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The map label for this gene is sdhA [C]

Identifier: 77459385

GI number: 77459385

Start: 3631088

End: 3632818

Strand: Reverse

Name: sdhA [C]

Synonym: Pfl01_3163

Alternate gene names: 77459385

Gene position: 3632818-3631088 (Counterclockwise)

Preceding gene: 77459386

Following gene: 77459384

Centisome position: 56.42

GC content: 61.41

Gene sequence:

>1731_bases
ATGACCCGCAACACCCTCGAACAGGAATACGACATCGTCGTGATCGGCGGCGGCACTGCCGGCCCGATGGCCGCGATCAA
GGCCAAGGAAAAGAACAAGGAACTGCGCGTGCTGCTGGTCGACAAGGCCAACGTCAAGCGCAGCGGCGCGATCAGCATGG
GCATGGACGGCCTGAACAACGCGATCATCCCCGGCCACTCGACGCCCGAGCAGTACACCAAGGAAATCACCATCGCCAAC
GACGGCATCGTCAATCAGGCAGCGGTCTATGCCTACGCCACCCACAGTTTCGAAACCATCGAGCAGCTCGACCGCTGGGG
CGTGAAGTTCGAGAAGGACGAAACCGGCGATTACGCGGTGAAGAAAGTCCACCACATGGGCGCCTACGTGCTGCCGATGC
CGGAAGGGCACGACATCAAAAAAGTCCTGTATCGCCAGTTGAAGCGCGCAAGGGTCAACATCACCAATCGCCTGGTCTGC
ACCCGTTTGCTGACTGACGAGGAGGGCGCCGTCAACGGTGTGATGGGGTTCGACTGCCGCACTGCCGACTTCCATGTGAT
CAAGGCCAAGGCAGTGATCCTCGCGTGTGGCGCTGCCGGGCGCCTCGGTCTGCCGTCCTCGGGTTATCTGATGGGCACCT
ACGAAAACCCGACCAACGCCGGCGACGGTTACGCGATGGCGTATCACGCCGGGGCCGAACTGGCGAACCTCGAGTGCTTC
CAGATCAACCCGCTGATCAAGGATTACAACGGCCCGGCCTGCGCCTACGTCACCGGCCCGCTCGGTGGCTACACCGCCAA
CAACAAGGGCGAACGCTTCATCGAGTGCGACTACTGGAGCGGGCAGATGATGTGGGAATTCCACCAGGAACTGGAAAGCG
GCAACGGTCCGGTGTTCCTCAAGCTCGATCACCTGGCCGAGGAAACCATCCAGAACATCGAGGAAATCCTCCACAGCAAC
GAACGTCCGAGTCGCGGCCAGTTCCATGCCAATCGCTGCACGGATTACCGCACGCAGATGGTCGAGATGCACATCTCGGA
AATCGGTTTTTGCAGCGGCCACTCGGCGTCCGGCGTGTGGGTCAACGAGCGCGCCGAAACCTCGGTGAAAGGTTTGTACT
CGGCCGGTGACATGGCCGCCGTGCCGCACAACTACATGCTTGGCGCGTTCACCTACGGCTGGTTCGCGGGCAACAACGCG
GCGGAGTTTGTTGCCGGGCGCGAGTTCTCCGTGCTGGATGCCGAGCAGATCGAAAAGGAAAAGGCCCGGGTCTATGCACC
GCTGGATCGCGAACACGGTCTGCCGCCAGCCCAGGTGGAGTACAAGCTGCGGCGCTTCGTCAACGACTACCTGCAACCGC
CGAAAGTGACCAAGAAAATGGAAATCGGCCTGCAACGCTTCAGCGACATCGAGCGCGATCTCAACGAGATGAAGGCCAAC
AACGCCCACGAACTGATGCGCGCCATGGAAACCAGCGTGATTCGCGACTGCGCCGAAATGGCCGCCCGCGCTTCGCTGTT
CCGCGCCGAAAGTCGCTGGGGCCTGTACCACTACCGCGTCGATCATCCGCAACGGGACGACCGCGAATGGTTCTGCCACT
GCCACCTGAAGAAGGGCGAAGACGGGCGCATGACCAGTTTCAAGAAAGCCGTCGAGCCTTACATCATCCCCCTCGATGCC
GAGGAAATGCAGGCCTACGACCGCCTCCGGGTCGGCGCATTCGCCGCTTGA

Upstream 100 bases:

>100_bases
ACGACGCTCACGGCCAGCCGCTGGACTTCGAACACCTTTACTACCGTGGCGATGCGTTCCAGTACCGCCTGCGGATCGAC
CGGCAAAAAGGGGCGTGACC

Downstream 100 bases:

>100_bases
GGCATCACTAGAGAGAGTCCGAACCATGGCCTACCAAGCCCAGGAAATCTTCTTCCGCTCCAACGCCCCCGTCACCGTGG
ACGAGGACAAATGCATCGCC

Product: putative oxidoreductase

Products: FADH2; Fumarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MTRNTLEQEYDIVVIGGGTAGPMAAIKAKEKNKELRVLLVDKANVKRSGAISMGMDGLNNAIIPGHSTPEQYTKEITIAN
DGIVNQAAVYAYATHSFETIEQLDRWGVKFEKDETGDYAVKKVHHMGAYVLPMPEGHDIKKVLYRQLKRARVNITNRLVC
TRLLTDEEGAVNGVMGFDCRTADFHVIKAKAVILACGAAGRLGLPSSGYLMGTYENPTNAGDGYAMAYHAGAELANLECF
QINPLIKDYNGPACAYVTGPLGGYTANNKGERFIECDYWSGQMMWEFHQELESGNGPVFLKLDHLAEETIQNIEEILHSN
ERPSRGQFHANRCTDYRTQMVEMHISEIGFCSGHSASGVWVNERAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGNNA
AEFVAGREFSVLDAEQIEKEKARVYAPLDREHGLPPAQVEYKLRRFVNDYLQPPKVTKKMEIGLQRFSDIERDLNEMKAN
NAHELMRAMETSVIRDCAEMAARASLFRAESRWGLYHYRVDHPQRDDREWFCHCHLKKGEDGRMTSFKKAVEPYIIPLDA
EEMQAYDRLRVGAFAA

Sequences:

>Translated_576_residues
MTRNTLEQEYDIVVIGGGTAGPMAAIKAKEKNKELRVLLVDKANVKRSGAISMGMDGLNNAIIPGHSTPEQYTKEITIAN
DGIVNQAAVYAYATHSFETIEQLDRWGVKFEKDETGDYAVKKVHHMGAYVLPMPEGHDIKKVLYRQLKRARVNITNRLVC
TRLLTDEEGAVNGVMGFDCRTADFHVIKAKAVILACGAAGRLGLPSSGYLMGTYENPTNAGDGYAMAYHAGAELANLECF
QINPLIKDYNGPACAYVTGPLGGYTANNKGERFIECDYWSGQMMWEFHQELESGNGPVFLKLDHLAEETIQNIEEILHSN
ERPSRGQFHANRCTDYRTQMVEMHISEIGFCSGHSASGVWVNERAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGNNA
AEFVAGREFSVLDAEQIEKEKARVYAPLDREHGLPPAQVEYKLRRFVNDYLQPPKVTKKMEIGLQRFSDIERDLNEMKAN
NAHELMRAMETSVIRDCAEMAARASLFRAESRWGLYHYRVDHPQRDDREWFCHCHLKKGEDGRMTSFKKAVEPYIIPLDA
EEMQAYDRLRVGAFAA
>Mature_575_residues
TRNTLEQEYDIVVIGGGTAGPMAAIKAKEKNKELRVLLVDKANVKRSGAISMGMDGLNNAIIPGHSTPEQYTKEITIAND
GIVNQAAVYAYATHSFETIEQLDRWGVKFEKDETGDYAVKKVHHMGAYVLPMPEGHDIKKVLYRQLKRARVNITNRLVCT
RLLTDEEGAVNGVMGFDCRTADFHVIKAKAVILACGAAGRLGLPSSGYLMGTYENPTNAGDGYAMAYHAGAELANLECFQ
INPLIKDYNGPACAYVTGPLGGYTANNKGERFIECDYWSGQMMWEFHQELESGNGPVFLKLDHLAEETIQNIEEILHSNE
RPSRGQFHANRCTDYRTQMVEMHISEIGFCSGHSASGVWVNERAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGNNAA
EFVAGREFSVLDAEQIEKEKARVYAPLDREHGLPPAQVEYKLRRFVNDYLQPPKVTKKMEIGLQRFSDIERDLNEMKANN
AHELMRAMETSVIRDCAEMAARASLFRAESRWGLYHYRVDHPQRDDREWFCHCHLKKGEDGRMTSFKKAVEPYIIPLDAE
EMQAYDRLRVGAFAA

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=606, Percent_Identity=24.7524752475248, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1790597, Length=585, Percent_Identity=20.3418803418803, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322701, Length=572, Percent_Identity=22.7272727272727, Blast_Score=80, Evalue=9e-16,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1 [C]

Molecular weight: Translated: 64585; Mature: 64454

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNTLEQEYDIVVIGGGTAGPMAAIKAKEKNKELRVLLVDKANVKRSGAISMGMDGLNN
CCCCCCCCCCCEEEECCCCCCCHHHEEECCCCCCEEEEEEECCCCCCCCCEEECCCCCCC
AIIPGHSTPEQYTKEITIANDGIVNQAAVYAYATHSFETIEQLDRWGVKFEKDETGDYAV
CCCCCCCCHHHHHCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCEEECCCCCCHHH
KKVHHMGAYVLPMPEGHDIKKVLYRQLKRARVNITNRLVCTRLLTDEEGAVNGVMGFDCR
HHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCEEECCCC
TADFHVIKAKAVILACGAAGRLGLPSSGYLMGTYENPTNAGDGYAMAYHAGAELANLECF
CCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEE
QINPLIKDYNGPACAYVTGPLGGYTANNKGERFIECDYWSGQMMWEFHQELESGNGPVFL
EECCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEE
KLDHLAEETIQNIEEILHSNERPSRGQFHANRCTDYRTQMVEMHISEIGFCSGHSASGVW
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
VNERAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGNNAAEFVAGREFSVLDAEQIEKE
ECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHCCCCEEECCHHHHHHH
KARVYAPLDREHGLPPAQVEYKLRRFVNDYLQPPKVTKKMEIGLQRFSDIERDLNEMKAN
HHEEECCCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
NAHELMRAMETSVIRDCAEMAARASLFRAESRWGLYHYRVDHPQRDDREWFCHCHLKKGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEECCC
DGRMTSFKKAVEPYIIPLDAEEMQAYDRLRVGAFAA
CCCHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRNTLEQEYDIVVIGGGTAGPMAAIKAKEKNKELRVLLVDKANVKRSGAISMGMDGLNN
CCCCCCCCCCEEEECCCCCCCHHHEEECCCCCCEEEEEEECCCCCCCCCEEECCCCCCC
AIIPGHSTPEQYTKEITIANDGIVNQAAVYAYATHSFETIEQLDRWGVKFEKDETGDYAV
CCCCCCCCHHHHHCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCEEECCCCCCHHH
KKVHHMGAYVLPMPEGHDIKKVLYRQLKRARVNITNRLVCTRLLTDEEGAVNGVMGFDCR
HHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCEEECCCC
TADFHVIKAKAVILACGAAGRLGLPSSGYLMGTYENPTNAGDGYAMAYHAGAELANLECF
CCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEE
QINPLIKDYNGPACAYVTGPLGGYTANNKGERFIECDYWSGQMMWEFHQELESGNGPVFL
EECCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEE
KLDHLAEETIQNIEEILHSNERPSRGQFHANRCTDYRTQMVEMHISEIGFCSGHSASGVW
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
VNERAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGNNAAEFVAGREFSVLDAEQIEKE
ECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHCCCCEEECCHHHHHHH
KARVYAPLDREHGLPPAQVEYKLRRFVNDYLQPPKVTKKMEIGLQRFSDIERDLNEMKAN
HHEEECCCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
NAHELMRAMETSVIRDCAEMAARASLFRAESRWGLYHYRVDHPQRDDREWFCHCHLKKGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEECCC
DGRMTSFKKAVEPYIIPLDAEEMQAYDRLRVGAFAA
CCCHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]

Substrates: FAD; Succinate [C]

Specific reaction: FAD + Succinate --> FADH2 + Fumarate [C]

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]