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The map label for this gene is prpB [C]

Identifier: 77458997

GI number: 77458997

Start: 3197134

End: 3198006

Strand: Reverse

Name: prpB [C]

Synonym: Pfl01_2772

Alternate gene names: 77458997

Gene position: 3198006-3197134 (Counterclockwise)

Preceding gene: 77458998

Following gene: 77458996

Centisome position: 49.67

GC content: 52.46

Gene sequence:

>873_bases
ATGATAGAGCGATCACATCATGATTTACGCAAGGTGTTTGGTGAGTTACTCCAGGGAGACACCTGCAAATTTGCAGCTTC
CGTATTCGATCCGATCTCAGTTCGGATGGCATACGACCTTGGCTTTGACGTTGCCATTCAAGGCGGCTCCGTTGCGTCGC
TTCAGGTGCTCGGCGCACCTGACATTGCATTACTCACGCTGGATGAATATGTCGAGCAGGTTTCCAGGGTCGGACGGGCA
AGCCAGATTCCGATCATTGGGGATGCTGACCATGGCTTTGGTAACGCGCTGAATGTGATGCGAACAGTCACAGAGTTACA
GAAGGCCGGCGTTGCAGCCCTGACACTGGAAGACACCCATCTACCGGCCAAGTACGACGAGCAGTCGCATGTGCTTATTG
AAAGGGAAGAAGCCGCAGCCAAAATCTATGCCGCGCGATTTGCCCGGTCGGATGATGCACTCAGCATCATCGCCCGTACG
AATGTGGCTGTGACCACTTTGGACGACTCCATCGCTCGCACTGCGGCTTATCAGAAGGCTGGAGCAGATGCTATTTGCCT
GGTCGGTGTTAAGGACTTCCAGCACCTAGAAGCACTTACGGCACATCTCAGAGTGCCGATCATGCTGATCAACTATGGAA
ATCCTGCTCTGAGCAATGTTGAAAAATTGAGTGCTGCCAATGTGCGGATCGTGGTCAATGGGCATGCCCCCTATTTGTCC
GCAATCAAAGCAACCTACGAGGCGCTACGCGAACAAAGCGGCACGGAGGGGAGCGAGCTATCCCTTCCTGAGCTGCTTTC
TAAATACACACTCTCGGACAGTTATCGTGAGTGGGCAAAAACCTTTTTGAAGTCAGAGCACGATTCAAATTGA

Upstream 100 bases:

>100_bases
AGCGATCCGGTCATTCCCATCTAAACGGTTATGACCGGTAGTGTTTTCCATGCCTTCTTCCGCGTGGAGAGACCTGATTG
GAAAGATTGATGGAGCCAAC

Downstream 100 bases:

>100_bases
TATTGGAACAGGTGGAATACGCCGTCATGAAAAAGACAATCTTGGCCTTCAGCCGGGTCTCCCCCAGCCTGCTTGAACCT
TACAAAGACGACTACGAAGT

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MIERSHHDLRKVFGELLQGDTCKFAASVFDPISVRMAYDLGFDVAIQGGSVASLQVLGAPDIALLTLDEYVEQVSRVGRA
SQIPIIGDADHGFGNALNVMRTVTELQKAGVAALTLEDTHLPAKYDEQSHVLIEREEAAAKIYAARFARSDDALSIIART
NVAVTTLDDSIARTAAYQKAGADAICLVGVKDFQHLEALTAHLRVPIMLINYGNPALSNVEKLSAANVRIVVNGHAPYLS
AIKATYEALREQSGTEGSELSLPELLSKYTLSDSYREWAKTFLKSEHDSN

Sequences:

>Translated_290_residues
MIERSHHDLRKVFGELLQGDTCKFAASVFDPISVRMAYDLGFDVAIQGGSVASLQVLGAPDIALLTLDEYVEQVSRVGRA
SQIPIIGDADHGFGNALNVMRTVTELQKAGVAALTLEDTHLPAKYDEQSHVLIEREEAAAKIYAARFARSDDALSIIART
NVAVTTLDDSIARTAAYQKAGADAICLVGVKDFQHLEALTAHLRVPIMLINYGNPALSNVEKLSAANVRIVVNGHAPYLS
AIKATYEALREQSGTEGSELSLPELLSKYTLSDSYREWAKTFLKSEHDSN
>Mature_290_residues
MIERSHHDLRKVFGELLQGDTCKFAASVFDPISVRMAYDLGFDVAIQGGSVASLQVLGAPDIALLTLDEYVEQVSRVGRA
SQIPIIGDADHGFGNALNVMRTVTELQKAGVAALTLEDTHLPAKYDEQSHVLIEREEAAAKIYAARFARSDDALSIIART
NVAVTTLDDSIARTAAYQKAGADAICLVGVKDFQHLEALTAHLRVPIMLINYGNPALSNVEKLSAANVRIVVNGHAPYLS
AIKATYEALREQSGTEGSELSLPELLSKYTLSDSYREWAKTFLKSEHDSN

Specific function: Catalyzes the decarboxylation of oxaloacetate into pyruvate. Seems to play a role in maintaining cellular concentrations of bicarbonate and pyruvate

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Oxaloacetate decarboxylase family

Homologues:

Organism=Escherichia coli, GI1786525, Length=249, Percent_Identity=30.1204819277108, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OADC2_PSEPF (Q3KCJ2)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_348503.1
- STRING:   Q3KCJ2
- GeneID:   3714513
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_2772
- eggNOG:   COG2513
- HOGENOM:   HBG728656
- OMA:   MIERSHH
- ProtClustDB:   CLSK868955
- BioCyc:   PFLU205922:PFL_2772-MONOMER
- HAMAP:   MF_01299
- InterPro:   IPR000918
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60

Pfam domain/function: PF00463 ICL; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.1.3

Molecular weight: Translated: 31485; Mature: 31485

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 159-159 BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERSHHDLRKVFGELLQGDTCKFAASVFDPISVRMAYDLGFDVAIQGGSVASLQVLGAP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCEEEEECCCCEEEEEEECCC
DIALLTLDEYVEQVSRVGRASQIPIIGDADHGFGNALNVMRTVTELQKAGVAALTLEDTH
CEEEEEHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCC
LPAKYDEQSHVLIEREEAAAKIYAARFARSDDALSIIARTNVAVTTLDDSIARTAAYQKA
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHC
GADAICLVGVKDFQHLEALTAHLRVPIMLINYGNPALSNVEKLSAANVRIVVNGHAPYLS
CCCEEEEEECHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCHHHH
AIKATYEALREQSGTEGSELSLPELLSKYTLSDSYREWAKTFLKSEHDSN
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIERSHHDLRKVFGELLQGDTCKFAASVFDPISVRMAYDLGFDVAIQGGSVASLQVLGAP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCEEEEECCCCEEEEEEECCC
DIALLTLDEYVEQVSRVGRASQIPIIGDADHGFGNALNVMRTVTELQKAGVAALTLEDTH
CEEEEEHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCC
LPAKYDEQSHVLIEREEAAAKIYAARFARSDDALSIIARTNVAVTTLDDSIARTAAYQKA
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHC
GADAICLVGVKDFQHLEALTAHLRVPIMLINYGNPALSNVEKLSAANVRIVVNGHAPYLS
CCCEEEEEECHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCHHHH
AIKATYEALREQSGTEGSELSLPELLSKYTLSDSYREWAKTFLKSEHDSN
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA