Click here to switch to the map view.

The map label for this gene is moaA

Identifier: 77459610

GI number: 77459610

Start: 3864737

End: 3865735

Strand: Direct

Name: moaA

Synonym: Pfl01_3388

Alternate gene names: 77459610

Gene position: 3864737-3865735 (Clockwise)

Preceding gene: 77459606

Following gene: 77459611

Centisome position: 60.03

GC content: 62.16

Gene sequence:

>999_bases
ATGTCAGAGCGCGTCTTGATCGATGGTTACAACCGCCGCGTCGACTACCTGCGCATGTCGGTCACCGACCGCTGCGACTT
TCGCTGCGTGTATTGCATGGCCGAAGACATGCAGTTTCTGCCGCGCCAACGGGTGCTGACGCTGGAGGAAATCTATCAAC
TGGCGCAGAGCTTCGTTGCGCTCGGCACCCGCAAGATTCGCCTGACCGGCGGCGAGCCGCTGATTCGTCCCGGCGTCGTC
GGCTTGTGCAAGCAGATCGCCGCCCTGCCCGGCCTGCGCGAACTGTGCCTGACCACCAACGGCTCGCAGCTCGGCAAACT
GGCCAGCCCGCTGTTCGACGCCGGGGTCAAACGCCTCAACGTCAGCCTCGACAGTCTTGATGCCGAGCGTTTCAAGCAGA
TGACCCGCACCGGTGATCTGGCCCAGGTGATCGATGGCATCGACGCCGCGCGCGCCGCCGGATTTACCCGCACTAAACTC
AACTGCGTGGTGATGCAGGGCCGCAACGATCACGAGATCAACGATCTGGTGCAGTTCGCCATCGAACGGGATCTGGACAT
TTCCTTCATCGAGGAAATGCCGCTGGGGATCATCAGCGAACACAGCCGCGCCGAGTCGTTCTTCTCCAGTACCCAAGTGC
GCGAACGGATCGCCGAGCGCTACACCCTGATCGACTCCGCCGAGTCGACCCATGGCCCGTCGCGCTACTGGCGGCTCGCC
GAAGCGCCGCACATTCGGCTGGGATTCATCTCGCCCCACAGCCACAACTTCTGCGGCACCTGCAACCGGGTGCGGCTGAC
CGTCGAGGGGCGTTTGCTACTGTGTCTGGGGAACGAGCATTCGGTGGATCTGAAAGCGGTGCTGCGGGCCCACCCGGGTC
AACCGGAGCGGCTTGAGAAAGCCATCATCGAAGCGATGAAACTCAAGCCTTACCGGCACAACTTTGAAGTGAACGACGAC
GTGCAAGTGGTGCGTTTCATGAACATGACCGGCGGCTGA

Upstream 100 bases:

>100_bases
TCGGCCGGTCATTAAGAGTGATTGGACTCTGTCTCAAGTTGCTCATAACCTGCGCCGACCCAATCCCTTCAATAGCGGAT
TTCACCCAAGCGAGGCTGTC

Downstream 100 bases:

>100_bases
TCCGCCACGTTTGCAAGGCAGAGAGCCGCATGATCATCCGACCCAAGGTCAATCAATTCGCCATTCTCTTCACCCTCAAG
GGCTCGATTGCCAAGCGCAT

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSERVLIDGYNRRVDYLRMSVTDRCDFRCVYCMAEDMQFLPRQRVLTLEEIYQLAQSFVALGTRKIRLTGGEPLIRPGVV
GLCKQIAALPGLRELCLTTNGSQLGKLASPLFDAGVKRLNVSLDSLDAERFKQMTRTGDLAQVIDGIDAARAAGFTRTKL
NCVVMQGRNDHEINDLVQFAIERDLDISFIEEMPLGIISEHSRAESFFSSTQVRERIAERYTLIDSAESTHGPSRYWRLA
EAPHIRLGFISPHSHNFCGTCNRVRLTVEGRLLLCLGNEHSVDLKAVLRAHPGQPERLEKAIIEAMKLKPYRHNFEVNDD
VQVVRFMNMTGG

Sequences:

