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The map label for this gene is fliY [H]

Identifier: 77459027

GI number: 77459027

Start: 3228537

End: 3229358

Strand: Reverse

Name: fliY [H]

Synonym: Pfl01_2802

Alternate gene names: 77459027

Gene position: 3229358-3228537 (Counterclockwise)

Preceding gene: 77459029

Following gene: 77459026

Centisome position: 50.16

GC content: 56.33

Gene sequence:

>822_bases
TTGAAGACCCCGACACATTTGACACTTGGTCTGGCGCTCGCCGTCAGTTGCTCTGCAGCATTCGCCGGGCCCACCCTTGA
TCGCGTGACGCAAAAAGGTGAGCTCACCGGTGTCCTGATGGAGAGTTACCCGCCGTTCTCTTTTCTCAATGACCAGAATC
AGCTCGACGGCTTCGATGTGGATGTCGCTAAAGCTGTGGCCGAGCGCCTTGGGGTCAAGCTGAAACTTGAAACTCCATCC
TGGGACGTGATTGCCGCGGGGCGCTGGAACGGTCGATACGACATCTGCGTGTGCTCGATGACACCGAGCAAGGCGCGGGC
CGAAGTGTTCGACTTCCCCGTGGAGTATTACCAGTCGCCGGCGGTGATCGTGGTCAATGCAAAGGACAAGGACATTGCCA
CTGGCAAGGACTTGTCCGGCAAAAAAGTCGGTGTGATCAGTGCGTCGACTTATGAGGCCTATCTGAACAAGGATCTGGTC
ATTGAAGGCGCCGAGGACAAACCGTTGAGCTATCCGTTCGACAATGTCCAGATCGCGCCTTACGACAATGAAACCGTTGC
CTTCCAGGATCTCGCACTGGGTACCGGCGTGCGCCTGGATGCCATGGTCACCAACCTGATCACCGCGCGTGAACGCATCG
CTCAGGATCCGCGCTTCAAGATTGCCGGAGATCCACTGTATGCCGAACCGAACGTCGTGGCGGTCGAAAAAGGCGATCCG
CAATGGAATGCGAAAGTCACCGAAGTCATCGCACAACTCAAGACTGACGGCACGCTGAGCAAAATATCCCGCAAGTGGAT
CGGTTCTGATATCAGCCAATGA

Upstream 100 bases:

>100_bases
TATAGCCCAAGCGCAGCTTTTTATTCCCAAAACGAAGTTCATATTAATTTTATAGTGCGTTTTAATCTAATCAGGTTTTT
CCTGATTGGAGCATTCGCAC

Downstream 100 bases:

>100_bases
CAGCCATTAACCCGCATCCGGCCAAAGCGGCTGTTACCCCGCAAAAGCAGCCGCCACTGACACGTCAGCTGTTCGGCTTT
CGCACGCGCCTGTATCTGAC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKTPTHLTLGLALAVSCSAAFAGPTLDRVTQKGELTGVLMESYPPFSFLNDQNQLDGFDVDVAKAVAERLGVKLKLETPS
WDVIAAGRWNGRYDICVCSMTPSKARAEVFDFPVEYYQSPAVIVVNAKDKDIATGKDLSGKKVGVISASTYEAYLNKDLV
IEGAEDKPLSYPFDNVQIAPYDNETVAFQDLALGTGVRLDAMVTNLITARERIAQDPRFKIAGDPLYAEPNVVAVEKGDP
QWNAKVTEVIAQLKTDGTLSKISRKWIGSDISQ

Sequences:

>Translated_273_residues
MKTPTHLTLGLALAVSCSAAFAGPTLDRVTQKGELTGVLMESYPPFSFLNDQNQLDGFDVDVAKAVAERLGVKLKLETPS
WDVIAAGRWNGRYDICVCSMTPSKARAEVFDFPVEYYQSPAVIVVNAKDKDIATGKDLSGKKVGVISASTYEAYLNKDLV
IEGAEDKPLSYPFDNVQIAPYDNETVAFQDLALGTGVRLDAMVTNLITARERIAQDPRFKIAGDPLYAEPNVVAVEKGDP
QWNAKVTEVIAQLKTDGTLSKISRKWIGSDISQ
>Mature_273_residues
MKTPTHLTLGLALAVSCSAAFAGPTLDRVTQKGELTGVLMESYPPFSFLNDQNQLDGFDVDVAKAVAERLGVKLKLETPS
WDVIAAGRWNGRYDICVCSMTPSKARAEVFDFPVEYYQSPAVIVVNAKDKDIATGKDLSGKKVGVISASTYEAYLNKDLV
IEGAEDKPLSYPFDNVQIAPYDNETVAFQDLALGTGVRLDAMVTNLITARERIAQDPRFKIAGDPLYAEPNVVAVEKGDP
QWNAKVTEVIAQLKTDGTLSKISRKWIGSDISQ

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=279, Percent_Identity=29.3906810035842, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787085, Length=231, Percent_Identity=26.4069264069264, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788648, Length=239, Percent_Identity=24.6861924686192, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788649, Length=269, Percent_Identity=24.907063197026, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787031, Length=264, Percent_Identity=27.6515151515151, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787088, Length=233, Percent_Identity=25.7510729613734, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786876, Length=265, Percent_Identity=28.3018867924528, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29708; Mature: 29708

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPTHLTLGLALAVSCSAAFAGPTLDRVTQKGELTGVLMESYPPFSFLNDQNQLDGFDV
CCCCCEEHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCHHCCCCCCCCCCCCH
DVAKAVAERLGVKLKLETPSWDVIAAGRWNGRYDICVCSMTPSKARAEVFDFPVEYYQSP
HHHHHHHHHCCEEEEEECCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHCCCHHHCCCC
AVIVVNAKDKDIATGKDLSGKKVGVISASTYEAYLNKDLVIEGAEDKPLSYPFDNVQIAP
EEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHHCCCCEEEECCCCCCCCCCCCCCEECC
YDNETVAFQDLALGTGVRLDAMVTNLITARERIAQDPRFKIAGDPLYAEPNVVAVEKGDP
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCCEEEEECCCC
QWNAKVTEVIAQLKTDGTLSKISRKWIGSDISQ
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKTPTHLTLGLALAVSCSAAFAGPTLDRVTQKGELTGVLMESYPPFSFLNDQNQLDGFDV
CCCCCEEHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCHHCCCCCCCCCCCCH
DVAKAVAERLGVKLKLETPSWDVIAAGRWNGRYDICVCSMTPSKARAEVFDFPVEYYQSP
HHHHHHHHHCCEEEEEECCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHCCCHHHCCCC
AVIVVNAKDKDIATGKDLSGKKVGVISASTYEAYLNKDLVIEGAEDKPLSYPFDNVQIAP
EEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHHCCCCEEEECCCCCCCCCCCCCCEECC
YDNETVAFQDLALGTGVRLDAMVTNLITARERIAQDPRFKIAGDPLYAEPNVVAVEKGDP
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCCEEEEECCCC
QWNAKVTEVIAQLKTDGTLSKISRKWIGSDISQ
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]