Click here to switch to the map view.

The map label for this gene is tktB [C]

Identifier: 77458944

GI number: 77458944

Start: 3135829

End: 3136677

Strand: Reverse

Name: tktB [C]

Synonym: Pfl01_2719

Alternate gene names: 77458944

Gene position: 3136677-3135829 (Counterclockwise)

Preceding gene: 77458945

Following gene: 77458943

Centisome position: 48.72

GC content: 62.07

Gene sequence:

>849_bases
ATGACTGCCAACCTTTCATTCACAACACCCCAGACGCTGGCCGAGCGCGCCCACAACATCCGCCGCCATGCCTTGCGCAT
GGGCCAGGTGCAAGGCCAGGGCTACGTCGGCCAGGCCCTCGGTGCGGCGGACCTGCTCGCGGTCGCCTACTTCCATGCGC
TGAGTTATCGTCCCGAAGACCCGGCGTGGGAGCCGCGTGATCGTTTCTATCTGTCGATCGGTCACTATGCGATTGCGCTG
TACGCGGCGCTGATCGAAGCCGAGATCATCCCGCTCGATGAGCTTGAAACCTACGGCTCGGATGACAGCCGATTGCCGAT
GTCGGGCATGGCCGCCTACACGCCGGGCATGGAAATCACCGGTGGCTCGCTGGGTCAGGGGCTGGGCATCGCGGTCGGCG
CCTGCCTGGGCCTCAAGCGCAAGGGCTCGACATCGTTCGTCTACAACCTGCTGTCGGACGGCGAGCTGAACGAAGGCTCG
ACCTGGGAAGCCGTGATGTCGGCCTCGCACTGGAAGCTCGACAACCTGATCGCCATCGTCGACGTCAACAATCAGCAAGC
GGACGGCCATTCCAGCGAGATCCTGTCGTTCGAACCCATCGTGGATCGCTGGAAAGCGTTTGGCTGGTTCACCCAGCGCG
TCGACGGCAACGACCTGGACGCACTGGTCGAAGCCTTCGATGCCGCCCGCCACCACCCCGGCACTCAGCCACGGGTGATC
ATCTGCGACACCACCATGGGCAAAGGTGTGCCCTTCCTCGAAAACCGCGAAAAGACCCATTTCATCCGTGTGGACGAACA
CGAATGGGATCTGGCACTGACCACGCTTGAAGAAGGAAAAAACCAATGA

Upstream 100 bases:

>100_bases
CGGCATGTACCTGATCACCGGCGTATTGCTGATCGGCGCCTCGCTGGTCCTGCGCATTCCCGCTGCCTTGGTCAATCGTT
GATTCACAGGAGAATTCACC

Downstream 100 bases:

>100_bases
GCAACGCCGCCAACACACCGACCTCGACTGGTGAACCGATCAAGAAGCGCCTGACCACCTCGGCGATGATTGCCTCGATT
GCCTCTGAAGGCCAAGCCAC

Product: transketolase subunit A

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTANLSFTTPQTLAERAHNIRRHALRMGQVQGQGYVGQALGAADLLAVAYFHALSYRPEDPAWEPRDRFYLSIGHYAIAL
YAALIEAEIIPLDELETYGSDDSRLPMSGMAAYTPGMEITGGSLGQGLGIAVGACLGLKRKGSTSFVYNLLSDGELNEGS
TWEAVMSASHWKLDNLIAIVDVNNQQADGHSSEILSFEPIVDRWKAFGWFTQRVDGNDLDALVEAFDAARHHPGTQPRVI
ICDTTMGKGVPFLENREKTHFIRVDEHEWDLALTTLEEGKNQ

Sequences:

>Translated_282_residues
MTANLSFTTPQTLAERAHNIRRHALRMGQVQGQGYVGQALGAADLLAVAYFHALSYRPEDPAWEPRDRFYLSIGHYAIAL
YAALIEAEIIPLDELETYGSDDSRLPMSGMAAYTPGMEITGGSLGQGLGIAVGACLGLKRKGSTSFVYNLLSDGELNEGS
TWEAVMSASHWKLDNLIAIVDVNNQQADGHSSEILSFEPIVDRWKAFGWFTQRVDGNDLDALVEAFDAARHHPGTQPRVI
ICDTTMGKGVPFLENREKTHFIRVDEHEWDLALTTLEEGKNQ
>Mature_281_residues
TANLSFTTPQTLAERAHNIRRHALRMGQVQGQGYVGQALGAADLLAVAYFHALSYRPEDPAWEPRDRFYLSIGHYAIALY
AALIEAEIIPLDELETYGSDDSRLPMSGMAAYTPGMEITGGSLGQGLGIAVGACLGLKRKGSTSFVYNLLSDGELNEGST
WEAVMSASHWKLDNLIAIVDVNNQQADGHSSEILSFEPIVDRWKAFGWFTQRVDGNDLDALVEAFDAARHHPGTQPRVII
CDTTMGKGVPFLENREKTHFIRVDEHEWDLALTTLEEGKNQ

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=278, Percent_Identity=36.3309352517986, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI205277463, Length=262, Percent_Identity=38.1679389312977, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI4507521, Length=262, Percent_Identity=38.1679389312977, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI225637459, Length=277, Percent_Identity=29.6028880866426, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI225637461, Length=277, Percent_Identity=29.2418772563177, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI225637463, Length=240, Percent_Identity=30.8333333333333, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1788808, Length=272, Percent_Identity=32.3529411764706, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI48994911, Length=258, Percent_Identity=32.1705426356589, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17539652, Length=252, Percent_Identity=34.9206349206349, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6325331, Length=245, Percent_Identity=34.2857142857143, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319593, Length=241, Percent_Identity=31.9502074688797, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45551847, Length=256, Percent_Identity=33.203125, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45550715, Length=256, Percent_Identity=33.203125, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24666278, Length=262, Percent_Identity=30.5343511450382, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24645119, Length=219, Percent_Identity=34.703196347032, Blast_Score=104, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1

Molecular weight: Translated: 31078; Mature: 30947

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANLSFTTPQTLAERAHNIRRHALRMGQVQGQGYVGQALGAADLLAVAYFHALSYRPED
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PAWEPRDRFYLSIGHYAIALYAALIEAEIIPLDELETYGSDDSRLPMSGMAAYTPGMEIT
CCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCEEECCCCEEC
GGSLGQGLGIAVGACLGLKRKGSTSFVYNLLSDGELNEGSTWEAVMSASHWKLDNLIAIV
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEE
DVNNQQADGHSSEILSFEPIVDRWKAFGWFTQRVDGNDLDALVEAFDAARHHPGTQPRVI
ECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
ICDTTMGKGVPFLENREKTHFIRVDEHEWDLALTTLEEGKNQ
EEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEHHCCCCC
>Mature Secondary Structure 
TANLSFTTPQTLAERAHNIRRHALRMGQVQGQGYVGQALGAADLLAVAYFHALSYRPED
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PAWEPRDRFYLSIGHYAIALYAALIEAEIIPLDELETYGSDDSRLPMSGMAAYTPGMEIT
CCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCEEECCCCEEC
GGSLGQGLGIAVGACLGLKRKGSTSFVYNLLSDGELNEGSTWEAVMSASHWKLDNLIAIV
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEE
DVNNQQADGHSSEILSFEPIVDRWKAFGWFTQRVDGNDLDALVEAFDAARHHPGTQPRVI
ECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
ICDTTMGKGVPFLENREKTHFIRVDEHEWDLALTTLEEGKNQ
EEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]