Click here to switch to the map view.

The map label for this gene is rutR [H]

Identifier: 77459666

GI number: 77459666

Start: 3929505

End: 3930125

Strand: Reverse

Name: rutR [H]

Synonym: Pfl01_3444

Alternate gene names: 77459666

Gene position: 3930125-3929505 (Counterclockwise)

Preceding gene: 77459670

Following gene: 77459662

Centisome position: 61.04

GC content: 58.45

Gene sequence:

>621_bases
ATGGGCAATCACAAGATCGAAATCCGCCGCACCAACGTCGACAAGATCCTGCTGGCGGCGGAAAAAGTCTTCGCCGAAAA
AGGTTTCGGCGGCACCGCCATGGCGGACATCGCCGCTGAAGTGCAGCTGCCGCGTTCCAACCTGCATTACTACTTCTCGA
CCAAGAGCGAGCTGTACAGCGCGGTGCTGTTCGACCTGCTGGAAGTGTGGAAGCAGGACGCGCTGTGCTTCGAGATGTTC
GACGATCCGCGCGTGGTGCTCAGCAGCTACATCCGCGCCAAGATGAACCACTCGCGCAGCCGGCCTTACGGGTCGAAAGT
CTGGGCCAACGAAATCATCCACGGCGCCCCGACCCTCGGCGAGGCGCTGGATGTGAGCCTGTATGACTGGGCGAAGATGA
AAGAGGCGAAGATCCGCCAGTGGGTTGAGGACAAGCGGATTCTGCCGGTGGAGCCGTCGAGTTTGTTGTATATGATCTGG
GCGTCGACGCAGCACTATGCCGACTTTGATCATCAGGTGAATATTTTGAATGAGCATCAGCCGCTGTCGGATATGCAGTT
CGAGCGGGCGGTGCAGACGGTGACTAGCGTGATTTTGCGGGGGATTGGGTTGGAGCCTTGA

Upstream 100 bases:

>100_bases
ACCAGAAAAAAGCCCAAGTGTGTGAAGAATCGTTCTCGGCTGTCGCCCCGTGCCCAGAGCGGATGCTAGTCTTCAACAAC
GTTGTCTTCAGGGAGCCCTC

Downstream 100 bases:

>100_bases
AAGTCAAAAGCCTGCCCTCACCCCAGCCCTCTCCCGGAGGGAGAGGGGGCCTACCGAGGTGTCTTGCGGGTTACATCGAC
CTGAACCACCGAGTTGATTA

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MGNHKIEIRRTNVDKILLAAEKVFAEKGFGGTAMADIAAEVQLPRSNLHYYFSTKSELYSAVLFDLLEVWKQDALCFEMF
DDPRVVLSSYIRAKMNHSRSRPYGSKVWANEIIHGAPTLGEALDVSLYDWAKMKEAKIRQWVEDKRILPVEPSSLLYMIW
ASTQHYADFDHQVNILNEHQPLSDMQFERAVQTVTSVILRGIGLEP

Sequences:

>Translated_206_residues
MGNHKIEIRRTNVDKILLAAEKVFAEKGFGGTAMADIAAEVQLPRSNLHYYFSTKSELYSAVLFDLLEVWKQDALCFEMF
DDPRVVLSSYIRAKMNHSRSRPYGSKVWANEIIHGAPTLGEALDVSLYDWAKMKEAKIRQWVEDKRILPVEPSSLLYMIW
ASTQHYADFDHQVNILNEHQPLSDMQFERAVQTVTSVILRGIGLEP
>Mature_205_residues
GNHKIEIRRTNVDKILLAAEKVFAEKGFGGTAMADIAAEVQLPRSNLHYYFSTKSELYSAVLFDLLEVWKQDALCFEMFD
DPRVVLSSYIRAKMNHSRSRPYGSKVWANEIIHGAPTLGEALDVSLYDWAKMKEAKIRQWVEDKRILPVEPSSLLYMIWA
STQHYADFDHQVNILNEHQPLSDMQFERAVQTVTSVILRGIGLEP

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=187, Percent_Identity=36.8983957219251, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 23616; Mature: 23485

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNHKIEIRRTNVDKILLAAEKVFAEKGFGGTAMADIAAEVQLPRSNLHYYFSTKSELYS
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHH
AVLFDLLEVWKQDALCFEMFDDPRVVLSSYIRAKMNHSRSRPYGSKVWANEIIHGAPTLG
HHHHHHHHHHHHCCEEEHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCC
EALDVSLYDWAKMKEAKIRQWVEDKRILPVEPSSLLYMIWASTQHYADFDHQVNILNEHQ
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCC
PLSDMQFERAVQTVTSVILRGIGLEP
CCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GNHKIEIRRTNVDKILLAAEKVFAEKGFGGTAMADIAAEVQLPRSNLHYYFSTKSELYS
CCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHH
AVLFDLLEVWKQDALCFEMFDDPRVVLSSYIRAKMNHSRSRPYGSKVWANEIIHGAPTLG
HHHHHHHHHHHHCCEEEHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCC
EALDVSLYDWAKMKEAKIRQWVEDKRILPVEPSSLLYMIWASTQHYADFDHQVNILNEHQ
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCC
PLSDMQFERAVQTVTSVILRGIGLEP
CCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]