Click here to switch to the map view.

The map label for this gene is rstB [H]

Identifier: 77459116

GI number: 77459116

Start: 3330647

End: 3331948

Strand: Reverse

Name: rstB [H]

Synonym: Pfl01_2891

Alternate gene names: 77459116

Gene position: 3331948-3330647 (Counterclockwise)

Preceding gene: 77459117

Following gene: 77459115

Centisome position: 51.75

GC content: 62.67

Gene sequence:

>1302_bases
ATGTTCAGAATCCTGTTTCGCCTCTATCTGGTGACGATCGTCTCCTACAGTGCGGCGATCTATCTGGTGCCGGATCTGGT
GGTGATGGTGTTCAAGCAGCGGTTCGTCACCTACAACCTCGACTATTCCCGTGGTCTGCAATCGCTGATCACCAAGCAGT
TTCGCGCGGTGCCGCAGGATCAGTGGCCGGCGCTCGCGGCGTCGATGGACAAGGACTTCCAGCCGCTGCACATCGTGCTC
GCCCGTATCGATGACCCCGGCTTCACCGCCATCGAGCAAGAGCGGCTGCGGCGCGGAGAGAACATGGTGCGGGTGGGCGA
CTGGGGCTGGCGCACGTTGGCGGTCACGCCACTGAACGACGAAATGGCGGTGCAGATGGTGGTGCCGCCGGATCCGATGG
ACGTGAACCTGTTGTACTGGAGCATCAACGTGCTGATCGGCGCGAGTCTGCTGGCGTGCCTGTTGCTCTGGTTTCGTCCG
CACTGGCGCGATCTGGAACGCCTCAAGGGCACGGCCGAACGTTTCGGCAAGGGCCATTTGAGCGAGCGTACGCAGATTGG
CCCGAGCTCCAACATCGGCAGCCTGGCCCACGTGTTCGACACCATGGCCGGCGACATCGAAAACCTGCTCAACCAGCAAC
GCGATCTGCTCAACGCCGTGTCCCACGAACTCCGCACGCCGCTGACCCGCCTGGACTTCGGCCTGGCGCTGGCGCTGTCC
GATGACTTGCCGCAAGCCAGCCGCGAACGCTTGCAAGGGCTGGTCGCGCACATTCGTGAACTGGATGAACTGGTGCTGGA
GTTGCTGTCCTACAGTCGCCTGCAGAATCCGGCGAAGTTGCCGGAGCAGGTCGAAGTGTCGCTGGACGAGTTCATCGACA
GCATTCTGGGCAGCGTCGATGAAGAACTGGAATCCCCGGAAATCGTCATCGATGTGTTGCTGCACAATCAGTTGGAACGC
TTCTCCCTCGACCCGCGCTTGACGGCCCGCGCGATTCAGAACCTGCTGCGCAACGCCATGCGCTACTGCGAAAAGCGCAT
TCAGATTGGCGTGCAGGTCTGCCCGAAGGGCTGCGAGATCTGGGTGGACGATGACGGAATCGGCATTCCGGAAGACGAGC
GGGAGCGGATTTTCGAGCCGTTCTATCGACTGGACCGCAGCCGCGATCGGGCGACCGGCGGGTTTGGCCTGGGGCTGGCG
ATCAGCCGGCGGGCGCTGGAAGCCCAAGGCGGGACGTTGACGGTGGAAGGTTCGCCGTTGGGTGGGGCGAGGTTCAGGCT
CTGGCTGCCAACCCCGACCTGA

Upstream 100 bases:

>100_bases
CGACGCAAGTTCGACGACCACGCCGGCGAGGCGCGCAAGATCAAGACCGTGTGGGGCAAGGGGTATCTGTTCAGCCGTTC
CGAGTGGGAATGCTGATTCG

Downstream 100 bases:

>100_bases
TGATCGTTCCGTACGCCGACAACGAGGCGGCCATCGGCCTCTACCGCAAGTTCGGCTTCGAGACCGCGGGCCTGTTTCGC
GACTACGCCGTGCGCGACGG

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MFRILFRLYLVTIVSYSAAIYLVPDLVVMVFKQRFVTYNLDYSRGLQSLITKQFRAVPQDQWPALAASMDKDFQPLHIVL
ARIDDPGFTAIEQERLRRGENMVRVGDWGWRTLAVTPLNDEMAVQMVVPPDPMDVNLLYWSINVLIGASLLACLLLWFRP
HWRDLERLKGTAERFGKGHLSERTQIGPSSNIGSLAHVFDTMAGDIENLLNQQRDLLNAVSHELRTPLTRLDFGLALALS
DDLPQASRERLQGLVAHIRELDELVLELLSYSRLQNPAKLPEQVEVSLDEFIDSILGSVDEELESPEIVIDVLLHNQLER
FSLDPRLTARAIQNLLRNAMRYCEKRIQIGVQVCPKGCEIWVDDDGIGIPEDERERIFEPFYRLDRSRDRATGGFGLGLA
ISRRALEAQGGTLTVEGSPLGGARFRLWLPTPT

Sequences:

>Translated_433_residues
MFRILFRLYLVTIVSYSAAIYLVPDLVVMVFKQRFVTYNLDYSRGLQSLITKQFRAVPQDQWPALAASMDKDFQPLHIVL
ARIDDPGFTAIEQERLRRGENMVRVGDWGWRTLAVTPLNDEMAVQMVVPPDPMDVNLLYWSINVLIGASLLACLLLWFRP
HWRDLERLKGTAERFGKGHLSERTQIGPSSNIGSLAHVFDTMAGDIENLLNQQRDLLNAVSHELRTPLTRLDFGLALALS
DDLPQASRERLQGLVAHIRELDELVLELLSYSRLQNPAKLPEQVEVSLDEFIDSILGSVDEELESPEIVIDVLLHNQLER
FSLDPRLTARAIQNLLRNAMRYCEKRIQIGVQVCPKGCEIWVDDDGIGIPEDERERIFEPFYRLDRSRDRATGGFGLGLA
ISRRALEAQGGTLTVEGSPLGGARFRLWLPTPT
>Mature_433_residues
MFRILFRLYLVTIVSYSAAIYLVPDLVVMVFKQRFVTYNLDYSRGLQSLITKQFRAVPQDQWPALAASMDKDFQPLHIVL
ARIDDPGFTAIEQERLRRGENMVRVGDWGWRTLAVTPLNDEMAVQMVVPPDPMDVNLLYWSINVLIGASLLACLLLWFRP
HWRDLERLKGTAERFGKGHLSERTQIGPSSNIGSLAHVFDTMAGDIENLLNQQRDLLNAVSHELRTPLTRLDFGLALALS
DDLPQASRERLQGLVAHIRELDELVLELLSYSRLQNPAKLPEQVEVSLDEFIDSILGSVDEELESPEIVIDVLLHNQLER
FSLDPRLTARAIQNLLRNAMRYCEKRIQIGVQVCPKGCEIWVDDDGIGIPEDERERIFEPFYRLDRSRDRATGGFGLGLA
ISRRALEAQGGTLTVEGSPLGGARFRLWLPTPT

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=436, Percent_Identity=33.0275229357798, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1790346, Length=266, Percent_Identity=34.9624060150376, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788393, Length=268, Percent_Identity=29.8507462686567, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789808, Length=277, Percent_Identity=28.8808664259928, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=35.5371900826446, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=31.6017316017316, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786783, Length=237, Percent_Identity=28.6919831223629, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=30.1369863013699, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082128, Length=299, Percent_Identity=26.7558528428094, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693157, Length=231, Percent_Identity=26.4069264069264, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789149, Length=225, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788549, Length=219, Percent_Identity=27.3972602739726, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081816, Length=223, Percent_Identity=30.9417040358744, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787374, Length=308, Percent_Identity=23.7012987012987, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789403, Length=237, Percent_Identity=27.8481012658228, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994928, Length=235, Percent_Identity=26.3829787234043, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788279, Length=295, Percent_Identity=24.0677966101695, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49138; Mature: 49138

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRILFRLYLVTIVSYSAAIYLVPDLVVMVFKQRFVTYNLDYSRGLQSLITKQFRAVPQD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCC
QWPALAASMDKDFQPLHIVLARIDDPGFTAIEQERLRRGENMVRVGDWGWRTLAVTPLND
CCCHHHHHCCCCCCCHHHEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCEEEEEEECCC
EMAVQMVVPPDPMDVNLLYWSINVLIGASLLACLLLWFRPHWRDLERLKGTAERFGKGHL
CEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
SERTQIGPSSNIGSLAHVFDTMAGDIENLLNQQRDLLNAVSHELRTPLTRLDFGLALALS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCEEEEEC
DDLPQASRERLQGLVAHIRELDELVLELLSYSRLQNPAKLPEQVEVSLDEFIDSILGSVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EELESPEIVIDVLLHNQLERFSLDPRLTARAIQNLLRNAMRYCEKRIQIGVQVCPKGCEI
HHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
WVDDDGIGIPEDERERIFEPFYRLDRSRDRATGGFGLGLAISRRALEAQGGTLTVEGSPL
EECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCC
GGARFRLWLPTPT
CCCEEEEEECCCC
>Mature Secondary Structure
MFRILFRLYLVTIVSYSAAIYLVPDLVVMVFKQRFVTYNLDYSRGLQSLITKQFRAVPQD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCC
QWPALAASMDKDFQPLHIVLARIDDPGFTAIEQERLRRGENMVRVGDWGWRTLAVTPLND
CCCHHHHHCCCCCCCHHHEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCEEEEEEECCC
EMAVQMVVPPDPMDVNLLYWSINVLIGASLLACLLLWFRPHWRDLERLKGTAERFGKGHL
CEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
SERTQIGPSSNIGSLAHVFDTMAGDIENLLNQQRDLLNAVSHELRTPLTRLDFGLALALS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCEEEEEC
DDLPQASRERLQGLVAHIRELDELVLELLSYSRLQNPAKLPEQVEVSLDEFIDSILGSVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EELESPEIVIDVLLHNQLERFSLDPRLTARAIQNLLRNAMRYCEKRIQIGVQVCPKGCEI
HHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
WVDDDGIGIPEDERERIFEPFYRLDRSRDRATGGFGLGLAISRRALEAQGGTLTVEGSPL
EECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCC
GGARFRLWLPTPT
CCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]