Click here to switch to the map view.

The map label for this gene is lexA2 [H]

Identifier: 77459374

GI number: 77459374

Start: 3620095

End: 3620712

Strand: Reverse

Name: lexA2 [H]

Synonym: Pfl01_3152

Alternate gene names: 77459374

Gene position: 3620712-3620095 (Counterclockwise)

Preceding gene: 77459375

Following gene: 77459373

Centisome position: 56.24

GC content: 65.7

Gene sequence:

>618_bases
ATGTACTCCATGACGACTCTGACTCCCCGCCGTACCGCCATCCTGACCTTTATCCGCGAACGCATCGCCGAACACGGTCA
GCCCCCAAGCCTCGCTGAAATCAGCGAGGCTTTTGGTTTTGCCTCCCGCAGCGTGGCGCGCAAGCATGTGCTGGCGCTCA
CCGAAGCCGGGTTTATCGAGGTCAACCCGCATCAGGCCCGGGGCATTCGCCTGCTCGGGCAGCCGGCGCGGCCGGAACTG
CTCGAAGTACCGGTGCTCGGCCGGGTGGCCGCTGGTGCGCCCATCGGTGCCGATGCCGACCTGCACAATCGGCTGATGCT
CGATCCCGCGCTGTTCTCCCGCACGCCCGACTACATGCTGCGGGTGCAGGGCGATTCGATGATCGAGGACGGCATTCTCG
ACGGCGACCTGGTCGGCGTGCGACGCAACCCCGAAGCGCTCAATGGCCAGATCGTGGTGGCGCGACTCGACGGTGAGGTC
ACGATCAAACGCTTCGAGCGGGTCGGCGATGCGGTTCGCCTGTTACCGCGCAACCCGGCGTACCAGCCGATTGTGGTGCG
GGCCGATCAGGACCTGGCCATCGAAGGGGTGTTCTGCGGTCTGGTGAGGCAAGGCTGA

Upstream 100 bases:

>100_bases
CCAAGGTTGTGGCGTTCATTGATTACATCGCGGGGTTTCTCGCGGAGCGCGGTTTGAGCTGACTGGCGGTGCTTGTCAGG
AAAATCCCACAAGAGTACAA

Downstream 100 bases:

>100_bases
TGGGTGCCGTCGTTGCACTGGATACGCTGTTCAATGGCGGCCAGGTCTGGAAGGGCCGGCCTGCGCCACCGGCCGCCAGC
CCGCAACCCACCGGGCACGC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MYSMTTLTPRRTAILTFIRERIAEHGQPPSLAEISEAFGFASRSVARKHVLALTEAGFIEVNPHQARGIRLLGQPARPEL
LEVPVLGRVAAGAPIGADADLHNRLMLDPALFSRTPDYMLRVQGDSMIEDGILDGDLVGVRRNPEALNGQIVVARLDGEV
TIKRFERVGDAVRLLPRNPAYQPIVVRADQDLAIEGVFCGLVRQG

Sequences:

>Translated_205_residues
MYSMTTLTPRRTAILTFIRERIAEHGQPPSLAEISEAFGFASRSVARKHVLALTEAGFIEVNPHQARGIRLLGQPARPEL
LEVPVLGRVAAGAPIGADADLHNRLMLDPALFSRTPDYMLRVQGDSMIEDGILDGDLVGVRRNPEALNGQIVVARLDGEV
TIKRFERVGDAVRLLPRNPAYQPIVVRADQDLAIEGVFCGLVRQG
>Mature_205_residues
MYSMTTLTPRRTAILTFIRERIAEHGQPPSLAEISEAFGFASRSVARKHVLALTEAGFIEVNPHQARGIRLLGQPARPEL
LEVPVLGRVAAGAPIGADADLHNRLMLDPALFSRTPDYMLRVQGDSMIEDGILDGDLVGVRRNPEALNGQIVVARLDGEV
TIKRFERVGDAVRLLPRNPAYQPIVVRADQDLAIEGVFCGLVRQG

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=203, Percent_Identity=42.8571428571429, Blast_Score=163, Evalue=8e-42,
Organism=Escherichia coli, GI1787431, Length=127, Percent_Identity=34.6456692913386, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 22420; Mature: 22420

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSMTTLTPRRTAILTFIRERIAEHGQPPSLAEISEAFGFASRSVARKHVLALTEAGFIE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHEEECCCEEE
VNPHQARGIRLLGQPARPELLEVPVLGRVAAGAPIGADADLHNRLMLDPALFSRTPDYML
ECCCCCCCEEEECCCCCCCEEECCCCCHHHCCCCCCCCCCCCCEEEECHHHHCCCCCCEE
RVQGDSMIEDGILDGDLVGVRRNPEALNGQIVVARLDGEVTIKRFERVGDAVRLLPRNPA
EECCCCHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEECCCCCC
YQPIVVRADQDLAIEGVFCGLVRQG
CCCEEEECCCCEEEEHEEEEEECCC
>Mature Secondary Structure
MYSMTTLTPRRTAILTFIRERIAEHGQPPSLAEISEAFGFASRSVARKHVLALTEAGFIE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHEEECCCEEE
VNPHQARGIRLLGQPARPELLEVPVLGRVAAGAPIGADADLHNRLMLDPALFSRTPDYML
ECCCCCCCEEEECCCCCCCEEECCCCCHHHCCCCCCCCCCCCCEEEECHHHHCCCCCCEE
RVQGDSMIEDGILDGDLVGVRRNPEALNGQIVVARLDGEVTIKRFERVGDAVRLLPRNPA
EECCCCHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEECCCCCC
YQPIVVRADQDLAIEGVFCGLVRQG
CCCEEEECCCCEEEEHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]