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The map label for this gene is ahpF [H]

Identifier: 77459149

GI number: 77459149

Start: 3369161

End: 3370723

Strand: Reverse

Name: ahpF [H]

Synonym: Pfl01_2924

Alternate gene names: 77459149

Gene position: 3370723-3369161 (Counterclockwise)

Preceding gene: 77459150

Following gene: 77459147

Centisome position: 52.35

GC content: 60.78

Gene sequence:

>1563_bases
ATGTTGGACGCCAATCTTAAAGCCCAGTTGAAATCGTACCTGGAACGGGTCACCCAGCCGATCGAGATCGTTGCTTCACT
CGACGACGGTGCGAAATCCCGTGAAATGCTCGACCTGCTGAAAGACATTGCCAGTCTTTCGCATCAGATTACCTTGATCG
ACAGCGGCGACGATGCACGCAAACCGTCGTTCTCGATCAACCGCCCCGGGGCCGATATCAGCCTGCGTTTCGCCGGCATC
CCGATGGGCCATGAATTCACTTCGCTGGTGCTGGCCCTGCTGCAAGTCGGCGGCCACCCTTCGAAGGCCAGTGTCGAAGT
GATCGAACAGATCCGCTCGCTCAAGGGCGAGTTCAACTTCGAGACGTACTTCTCGCTGTCCTGCCAGAACTGCCCGGACG
TTGTTCAGGCATTGAACCTGATGGCCGTGCTGAACCCGAATATCCGCCACGTCGCCATCGACGGCGCGCTGTTCCAGGAC
GAAGTCAACGATCGCAAGATCATGGCTGTGCCGAGCATTTACCTGAACGGCGAAAACTTCGGCCAGGGCCGTATGGGCCT
GGAAGAAATCCTCGCCAAACTCGACACTGGCGCCATCGAGCGTCAGGCGGAGAAAATCAGCGCCAAGCAAGCCTTCGATG
TGCTGGTGGTTGGCGGTGGCCCGGCCGGTGCTTCGGCGGCGATCTATGCCGCACGTAAAGGCATTCGCACCGGTGTGGCG
GCCGAGCGCTTCGGCGGTCAGGTGCTGGACACCATGGCCATCGAGAACTTCATTTCCGTGCAGGAAACCGAAGGCCCGAA
ACTGGCGACCGCGCTGGAAGAGCACGTCAAGCAGTACGACGTCGACATCATGAACCTGCAACGCGCCGACAAGCTGATCC
CGGGCAAGAATGGCGAGCTGCATGAAGTTCGCTTCGCCAGCGGCGCGACCCTCAAGGCCAAGAGCGTGATTCTGGCCACC
GGTGCGCGGTGGCGTGAAATGAACGTGCCGGGCGAACAGGAATACCGTAACAAAGGCGTGGCGTACTGCCCGCACTGCGA
CGGCCCGCTGTTCAAAGGCAAGCGTGTGGCGGTGATTGGCGGCGGTAACTCCGGCGTCGAAGCGGCCATTGACCTGGCCG
GTATCGTCTCCCACGTGACTCTGCTGGAGTTCGACGTTCAGTTGCGCGCCGACGCCGTATTGCAACGCAAGCTGCACAGC
CTGCCGAACGTCACCGTGATCACCAGTGCGCTGACCACTGAAGTCACTGGCGACGGGCAGAAGGTCAACGGCCTGCGCTA
CAAGGATCGCAATACTGACGAGCTGCGCACTGTCGAACTGGAAGGGATTTTCGTGCAGATCGGTCTGCTGCCCAACACCG
ACTGGCTCAAAGGCACCATCGAGCTGTCGCCGCGCGGCGAGATCATCGTCGACAACCGCGGCGAAACCTCGATCCCCGGC
ATCTTCGCCGCCGGTGACGTGACCACCGTGCCGTACAAGCAGATCGTGATCGCAGTGGGCGAGGGTGCCAAGGCTTCCCT
CAGTGCTTTCGATCACCTGATCCGCACGTCGGCTCCGGCGTAA

Upstream 100 bases:

>100_bases
TCCGCACCTACTCAAGTAAGCTGCACCGCCCAATAGAAAAGCCCGGGCGAGATTCGCTCGGGCTTTTTTTCGCCTCAAAT
AAAGTCAGGAGATCGCCCGT

Downstream 100 bases:

>100_bases
ATCCAGGGTCTGAAAAAGAAAACCCCCATCAGTGATGATGGGGGTTTTTCTTTGCGTGATGCTTTCTACCTGCGTTCAGC
TTTACAGCGGCGCCGGCTGG

Product: pyridine nucleotide-disulfide oxidoreductase class-II

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MLDANLKAQLKSYLERVTQPIEIVASLDDGAKSREMLDLLKDIASLSHQITLIDSGDDARKPSFSINRPGADISLRFAGI
PMGHEFTSLVLALLQVGGHPSKASVEVIEQIRSLKGEFNFETYFSLSCQNCPDVVQALNLMAVLNPNIRHVAIDGALFQD
EVNDRKIMAVPSIYLNGENFGQGRMGLEEILAKLDTGAIERQAEKISAKQAFDVLVVGGGPAGASAAIYAARKGIRTGVA
AERFGGQVLDTMAIENFISVQETEGPKLATALEEHVKQYDVDIMNLQRADKLIPGKNGELHEVRFASGATLKAKSVILAT
GARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHS
LPNVTVITSALTTEVTGDGQKVNGLRYKDRNTDELRTVELEGIFVQIGLLPNTDWLKGTIELSPRGEIIVDNRGETSIPG
IFAAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRTSAPA

Sequences:

>Translated_520_residues
MLDANLKAQLKSYLERVTQPIEIVASLDDGAKSREMLDLLKDIASLSHQITLIDSGDDARKPSFSINRPGADISLRFAGI
PMGHEFTSLVLALLQVGGHPSKASVEVIEQIRSLKGEFNFETYFSLSCQNCPDVVQALNLMAVLNPNIRHVAIDGALFQD
EVNDRKIMAVPSIYLNGENFGQGRMGLEEILAKLDTGAIERQAEKISAKQAFDVLVVGGGPAGASAAIYAARKGIRTGVA
AERFGGQVLDTMAIENFISVQETEGPKLATALEEHVKQYDVDIMNLQRADKLIPGKNGELHEVRFASGATLKAKSVILAT
GARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHS
LPNVTVITSALTTEVTGDGQKVNGLRYKDRNTDELRTVELEGIFVQIGLLPNTDWLKGTIELSPRGEIIVDNRGETSIPG
IFAAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRTSAPA
>Mature_520_residues
MLDANLKAQLKSYLERVTQPIEIVASLDDGAKSREMLDLLKDIASLSHQITLIDSGDDARKPSFSINRPGADISLRFAGI
PMGHEFTSLVLALLQVGGHPSKASVEVIEQIRSLKGEFNFETYFSLSCQNCPDVVQALNLMAVLNPNIRHVAIDGALFQD
EVNDRKIMAVPSIYLNGENFGQGRMGLEEILAKLDTGAIERQAEKISAKQAFDVLVVGGGPAGASAAIYAARKGIRTGVA
AERFGGQVLDTMAIENFISVQETEGPKLATALEEHVKQYDVDIMNLQRADKLIPGKNGELHEVRFASGATLKAKSVILAT
GARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHS
LPNVTVITSALTTEVTGDGQKVNGLRYKDRNTDELRTVELEGIFVQIGLLPNTDWLKGTIELSPRGEIIVDNRGETSIPG
IFAAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRTSAPA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=518, Percent_Identity=65.2509652509653, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=318, Percent_Identity=34.9056603773585, Blast_Score=172, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6321898, Length=313, Percent_Identity=34.185303514377, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6320560, Length=313, Percent_Identity=32.9073482428115, Blast_Score=157, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 55999; Mature: 55999

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANLKAQLKSYLERVTQPIEIVASLDDGAKSREMLDLLKDIASLSHQITLIDSGDDAR
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCC
KPSFSINRPGADISLRFAGIPMGHEFTSLVLALLQVGGHPSKASVEVIEQIRSLKGEFNF
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCE
ETYFSLSCQNCPDVVQALNLMAVLNPNIRHVAIDGALFQDEVNDRKIMAVPSIYLNGENF
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCEEEEECEEEECCCCC
GQGRMGLEEILAKLDTGAIERQAEKISAKQAFDVLVVGGGPAGASAAIYAARKGIRTGVA
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCHHCCCH
AERFGGQVLDTMAIENFISVQETEGPKLATALEEHVKQYDVDIMNLQRADKLIPGKNGEL
HHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCE
HEVRFASGATLKAKSVILATGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKRVAVIG
EEEEECCCCCEEECEEEEECCCCEEECCCCCHHHHHCCCCEECCCCCCCCCCCCEEEEEE
GGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHSLPNVTVITSALTTEVTGDGQ
CCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCEEEEEHHHEEEECCCCC
KVNGLRYKDRNTDELRTVELEGIFVQIGLLPNTDWLKGTIELSPRGEIIVDNRGETSIPG
EECCEEECCCCCCCEEEEEEEEEEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCCCC
IFAAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRTSAPA
EEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLDANLKAQLKSYLERVTQPIEIVASLDDGAKSREMLDLLKDIASLSHQITLIDSGDDAR
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCC
KPSFSINRPGADISLRFAGIPMGHEFTSLVLALLQVGGHPSKASVEVIEQIRSLKGEFNF
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCE
ETYFSLSCQNCPDVVQALNLMAVLNPNIRHVAIDGALFQDEVNDRKIMAVPSIYLNGENF
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCEEEEECEEEECCCCC
GQGRMGLEEILAKLDTGAIERQAEKISAKQAFDVLVVGGGPAGASAAIYAARKGIRTGVA
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCHHCCCH
AERFGGQVLDTMAIENFISVQETEGPKLATALEEHVKQYDVDIMNLQRADKLIPGKNGEL
HHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCE
HEVRFASGATLKAKSVILATGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKRVAVIG
EEEEECCCCCEEECEEEEECCCCEEECCCCCHHHHHCCCCEECCCCCCCCCCCCEEEEEE
GGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHSLPNVTVITSALTTEVTGDGQ
CCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCEEEEEHHHEEEECCCCC
KVNGLRYKDRNTDELRTVELEGIFVQIGLLPNTDWLKGTIELSPRGEIIVDNRGETSIPG
EECCEEECCCCCCCEEEEEEEEEEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCCCC
IFAAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRTSAPA
EEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]