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The map label for this gene is pucI [H]

Identifier: 77459662

GI number: 77459662

Start: 3922813

End: 3924297

Strand: Reverse

Name: pucI [H]

Synonym: Pfl01_3440

Alternate gene names: 77459662

Gene position: 3924297-3922813 (Counterclockwise)

Preceding gene: 77459666

Following gene: 77459661

Centisome position: 60.95

GC content: 61.89

Gene sequence:

>1485_bases
ATGCAACAGAACAGATCGCAAGTGACCGAGCGCGACGGCTTGTTCGAACTCGAAGCCGGCAGCGACGTCCTCGACAGTCC
CCGTTACAACCACGACATGGCACCGACCAAGGTGCGCGAACGAACCTGGAATAAGTGGCACATCACCGCGCTGTGGGTCG
GCATGTCGATCTGCGTGCCGACCTACACCCTCGGCGGCGTGCTCACCGCGTATTTCGGCCTGAGCGTCGGCGAAGCCTTG
CTGGCGATTCTGTTTGCCAACGTCATCGTCCTGATTCCTCTCACGCTCAATGCGTTTCCCGGCACCAAGTACGGGATTCC
GTTTCCGGTGCTGCTGCGATCGTCGTTCGGGATTCTCGGCTCCAACGTGCCGTGCCTGATCCGGGCGCTGGTGGCGTGCG
GCTGGTTCGGCATTCAGACCATGTTCGGCGGGCTGGCGATTCACCTGTTTCTCGGGTCGGTGTTCGACGGCTGGAAATCC
CTCGGCGGGACGGGGGAGGTGATCGGTTTCATGGTCTTCTGGGCGCTCAACCTGTGGGTGGTGATCCGCGGCGCCGAGTC
GATCAAATGGCTGGAAACCCTGTCGGCACCGCTGCTGGTGGCGGTCGGCATCGGGCTGTTGGTGTGGGCCATGCCGAATG
TGTCGATGACTGAATTGCTGGCGATTCCGCCGAAGCGTCCCGAAGGTGCGAGCGTGGTCAGTTACTTCGCCGCCGGGCTG
ACGGCGATGGTCGGTTTCTGGGCCACCCTGTCGCTGAACATTCCCGACTTCAGCCGCTATGCCCGCAGCCAGAAGGATCA
GATCCTCGGGCAGATTTTCGGTCTGCCGCTGACCATGTTCCTGTTTGCCGCACTGGGCGTGGTGATGACCGCCGCTTCGG
TGAAACTGGTGGGCGTCAGCGTCTCCGATCCGGTGACGTTGATCGGCCATATCCAGAGCCCGGTGTGGGTCGCGGTGGCC
ATGGCGCTGATCATCATCGCCACGCTGTCGACCAACACCGCCGCCAACATTGTTTCGCCAACCAACGACTTCCAGAACAT
CGCGCCCAAGGTGATCAACCGTACCAAAGCGGTAATCCTCACCGGGTTCGTCGGGCTGGCACTGATGGGCCATGAGCTGC
TGAAGAAGCTCGGCCTGATCGTCTCCGATGTCAGTCTGGAAACCGTGTATTCGAACTGGCTGCTGGGCTATTCCAGCCTG
CTCGGGCCGATTGCCGGGATCATGGTGGTGGACTATTTCCTGATCAAGAAACAGCAACTGGACCTGGCCGGCCTGTACCG
CGACGACGTGTACCCGGCGTGGAACTGGTCCGGGTTCATCGCCTTCGGCGTGCCGGTGGTGCTGACCCTGCTGTCGCTGG
GCAGCAGCGCATTCAGCTGGTTCTACAGCTACGGCTGGTTCACCGGCTCGGCGCTGGGCGGGTTGATCTATTACGGGTTG
TGCGCGATGCGTGCGCAGCCGGTGGCCGTCAAATCGCCGTTGTAA

Upstream 100 bases:

>100_bases
GCATGAAAGTTGTCAGTTCCTCCGGCCATCGTCCGGCTCGCACCTGCGTGTGCAGCCGCCTGATGAGCAAAAAAATAATC
AGAAGAACAGTGGAGCGGCC

Downstream 100 bases:

>100_bases
TTCCCATAAGAACTGCCTGAGGAGATCCCCATGAACGCAGCCGTAGACGTTCTGCAATCGACCCATCAGCACATCAACCG
CGACCGTTTGTGGGCGTCGC

Product: cytosine/purines, uracil, thiamine, allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MQQNRSQVTERDGLFELEAGSDVLDSPRYNHDMAPTKVRERTWNKWHITALWVGMSICVPTYTLGGVLTAYFGLSVGEAL
LAILFANVIVLIPLTLNAFPGTKYGIPFPVLLRSSFGILGSNVPCLIRALVACGWFGIQTMFGGLAIHLFLGSVFDGWKS
LGGTGEVIGFMVFWALNLWVVIRGAESIKWLETLSAPLLVAVGIGLLVWAMPNVSMTELLAIPPKRPEGASVVSYFAAGL
TAMVGFWATLSLNIPDFSRYARSQKDQILGQIFGLPLTMFLFAALGVVMTAASVKLVGVSVSDPVTLIGHIQSPVWVAVA
MALIIIATLSTNTAANIVSPTNDFQNIAPKVINRTKAVILTGFVGLALMGHELLKKLGLIVSDVSLETVYSNWLLGYSSL
LGPIAGIMVVDYFLIKKQQLDLAGLYRDDVYPAWNWSGFIAFGVPVVLTLLSLGSSAFSWFYSYGWFTGSALGGLIYYGL
CAMRAQPVAVKSPL

