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The map label for this gene is ada [H]

Identifier: 77459163

GI number: 77459163

Start: 3379813

End: 3380886

Strand: Direct

Name: ada [H]

Synonym: Pfl01_2938

Alternate gene names: 77459163

Gene position: 3379813-3380886 (Clockwise)

Preceding gene: 77459162

Following gene: 77459165

Centisome position: 52.49

GC content: 65.08

Gene sequence:

>1074_bases
ATGAAAACGCTTTCAACCTCGCTGAGCACTGAAGACGATCCACGCTGGGCGGCTGTCGTGGCACGCGACCCTCGGGCGGA
CGGCCAGTTTGTCTATGCCGTGAAAACCACCGGCATCTATTGCCGCCCCAGCAGTCTGGCGCGCTTGCCGAAACCGCAGA
ACGTCGAGTTCTTCGATACCGCCGAGGACGCCGAAGCCGCGGGCTATCGCCCCAGCAAACGGGCGAGCAAGGATCAGACC
GAAGTCGCAGCGCAGCACGCCGCTACCGTTGCGGCAGCCTGTCGCCAGATCGAAGCGTCGGACAGCCTGCCGGCGCTCAA
CGATCTCGCCGAGACCGCCGGCCTGAGCGCCTTCCATTTTCACCGGGTCTTCAAAGCCGCAACGGGCCTGACGCCCAAAG
GCTATGCCGCCGCGCATCGCTCGCGTCGGGTTCGCCAGCGTCTGGCGGACGGTAGCTCAGTGACGGAGGCGCTGTATGAC
GCCGGTTTCAATTCCAACAGCCGGTTCTACGAGGCGGCCGATCAGGTGCTGGGCATGAAACCCGGCGACTTCCGATCCGC
CGGGCAGAACAACGACATCCGCTTCGCCGTCGGCCAGTGCTCGCTCGGCGCCATTCTGGTGGCGCAGAGCGAGCGCGGGA
TCTGCGCGATTCTGCTGGGGGACGATCCGCACCAATTGGTGTGCGACCTGCAGGACCAGTTTCGCCGGGCCAACCTGATC
GGCGCCGACACCGAGTTCGAACAACTGATCGCGCGCGTCGTCGGCTTCATCGAAGCGCCTGCCATCGGCCTGGATTTGCC
GCTGGATGTGCGCGGCACGGCGTTTCAGGAACGGGTGTGGCAGGCACTGCGCGAAATACCGGTCGGCAGCACCGCCAGCT
ATGCGGACATCGCCTTGCGTATCGGCTCACCAAAAGCCGTACGCGCAGTGGCCCAGGCTTGCGGCGCCAACAGCCTGGCG
GTAGCGATCCCCTGTCATCGCGTTGTGCGCAGTGACGGCAATCTGTCGGGCTACCGCTGGGGCGTGGAGCGCAAGCGCGA
ATTGCTCGAGCGTGAAGGCAAACCACGGCAGTAA

Upstream 100 bases:

>100_bases
ACCGCTGGATAATCCTGCAAAGTTTGACCGCAAGGGTCGGAGTGTTCCGGCCGGTGGCACTGGTTAACCTGACATTCAAC
AGGTCAACGGACTCTCCATC

Downstream 100 bases:

>100_bases
TCAGCGATTCACATCAACTACAACTCGCCCCCGGAGTTGCCCCGCCAGCAACTTCGGGGCCGCTTCGAGGGCTTCGCCGA
GGCCGATTTCATGGCTGATC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKTLSTSLSTEDDPRWAAVVARDPRADGQFVYAVKTTGIYCRPSSLARLPKPQNVEFFDTAEDAEAAGYRPSKRASKDQT
EVAAQHAATVAAACRQIEASDSLPALNDLAETAGLSAFHFHRVFKAATGLTPKGYAAAHRSRRVRQRLADGSSVTEALYD
AGFNSNSRFYEAADQVLGMKPGDFRSAGQNNDIRFAVGQCSLGAILVAQSERGICAILLGDDPHQLVCDLQDQFRRANLI
GADTEFEQLIARVVGFIEAPAIGLDLPLDVRGTAFQERVWQALREIPVGSTASYADIALRIGSPKAVRAVAQACGANSLA
VAIPCHRVVRSDGNLSGYRWGVERKRELLEREGKPRQ

Sequences:

>Translated_357_residues
MKTLSTSLSTEDDPRWAAVVARDPRADGQFVYAVKTTGIYCRPSSLARLPKPQNVEFFDTAEDAEAAGYRPSKRASKDQT
EVAAQHAATVAAACRQIEASDSLPALNDLAETAGLSAFHFHRVFKAATGLTPKGYAAAHRSRRVRQRLADGSSVTEALYD
AGFNSNSRFYEAADQVLGMKPGDFRSAGQNNDIRFAVGQCSLGAILVAQSERGICAILLGDDPHQLVCDLQDQFRRANLI
GADTEFEQLIARVVGFIEAPAIGLDLPLDVRGTAFQERVWQALREIPVGSTASYADIALRIGSPKAVRAVAQACGANSLA
VAIPCHRVVRSDGNLSGYRWGVERKRELLEREGKPRQ
>Mature_357_residues
MKTLSTSLSTEDDPRWAAVVARDPRADGQFVYAVKTTGIYCRPSSLARLPKPQNVEFFDTAEDAEAAGYRPSKRASKDQT
EVAAQHAATVAAACRQIEASDSLPALNDLAETAGLSAFHFHRVFKAATGLTPKGYAAAHRSRRVRQRLADGSSVTEALYD
AGFNSNSRFYEAADQVLGMKPGDFRSAGQNNDIRFAVGQCSLGAILVAQSERGICAILLGDDPHQLVCDLQDQFRRANLI
GADTEFEQLIARVVGFIEAPAIGLDLPLDVRGTAFQERVWQALREIPVGSTASYADIALRIGSPKAVRAVAQACGANSLA
VAIPCHRVVRSDGNLSGYRWGVERKRELLEREGKPRQ

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=343, Percent_Identity=51.8950437317784, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=45.4545454545455, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=60.4938271604938, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=60.4938271604938, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=48.1481481481481, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137554, Length=87, Percent_Identity=48.2758620689655, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 38645; Mature: 38645

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLSTSLSTEDDPRWAAVVARDPRADGQFVYAVKTTGIYCRPSSLARLPKPQNVEFFDT
CCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCEEECCHHHHCCCCCCCCEEEEC
AEDAEAAGYRPSKRASKDQTEVAAQHAATVAAACRQIEASDSLPALNDLAETAGLSAFHF
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHH
HRVFKAATGLTPKGYAAAHRSRRVRQRLADGSSVTEALYDAGFNSNSRFYEAADQVLGMK
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PGDFRSAGQNNDIRFAVGQCSLGAILVAQSERGICAILLGDDPHQLVCDLQDQFRRANLI
CCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHC
GADTEFEQLIARVVGFIEAPAIGLDLPLDVRGTAFQERVWQALREIPVGSTASYADIALR
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEE
IGSPKAVRAVAQACGANSLAVAIPCHRVVRSDGNLSGYRWGVERKRELLEREGKPRQ
CCCCHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEECCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKTLSTSLSTEDDPRWAAVVARDPRADGQFVYAVKTTGIYCRPSSLARLPKPQNVEFFDT
CCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCEEECCHHHHCCCCCCCCEEEEC
AEDAEAAGYRPSKRASKDQTEVAAQHAATVAAACRQIEASDSLPALNDLAETAGLSAFHF
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHH
HRVFKAATGLTPKGYAAAHRSRRVRQRLADGSSVTEALYDAGFNSNSRFYEAADQVLGMK
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PGDFRSAGQNNDIRFAVGQCSLGAILVAQSERGICAILLGDDPHQLVCDLQDQFRRANLI
CCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHC
GADTEFEQLIARVVGFIEAPAIGLDLPLDVRGTAFQERVWQALREIPVGSTASYADIALR
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEE
IGSPKAVRAVAQACGANSLAVAIPCHRVVRSDGNLSGYRWGVERKRELLEREGKPRQ
CCCCHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEECCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]