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The map label for this gene is pilT [H]

Identifier: 77459304

GI number: 77459304

Start: 3530653

End: 3531765

Strand: Reverse

Name: pilT [H]

Synonym: Pfl01_3082

Alternate gene names: 77459304

Gene position: 3531765-3530653 (Counterclockwise)

Preceding gene: 77459307

Following gene: 77459303

Centisome position: 54.85

GC content: 56.51

Gene sequence:

>1113_bases
ATGGAAATCGACGCATTGCTCAAACAACTGGCGAGCCGCCACGGTTCGGATCTGTTCCTGTCCACGGGCGCACCGCCGAG
TGCGCGGTTCGACGGGGTGCTCACGCCCTTGAACGATCAGCCCTTCAAGCCCGGCGAAGTGGCGACTGTCGCCACCTCCC
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCCCGCACCGGCATCGGCCGC
TTCCGGGTCAATATATTCAAACAGCGCAACGATGTGTCAATCGTGATCCGTAACGTCAAACTCGACATTCCGCGATTCGA
AGACCTCAAATTGCCAGCGGTATTGCTGGAAACGGTGATGCTCAAACAGGGGCTGATCCTGTTTGTCGGTGCTACGGACT
CGGGCAAGTCGACCTCGCTGGCGGCATTGATCGATCATCGCAATCGCCATAGCAGCGGGCACATCATCACCATTGAAGAC
CCGGTGGAATACATCCATCGCCACAAGAAATCGATCATCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTTCACGC
TGCACTGAAAAACACCCTGCGCCAGGCCCCGGACGTGGTGCTGATCGGCGAGATCCGTGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCGCCGAAACCGGGCATCTGGTGCTGTCGACATTGCACGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAATTTCTTTCCCGAAGAGCGTCGCCCGCAATTGCTGCACAGTTTAGGCAATCATTTGAAGGCCTTCGTTTCCCAACG
TCTGGTACGCACGCTTGATGGCCAGCGACGGGCGGCAGTGGAAGTCATGTTGGGGTCGCCCACCATTGCCGACCTCATTC
GTCGCAATGAACTGGGCGAGTTGAAAGTCATCATGGAAAAATCCACAGAGCTGGGCATGCAGACTTTCGATGACGCACTT
TACAAGTTGGTGGCTGAAGGTGTTTTAAGTGTGCAGGAGGCACTTAAACATGCGGACTCAATCAATAATTTGAAGTTGAA
GTTGAAGTTGAAGTTGGAGCGCCAAGGCGAGACGCCCACTTCAAGTTCGGGTGAATGGGGGCTGGCTGATTGA

Upstream 100 bases:

>100_bases
AGGCGGCGTCTATTATGCTAGAGCGGTAGACGCTGTAAGGCCCGTCGCGTCACGACGCACGGTCTTAAGTCCATTGAAAG
CTTCGCAGGTGCTTTTGGCC

Downstream 100 bases:

>100_bases
AGGGGTTGATTTCAGCCTCGGTTTGTTTGATTGAAATGTTGTCTCATGAATAACGACTGGAAAGTTTGTTGGGTGGAAGT
AGGATCGCCTCCGCCAAGCA

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MEIDALLKQLASRHGSDLFLSTGAPPSARFDGVLTPLNDQPFKPGEVATVATSLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPAVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
INFFPEERRPQLLHSLGNHLKAFVSQRLVRTLDGQRRAAVEVMLGSPTIADLIRRNELGELKVIMEKSTELGMQTFDDAL
YKLVAEGVLSVQEALKHADSINNLKLKLKLKLERQGETPTSSSGEWGLAD

Sequences:

>Translated_370_residues
MEIDALLKQLASRHGSDLFLSTGAPPSARFDGVLTPLNDQPFKPGEVATVATSLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPAVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
INFFPEERRPQLLHSLGNHLKAFVSQRLVRTLDGQRRAAVEVMLGSPTIADLIRRNELGELKVIMEKSTELGMQTFDDAL
YKLVAEGVLSVQEALKHADSINNLKLKLKLKLERQGETPTSSSGEWGLAD
>Mature_370_residues
MEIDALLKQLASRHGSDLFLSTGAPPSARFDGVLTPLNDQPFKPGEVATVATSLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPAVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
INFFPEERRPQLLHSLGNHLKAFVSQRLVRTLDGQRRAAVEVMLGSPTIADLIRRNELGELKVIMEKSTELGMQTFDDAL
YKLVAEGVLSVQEALKHADSINNLKLKLKLKLERQGETPTSSSGEWGLAD

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=314, Percent_Identity=36.624203821656, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789723, Length=318, Percent_Identity=32.7044025157233, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786296, Length=261, Percent_Identity=33.7164750957854, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41301; Mature: 41301

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIDALLKQLASRHGSDLFLSTGAPPSARFDGVLTPLNDQPFKPGEVATVATSLMDAEQR
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
REFDRDLEMNLAISRTGIGRFRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPAVLLETVM
HHHCCCCEEEEEEEECCCCEEEEEEEECCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHH
LKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIEDPVEYIHRHKKSIINQREVGV
HHCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
DTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
INFFPEERRPQLLHSLGNHLKAFVSQRLVRTLDGQRRAAVEVMLGSPTIADLIRRNELGE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCC
LKVIMEKSTELGMQTFDDALYKLVAEGVLSVQEALKHADSINNLKLKLKLKLERQGETPT
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCC
SSSGEWGLAD
CCCCCCCCCC
>Mature Secondary Structure
MEIDALLKQLASRHGSDLFLSTGAPPSARFDGVLTPLNDQPFKPGEVATVATSLMDAEQR
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
REFDRDLEMNLAISRTGIGRFRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPAVLLETVM
HHHCCCCEEEEEEEECCCCEEEEEEEECCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHH
LKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIEDPVEYIHRHKKSIINQREVGV
HHCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
DTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
INFFPEERRPQLLHSLGNHLKAFVSQRLVRTLDGQRRAAVEVMLGSPTIADLIRRNELGE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCC
LKVIMEKSTELGMQTFDDALYKLVAEGVLSVQEALKHADSINNLKLKLKLKLERQGETPT
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCC
SSSGEWGLAD
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]