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The map label for this gene is puuA [H]

Identifier: 77459053

GI number: 77459053

Start: 3260705

End: 3262111

Strand: Direct

Name: puuA [H]

Synonym: Pfl01_2828

Alternate gene names: 77459053

Gene position: 3260705-3262111 (Clockwise)

Preceding gene: 77459051

Following gene: 77459054

Centisome position: 50.64

GC content: 61.55

Gene sequence:

>1407_bases
ATGAAATCGCTTTTCCCCCGCGTCAACAAAACCAACGACGAGGTCTTTATGAATGCCCCTTTCGATCAGCTGTTCACGTG
GCTGAAAGATCACAAGATTACCGAAGTGGAATGCGTGGTCAGCGACCTGACCGGCATCGCCCGCGGCAAGATCGCACCGA
CCAACAAGTTCCTGCATGAGCGAGGCATGCGCCTGCCGGAAAGTGTGCTGCTGCAAACGGTAACCGGGGATTTTGTCGAC
GACGACATCTACTACGACCTGCTCGACCCGGCCGACATCGACATGGTCTGCAAACCGGTGAGCGACGCGGTGTACGTGAT
CCCGTGGGCCATCGAGCCGACCGCCATCGTCATCCACGACACCTTCGACAAGTTCGGCAACCCGATCGAACTGTCGCCGC
GCAACGTGCTGAAGAAAGTCCTGCAGCTGTACACCGACAAAGGCTGGAAGCCGATCGTGGCGCCGGAAATGGAGTTCTAC
CTGACCCAGCGCTGCGAAGACCCGGACTTGCCATTGAAAGCACCGCTGGGCCGTTCCGGTCGCGCCGAAAGCGGTCGTCA
GTCGTTCTCCATCGACGCCGCCAACGAATTCGATCCATTGTTCGAAGACGTCTACGACTGGTGCGAACTGCAAGGCCTGG
ACCTGGACACGCTGATCCACGAAGACGGCCCGGCGCAGATGGAAATCAACTTCCGCCACGGCGACGCGCTCGATCTGGCC
GACCAGATCACCGTGTTCAAGCGTACCCTGCGTGAAGCCGCCCTCAAGCACAACGTGACCGCGACTTTCATGGCCAAGCC
GATCGGCGACGAGCCCGGCAGCGCCATGCACCTGCATCAGAGCGTGGTCGAGATCGCCAGCGGCCAGCCAATTTTCGCCA
ATGCCGACGGCAGCATGAGCGACCTGTTCCGCCATCACATCGGCGGCCTGCAGAAGTACATTCCGAAGGTGCTGCCGATG
TTCGCGCCGAACGTGAACTCGTTCCGCCGCTTCCTGCCGGACACCTCGGCACCGGTCAACGTCGAATGGGGCGAAGAAAA
CCGCACCGTCGGCCTGCGGGTGCCAACCTCCGGACCTGAGGCGATGCGGGTTGAGAACCGTCTGCCCGGCGCCGACGCCA
ACCCGTACCTGGCCATCGCCGCGAGCCTGCTATGCGGCTACATCGGTATGGTCGAAGGCGTCGAACCGAGCGCCGCCGTC
GAAGGCCGGGCTTACGAGCGCCGCAACCTGCGCCTGCCGATCACCATCGAAGAAGCCCTGACCCAGATGGAAGAGTGCGA
CACCGTCGCGCAATACCTGGGCAGCAAGTTCGTACGCGGCTACGTCGCGGTGAAACGCGCCGAGCACGAAAACTTCAAGC
GCGTGATCAGTTCCTGGGAGCGGGAGTTCCTGTTGCTGAGCGTTTAA

Upstream 100 bases:

>100_bases
ACTCTTTTGGTGCGCTTTGGTGCTGCTGAAAGTGGATAGATGGAACGGCAAGGCCCGCCCAAGATAGTCGTCAACGCGCC
ACAGGGGAAGCCTGCGAAAG

Downstream 100 bases:

>100_bases
GCCTGAGACCTGATCGCTCCCGGACACTGTCCGGGAGCGATCCCTACCGACTTTACGAGCATAAAAAATCCAACTCGAAG
AGGTGTCGATATGCGTCTGT

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKSLFPRVNKTNDEVFMNAPFDQLFTWLKDHKITEVECVVSDLTGIARGKIAPTNKFLHERGMRLPESVLLQTVTGDFVD
DDIYYDLLDPADIDMVCKPVSDAVYVIPWAIEPTAIVIHDTFDKFGNPIELSPRNVLKKVLQLYTDKGWKPIVAPEMEFY
LTQRCEDPDLPLKAPLGRSGRAESGRQSFSIDAANEFDPLFEDVYDWCELQGLDLDTLIHEDGPAQMEINFRHGDALDLA
DQITVFKRTLREAALKHNVTATFMAKPIGDEPGSAMHLHQSVVEIASGQPIFANADGSMSDLFRHHIGGLQKYIPKVLPM
FAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPTSGPEAMRVENRLPGADANPYLAIAASLLCGYIGMVEGVEPSAAV
EGRAYERRNLRLPITIEEALTQMEECDTVAQYLGSKFVRGYVAVKRAEHENFKRVISSWEREFLLLSV

Sequences:

>Translated_468_residues
MKSLFPRVNKTNDEVFMNAPFDQLFTWLKDHKITEVECVVSDLTGIARGKIAPTNKFLHERGMRLPESVLLQTVTGDFVD
DDIYYDLLDPADIDMVCKPVSDAVYVIPWAIEPTAIVIHDTFDKFGNPIELSPRNVLKKVLQLYTDKGWKPIVAPEMEFY
LTQRCEDPDLPLKAPLGRSGRAESGRQSFSIDAANEFDPLFEDVYDWCELQGLDLDTLIHEDGPAQMEINFRHGDALDLA
DQITVFKRTLREAALKHNVTATFMAKPIGDEPGSAMHLHQSVVEIASGQPIFANADGSMSDLFRHHIGGLQKYIPKVLPM
FAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPTSGPEAMRVENRLPGADANPYLAIAASLLCGYIGMVEGVEPSAAV
EGRAYERRNLRLPITIEEALTQMEECDTVAQYLGSKFVRGYVAVKRAEHENFKRVISSWEREFLLLSV
>Mature_468_residues
MKSLFPRVNKTNDEVFMNAPFDQLFTWLKDHKITEVECVVSDLTGIARGKIAPTNKFLHERGMRLPESVLLQTVTGDFVD
DDIYYDLLDPADIDMVCKPVSDAVYVIPWAIEPTAIVIHDTFDKFGNPIELSPRNVLKKVLQLYTDKGWKPIVAPEMEFY
LTQRCEDPDLPLKAPLGRSGRAESGRQSFSIDAANEFDPLFEDVYDWCELQGLDLDTLIHEDGPAQMEINFRHGDALDLA
DQITVFKRTLREAALKHNVTATFMAKPIGDEPGSAMHLHQSVVEIASGQPIFANADGSMSDLFRHHIGGLQKYIPKVLPM
FAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPTSGPEAMRVENRLPGADANPYLAIAASLLCGYIGMVEGVEPSAAV
EGRAYERRNLRLPITIEEALTQMEECDTVAQYLGSKFVRGYVAVKRAEHENFKRVISSWEREFLLLSV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=424, Percent_Identity=26.1792452830189, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI87081870, Length=437, Percent_Identity=35.0114416475973, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1790301, Length=257, Percent_Identity=29.5719844357977, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 52513; Mature: 52513

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLFPRVNKTNDEVFMNAPFDQLFTWLKDHKITEVECVVSDLTGIARGKIAPTNKFLHE
CCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
RGMRLPESVLLQTVTGDFVDDDIYYDLLDPADIDMVCKPVSDAVYVIPWAIEPTAIVIHD
CCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEECCCCCEEEEEEECCCCEEEEEE
TFDKFGNPIELSPRNVLKKVLQLYTDKGWKPIVAPEMEFYLTQRCEDPDLPLKAPLGRSG
CHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
RAESGRQSFSIDAANEFDPLFEDVYDWCELQGLDLDTLIHEDGPAQMEINFRHGDALDLA
CCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHEECCCCCEEEEEECCCCCHHHH
DQITVFKRTLREAALKHNVTATFMAKPIGDEPGSAMHLHQSVVEIASGQPIFANADGSMS
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCHH
DLFRHHIGGLQKYIPKVLPMFAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPTSGPE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCH
AMRVENRLPGADANPYLAIAASLLCGYIGMVEGVEPSAAVEGRAYERRNLRLPITIEEAL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHCCCCEEEEEHHHHH
TQMEECDTVAQYLGSKFVRGYVAVKRAEHENFKRVISSWEREFLLLSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKSLFPRVNKTNDEVFMNAPFDQLFTWLKDHKITEVECVVSDLTGIARGKIAPTNKFLHE
CCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
RGMRLPESVLLQTVTGDFVDDDIYYDLLDPADIDMVCKPVSDAVYVIPWAIEPTAIVIHD
CCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEECCCCCEEEEEEECCCCEEEEEE
TFDKFGNPIELSPRNVLKKVLQLYTDKGWKPIVAPEMEFYLTQRCEDPDLPLKAPLGRSG
CHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
RAESGRQSFSIDAANEFDPLFEDVYDWCELQGLDLDTLIHEDGPAQMEINFRHGDALDLA
CCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHEECCCCCEEEEEECCCCCHHHH
DQITVFKRTLREAALKHNVTATFMAKPIGDEPGSAMHLHQSVVEIASGQPIFANADGSMS
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCHH
DLFRHHIGGLQKYIPKVLPMFAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPTSGPE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCH
AMRVENRLPGADANPYLAIAASLLCGYIGMVEGVEPSAAVEGRAYERRNLRLPITIEEAL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHCCCCEEEEEHHHHH
TQMEECDTVAQYLGSKFVRGYVAVKRAEHENFKRVISSWEREFLLLSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]