Click here to switch to the map view.

The map label for this gene is ilvA [H]

Identifier: 77459748

GI number: 77459748

Start: 4004923

End: 4005840

Strand: Reverse

Name: ilvA [H]

Synonym: Pfl01_3526

Alternate gene names: 77459748

Gene position: 4005840-4004923 (Counterclockwise)

Preceding gene: 77459750

Following gene: 77459745

Centisome position: 62.22

GC content: 66.12

Gene sequence:

>918_bases
ATGTTGCACATCCGCACGCCGCTGATCCTCCATCCGACCCTGTCGACTGCGTCCCGGCGCATGTGGCTGAAGCTGGAAAA
CCTGCAACCCTGTGGCTCGTTCAAGCTGCGCGGGATGGGCTTGTTGTGCAGTCAGGCAGCGGCGCAGGGCAAGCGCAAGG
TCGTATGCCCGTCCGGCGGCAATGCCGGGCTGGCCACGGCGGTGGCAGCGGTCAGCCTGGGTTTGCAAGCCTGCATCGTG
GTGCCGCACACCACGCCTGAAGCGACCCGCGCGCGTATTCGCCGCACCGGCGCGGACGTCATCGTCCACGGCAAAGTCTG
GGACGAGGCCAATCAGCGAGCGCGAGAACTGGCCAGCGCGGCGGACACCGAATACGTGCCGGCCTTCGATCACCCGGTGT
TGTGGGAAGGGCACAGCTCGATGGTCGACGAAATCCTCGACGACTGCCCGCAGGTCGATACCGTGGTCACGTCGGTCGGC
GGCGGTGGTTTGCTGGCGGGCATCCTCACCGGGCTGCTGCGCCATGACCGCCGAGACTGCCGCATCATCACCTGCGAAAC
CACAGGCGCCGCCTCGTTCGCTGCGGCCGTCCAGGCTGGCCATCCGGTGCGCCTGAGTCGGATCGACAGTGTCGCCACCT
CCCTCGGCGCCGCCCAGGTGGCGGCTTGGCCGGTAGAGCATATCGGCGAGTTCGATCATGAGTGTCTGGTGCTGTCAGAT
GACGACGCGATCATGGGCGTGGTGCGTTATGCCAGTGATCTGCGGCAATTGGTGGAGCCTGCGTGCGGGGTTTCGCTGGC
GGTGGCGTACCTCGATCATCCGGCGTTGGCGGGGGCGAATGATGTGGTGGTCATTGTTTGCGGCGGGGTGAGTATCAGTG
CGCAACTGGTGGCGGGGTGGGCGCGGTTGGGATCTTGA

Upstream 100 bases:

>100_bases
TCCCGGCACGATCCCCGGCGTTTAGCCGCTGGCAGGGGCGTGGACTAAACTTCAGGCAGAGACGTTCCGATCATCCGATC
CGCCGCTTCGGGAGTCCGCC

Downstream 100 bases:

>100_bases
ATGCTCAGGAGTTCCGCGTAGACCGCTTGCCCTCACCCCAGCCCTCTCCCGGAGGGAGAGGGAGCCGACCGAGGTGTCTT
GAGAGGTACATCGACCTGAT

Product: pyridoxal-5'-phosphate-dependent enzyme subunit beta

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MLHIRTPLILHPTLSTASRRMWLKLENLQPCGSFKLRGMGLLCSQAAAQGKRKVVCPSGGNAGLATAVAAVSLGLQACIV
VPHTTPEATRARIRRTGADVIVHGKVWDEANQRARELASAADTEYVPAFDHPVLWEGHSSMVDEILDDCPQVDTVVTSVG
GGGLLAGILTGLLRHDRRDCRIITCETTGAASFAAAVQAGHPVRLSRIDSVATSLGAAQVAAWPVEHIGEFDHECLVLSD
DDAIMGVVRYASDLRQLVEPACGVSLAVAYLDHPALAGANDVVVIVCGGVSISAQLVAGWARLGS

Sequences:

>Translated_305_residues
MLHIRTPLILHPTLSTASRRMWLKLENLQPCGSFKLRGMGLLCSQAAAQGKRKVVCPSGGNAGLATAVAAVSLGLQACIV
VPHTTPEATRARIRRTGADVIVHGKVWDEANQRARELASAADTEYVPAFDHPVLWEGHSSMVDEILDDCPQVDTVVTSVG
GGGLLAGILTGLLRHDRRDCRIITCETTGAASFAAAVQAGHPVRLSRIDSVATSLGAAQVAAWPVEHIGEFDHECLVLSD
DDAIMGVVRYASDLRQLVEPACGVSLAVAYLDHPALAGANDVVVIVCGGVSISAQLVAGWARLGS
>Mature_305_residues
MLHIRTPLILHPTLSTASRRMWLKLENLQPCGSFKLRGMGLLCSQAAAQGKRKVVCPSGGNAGLATAVAAVSLGLQACIV
VPHTTPEATRARIRRTGADVIVHGKVWDEANQRARELASAADTEYVPAFDHPVLWEGHSSMVDEILDDCPQVDTVVTSVG
GGGLLAGILTGLLRHDRRDCRIITCETTGAASFAAAVQAGHPVRLSRIDSVATSLGAAQVAAWPVEHIGEFDHECLVLSD
DDAIMGVVRYASDLRQLVEPACGVSLAVAYLDHPALAGANDVVVIVCGGVSISAQLVAGWARLGS

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI19923959, Length=306, Percent_Identity=42.156862745098, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI33469958, Length=315, Percent_Identity=42.5396825396825, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI11345492, Length=307, Percent_Identity=26.3843648208469, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1790207, Length=265, Percent_Identity=30.9433962264151, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789505, Length=138, Percent_Identity=28.9855072463768, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508781, Length=307, Percent_Identity=27.0358306188925, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71991565, Length=288, Percent_Identity=30.5555555555556, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319788, Length=318, Percent_Identity=37.4213836477987, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322024, Length=199, Percent_Identity=36.1809045226131, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320930, Length=282, Percent_Identity=25.531914893617, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322631, Length=287, Percent_Identity=26.4808362369338, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322023, Length=119, Percent_Identity=42.0168067226891, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355833, Length=286, Percent_Identity=29.020979020979, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 32028; Mature: 32028

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHIRTPLILHPTLSTASRRMWLKLENLQPCGSFKLRGMGLLCSQAAAQGKRKVVCPSGG
CEEECCCEEEECCHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCEEECCCCC
NAGLATAVAAVSLGLQACIVVPHTTPEATRARIRRTGADVIVHGKVWDEANQRARELASA
CCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHH
ADTEYVPAFDHPVLWEGHSSMVDEILDDCPQVDTVVTSVGGGGLLAGILTGLLRHDRRDC
CCCCCCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
RIITCETTGAASFAAAVQAGHPVRLSRIDSVATSLGAAQVAAWPVEHIGEFDHECLVLSD
EEEEEECCCCHHHHHHHHCCCCEEHHHHHHHHHHHCHHHHHHCCHHHHCCCCCCEEEECC
DDAIMGVVRYASDLRQLVEPACGVSLAVAYLDHPALAGANDVVVIVCGGVSISAQLVAGW
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHH
ARLGS
HHHCC
>Mature Secondary Structure
MLHIRTPLILHPTLSTASRRMWLKLENLQPCGSFKLRGMGLLCSQAAAQGKRKVVCPSGG
CEEECCCEEEECCHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCEEECCCCC
NAGLATAVAAVSLGLQACIVVPHTTPEATRARIRRTGADVIVHGKVWDEANQRARELASA
CCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHH
ADTEYVPAFDHPVLWEGHSSMVDEILDDCPQVDTVVTSVGGGGLLAGILTGLLRHDRRDC
CCCCCCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
RIITCETTGAASFAAAVQAGHPVRLSRIDSVATSLGAAQVAAWPVEHIGEFDHECLVLSD
EEEEEECCCCHHHHHHHHCCCCEEHHHHHHHHHHHCHHHHHHCCHHHHCCCCCCEEEECC
DDAIMGVVRYASDLRQLVEPACGVSLAVAYLDHPALAGANDVVVIVCGGVSISAQLVAGW
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHH
ARLGS
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]