLLEYIVSFRQHSDFHEQCVERIFLDLQRLLKP
EKLTVFARYVRRGGLDINPYRSTESVQLPNHRLVRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=277, Percent_Identity=50.1805054151625, Blast_Score=274, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 30767; Mature: 30767

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRTAKWAELGLTAETLPYKGVDFWNCFELSWL
CCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCHHHHCCCHHCCCCCCCCCCCEEEEEEE
LPSGKPVVAIGEFSIPADSPNIIESKSFKLYLNSLNQTPFADTASLEATLVKDLSAAAGK
CCCCCCEEEEECEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
PVGVRVRSLKDVEAEGVVALPGVCIDDLDISVSDYEHPRPELLRCDDSRVVEESVHSHLL
CCEEEEECCCCCCCCCEEECCCEEECCCCCEECCCCCCCHHHEECCCHHHHHHHHHHHHH
KSNCPVTSQPDWGSVVVEYRGAALDHASLLEYIVSFRQHSDFHEQCVERIFLDLQRLLKP
HCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EKLTVFARYVRRGGLDINPYRSTESVQLPNHRLVRQ
HHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHCCCC
>Mature Secondary Structure
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRTAKWAELGLTAETLPYKGVDFWNCFELSWL
CCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCHHHHCCCHHCCCCCCCCCCCEEEEEEE
LPSGKPVVAIGEFSIPADSPNIIESKSFKLYLNSLNQTPFADTASLEATLVKDLSAAAGK
CCCCCCEEEEECEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
PVGVRVRSLKDVEAEGVVALPGVCIDDLDISVSDYEHPRPELLRCDDSRVVEESVHSHLL
CCEEEEECCCCCCCCCEEECCCEEECCCCCEECCCCCCCHHHEECCCHHHHHHHHHHHHH
KSNCPVTSQPDWGSVVVEYRGAALDHASLLEYIVSFRQHSDFHEQCVERIFLDLQRLLKP
HCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EKLTVFARYVRRGGLDINPYRSTESVQLPNHRLVRQ
HHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA