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The map label for this gene is yngF [H]

Identifier: 77459089

GI number: 77459089

Start: 3303890

End: 3304996

Strand: Direct

Name: yngF [H]

Synonym: Pfl01_2864

Alternate gene names: 77459089

Gene position: 3303890-3304996 (Clockwise)

Preceding gene: 77459088

Following gene: 77459090

Centisome position: 51.32

GC content: 63.41

Gene sequence:

>1107_bases
ATGACTGCTCAGGCTTCATCCCCGCGGACTTCGTCCATGGATGCCACGCCACACGAAGTGCTGGCCGAGGTTCGCAATCA
CATCGGTCATCTGACCCTCAACCGCCCCGCCGGCCTCAATGCCCTGACCCTCGACATGGTGCGCCAGCTCCAGCAATACC
TGGACGCCTGGGCCGCCGATGCCGATATCCACGCGGTTGTTCTGCGCGGTGCCGGCGAGAAAGCCTTCTGTGCCGGTGGC
GACATTCGATCGCTGTACGACAGCCATAAAAACGGCGATACGCTGCACGAAGATTTCTTCGTCGAGGAATACGCCCTCGA
CCTGACGATCCACCATTACCGCAAACCGGTGCTGGCGCTGATGGACGGTTTCGTCCTCGGCGGCGGCATGGGCCTGGTGC
AAGGCGCTGATCTGCGGGTGGTCACCGAGAAGAGCCGTCTGGCGATGCCGGAAGTGGCGATCGGCTATTTCCCGGATGTC
GGCGGCAGTTATTTCCTGCCACGGATTCCCGGCGAGCTGGGGATTTATCTGGGCGTCAGCGGCGTGCAGATCCGTGCGGC
GGATGCGCTGTATTGCGGCCTCGCCGACTGGTATCTGGAGAGCAGCAAACTCGGCATCCTCGACGAACAACTCGATCACC
TGGAGTGGCACGACACGCCGCTCAAAGACCTGCAAAGCCTGCTGGCCCGCCACGCCGTGCAAACCCTGCCGGATGCGCCG
CTGGAAGCCTTGCGCCCGGCCATCGATCACTTCTTCGCGTTGCCGGACGTGCCGAGCATTGTCGAGCAACTGCGTGCCGT
GACCGTCGCCGACAGCCACGAATGGGCGACCACTACCGCCGACCTGCTGGAAACCCGCTCGCCGCTGGCCATGGGCGTGA
CCCTGGAAATGCTGCGTCGCGGCCGGCACCTGAGCCTGGAACACTGTTTCGCCCTCGAACTGCATCTGGATCGCCAGTGG
TTCGAACGCGGCGACCTGATCGAAGGCGTACGCGCCCTGCTGATCGACAAAGACAAATCACCGCGCTGGAACCCGCCGAC
CCTGGCTGCGCTGCACGCCGAGCAGGTCGAAAGTTTCTTCCACGGTTTCGCTGAAAGCGGGAGCTGA

Upstream 100 bases:

>100_bases
CACATATCCCCGTTGTACACGGCAAATTTGCACTGTTACGATCCGGTAACGCTCGCGCGCGAGCTTCTCGAATAAAGACA
ATAAAAGCAGGGAGTTAGTG

Downstream 100 bases:

>100_bases
GCCATGCACGATCTCGAATTGACTGAAGACCAGGTAATGATCCGCGACATGGCCCGGGACTTTGCGCGCGGCGAAATCGC
GCCCCACGCGCAAGCCTGGG

Product: enoyl-CoA hydratase/isomerase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTAQASSPRTSSMDATPHEVLAEVRNHIGHLTLNRPAGLNALTLDMVRQLQQYLDAWAADADIHAVVLRGAGEKAFCAGG
DIRSLYDSHKNGDTLHEDFFVEEYALDLTIHHYRKPVLALMDGFVLGGGMGLVQGADLRVVTEKSRLAMPEVAIGYFPDV
GGSYFLPRIPGELGIYLGVSGVQIRAADALYCGLADWYLESSKLGILDEQLDHLEWHDTPLKDLQSLLARHAVQTLPDAP
LEALRPAIDHFFALPDVPSIVEQLRAVTVADSHEWATTTADLLETRSPLAMGVTLEMLRRGRHLSLEHCFALELHLDRQW
FERGDLIEGVRALLIDKDKSPRWNPPTLAALHAEQVESFFHGFAESGS

Sequences:

>Translated_368_residues
MTAQASSPRTSSMDATPHEVLAEVRNHIGHLTLNRPAGLNALTLDMVRQLQQYLDAWAADADIHAVVLRGAGEKAFCAGG
DIRSLYDSHKNGDTLHEDFFVEEYALDLTIHHYRKPVLALMDGFVLGGGMGLVQGADLRVVTEKSRLAMPEVAIGYFPDV
GGSYFLPRIPGELGIYLGVSGVQIRAADALYCGLADWYLESSKLGILDEQLDHLEWHDTPLKDLQSLLARHAVQTLPDAP
LEALRPAIDHFFALPDVPSIVEQLRAVTVADSHEWATTTADLLETRSPLAMGVTLEMLRRGRHLSLEHCFALELHLDRQW
FERGDLIEGVRALLIDKDKSPRWNPPTLAALHAEQVESFFHGFAESGS
>Mature_367_residues
TAQASSPRTSSMDATPHEVLAEVRNHIGHLTLNRPAGLNALTLDMVRQLQQYLDAWAADADIHAVVLRGAGEKAFCAGGD
IRSLYDSHKNGDTLHEDFFVEEYALDLTIHHYRKPVLALMDGFVLGGGMGLVQGADLRVVTEKSRLAMPEVAIGYFPDVG
GSYFLPRIPGELGIYLGVSGVQIRAADALYCGLADWYLESSKLGILDEQLDHLEWHDTPLKDLQSLLARHAVQTLPDAPL
EALRPAIDHFFALPDVPSIVEQLRAVTVADSHEWATTTADLLETRSPLAMGVTLEMLRRGRHLSLEHCFALELHLDRQWF
ERGDLIEGVRALLIDKDKSPRWNPPTLAALHAEQVESFFHGFAESGS

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=363, Percent_Identity=37.1900826446281, Blast_Score=242, Evalue=5e-64,
Organism=Homo sapiens, GI37594469, Length=315, Percent_Identity=36.8253968253968, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI194097323, Length=184, Percent_Identity=34.2391304347826, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI68989263, Length=304, Percent_Identity=27.3026315789474, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI70995211, Length=184, Percent_Identity=29.3478260869565, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI31542718, Length=191, Percent_Identity=25.130890052356, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1787659, Length=178, Percent_Identity=31.4606741573034, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI221142681, Length=188, Percent_Identity=29.7872340425532, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788597, Length=147, Percent_Identity=30.6122448979592, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=26.5625, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788682, Length=175, Percent_Identity=29.1428571428571, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787660, Length=233, Percent_Identity=29.1845493562232, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=351, Percent_Identity=39.031339031339, Blast_Score=256, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI25144157, Length=351, Percent_Identity=39.031339031339, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI25145438, Length=169, Percent_Identity=34.3195266272189, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=171, Percent_Identity=30.4093567251462, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=164, Percent_Identity=29.2682926829268, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=144, Percent_Identity=29.8611111111111, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=157, Percent_Identity=31.8471337579618, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=189, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=164, Percent_Identity=26.219512195122, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=364, Percent_Identity=28.5714285714286, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI28571729, Length=368, Percent_Identity=39.945652173913, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI28571730, Length=368, Percent_Identity=39.945652173913, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI20129971, Length=240, Percent_Identity=31.25, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24653477, Length=240, Percent_Identity=31.25, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24653139, Length=157, Percent_Identity=32.484076433121, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920382, Length=181, Percent_Identity=30.3867403314917, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 40634; Mature: 40502

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQASSPRTSSMDATPHEVLAEVRNHIGHLTLNRPAGLNALTLDMVRQLQQYLDAWAAD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
ADIHAVVLRGAGEKAFCAGGDIRSLYDSHKNGDTLHEDFFVEEYALDLTIHHYRKPVLAL
CCEEEEEEECCCCCEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHEEHHHHHHHHHHH
MDGFVLGGGMGLVQGADLRVVTEKSRLAMPEVAIGYFPDVGGSYFLPRIPGELGIYLGVS
HCCHHHCCCCCEECCCCEEEEECCHHHCCCCHHCCCCCCCCCCEECCCCCCCEEEEEECC
GVQIRAADALYCGLADWYLESSKLGILDEQLDHLEWHDTPLKDLQSLLARHAVQTLPDAP
CEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
LEALRPAIDHFFALPDVPSIVEQLRAVTVADSHEWATTTADLLETRSPLAMGVTLEMLRR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
GRHLSLEHCFALELHLDRQWFERGDLIEGVRALLIDKDKSPRWNPPTLAALHAEQVESFF
CCCCCHHHEEEEEEECCHHHHHCCCHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHH
HGFAESGS
HHHHCCCC
>Mature Secondary Structure 
TAQASSPRTSSMDATPHEVLAEVRNHIGHLTLNRPAGLNALTLDMVRQLQQYLDAWAAD
CCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
ADIHAVVLRGAGEKAFCAGGDIRSLYDSHKNGDTLHEDFFVEEYALDLTIHHYRKPVLAL
CCEEEEEEECCCCCEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHEEHHHHHHHHHHH
MDGFVLGGGMGLVQGADLRVVTEKSRLAMPEVAIGYFPDVGGSYFLPRIPGELGIYLGVS
HCCHHHCCCCCEECCCCEEEEECCHHHCCCCHHCCCCCCCCCCEECCCCCCCEEEEEECC
GVQIRAADALYCGLADWYLESSKLGILDEQLDHLEWHDTPLKDLQSLLARHAVQTLPDAP
CEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
LEALRPAIDHFFALPDVPSIVEQLRAVTVADSHEWATTTADLLETRSPLAMGVTLEMLRR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
GRHLSLEHCFALELHLDRQWFERGDLIEGVRALLIDKDKSPRWNPPTLAALHAEQVESFF
CCCCCHHHEEEEEEECCHHHHHCCCHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHH
HGFAESGS
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]