Sequences:

>Translated_494_residues
MQQNRSQVTERDGLFELEAGSDVLDSPRYNHDMAPTKVRERTWNKWHITALWVGMSICVPTYTLGGVLTAYFGLSVGEAL
LAILFANVIVLIPLTLNAFPGTKYGIPFPVLLRSSFGILGSNVPCLIRALVACGWFGIQTMFGGLAIHLFLGSVFDGWKS
LGGTGEVIGFMVFWALNLWVVIRGAESIKWLETLSAPLLVAVGIGLLVWAMPNVSMTELLAIPPKRPEGASVVSYFAAGL
TAMVGFWATLSLNIPDFSRYARSQKDQILGQIFGLPLTMFLFAALGVVMTAASVKLVGVSVSDPVTLIGHIQSPVWVAVA
MALIIIATLSTNTAANIVSPTNDFQNIAPKVINRTKAVILTGFVGLALMGHELLKKLGLIVSDVSLETVYSNWLLGYSSL
LGPIAGIMVVDYFLIKKQQLDLAGLYRDDVYPAWNWSGFIAFGVPVVLTLLSLGSSAFSWFYSYGWFTGSALGGLIYYGL
CAMRAQPVAVKSPL
>Mature_494_residues
MQQNRSQVTERDGLFELEAGSDVLDSPRYNHDMAPTKVRERTWNKWHITALWVGMSICVPTYTLGGVLTAYFGLSVGEAL
LAILFANVIVLIPLTLNAFPGTKYGIPFPVLLRSSFGILGSNVPCLIRALVACGWFGIQTMFGGLAIHLFLGSVFDGWKS
LGGTGEVIGFMVFWALNLWVVIRGAESIKWLETLSAPLLVAVGIGLLVWAMPNVSMTELLAIPPKRPEGASVVSYFAAGL
TAMVGFWATLSLNIPDFSRYARSQKDQILGQIFGLPLTMFLFAALGVVMTAASVKLVGVSVSDPVTLIGHIQSPVWVAVA
MALIIIATLSTNTAANIVSPTNDFQNIAPKVINRTKAVILTGFVGLALMGHELLKKLGLIVSDVSLETVYSNWLLGYSSL
LGPIAGIMVVDYFLIKKQQLDLAGLYRDDVYPAWNWSGFIAFGVPVVLTLLSLGSSAFSWFYSYGWFTGSALGGLIYYGL
CAMRAQPVAVKSPL

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=467, Percent_Identity=27.4089935760171, Blast_Score=157, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322219, Length=422, Percent_Identity=24.8815165876777, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319495, Length=425, Percent_Identity=23.7647058823529, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319429, Length=479, Percent_Identity=22.7557411273486, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324645, Length=418, Percent_Identity=22.9665071770335, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323266, Length=418, Percent_Identity=23.444976076555, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324766, Length=414, Percent_Identity=21.7391304347826, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53346; Mature: 53346

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQNRSQVTERDGLFELEAGSDVLDSPRYNHDMAPTKVRERTWNKWHITALWVGMSICVP
CCCCHHHHHHHCCCEEECCCCCHHCCCCCCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHH
TYTLGGVLTAYFGLSVGEALLAILFANVIVLIPLTLNAFPGTKYGIPFPVLLRSSFGILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCC
SNVPCLIRALVACGWFGIQTMFGGLAIHLFLGSVFDGWKSLGGTGEVIGFMVFWALNLWV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VIRGAESIKWLETLSAPLLVAVGIGLLVWAMPNVSMTELLAIPPKRPEGASVVSYFAAGL
EEECCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHH
TAMVGFWATLSLNIPDFSRYARSQKDQILGQIFGLPLTMFLFAALGVVMTAASVKLVGVS
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
VSDPVTLIGHIQSPVWVAVAMALIIIATLSTNTAANIVSPTNDFQNIAPKVINRTKAVIL
CCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TGFVGLALMGHELLKKLGLIVSDVSLETVYSNWLLGYSSLLGPIAGIMVVDYFLIKKQQL
HHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLAGLYRDDVYPAWNWSGFIAFGVPVVLTLLSLGSSAFSWFYSYGWFTGSALGGLIYYGL
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
CAMRAQPVAVKSPL
HHHCCCCCCCCCCC
>Mature Secondary Structure
MQQNRSQVTERDGLFELEAGSDVLDSPRYNHDMAPTKVRERTWNKWHITALWVGMSICVP
CCCCHHHHHHHCCCEEECCCCCHHCCCCCCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHH
TYTLGGVLTAYFGLSVGEALLAILFANVIVLIPLTLNAFPGTKYGIPFPVLLRSSFGILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCC
SNVPCLIRALVACGWFGIQTMFGGLAIHLFLGSVFDGWKSLGGTGEVIGFMVFWALNLWV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VIRGAESIKWLETLSAPLLVAVGIGLLVWAMPNVSMTELLAIPPKRPEGASVVSYFAAGL
EEECCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHH
TAMVGFWATLSLNIPDFSRYARSQKDQILGQIFGLPLTMFLFAALGVVMTAASVKLVGVS
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
VSDPVTLIGHIQSPVWVAVAMALIIIATLSTNTAANIVSPTNDFQNIAPKVINRTKAVIL
CCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TGFVGLALMGHELLKKLGLIVSDVSLETVYSNWLLGYSSLLGPIAGIMVVDYFLIKKQQL
HHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLAGLYRDDVYPAWNWSGFIAFGVPVVLTLLSLGSSAFSWFYSYGWFTGSALGGLIYYGL
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
CAMRAQPVAVKSPL
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]