>Translated_332_residues
MSERVLIDGYNRRVDYLRMSVTDRCDFRCVYCMAEDMQFLPRQRVLTLEEIYQLAQSFVALGTRKIRLTGGEPLIRPGVV
GLCKQIAALPGLRELCLTTNGSQLGKLASPLFDAGVKRLNVSLDSLDAERFKQMTRTGDLAQVIDGIDAARAAGFTRTKL
NCVVMQGRNDHEINDLVQFAIERDLDISFIEEMPLGIISEHSRAESFFSSTQVRERIAERYTLIDSAESTHGPSRYWRLA
EAPHIRLGFISPHSHNFCGTCNRVRLTVEGRLLLCLGNEHSVDLKAVLRAHPGQPERLEKAIIEAMKLKPYRHNFEVNDD
VQVVRFMNMTGG
>Mature_331_residues
SERVLIDGYNRRVDYLRMSVTDRCDFRCVYCMAEDMQFLPRQRVLTLEEIYQLAQSFVALGTRKIRLTGGEPLIRPGVVG
LCKQIAALPGLRELCLTTNGSQLGKLASPLFDAGVKRLNVSLDSLDAERFKQMTRTGDLAQVIDGIDAARAAGFTRTKLN
CVVMQGRNDHEINDLVQFAIERDLDISFIEEMPLGIISEHSRAESFFSSTQVRERIAERYTLIDSAESTHGPSRYWRLAE
APHIRLGFISPHSHNFCGTCNRVRLTVEGRLLLCLGNEHSVDLKAVLRAHPGQPERLEKAIIEAMKLKPYRHNFEVNDDV
QVVRFMNMTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=294, Percent_Identity=36.734693877551, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI22027536, Length=328, Percent_Identity=35.3658536585366, Blast_Score=192, Evalue=4e-49,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=33.434650455927, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI32566329, Length=311, Percent_Identity=35.6913183279743, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI32566331, Length=311, Percent_Identity=35.6913183279743, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17561210, Length=311, Percent_Identity=36.6559485530547, Blast_Score=204, Evalue=7e-53,
Organism=Drosophila melanogaster, GI28574488, Length=330, Percent_Identity=39.0909090909091, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI28574490, Length=311, Percent_Identity=39.871382636656, Blast_Score=206, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_PSEPF (Q3KAS8)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_349117.1
- HSSP:   P69848
- ProteinModelPortal:   Q3KAS8
- SMR:   Q3KAS8
- STRING:   Q3KAS8
- GeneID:   3713338
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_3388
- eggNOG:   COG2896
- HOGENOM:   HBG529885
- OMA:   PLVRRNM
- ProtClustDB:   PRK00164
- BioCyc:   PFLU205922:PFL_3388-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 37511; Mature: 37380

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 18-18 BINDING 31-31 BINDING 67-67 BINDING 71-71 BINDING 98-98 BINDING 122-122 BINDING 159-159 BINDING 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERVLIDGYNRRVDYLRMSVTDRCDFRCVYCMAEDMQFLPRQRVLTLEEIYQLAQSFVA
CCCCEEECCCCCCEEEEEEECCCCCCCEEEEEEHHHHHHCCHHHCCCHHHHHHHHHHHHH
LGTRKIRLTGGEPLIRPGVVGLCKQIAALPGLRELCLTTNGSQLGKLASPLFDAGVKRLN
HCCEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEE
VSLDSLDAERFKQMTRTGDLAQVIDGIDAARAAGFTRTKLNCVVMQGRNDHEINDLVQFA
CCHHHCCHHHHHHHHCCCCHHHHHHCHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHH
IERDLDISFIEEMPLGIISEHSRAESFFSSTQVRERIAERYTLIDSAESTHGPSRYWRLA
HHCCCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECC
EAPHIRLGFISPHSHNFCGTCNRVRLTVEGRLLLCLGNEHSVDLKAVLRAHPGQPERLEK
CCCEEEEEEECCCCCCCCCCCCEEEEEECCEEEEEECCCCCCHHHHHHHCCCCCHHHHHH
AIIEAMKLKPYRHNFEVNDDVQVVRFMNMTGG
HHHHHHHCCCCCCCCCCCCCHHHHEEECCCCC
>Mature Secondary Structure 
SERVLIDGYNRRVDYLRMSVTDRCDFRCVYCMAEDMQFLPRQRVLTLEEIYQLAQSFVA
CCCEEECCCCCCEEEEEEECCCCCCCEEEEEEHHHHHHCCHHHCCCHHHHHHHHHHHHH
LGTRKIRLTGGEPLIRPGVVGLCKQIAALPGLRELCLTTNGSQLGKLASPLFDAGVKRLN
HCCEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEE
VSLDSLDAERFKQMTRTGDLAQVIDGIDAARAAGFTRTKLNCVVMQGRNDHEINDLVQFA
CCHHHCCHHHHHHHHCCCCHHHHHHCHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHH
IERDLDISFIEEMPLGIISEHSRAESFFSSTQVRERIAERYTLIDSAESTHGPSRYWRLA
HHCCCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECC
EAPHIRLGFISPHSHNFCGTCNRVRLTVEGRLLLCLGNEHSVDLKAVLRAHPGQPERLEK
CCCEEEEEEECCCCCCCCCCCCEEEEEECCEEEEEECCCCCCHHHHHHHCCCCCHHHHHH
AIIEAMKLKPYRHNFEVNDDVQVVRFMNMTGG
HHHHHHHCCCCCCCCCCCCCHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA