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The map label for this gene is ghrB [H]

Identifier: 77458996

GI number: 77458996

Start: 3196142

End: 3197107

Strand: Reverse

Name: ghrB [H]

Synonym: Pfl01_2771

Alternate gene names: 77458996

Gene position: 3197107-3196142 (Counterclockwise)

Preceding gene: 77458997

Following gene: 77458994

Centisome position: 49.66

GC content: 52.38

Gene sequence:

>966_bases
ATGAAAAAGACAATCTTGGCCTTCAGCCGGGTCTCCCCCAGCCTGCTTGAACCTTACAAAGACGACTACGAAGTCATCAT
CATTAGTCCTGAGCTTGGTGATATGGACGCCCAGTTCGAGGCCGCTATTTCGAACGCTCACGGGCTGATTGGTGGAAGTC
GTCGTCTGGGTGAAGCTGAGCTGGCTGCGGCTGTAAATCTTGAGATCGTCTCAAGCATATCGGTTGGCTACGACAACTAT
GACCTCGACTACCTCAACCGCCGAGGGATCATGTTGACGAATACGCCAGATGTGTTGAACGAAACCACTGCTGACCTGGC
TTTGGCCCTGATCCTCGCCACAGCAAGACGCATTCCTGAGCTTGATTCCTGGACTAAAGAGGGTAACTGGACACAAACCA
TCGATGCATCCCAATTTGGGTGCGACGTTCATGGAAAGACGTTGGGCATTATCGGTCTGGGAAAAATCGGAGAAGCCATC
GCACGCAGAGGCCGCTTTGGTTTTGGCATGACCATTTTGTATAGCGGCAACAGTCGTAAACCAAAGCTTGAACAGGAACT
TGGTGCGAGGTTTGTACCGCAGGAAGAGCTTTTAAGCGCGTCTGACTTTGTTTGTCCGGTCGTGCCATTGACGGACGCCA
CGAGGAATCTTATCGGACGCAAAGAACTCGCTTTGATGGGCCCGGAAAGTATTTTGATCAATATCTCGCGTGGGCCTGTC
GTGGATCAGGACGCACTCATCGAGGCTCTGCAAGAAAAGACAATCCGGGCGGCTGGTTTGGACGTTTATGTGAAGGAGCC
CCTGACGAACTCCGAGCTTTTTAACCTGAGAAACGTCGTCACCGTACCTCACATTGGCTCTGCGACGACTGACACCCGCA
ACGCAATGGCCAAGCGAGCCCTGGAAAATTTGCTTGCGGGTCTGGAAGGTCGCCAGCCGAGAGATCTTGTGCACTTCAAG
AAGTAA

Upstream 100 bases:

>100_bases
CTAAATACACACTCTCGGACAGTTATCGTGAGTGGGCAAAAACCTTTTTGAAGTCAGAGCACGATTCAAATTGATATTGG
AACAGGTGGAATACGCCGTC

Downstream 100 bases:

>100_bases
GAAGAAGTAGGGCGGATTGAGCTCAAAATCGCCGGCAATATGGCTGCTCTCCGGGTGACGTACATAGTTGGGGTGGCAAG
CGTTTTCTGACGCAAGCCAG

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKKTILAFSRVSPSLLEPYKDDYEVIIISPELGDMDAQFEAAISNAHGLIGGSRRLGEAELAAAVNLEIVSSISVGYDNY
DLDYLNRRGIMLTNTPDVLNETTADLALALILATARRIPELDSWTKEGNWTQTIDASQFGCDVHGKTLGIIGLGKIGEAI
ARRGRFGFGMTILYSGNSRKPKLEQELGARFVPQEELLSASDFVCPVVPLTDATRNLIGRKELALMGPESILINISRGPV
VDQDALIEALQEKTIRAAGLDVYVKEPLTNSELFNLRNVVTVPHIGSATTDTRNAMAKRALENLLAGLEGRQPRDLVHFK
K

Sequences:

>Translated_321_residues
MKKTILAFSRVSPSLLEPYKDDYEVIIISPELGDMDAQFEAAISNAHGLIGGSRRLGEAELAAAVNLEIVSSISVGYDNY
DLDYLNRRGIMLTNTPDVLNETTADLALALILATARRIPELDSWTKEGNWTQTIDASQFGCDVHGKTLGIIGLGKIGEAI
ARRGRFGFGMTILYSGNSRKPKLEQELGARFVPQEELLSASDFVCPVVPLTDATRNLIGRKELALMGPESILINISRGPV
VDQDALIEALQEKTIRAAGLDVYVKEPLTNSELFNLRNVVTVPHIGSATTDTRNAMAKRALENLLAGLEGRQPRDLVHFK
K
>Mature_321_residues
MKKTILAFSRVSPSLLEPYKDDYEVIIISPELGDMDAQFEAAISNAHGLIGGSRRLGEAELAAAVNLEIVSSISVGYDNY
DLDYLNRRGIMLTNTPDVLNETTADLALALILATARRIPELDSWTKEGNWTQTIDASQFGCDVHGKTLGIIGLGKIGEAI
ARRGRFGFGMTILYSGNSRKPKLEQELGARFVPQEELLSASDFVCPVVPLTDATRNLIGRKELALMGPESILINISRGPV
VDQDALIEALQEKTIRAAGLDVYVKEPLTNSELFNLRNVVTVPHIGSATTDTRNAMAKRALENLLAGLEGRQPRDLVHFK
K

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=280, Percent_Identity=44.6428571428571, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI23308577, Length=232, Percent_Identity=33.1896551724138, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI61743967, Length=257, Percent_Identity=32.295719844358, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4557497, Length=257, Percent_Identity=32.295719844358, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI145580578, Length=257, Percent_Identity=30.3501945525292, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4557499, Length=257, Percent_Identity=30.3501945525292, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI145580575, Length=257, Percent_Identity=30.3501945525292, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87082289, Length=321, Percent_Identity=43.9252336448598, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789279, Length=265, Percent_Identity=34.7169811320755, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787645, Length=245, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17532191, Length=245, Percent_Identity=30.2040816326531, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=283, Percent_Identity=27.208480565371, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324055, Length=270, Percent_Identity=34.4444444444444, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6320925, Length=269, Percent_Identity=33.0855018587361, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=308, Percent_Identity=31.4935064935065, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321253, Length=306, Percent_Identity=30.3921568627451, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6325144, Length=166, Percent_Identity=30.1204819277108, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574286, Length=320, Percent_Identity=37.1875, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI45552429, Length=266, Percent_Identity=40.6015037593985, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI28574284, Length=266, Percent_Identity=40.6015037593985, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24585514, Length=266, Percent_Identity=40.6015037593985, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI28574282, Length=266, Percent_Identity=40.6015037593985, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45551003, Length=266, Percent_Identity=40.6015037593985, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI28571528, Length=250, Percent_Identity=42, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24585516, Length=251, Percent_Identity=35.8565737051793, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19921140, Length=313, Percent_Identity=27.7955271565495, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24646446, Length=225, Percent_Identity=32.4444444444444, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24646448, Length=225, Percent_Identity=32.4444444444444, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24646452, Length=225, Percent_Identity=32.4444444444444, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24646450, Length=225, Percent_Identity=32.4444444444444, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI62472511, Length=225, Percent_Identity=32.4444444444444, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35044; Mature: 35044

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTILAFSRVSPSLLEPYKDDYEVIIISPELGDMDAQFEAAISNAHGLIGGSRRLGEAE
CCCCEEEHHHCCHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHH
LAAAVNLEIVSSISVGYDNYDLDYLNRRGIMLTNTPDVLNETTADLALALILATARRIPE
HHHHHHHEEEEHEECCCCCCCHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
LDSWTKEGNWTQTIDASQFGCDVHGKTLGIIGLGKIGEAIARRGRFGFGMTILYSGNSRK
CCCCCCCCCEEEEECCHHCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCEEEEECCCCCC
PKLEQELGARFVPQEELLSASDFVCPVVPLTDATRNLIGRKELALMGPESILINISRGPV
CCHHHHHCCCCCCHHHHHCCCCCEEEEEECCHHHHHHHCCHHHEEECCCEEEEECCCCCC
VDQDALIEALQEKTIRAAGLDVYVKEPLTNSELFNLRNVVTVPHIGSATTDTRNAMAKRA
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCEEECCCCCCCCCHHHHHHHHHH
LENLLAGLEGRQPRDLVHFKK
HHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKTILAFSRVSPSLLEPYKDDYEVIIISPELGDMDAQFEAAISNAHGLIGGSRRLGEAE
CCCCEEEHHHCCHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHH
LAAAVNLEIVSSISVGYDNYDLDYLNRRGIMLTNTPDVLNETTADLALALILATARRIPE
HHHHHHHEEEEHEECCCCCCCHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
LDSWTKEGNWTQTIDASQFGCDVHGKTLGIIGLGKIGEAIARRGRFGFGMTILYSGNSRK
CCCCCCCCCEEEEECCHHCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCEEEEECCCCCC
PKLEQELGARFVPQEELLSASDFVCPVVPLTDATRNLIGRKELALMGPESILINISRGPV
CCHHHHHCCCCCCHHHHHCCCCCEEEEEECCHHHHHHHCCHHHEEECCCEEEEECCCCCC
VDQDALIEALQEKTIRAAGLDVYVKEPLTNSELFNLRNVVTVPHIGSATTDTRNAMAKRA
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCEEECCCCCCCCCHHHHHHHHHH
LENLLAGLEGRQPRDLVHFKK
HHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA