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The map label for this gene is acsA1 [H]

Identifier: 77459302

GI number: 77459302

Start: 3524719

End: 3526674

Strand: Direct

Name: acsA1 [H]

Synonym: Pfl01_3080

Alternate gene names: 77459302

Gene position: 3524719-3526674 (Clockwise)

Preceding gene: 77459301

Following gene: 77459305

Centisome position: 54.75

GC content: 62.37

Gene sequence:

>1956_bases
ATGTCCGACATTCTCTGGCAGCCCGATGCCAAACGCATTGCCCGGTCCCGCATGGACGGCTTTCGGCGCTTCATCAATCA
GCGCCACCACCTGAAGCTCGACGACTACCCTGCGCTGCACCAATGGTCCATCGACCAGCGCGAAGCGTTCTGGCAAGCGA
TCGTGGATTTCTTCGGCATCAGTTTTCACACCCAACCGGACGCCGTTCTGCGCGGAGGCCTGAAAATGCCGGGTGCCGAG
TGGTTTCCCGGTGCCACCCTGAACTTCGCCGAGCATCTGCTTAGCCGACGCGACGATGCCATCGCCGTGATCGCTATCGG
GGAAAACGGCCAGCGTGAACTGCTGACCTGGGCCGAGCTGGCCCAGCAAGTCGCCGGGTTCCAGGCCAGCCTGCAAGCGG
CCGGCGTGTTGGTTGGCGACCGGATTGCGGCCTGCATGCCCAACACCTGGCAAACCCTGGTGGCGATGCTCGCGACCACC
AGTCTCGGGGCAATCTGGTCATGCTCCTCGCCGGACTTCGGCACCCACGGCGTGATCGACCGCTTCGGCCAGATCGAACC
GAAGGTGCTGATCACCTGCGCCGGCTACCGTTACGCTGGCAAGGAGATCGACCAGACCGTCAAGGTCAATGAAATCCTCG
AACAGCTGCCGTCACTGCAACAGCTGATCATCGTGCCCTACGCGCGACCTCAAGCCCATGCCACGGATTACCGGACACCC
GCCAACGTCACGCTGTGGGACGACTTCTACGAACCGGGCGATGAGCCGCAATTCGTCCCGGTGCCGTTCGATCATCCGCT
GTACGTGCTTTATTCCAGCGGCACCACCGGCGTGCCGAAGTGCATCGTGCACAGCACCGGCGGCGTGCTGCTGCAACACG
TCAAGGAGCACGGGCTGCATGTTGACCTCGGACCCGGCGACCGTTTGTTCTACTACACCACCTGCGGCTGGATGATGTGG
AACTGGCTGGTCTCGGCACTGGCGGTCGGCAGCGCGGTGGTGCTGTATGACGGTTCACCGTTTTATCCGGACAACGAGCG
CTTGCTGGAGCTGCTGGACGACGAACAGGTCAGCGTGTTCGGCACCAGCCCGAAATTCCTCGCGACCCTGGAAAGCAGCG
GCATCAAACCGCGCGAAAGCTACGACCTGAGCCACCTGAGAACCCTGCTTTGCACCGGCTCCGCACTGTCACCGCAGAGT
TATGACTTCGTCTATCGCGACTTCAAACCGGATGTGTGCTTGTCCTCGATGTCCGGCGGTACGGACATCGTGTCCTGCTT
CGTCAACGGCAACCCGATGTCAGCAGTCCGCCGGGGCGAAATCATGGGCAAGAGCCTGGCCATGGCGGTGGAAGTGTGGA
ACGACGCCGGGCAACCGGTCGTTGGCGAAAAAGGCGAACTGGTGTGTACCCGGTCATTTCCGGCGGTGCCTATCGGACTC
TGGAACGATCCCGATGGCGAGAAACTGCGCAAATCCTATTTCAGCCAGTTCCCCGGCGTCTGGGCCCAGGGTGACTACGC
CGAACAACTGCCCCACGGCGGGATGCTGATTCACGGCCGCTCCGACGCCGTGCTCAACCCCGGCGGCGTGCGCATCGGCA
CGGCGGAAATCTACCGTCAGGTGGAGAAAGTCCCGCAAGTGCTGGACAGCGTCGCCATCGGCCAACAGTGGCAGGACGAT
GTGCGCGTGGTGCTGTTCGTGCACCTCAAGGACGGCGTAACGCTGGATGACACGCTGCAACAGCAGATCCGCCAGGTGAT
CCGTGCCAACACCACACCGCGCCATGTGCCGGCGAAGATCGTCGCGGTCACCGACATTCCGCGCACCATCAGCGGCAAAG
TGGTGGAGCTGGCCGTGCGCAATGTGGTGCACGGTGAACCGGTGAAAAACACCGATGCGCTGGCCAATCCGCAAGCGCTC
GAGCAGTTTCGCGATCGACCGGAATTGAACAGCTGA

Upstream 100 bases:

>100_bases
CGCGTGGAATATCGATGGCGGGTGGCTCGCGCAGTAAGCGTGCAAATGATCGCTCCCACGCTGTCGTGGGAGCCTTCTGA
CCAACAATAAGAGGCAAACC

Downstream 100 bases:

>100_bases
CAACAGCGCTCCTGTGCCAGGAGCGCCGTTCTGTAATGCAGACGGATTATTCAGGTCGGTTCAGGGTCAGCACCACCGGG
TCGGCAATCGCACCGGTGCG

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 651; Mature: 650

Protein sequence:

>651_residues
MSDILWQPDAKRIARSRMDGFRRFINQRHHLKLDDYPALHQWSIDQREAFWQAIVDFFGISFHTQPDAVLRGGLKMPGAE
WFPGATLNFAEHLLSRRDDAIAVIAIGENGQRELLTWAELAQQVAGFQASLQAAGVLVGDRIAACMPNTWQTLVAMLATT
SLGAIWSCSSPDFGTHGVIDRFGQIEPKVLITCAGYRYAGKEIDQTVKVNEILEQLPSLQQLIIVPYARPQAHATDYRTP
ANVTLWDDFYEPGDEPQFVPVPFDHPLYVLYSSGTTGVPKCIVHSTGGVLLQHVKEHGLHVDLGPGDRLFYYTTCGWMMW
NWLVSALAVGSAVVLYDGSPFYPDNERLLELLDDEQVSVFGTSPKFLATLESSGIKPRESYDLSHLRTLLCTGSALSPQS
YDFVYRDFKPDVCLSSMSGGTDIVSCFVNGNPMSAVRRGEIMGKSLAMAVEVWNDAGQPVVGEKGELVCTRSFPAVPIGL
WNDPDGEKLRKSYFSQFPGVWAQGDYAEQLPHGGMLIHGRSDAVLNPGGVRIGTAEIYRQVEKVPQVLDSVAIGQQWQDD
VRVVLFVHLKDGVTLDDTLQQQIRQVIRANTTPRHVPAKIVAVTDIPRTISGKVVELAVRNVVHGEPVKNTDALANPQAL
EQFRDRPELNS

Sequences:

>Translated_651_residues
MSDILWQPDAKRIARSRMDGFRRFINQRHHLKLDDYPALHQWSIDQREAFWQAIVDFFGISFHTQPDAVLRGGLKMPGAE
WFPGATLNFAEHLLSRRDDAIAVIAIGENGQRELLTWAELAQQVAGFQASLQAAGVLVGDRIAACMPNTWQTLVAMLATT
SLGAIWSCSSPDFGTHGVIDRFGQIEPKVLITCAGYRYAGKEIDQTVKVNEILEQLPSLQQLIIVPYARPQAHATDYRTP
ANVTLWDDFYEPGDEPQFVPVPFDHPLYVLYSSGTTGVPKCIVHSTGGVLLQHVKEHGLHVDLGPGDRLFYYTTCGWMMW
NWLVSALAVGSAVVLYDGSPFYPDNERLLELLDDEQVSVFGTSPKFLATLESSGIKPRESYDLSHLRTLLCTGSALSPQS
YDFVYRDFKPDVCLSSMSGGTDIVSCFVNGNPMSAVRRGEIMGKSLAMAVEVWNDAGQPVVGEKGELVCTRSFPAVPIGL
WNDPDGEKLRKSYFSQFPGVWAQGDYAEQLPHGGMLIHGRSDAVLNPGGVRIGTAEIYRQVEKVPQVLDSVAIGQQWQDD
VRVVLFVHLKDGVTLDDTLQQQIRQVIRANTTPRHVPAKIVAVTDIPRTISGKVVELAVRNVVHGEPVKNTDALANPQAL
EQFRDRPELNS
>Mature_650_residues
SDILWQPDAKRIARSRMDGFRRFINQRHHLKLDDYPALHQWSIDQREAFWQAIVDFFGISFHTQPDAVLRGGLKMPGAEW
FPGATLNFAEHLLSRRDDAIAVIAIGENGQRELLTWAELAQQVAGFQASLQAAGVLVGDRIAACMPNTWQTLVAMLATTS
LGAIWSCSSPDFGTHGVIDRFGQIEPKVLITCAGYRYAGKEIDQTVKVNEILEQLPSLQQLIIVPYARPQAHATDYRTPA
NVTLWDDFYEPGDEPQFVPVPFDHPLYVLYSSGTTGVPKCIVHSTGGVLLQHVKEHGLHVDLGPGDRLFYYTTCGWMMWN
WLVSALAVGSAVVLYDGSPFYPDNERLLELLDDEQVSVFGTSPKFLATLESSGIKPRESYDLSHLRTLLCTGSALSPQSY
DFVYRDFKPDVCLSSMSGGTDIVSCFVNGNPMSAVRRGEIMGKSLAMAVEVWNDAGQPVVGEKGELVCTRSFPAVPIGLW
NDPDGEKLRKSYFSQFPGVWAQGDYAEQLPHGGMLIHGRSDAVLNPGGVRIGTAEIYRQVEKVPQVLDSVAIGQQWQDDV
RVVLFVHLKDGVTLDDTLQQQIRQVIRANTTPRHVPAKIVAVTDIPRTISGKVVELAVRNVVHGEPVKNTDALANPQALE
QFRDRPELNS

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=659, Percent_Identity=43.3990895295903, Blast_Score=569, Evalue=1e-162,
Organism=Homo sapiens, GI28416953, Length=639, Percent_Identity=25.8215962441315, Blast_Score=189, Evalue=1e-47,
Organism=Homo sapiens, GI8923896, Length=659, Percent_Identity=24.7344461305008, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI253970489, Length=672, Percent_Identity=24.2559523809524, Blast_Score=170, Evalue=5e-42,
Organism=Homo sapiens, GI13375727, Length=582, Percent_Identity=26.1168384879725, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI38505220, Length=533, Percent_Identity=23.827392120075, Blast_Score=101, Evalue=3e-21,
Organism=Homo sapiens, GI157311624, Length=535, Percent_Identity=22.6168224299065, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI157311622, Length=535, Percent_Identity=22.6168224299065, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI58082049, Length=535, Percent_Identity=22.6168224299065, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=21.3084112149533, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1790505, Length=640, Percent_Identity=25.625, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786529, Length=657, Percent_Identity=24.3531202435312, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17569507, Length=636, Percent_Identity=37.5786163522013, Blast_Score=450, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI71982997, Length=654, Percent_Identity=24.6177370030581, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71983001, Length=654, Percent_Identity=24.6177370030581, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323182, Length=649, Percent_Identity=25.57781201849, Blast_Score=190, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6319264, Length=654, Percent_Identity=25.5351681957187, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667955, Length=660, Percent_Identity=25.4545454545455, Blast_Score=208, Evalue=9e-54,
Organism=Drosophila melanogaster, GI62472339, Length=558, Percent_Identity=26.1648745519713, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24667959, Length=527, Percent_Identity=25.8064516129032, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21355225, Length=579, Percent_Identity=25.2158894645941, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 72102; Mature: 71971

