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The map label for this gene is yfbT [H]

Identifier: 77459750

GI number: 77459750

Start: 4006626

End: 4007294

Strand: Reverse

Name: yfbT [H]

Synonym: Pfl01_3528

Alternate gene names: 77459750

Gene position: 4007294-4006626 (Counterclockwise)

Preceding gene: 77459751

Following gene: 77459748

Centisome position: 62.24

GC content: 64.42

Gene sequence:

>669_bases
TTGTCTGCTCACGATGCTGTTTTCAACCGCGCGTTCGGCGCGTTCCTGTTCGACATGGACGGCACCGTCCTCAATTCCAT
CGCCGCCGCCGAGCGGATCTGGTCCGCCTGGGCCGTGCGCCACGGGGTGAACGTCGAAACGTTCCTGCCGACCATCCATG
GCGTGCGCGCCATCGACACCATCACGCGTTTGAACCTGCCGGGCGTGGACGCCGAGGCGCAAGCGGCGTTCATCACCGAG
GCGGAAATCGAAGACGTCGAAGGCATCGTCGAGATCCCCGGCGCGGCAGCGTTTCTCAAATCGTTGCCGGCGGATCGCTG
GGCCATGGTGACCTCGGCGCCACGGGATCTGGCGTTGCGGCGGATGGGCGCGGCGGGGATTCCTGAACCGGCGGTGATGA
TCACCGCTGAAGATGTGACCGCTGGCAAACCGGATCCGGCCGGTTATCGTCTGGCGGCCAGACGCCTGGGGCTGGAGCCG
GCGGATTGCCTGATTTTCGAAGACGCCACTGTCGGTATTCAGGCCGCTGAAGCCGCGGGTGCGCCGTTGATGATCATCAC
CACGACTCACCAGCATCCGCTCGAAACCGCCCACGCGACCATCGCCAGTTACCGCGACATTGCGCTGAGCATCGACAGCG
ACGGCCAGTTGCGTCTGCAACCTCAATAA

Upstream 100 bases:

>100_bases
CGGTGCGTTCAAATCCAAGGCGCCGCGTCTGCACTGAAGCATCCCGCAGGTGCTACATTGCCGGGCATCGTTGCCCGGCC
CCTTGCGAGGAAGTGATCTT

Downstream 100 bases:

>100_bases
ACCCCGGGCAGCAATCGCCAGCTGCGGGCGCAATAAGCGTCGTATTCGGCGCCGAACTGCGTGCGCAGCAAGGCCTCTTC
GGAGTGGATGCGGGCGATCA

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSAHDAVFNRAFGAFLFDMDGTVLNSIAAAERIWSAWAVRHGVNVETFLPTIHGVRAIDTITRLNLPGVDAEAQAAFITE
AEIEDVEGIVEIPGAAAFLKSLPADRWAMVTSAPRDLALRRMGAAGIPEPAVMITAEDVTAGKPDPAGYRLAARRLGLEP
ADCLIFEDATVGIQAAEAAGAPLMIITTTHQHPLETAHATIASYRDIALSIDSDGQLRLQPQ

Sequences:

>Translated_222_residues
MSAHDAVFNRAFGAFLFDMDGTVLNSIAAAERIWSAWAVRHGVNVETFLPTIHGVRAIDTITRLNLPGVDAEAQAAFITE
AEIEDVEGIVEIPGAAAFLKSLPADRWAMVTSAPRDLALRRMGAAGIPEPAVMITAEDVTAGKPDPAGYRLAARRLGLEP
ADCLIFEDATVGIQAAEAAGAPLMIITTTHQHPLETAHATIASYRDIALSIDSDGQLRLQPQ
>Mature_221_residues
SAHDAVFNRAFGAFLFDMDGTVLNSIAAAERIWSAWAVRHGVNVETFLPTIHGVRAIDTITRLNLPGVDAEAQAAFITEA
EIEDVEGIVEIPGAAAFLKSLPADRWAMVTSAPRDLALRRMGAAGIPEPAVMITAEDVTAGKPDPAGYRLAARRLGLEPA
DCLIFEDATVGIQAAEAAGAPLMIITTTHQHPLETAHATIASYRDIALSIDSDGQLRLQPQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI87082080, Length=211, Percent_Identity=37.914691943128, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI1789046, Length=175, Percent_Identity=29.7142857142857, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321834, Length=200, Percent_Identity=31.5, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321833, Length=197, Percent_Identity=32.48730964467, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320905, Length=185, Percent_Identity=32.4324324324324, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322136, Length=188, Percent_Identity=32.4468085106383, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23604; Mature: 23473

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHDAVFNRAFGAFLFDMDGTVLNSIAAAERIWSAWAVRHGVNVETFLPTIHGVRAIDT
CCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ITRLNLPGVDAEAQAAFITEAEIEDVEGIVEIPGAAAFLKSLPADRWAMVTSAPRDLALR
HHHCCCCCCCCCCCEEEEECCHHHHCCCEEECCCHHHHHHHCCCCCEEEECCCCHHHHHH
RMGAAGIPEPAVMITAEDVTAGKPDPAGYRLAARRLGLEPADCLIFEDATVGIQAAEAAG
HHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEHHCCC
APLMIITTTHQHPLETAHATIASYRDIALSIDSDGQLRLQPQ
CCEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCEEEECCC
>Mature Secondary Structure 
SAHDAVFNRAFGAFLFDMDGTVLNSIAAAERIWSAWAVRHGVNVETFLPTIHGVRAIDT
CCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ITRLNLPGVDAEAQAAFITEAEIEDVEGIVEIPGAAAFLKSLPADRWAMVTSAPRDLALR
HHHCCCCCCCCCCCEEEEECCHHHHCCCEEECCCHHHHHHHCCCCCEEEECCCCHHHHHH
RMGAAGIPEPAVMITAEDVTAGKPDPAGYRLAARRLGLEPADCLIFEDATVGIQAAEAAG
HHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEHHCCC
APLMIITTTHQHPLETAHATIASYRDIALSIDSDGQLRLQPQ
CCEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123 [H]