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The map label for this gene is rof [H]

Identifier: 77459509

GI number: 77459509

Start: 3773146

End: 3773424

Strand: Direct

Name: rof [H]

Synonym: Pfl01_3287

Alternate gene names: 77459509

Gene position: 3773146-3773424 (Clockwise)

Preceding gene: 77459507

Following gene: 77459511

Centisome position: 58.6

GC content: 55.56

Gene sequence:

>279_bases
ATGACCCGTTATCAGCCATTGAATTGTGACCTTTACGACTACCTGGAAATCGCTTGCCTGCGCGGCTATCAGCTCGACAT
CGAGTTGATCGACGGTCAGCGGCTGATGGCCAAAGCCGTGACCACGCGAACCAGCAGTGACAAAGAAGAATTCCTGGTGA
TCGAGCAACAAGCCGCGCAACAGGAAATTCGCCTCGACCAACTGCTTGCCATCACGCCTCAGGAGACCAACGCCGAGTTC
GGTCGGGTGATCCTTGCCGAAGGGGTTTGCGCAATCTGA

Upstream 100 bases:

>100_bases
CGATCCACGCCCTTCTCTGCCGCCATCAGCACCGCCGCATGAAGGTTGATGCATGACCTGGATCGTTTCATCATCGCCCA
AGGTTTCGAGGAGTAACGCC

Downstream 100 bases:

>100_bases
ACAAAAAATCCCGCTCGGAATCAACCGGCGGGATTTTTTGTGACACGTCGCTTTACGCGACGACGGGAATCACCGGATCC
GCAGCAGCCAGCGCAATCGC

Product: transcriptional antiterminator, Rof

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 92; Mature: 91

Protein sequence:

>92_residues
MTRYQPLNCDLYDYLEIACLRGYQLDIELIDGQRLMAKAVTTRTSSDKEEFLVIEQQAAQQEIRLDQLLAITPQETNAEF
GRVILAEGVCAI

Sequences:

>Translated_92_residues
MTRYQPLNCDLYDYLEIACLRGYQLDIELIDGQRLMAKAVTTRTSSDKEEFLVIEQQAAQQEIRLDQLLAITPQETNAEF
GRVILAEGVCAI
>Mature_91_residues
TRYQPLNCDLYDYLEIACLRGYQLDIELIDGQRLMAKAVTTRTSSDKEEFLVIEQQAAQQEIRLDQLLAITPQETNAEFG
RVILAEGVCAI

Specific function: Suppresses temperature-sensitive mutations in essential genes by modulating rho-dependent transcription termination [H]

COG id: COG4568

COG function: function code K; Transcriptional antiterminator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081698, Length=85, Percent_Identity=40, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002730
- InterPro:   IPR009778 [H]

Pfam domain/function: PF07073 ROF [H]

EC number: NA

Molecular weight: Translated: 10467; Mature: 10336

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYQPLNCDLYDYLEIACLRGYQLDIELIDGQRLMAKAVTTRTSSDKEEFLVIEQQAAQ
CCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCCCCEEEEEHHHHH
QEIRLDQLLAITPQETNAEFGRVILAEGVCAI
HHHCHHHEEEECCCCCCCCCCEEEECCCEECC
>Mature Secondary Structure 
TRYQPLNCDLYDYLEIACLRGYQLDIELIDGQRLMAKAVTTRTSSDKEEFLVIEQQAAQ
CCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCCCCEEEEEHHHHH
QEIRLDQLLAITPQETNAEFGRVILAEGVCAI
HHHCHHHEEEECCCCCCCCCCEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9723924; 9278503 [H]