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDILWQPDAKRIARSRMDGFRRFINQRHHLKLDDYPALHQWSIDQREAFWQAIVDFFGI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SFHTQPDAVLRGGLKMPGAEWFPGATLNFAEHLLSRRDDAIAVIAIGENGQRELLTWAEL
CCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
AQQVAGFQASLQAAGVLVGDRIAACMPNTWQTLVAMLATTSLGAIWSCSSPDFGTHGVID
HHHHHHHHHHHHHCCEEECCHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
RFGQIEPKVLITCAGYRYAGKEIDQTVKVNEILEQLPSLQQLIIVPYARPQAHATDYRTP
HHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCC
ANVTLWDDFYEPGDEPQFVPVPFDHPLYVLYSSGTTGVPKCIVHSTGGVLLQHVKEHGLH
CCEEEEHHHCCCCCCCCEEECCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHCCCE
VDLGPGDRLFYYTTCGWMMWNWLVSALAVGSAVVLYDGSPFYPDNERLLELLDDEQVSVF
EEECCCCEEEEEECHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCCEEEE
GTSPKFLATLESSGIKPRESYDLSHLRTLLCTGSALSPQSYDFVYRDFKPDVCLSSMSGG
ECCCHHEEEHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHCCCCC
TDIVSCFVNGNPMSAVRRGEIMGKSLAMAVEVWNDAGQPVVGEKGELVCTRSFPAVPIGL
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCEEEEC
WNDPDGEKLRKSYFSQFPGVWAQGDYAEQLPHGGMLIHGRSDAVLNPGGVRIGTAEIYRQ
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEECCCCCEECCCCCEECHHHHHHH
VEKVPQVLDSVAIGQQWQDDVRVVLFVHLKDGVTLDDTLQQQIRQVIRANTTPRHVPAKI
HHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEE
VAVTDIPRTISGKVVELAVRNVVHGEPVKNTDALANPQALEQFRDRPELNS
EEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDILWQPDAKRIARSRMDGFRRFINQRHHLKLDDYPALHQWSIDQREAFWQAIVDFFGI
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SFHTQPDAVLRGGLKMPGAEWFPGATLNFAEHLLSRRDDAIAVIAIGENGQRELLTWAEL
CCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
AQQVAGFQASLQAAGVLVGDRIAACMPNTWQTLVAMLATTSLGAIWSCSSPDFGTHGVID
HHHHHHHHHHHHHCCEEECCHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
RFGQIEPKVLITCAGYRYAGKEIDQTVKVNEILEQLPSLQQLIIVPYARPQAHATDYRTP
HHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCC
ANVTLWDDFYEPGDEPQFVPVPFDHPLYVLYSSGTTGVPKCIVHSTGGVLLQHVKEHGLH
CCEEEEHHHCCCCCCCCEEECCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHCCCE
VDLGPGDRLFYYTTCGWMMWNWLVSALAVGSAVVLYDGSPFYPDNERLLELLDDEQVSVF
EEECCCCEEEEEECHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCCEEEE
GTSPKFLATLESSGIKPRESYDLSHLRTLLCTGSALSPQSYDFVYRDFKPDVCLSSMSGG
ECCCHHEEEHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHCCCCC
TDIVSCFVNGNPMSAVRRGEIMGKSLAMAVEVWNDAGQPVVGEKGELVCTRSFPAVPIGL
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCEEEEC
WNDPDGEKLRKSYFSQFPGVWAQGDYAEQLPHGGMLIHGRSDAVLNPGGVRIGTAEIYRQ
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEECCCCCEECCCCCEECHHHHHHH
VEKVPQVLDSVAIGQQWQDDVRVVLFVHLKDGVTLDDTLQQQIRQVIRANTTPRHVPAKI
HHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEE
VAVTDIPRTISGKVVELAVRNVVHGEPVKNTDALANPQALEQFRDRPELNS
EEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]