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The map label for this gene is pulE [H]

Identifier: 77459396

GI number: 77459396

Start: 3654142

End: 3655548

Strand: Reverse

Name: pulE [H]

Synonym: Pfl01_3174

Alternate gene names: 77459396

Gene position: 3655548-3654142 (Counterclockwise)

Preceding gene: 77459397

Following gene: 77459395

Centisome position: 56.78

GC content: 65.81

Gene sequence:

>1407_bases
ATGAGCGCATTGCCTTACGCCTGGGCCAAGGCGCAGCGGATTCTGCTGCGCGACGGCGTGCTGACGGTGTGCCCGTCGAC
GCCGGGCTGGTCGATCAGCGAGGTGCGCCGGCAGTTCGGCGCGGCCAGGCTTGAGCGGGTGCGCGATGACGAACTCGACG
GCTTGCTCGCCACGGCTTACGCCGACACTGGCAGCGCGGCGGCGGTGGTCGGTGCGGCGGAAAACGAAGTCGACCTCGAT
CGCCTGATGCAGGACATGCCGGAAATCACTGACCTGCTCGACACCCAGGACGGCGCGCCGGTGATCCGCATGATCAACGC
CTTGCTCACCCAAGCGGCGCGGGACGAGGCCAGCGACATTCACATCGAACCGTTCGAAACCCATTCGGTGGTGCGCTACC
GGGTCGATGGCACCCTGCGCGACGTGGTCTCGCCGCGCAAGGCGTTGCACGGTGCGCTGGTGTCGCGGATCAAGATCATG
GCCCAGCTCGATATCGCCGAAAAACGCCTGCCGCAGGACGGGCGCATTGCGTTGCGCGTAGCAGGGCGGCCGATCGACAT
TCGCGTATCGACCGTGCCGACCGGCCATGGCGAGCGCGTGGTGATGCGTCTGCTGGACAAGCAGGCCGGGCGCCTGCATC
TGGAAACCCTGGGCATGGACGCGCAGGTGCTGGCCAAACTCGATCACCTGATCCGCCAGCCCCACGGCATTGTGCTGGTC
ACCGGCCCGACCGGCAGCGGCAAGACCACGAGTTTGTATGCGGCACTGGCGCGGCTCGATGCGAGCACCAGCAACATCCT
CACCGTGGAAGACCCGGTGGAGTACGACCTGCCGGGCATCAGCCAGATTCAGGTCAACGCCAAGATCGACATGACTTTTG
CCCTCGCGCTGCGTGCCATTTTGCGCCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTCGAAACCGCGCAG
ATCGCGGTGCAGGCGTCGCTGACCGGTCACCTGGTGCTCGCGACCCTGCACACCAACGACGCGGTCTCGGCGGTCAACCG
CTTGATCGACATGGGCGTCGAGCCGTTTTTGCTGGCCTCGTCAATGCTCGGGGTGTTGGCCCAGCGTTTGGTGCGGCGCC
TGTGCAATCAGTGCAAACAGGAAGATCCGGCCACGCCCGGCACCTGGCGCCCGGTCGGTTGCGCGGCGTGCAACCACACC
GGTTACAGCGGCCGGACCGGCATTCATGAATTGTTCTGCATCGATGACGACATCCGCACGCTGATTCACCAAGGGGCAGG
GGAGCAGGCTTTGCGCGCGGCGGCGAGCAAGGCCGGGATGTTCAGCCTGCGTGAGGACGGCGAGCGCTGGATTCGCAGCG
GTGCCACAGCCCCTGAAGAAATCCTTCGCGTGACACGGGACGCCTGA

Upstream 100 bases:

>100_bases
CGATGCCGGACATGCAGGCGCCGCAATTGCCGGCCACTGCCCAAGGCGTGCCGGCACCGGTGTCCGGCCCGCGTGCGACC
ATCAAAGCGGTGCCGCTGCA

Downstream 100 bases:

>100_bases
TGAATCGCTATCGTTTTGAAGCCGCCGATGCCAGCGGCAAGATCGAGTCCGGGCATCTGGAGGCGGACAGTCAGGGCGCG
GCGTTTGGCGTGTTGCGCGG

Product: type II secretion system protein E (GspE)

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSALPYAWAKAQRILLRDGVLTVCPSTPGWSISEVRRQFGAARLERVRDDELDGLLATAYADTGSAAAVVGAAENEVDLD
RLMQDMPEITDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGTLRDVVSPRKALHGALVSRIKIM
AQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLHLETLGMDAQVLAKLDHLIRQPHGIVLV
TGPTGSGKTTSLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNAKIDMTFALALRAILRQDPDIIMIGEIRDLETAQ
IAVQASLTGHLVLATLHTNDAVSAVNRLIDMGVEPFLLASSMLGVLAQRLVRRLCNQCKQEDPATPGTWRPVGCAACNHT
GYSGRTGIHELFCIDDDIRTLIHQGAGEQALRAAASKAGMFSLREDGERWIRSGATAPEEILRVTRDA

Sequences:

>Translated_468_residues
MSALPYAWAKAQRILLRDGVLTVCPSTPGWSISEVRRQFGAARLERVRDDELDGLLATAYADTGSAAAVVGAAENEVDLD
RLMQDMPEITDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGTLRDVVSPRKALHGALVSRIKIM
AQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLHLETLGMDAQVLAKLDHLIRQPHGIVLV
TGPTGSGKTTSLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNAKIDMTFALALRAILRQDPDIIMIGEIRDLETAQ
IAVQASLTGHLVLATLHTNDAVSAVNRLIDMGVEPFLLASSMLGVLAQRLVRRLCNQCKQEDPATPGTWRPVGCAACNHT
GYSGRTGIHELFCIDDDIRTLIHQGAGEQALRAAASKAGMFSLREDGERWIRSGATAPEEILRVTRDA
>Mature_467_residues
SALPYAWAKAQRILLRDGVLTVCPSTPGWSISEVRRQFGAARLERVRDDELDGLLATAYADTGSAAAVVGAAENEVDLDR
LMQDMPEITDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGTLRDVVSPRKALHGALVSRIKIMA
QLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLHLETLGMDAQVLAKLDHLIRQPHGIVLVT
GPTGSGKTTSLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNAKIDMTFALALRAILRQDPDIIMIGEIRDLETAQI
AVQASLTGHLVLATLHTNDAVSAVNRLIDMGVEPFLLASSMLGVLAQRLVRRLCNQCKQEDPATPGTWRPVGCAACNHTG
YSGRTGIHELFCIDDDIRTLIHQGAGEQALRAAASKAGMFSLREDGERWIRSGATAPEEILRVTRDA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=481, Percent_Identity=49.8960498960499, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1786296, Length=378, Percent_Identity=44.4444444444444, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=43.0769230769231, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 50868; Mature: 50737

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALPYAWAKAQRILLRDGVLTVCPSTPGWSISEVRRQFGAARLERVRDDELDGLLATAY
CCCCCCHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCEEEEEE
ADTGSAAAVVGAAENEVDLDRLMQDMPEITDLLDTQDGAPVIRMINALLTQAARDEASDI
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCE
HIEPFETHSVVRYRVDGTLRDVVSPRKALHGALVSRIKIMAQLDIAEKRLPQDGRIALRV
EECCCCCCCEEEEEECCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
AGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLHLETLGMDAQVLAKLDHLIRQPHGIVLV
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEE
TGPTGSGKTTSLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNAKIDMTFALALRAI
ECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEEECHHHHHHHHHH
LRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAVSAVNRLIDMGVEPFLLAS
HHCCCCEEEECCCCCCCHHEEEEEEECCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHH
SMLGVLAQRLVRRLCNQCKQEDPATPGTWRPVGCAACNHTGYSGRTGIHELFCIDDDIRT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECHHHHH
LIHQGAGEQALRAAASKAGMFSLREDGERWIRSGATAPEEILRVTRDA
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SALPYAWAKAQRILLRDGVLTVCPSTPGWSISEVRRQFGAARLERVRDDELDGLLATAY
CCCCCHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCEEEEEE
ADTGSAAAVVGAAENEVDLDRLMQDMPEITDLLDTQDGAPVIRMINALLTQAARDEASDI
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCE
HIEPFETHSVVRYRVDGTLRDVVSPRKALHGALVSRIKIMAQLDIAEKRLPQDGRIALRV
EECCCCCCCEEEEEECCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
AGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLHLETLGMDAQVLAKLDHLIRQPHGIVLV
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEE
TGPTGSGKTTSLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNAKIDMTFALALRAI
ECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEEECHHHHHHHHHH
LRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAVSAVNRLIDMGVEPFLLAS
HHCCCCEEEECCCCCCCHHEEEEEEECCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHH
SMLGVLAQRLVRRLCNQCKQEDPATPGTWRPVGCAACNHTGYSGRTGIHELFCIDDDIRT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECHHHHH
LIHQGAGEQALRAAASKAGMFSLREDGERWIRSGATAPEEILRVTRDA
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]