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The map label for this gene is epsD [H]

Identifier: 77459397

GI number: 77459397

Start: 3655545

End: 3657896

Strand: Reverse

Name: epsD [H]

Synonym: Pfl01_3175

Alternate gene names: 77459397

Gene position: 3657896-3655545 (Counterclockwise)

Preceding gene: 77459398

Following gene: 77459396

Centisome position: 56.81

GC content: 64.29

Gene sequence:

>2352_bases
ATGAAGGGGTCAGGGTCCAAACGCATGGCGTTGCCGATGCTGTTGATGGCATTGAGCGCGTGCAGCAACACCACACCACC
GAATCAACCGCCGCTGCTGGTGGACAGCGAACTCGGCCGGCCGCTGGCCAACACCCAGCGCAGTGGCGACGCGGTGCTCG
ACCGCGAGCGTGCGCAGGCTCAGGCAAGACCTGCGCCGAAGCAATTGCACAACATCACCGAACGCGCCCGCAGTGGCGGT
TCGGCGCGGGGCACGACGTTGCCGGCCAATCCGTTGGGCGATCAACCGGTGACGCTGAATTTTGTCGACGCCGATATCCA
AGCGGTGGTGCGGGCGTTGTCGCGCTCGACCGGTCAGCAGTTTCTGGTCGATCCACGGGTCAAGGGCAACCTGACGCTGG
TCTCCGAAGGTCAGGTGCCGGCGCATCAGGCCTACGACATGTTGCTGGCGGCGCTGCGCATGCAGGGTTTCAGCGTGGTC
GATGTCGGCGGCGTGGCGCAGGTGGTGCCGGAGGCCGACGCCAAATTGCTCGGCGGGCCGATCTACAGCGCCGACAAACC
GGCCGGCAACGGCATGCTCACCCGCACGTTCCGCCTGCAATACGAGAACGCCGTGAACCTGATCCCGGTGCTGCGCCCGA
TCGTGTCGCCGAACAATCCGATCAACGCTTATCCCGGCAACAACACCATCGTCGTCACCGACTATGCCGAGAACCTGACG
CGGGTCGCACAACTGATTCAGGGCATCGACACGCCGAGCGCCATCGACACTGACGTGGTGCAGATCCAGAACGGCATCGC
CGCCGACATCGCGCCAATGGTCGCCGACCTGCTCGACGCACCGGGCAATGACCCGACGCAGAAAATCGCAGTGATCGGCG
ACCCGCGCTCCAACACCATCATCATCCGCGCCGGCAGCCCCGAGCGCACGGAACTGGCGCGCAACCTGATCTACAAACTC
GACAACGCGCAGAGCAATCCGAGCAATCTGCACGTGGTGTATCTGCGCAACGCCCAGGCCGGCAAACTGGCCCAGGCCCT
GCGCGGGTTGTTGACCGGCGAGAGCGACAGCGGCACCAGCGACAATGCGCGCTCGGTGCTCAGCAACATGGGCAGTTCCA
CCAACTCGGGCGGTGGGCAAAACGGCCAGAGCGGTGCGCAAAGCAACGGCAGCACCTCGACCACCAACAACGGCAGCACC
GGCGGCAGTTACGCCCAGGGCAGCAGCGCCAGCAGCGGTGGCAGCAATGCGCAAAGCAGCGAACAGAACGTGGCCTTCAG
CGCTGGCGGCGTGACCATTCAGGCGGATGCCACCACCAACACCTTGCTGATTTCCGCGCCGGAACCGCTGTATCGCAACC
TGCGCGAAGTGATTGACCTGCTCGATCAGCGCCGCGCACAAGTGGTGATCGAAAGCCTGATCGTCGAAGTTGGCGAGGAC
GATGCCAGCGAATTCGGCGTGCAATGGCAGACCGGCAATCTCGGCGGTAAAGGCGTGATCGGCGGCGCCAACCTCGGTGG
TTCGGGGATCAACGTCAACGGCAAGACCAGCCTCGATGTACTGCCGCAAGGTTTGAACCTCGGCTACGTCAACGGCACCG
TCGACATCCCCGGCATCGGCAAGATCCTCGACCTTAAAGTGCTGGCCCGGGCGCTGAAGAGCAAGGGCGGCACCAACGTG
CTGTCGACGCCGAACCTGCTGACCCTGGACAACGAAGCCGCGAGTATTTTCGTCGGCCAGACCATCCCGTTTGTCAGCGG
CAGTTACGTCACCGGCGGCGGGGGCACCAGCAACAACCCGTTCCAGACCGTGACCCGTGAAGAGGTCGGTTTGAAGCTCA
ACGTGCGGCCGCAGATTTCCGAGGGCGGCACGGTGAAGCTCGACATCTATCAGGAAGTCAGCAGCATCGACGAACGCGCC
TCGGCGGCGGCCAATTCGGCGGGGATCGTCACCAGCAAACGCGCGCTCGACACCAGCATCCTGTTGGATGACGGGCAGAT
CATGGTGCTCGGCGGTCTGCTGCAGGATGGCTACAGCCAGAGCAATGACGCGGTGCCGTGGCTCTCGAACATTCCGGGGA
TTGGCGCACTGTTTCGCAACGAACGCCGCTCGATCACCAAGACCAATCTGATGGTGTTCCTGCGCCCGTACATCATCCGC
GACAGCGCGGCGGGCCGGAGTATTACGCTGAACCGCTACGACTTCATGCGCCGCGCTCAGGGCGGTCTGCAACCGGAGCG
CAGCTGGGCGATGCCGGACATGCAGGCGCCGCAATTGCCGGCCACTGCCCAAGGCGTGCCGGCACCGGTGTCCGGCCCGC
GTGCGACCATCAAAGCGGTGCCGCTGCAATGA

Upstream 100 bases:

>100_bases
CTCGTCTGCAACGCGCAGACGACGCCGCGACCGATGACACCGCCGGCACTCTGTCGGGCACCGTACGCATGGATCAGGCG
CTGGGCGCTAAGGAAGCTTC

Downstream 100 bases:

>100_bases
GCGCATTGCCTTACGCCTGGGCCAAGGCGCAGCGGATTCTGCTGCGCGACGGCGTGCTGACGGTGTGCCCGTCGACGCCG
GGCTGGTCGATCAGCGAGGT

Product: type II and III secretion system protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 783; Mature: 783

Protein sequence:

>783_residues
MKGSGSKRMALPMLLMALSACSNTTPPNQPPLLVDSELGRPLANTQRSGDAVLDRERAQAQARPAPKQLHNITERARSGG
SARGTTLPANPLGDQPVTLNFVDADIQAVVRALSRSTGQQFLVDPRVKGNLTLVSEGQVPAHQAYDMLLAALRMQGFSVV
DVGGVAQVVPEADAKLLGGPIYSADKPAGNGMLTRTFRLQYENAVNLIPVLRPIVSPNNPINAYPGNNTIVVTDYAENLT
RVAQLIQGIDTPSAIDTDVVQIQNGIAADIAPMVADLLDAPGNDPTQKIAVIGDPRSNTIIIRAGSPERTELARNLIYKL
DNAQSNPSNLHVVYLRNAQAGKLAQALRGLLTGESDSGTSDNARSVLSNMGSSTNSGGGQNGQSGAQSNGSTSTTNNGST
GGSYAQGSSASSGGSNAQSSEQNVAFSAGGVTIQADATTNTLLISAPEPLYRNLREVIDLLDQRRAQVVIESLIVEVGED
DASEFGVQWQTGNLGGKGVIGGANLGGSGINVNGKTSLDVLPQGLNLGYVNGTVDIPGIGKILDLKVLARALKSKGGTNV
LSTPNLLTLDNEAASIFVGQTIPFVSGSYVTGGGGTSNNPFQTVTREEVGLKLNVRPQISEGGTVKLDIYQEVSSIDERA
SAAANSAGIVTSKRALDTSILLDDGQIMVLGGLLQDGYSQSNDAVPWLSNIPGIGALFRNERRSITKTNLMVFLRPYIIR
DSAAGRSITLNRYDFMRRAQGGLQPERSWAMPDMQAPQLPATAQGVPAPVSGPRATIKAVPLQ

Sequences:

>Translated_783_residues
MKGSGSKRMALPMLLMALSACSNTTPPNQPPLLVDSELGRPLANTQRSGDAVLDRERAQAQARPAPKQLHNITERARSGG
SARGTTLPANPLGDQPVTLNFVDADIQAVVRALSRSTGQQFLVDPRVKGNLTLVSEGQVPAHQAYDMLLAALRMQGFSVV
DVGGVAQVVPEADAKLLGGPIYSADKPAGNGMLTRTFRLQYENAVNLIPVLRPIVSPNNPINAYPGNNTIVVTDYAENLT
RVAQLIQGIDTPSAIDTDVVQIQNGIAADIAPMVADLLDAPGNDPTQKIAVIGDPRSNTIIIRAGSPERTELARNLIYKL
DNAQSNPSNLHVVYLRNAQAGKLAQALRGLLTGESDSGTSDNARSVLSNMGSSTNSGGGQNGQSGAQSNGSTSTTNNGST
GGSYAQGSSASSGGSNAQSSEQNVAFSAGGVTIQADATTNTLLISAPEPLYRNLREVIDLLDQRRAQVVIESLIVEVGED
DASEFGVQWQTGNLGGKGVIGGANLGGSGINVNGKTSLDVLPQGLNLGYVNGTVDIPGIGKILDLKVLARALKSKGGTNV
LSTPNLLTLDNEAASIFVGQTIPFVSGSYVTGGGGTSNNPFQTVTREEVGLKLNVRPQISEGGTVKLDIYQEVSSIDERA
SAAANSAGIVTSKRALDTSILLDDGQIMVLGGLLQDGYSQSNDAVPWLSNIPGIGALFRNERRSITKTNLMVFLRPYIIR
DSAAGRSITLNRYDFMRRAQGGLQPERSWAMPDMQAPQLPATAQGVPAPVSGPRATIKAVPLQ
>Mature_783_residues
MKGSGSKRMALPMLLMALSACSNTTPPNQPPLLVDSELGRPLANTQRSGDAVLDRERAQAQARPAPKQLHNITERARSGG
SARGTTLPANPLGDQPVTLNFVDADIQAVVRALSRSTGQQFLVDPRVKGNLTLVSEGQVPAHQAYDMLLAALRMQGFSVV
DVGGVAQVVPEADAKLLGGPIYSADKPAGNGMLTRTFRLQYENAVNLIPVLRPIVSPNNPINAYPGNNTIVVTDYAENLT
RVAQLIQGIDTPSAIDTDVVQIQNGIAADIAPMVADLLDAPGNDPTQKIAVIGDPRSNTIIIRAGSPERTELARNLIYKL
DNAQSNPSNLHVVYLRNAQAGKLAQALRGLLTGESDSGTSDNARSVLSNMGSSTNSGGGQNGQSGAQSNGSTSTTNNGST
GGSYAQGSSASSGGSNAQSSEQNVAFSAGGVTIQADATTNTLLISAPEPLYRNLREVIDLLDQRRAQVVIESLIVEVGED
DASEFGVQWQTGNLGGKGVIGGANLGGSGINVNGKTSLDVLPQGLNLGYVNGTVDIPGIGKILDLKVLARALKSKGGTNV
LSTPNLLTLDNEAASIFVGQTIPFVSGSYVTGGGGTSNNPFQTVTREEVGLKLNVRPQISEGGTVKLDIYQEVSSIDERA
SAAANSAGIVTSKRALDTSILLDDGQIMVLGGLLQDGYSQSNDAVPWLSNIPGIGALFRNERRSITKTNLMVFLRPYIIR
DSAAGRSITLNRYDFMRRAQGGLQPERSWAMPDMQAPQLPATAQGVPAPVSGPRATIKAVPLQ

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=662, Percent_Identity=29.7583081570997, Blast_Score=245, Evalue=7e-66,
Organism=Escherichia coli, GI1789793, Length=358, Percent_Identity=25.1396648044693, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 81925; Mature: 81925

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGSGSKRMALPMLLMALSACSNTTPPNQPPLLVDSELGRPLANTQRSGDAVLDRERAQA
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHCCCCHHCCCCCCCHHHHHHHHHH
QARPAPKQLHNITERARSGGSARGTTLPANPLGDQPVTLNFVDADIQAVVRALSRSTGQQ
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCE
FLVDPRVKGNLTLVSEGQVPAHQAYDMLLAALRMQGFSVVDVGGVAQVVPEADAKLLGGP
EEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCHHHHCCC
IYSADKPAGNGMLTRTFRLQYENAVNLIPVLRPIVSPNNPINAYPGNNTIVVTDYAENLT
CCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHH
RVAQLIQGIDTPSAIDTDVVQIQNGIAADIAPMVADLLDAPGNDPTQKIAVIGDPRSNTI
HHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEE
IIRAGSPERTELARNLIYKLDNAQSNPSNLHVVYLRNAQAGKLAQALRGLLTGESDSGTS
EEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
DNARSVLSNMGSSTNSGGGQNGQSGAQSNGSTSTTNNGSTGGSYAQGSSASSGGSNAQSS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EQNVAFSAGGVTIQADATTNTLLISAPEPLYRNLREVIDLLDQRRAQVVIESLIVEVGED
CCCEEEEECCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DASEFGVQWQTGNLGGKGVIGGANLGGSGINVNGKTSLDVLPQGLNLGYVNGTVDIPGIG
CHHHCCCEEECCCCCCCEEEECCCCCCCCCCCCCCCCCEECCCCCEEEEECCEEECCCCC
KILDLKVLARALKSKGGTNVLSTPNLLTLDNEAASIFVGQTIPFVSGSYVTGGGGTSNNP
HHHHHHHHHHHHHCCCCCCEECCCCEEEECCCCCEEEECCCCCCCCCCEEECCCCCCCCC
FQTVTREEVGLKLNVRPQISEGGTVKLDIYQEVSSIDERASAAANSAGIVTSKRALDTSI
CHHEEHHHCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEECHHCCEEE
LLDDGQIMVLGGLLQDGYSQSNDAVPWLSNIPGIGALFRNERRSITKTNLMVFLRPYIIR
EEECCCEEEEECHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHEEEEEECEEEE
DSAAGRSITLNRYDFMRRAQGGLQPERSWAMPDMQAPQLPATAQGVPAPVSGPRATIKAV
CCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
PLQ
CCC
>Mature Secondary Structure
MKGSGSKRMALPMLLMALSACSNTTPPNQPPLLVDSELGRPLANTQRSGDAVLDRERAQA
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHCCCCHHCCCCCCCHHHHHHHHHH
QARPAPKQLHNITERARSGGSARGTTLPANPLGDQPVTLNFVDADIQAVVRALSRSTGQQ
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCE
FLVDPRVKGNLTLVSEGQVPAHQAYDMLLAALRMQGFSVVDVGGVAQVVPEADAKLLGGP
EEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCHHHHCCC
IYSADKPAGNGMLTRTFRLQYENAVNLIPVLRPIVSPNNPINAYPGNNTIVVTDYAENLT
CCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHH
RVAQLIQGIDTPSAIDTDVVQIQNGIAADIAPMVADLLDAPGNDPTQKIAVIGDPRSNTI
HHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEE
IIRAGSPERTELARNLIYKLDNAQSNPSNLHVVYLRNAQAGKLAQALRGLLTGESDSGTS
EEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
DNARSVLSNMGSSTNSGGGQNGQSGAQSNGSTSTTNNGSTGGSYAQGSSASSGGSNAQSS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EQNVAFSAGGVTIQADATTNTLLISAPEPLYRNLREVIDLLDQRRAQVVIESLIVEVGED
CCCEEEEECCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DASEFGVQWQTGNLGGKGVIGGANLGGSGINVNGKTSLDVLPQGLNLGYVNGTVDIPGIG
CHHHCCCEEECCCCCCCEEEECCCCCCCCCCCCCCCCCEECCCCCEEEEECCEEECCCCC
KILDLKVLARALKSKGGTNVLSTPNLLTLDNEAASIFVGQTIPFVSGSYVTGGGGTSNNP
HHHHHHHHHHHHHCCCCCCEECCCCEEEECCCCCEEEECCCCCCCCCCEEECCCCCCCCC
FQTVTREEVGLKLNVRPQISEGGTVKLDIYQEVSSIDERASAAANSAGIVTSKRALDTSI
CHHEEHHHCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEECHHCCEEE
LLDDGQIMVLGGLLQDGYSQSNDAVPWLSNIPGIGALFRNERRSITKTNLMVFLRPYIIR
EEECCCEEEEECHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHEEEEEECEEEE
DSAAGRSITLNRYDFMRRAQGGLQPERSWAMPDMQAPQLPATAQGVPAPVSGPRATIKAV
CCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
PLQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]