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The map label for this gene is potF [H]

Identifier: 77459054

GI number: 77459054

Start: 3262202

End: 3263290

Strand: Direct

Name: potF [H]

Synonym: Pfl01_2829

Alternate gene names: 77459054

Gene position: 3262202-3263290 (Clockwise)

Preceding gene: 77459053

Following gene: 77459055

Centisome position: 50.67

GC content: 58.77

Gene sequence:

>1089_bases
ATGCGTCTGTTGAAATCCATGATCCCCGCCGCGCTGGCCCTGGCGTGCAGTGCCGGTGCCTTCGCCCAACCCCAGGTCAG
CGTCTACAACTGGACCGATTACATTGGCGAAACCACCCTCGCCGACTTCCAGTCCTCCAGCGGGATCAAGGTGATCTACG
ACGTCTTCGACTCCAACGAAACCCTCGAAGGCAAACTGCTCGCCGGTCGCACCGGTTACGACGTGGTGGTGCCGTCCAAC
CACTTCCTCGCCCGTCAGGTAAAGGCCGGTGCGTTCCTCAAACTGGACCGCTCGCAACTGCCGAACTGGAAAAACCTCGA
CCCGAAACTGCTGGCCCTGCTTGAGAAAAACGATCCGGGCAACGAGCACTCGGTGCCGTACCTGTGGGGCACCAACGGCA
TCGGCTACAACGTCGACAAGGTCAAGCAAGTGCTGGGCATCGATCACATCGATTCCTGGGCCGTGCTGTTCGAACCGGAA
AACCTGAAGAAACTCACCCAGTGCGGTGTGTCGATGATGGACTCGGCCGATGAAGTGTTCCCGGCCATCCTCAACTACAT
GGGCATGGACCCGCGCAGCGAGAAACCCGAAGACTACAAGAAGGCCGAAGAGAAACTGCTTAGCATCCGGCCGTACATCA
CCTATTTCCATTCCTCGAAATACGTGTCCGACCTGGCCAACGGTGACATCTGCGTGGCCTTCGGTTACTCCGGCGACGTG
TTCCGGGCCGCCAATCGCGCCAAGGAAGCCAAGAACGGCGTGAACATCGCCTATTCGATTCCCAAGGAAGGCGCGAACCT
GTGGTTCGACCTGCTGGCGATTCCCGCCGATGCCGGCAACACCAAGGAAGCCCACGCCTTCATCAATTACCTGCTCGATC
CGCAAGTGATCGCCAAGGTCAGCGCCTCGGTGGGTTACGCCAACCCGAACCCGGCGGCCAAGCAATATATGGATCCCGAG
CTGGTCAACAATCCCGAGGTCTATCCGTCTCAGGAAGTCCTCGACAAGCTCTACATCTCCTCTACCCCGCCCCAGTCGAT
CATGCGTCTGATGACCCGGTCCTGGAGCAAAGTGAAGTCGAACAAATGA

Upstream 100 bases:

>100_bases
GAGCGTTTAAGCCTGAGACCTGATCGCTCCCGGACACTGTCCGGGAGCGATCCCTACCGACTTTACGAGCATAAAAAATC
CAACTCGAAGAGGTGTCGAT

Downstream 100 bases:

>100_bases
ATCAGTACACCCAGGAACACGCCCAGTCCTATTACGCCGCGTCGGCCCGGGCCAGCACACCCTATCCGGAACTGGCGGGC
GACCTGATCGCCGATGTCTG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRLLKSMIPAALALACSAGAFAQPQVSVYNWTDYIGETTLADFQSSSGIKVIYDVFDSNETLEGKLLAGRTGYDVVVPSN
HFLARQVKAGAFLKLDRSQLPNWKNLDPKLLALLEKNDPGNEHSVPYLWGTNGIGYNVDKVKQVLGIDHIDSWAVLFEPE
NLKKLTQCGVSMMDSADEVFPAILNYMGMDPRSEKPEDYKKAEEKLLSIRPYITYFHSSKYVSDLANGDICVAFGYSGDV
FRAANRAKEAKNGVNIAYSIPKEGANLWFDLLAIPADAGNTKEAHAFINYLLDPQVIAKVSASVGYANPNPAAKQYMDPE
LVNNPEVYPSQEVLDKLYISSTPPQSIMRLMTRSWSKVKSNK

Sequences:

>Translated_362_residues
MRLLKSMIPAALALACSAGAFAQPQVSVYNWTDYIGETTLADFQSSSGIKVIYDVFDSNETLEGKLLAGRTGYDVVVPSN
HFLARQVKAGAFLKLDRSQLPNWKNLDPKLLALLEKNDPGNEHSVPYLWGTNGIGYNVDKVKQVLGIDHIDSWAVLFEPE
NLKKLTQCGVSMMDSADEVFPAILNYMGMDPRSEKPEDYKKAEEKLLSIRPYITYFHSSKYVSDLANGDICVAFGYSGDV
FRAANRAKEAKNGVNIAYSIPKEGANLWFDLLAIPADAGNTKEAHAFINYLLDPQVIAKVSASVGYANPNPAAKQYMDPE
LVNNPEVYPSQEVLDKLYISSTPPQSIMRLMTRSWSKVKSNK
>Mature_362_residues
MRLLKSMIPAALALACSAGAFAQPQVSVYNWTDYIGETTLADFQSSSGIKVIYDVFDSNETLEGKLLAGRTGYDVVVPSN
HFLARQVKAGAFLKLDRSQLPNWKNLDPKLLALLEKNDPGNEHSVPYLWGTNGIGYNVDKVKQVLGIDHIDSWAVLFEPE
NLKKLTQCGVSMMDSADEVFPAILNYMGMDPRSEKPEDYKKAEEKLLSIRPYITYFHSSKYVSDLANGDICVAFGYSGDV
FRAANRAKEAKNGVNIAYSIPKEGANLWFDLLAIPADAGNTKEAHAFINYLLDPQVIAKVSASVGYANPNPAAKQYMDPE
LVNNPEVYPSQEVLDKLYISSTPPQSIMRLMTRSWSKVKSNK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=365, Percent_Identity=55.0684931506849, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1787367, Length=307, Percent_Identity=35.1791530944625, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787711, Length=301, Percent_Identity=24.5847176079734, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40077; Mature: 40077

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLKSMIPAALALACSAGAFAQPQVSVYNWTDYIGETTLADFQSSSGIKVIYDVFDSNE
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCHHHHHHCCCCCCEEEEEEECCCC
TLEGKLLAGRTGYDVVVPSNHFLARQVKAGAFLKLDRSQLPNWKNLDPKLLALLEKNDPG
CCCCEEEECCCCCEEEECCCCHHHHHHCCCCEEEECHHHCCCCCCCCHHHHHEEECCCCC
NEHSVPYLWGTNGIGYNVDKVKQVLGIDHIDSWAVLFEPENLKKLTQCGVSMMDSADEVF
CCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCHHHHCCHHHHH
PAILNYMGMDPRSEKPEDYKKAEEKLLSIRPYITYFHSSKYVSDLANGDICVAFGYSGDV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHEEECCCHHHHHHCCCCEEEEECCCCCH
FRAANRAKEAKNGVNIAYSIPKEGANLWFDLLAIPADAGNTKEAHAFINYLLDPQVIAKV
HHHHHHHHHHHCCCEEEEECCCCCCHHEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHH
SASVGYANPNPAAKQYMDPELVNNPEVYPSQEVLDKLYISSTPPQSIMRLMTRSWSKVKS
HHHCCCCCCCHHHHHCCCCHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
NK
CC
>Mature Secondary Structure
MRLLKSMIPAALALACSAGAFAQPQVSVYNWTDYIGETTLADFQSSSGIKVIYDVFDSNE
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCHHHHHHCCCCCCEEEEEEECCCC
TLEGKLLAGRTGYDVVVPSNHFLARQVKAGAFLKLDRSQLPNWKNLDPKLLALLEKNDPG
CCCCEEEECCCCCEEEECCCCHHHHHHCCCCEEEECHHHCCCCCCCCHHHHHEEECCCCC
NEHSVPYLWGTNGIGYNVDKVKQVLGIDHIDSWAVLFEPENLKKLTQCGVSMMDSADEVF
CCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCHHHHCCHHHHH
PAILNYMGMDPRSEKPEDYKKAEEKLLSIRPYITYFHSSKYVSDLANGDICVAFGYSGDV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHEEECCCHHHHHHCCCCEEEEECCCCCH
FRAANRAKEAKNGVNIAYSIPKEGANLWFDLLAIPADAGNTKEAHAFINYLLDPQVIAKV
HHHHHHHHHHHCCCEEEEECCCCCCHHEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHH
SASVGYANPNPAAKQYMDPELVNNPEVYPSQEVLDKLYISSTPPQSIMRLMTRSWSKVKS
HHHCCCCCCCHHHHHCCCCHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]