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The map label for this gene is bepF [H]

Identifier: 77458884

GI number: 77458884

Start: 3051644

End: 3052897

Strand: Reverse

Name: bepF [H]

Synonym: Pfl01_2659

Alternate gene names: 77458884

Gene position: 3052897-3051644 (Counterclockwise)

Preceding gene: 77458890

Following gene: 77458883

Centisome position: 47.42

GC content: 63.4

Gene sequence:

>1254_bases
ATGGAACAATCACTCAAACATTTGCGCTTCCCGTTGGCTCTGTTGGCCGTGCTGGTGATGAGCGCGTGCGGCAAGACTCC
GGAAACTGCCGCCACCATGCCCGCTGCCAAAGTCAGTGTGGCCAAGGTGCTGGAACAACCGGTCAACGAGTGGGACGAAT
TCACCGGGCGCCTCGAGGCGCCGGAAACCGTTGAAATCCGTCCGCGGGTCTCCGGCCAGATCGACGAAGTCGCCTTCACC
GAAGGTGCGCTGGTCAAGAAAGGCGACCTGCTGTTCCAGATCGACCCGCGTCCGTTCCAGGCTGAGGTCCGCCGCCTCGA
AGCTCTGGTTGCCCAGGCCCGCGCCAACGCCACCCGCAGTGAAAACGAAGCCGGCCGAGGCGAACGCCTGCGTGCCAGCA
ACGCCATCTCCGCCGAACTGGCCGACTCGCGCACCAGCGCTGCACAGGAAGCACGCGCCGCTGTCGGCGCCCTGCAAGCG
CAACTGGATCTGGCCAAACTCAACCTGAGCTTCACCCGCGTCACCGCGCCAATCAGCGGCCGCGTCAGCCGTGCCGAGAT
CACCGCCGGTAACCTGGTGACTGCCGATACCACCCCGCTGACCAGCGTGGTGTCCACCGACAAGGTCTACGCCTACTTCG
ACGCCGACGAGCGTGTATTCCTCAAATACACCCAACTCGCCCGTCAGGGTCAGCGCGGCGCGACCACCCCGGTGTACATG
GGCCTGTCCAATGAAGACGGCAACCCGCACCTGGGCCAGATGAACTTTATCGACAACCAGGTCAACCCGAAGACCGGCAC
CATCCGTGGTCGCGCGGTGTTCGACAACAGCGACGGCAGCTACACCCCGGGTCTGTACGCACGCCTGAAACTGGTCGGCA
GCGGCACCTACAACGCCATGCTGATCAACGACGAAGCGGTCGGTACCGACCTCGGCAAGAAGTTCGTGCTGGTGATGGAT
GGCGACAACAAGACCGCCTACCGCGCCGTTGAACTCGGTCCGAAGATCGAAGGGCTGCGTATCGTGCGCAGCGGCCTGAA
CAAGGACGACACGATCGTCGTCAAGGGTCTGCAACGGGTTCGCCCTGGCTCCCCGGTTTCGCCTGAAGTGATCCCGATGG
CCAGCGAGCAAACCATTGCCGCTCTCGCTCAACAACGACAAGCGCTGGAAGCCAGCAACCTGCCTAAAGTCGCACCTGCC
AAAGGCGCGCCGGGTTCGGTTGTGAAACTGGCTGCTGCGACCCCACGCGGTTAA

Upstream 100 bases:

>100_bases
TATCATTCCGAATGATAGTTACCTTCGTGTCATTCGATTCGTTTGCTACCTACCCGCCGAGCATCATCCGCCCATCTTCA
ATACATTGGCGGATGCATCC

Downstream 100 bases:

>100_bases
GGGACGACAACTCCGATGAATTTTTCCCAATTCTTCATTTCACGGCCGATCTTCGCAGCGGTGCTTTCGCTGTTGATCCT
GATCGCCGGTGCGATTTCGC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MEQSLKHLRFPLALLAVLVMSACGKTPETAATMPAAKVSVAKVLEQPVNEWDEFTGRLEAPETVEIRPRVSGQIDEVAFT
EGALVKKGDLLFQIDPRPFQAEVRRLEALVAQARANATRSENEAGRGERLRASNAISAELADSRTSAAQEARAAVGALQA
QLDLAKLNLSFTRVTAPISGRVSRAEITAGNLVTADTTPLTSVVSTDKVYAYFDADERVFLKYTQLARQGQRGATTPVYM
GLSNEDGNPHLGQMNFIDNQVNPKTGTIRGRAVFDNSDGSYTPGLYARLKLVGSGTYNAMLINDEAVGTDLGKKFVLVMD
GDNKTAYRAVELGPKIEGLRIVRSGLNKDDTIVVKGLQRVRPGSPVSPEVIPMASEQTIAALAQQRQALEASNLPKVAPA
KGAPGSVVKLAAATPRG

Sequences:

>Translated_417_residues
MEQSLKHLRFPLALLAVLVMSACGKTPETAATMPAAKVSVAKVLEQPVNEWDEFTGRLEAPETVEIRPRVSGQIDEVAFT
EGALVKKGDLLFQIDPRPFQAEVRRLEALVAQARANATRSENEAGRGERLRASNAISAELADSRTSAAQEARAAVGALQA
QLDLAKLNLSFTRVTAPISGRVSRAEITAGNLVTADTTPLTSVVSTDKVYAYFDADERVFLKYTQLARQGQRGATTPVYM
GLSNEDGNPHLGQMNFIDNQVNPKTGTIRGRAVFDNSDGSYTPGLYARLKLVGSGTYNAMLINDEAVGTDLGKKFVLVMD
GDNKTAYRAVELGPKIEGLRIVRSGLNKDDTIVVKGLQRVRPGSPVSPEVIPMASEQTIAALAQQRQALEASNLPKVAPA
KGAPGSVVKLAAATPRG
>Mature_417_residues
MEQSLKHLRFPLALLAVLVMSACGKTPETAATMPAAKVSVAKVLEQPVNEWDEFTGRLEAPETVEIRPRVSGQIDEVAFT
EGALVKKGDLLFQIDPRPFQAEVRRLEALVAQARANATRSENEAGRGERLRASNAISAELADSRTSAAQEARAAVGALQA
QLDLAKLNLSFTRVTAPISGRVSRAEITAGNLVTADTTPLTSVVSTDKVYAYFDADERVFLKYTQLARQGQRGATTPVYM
GLSNEDGNPHLGQMNFIDNQVNPKTGTIRGRAVFDNSDGSYTPGLYARLKLVGSGTYNAMLINDEAVGTDLGKKFVLVMD
GDNKTAYRAVELGPKIEGLRIVRSGLNKDDTIVVKGLQRVRPGSPVSPEVIPMASEQTIAALAQQRQALEASNLPKVAPA
KGAPGSVVKLAAATPRG

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=364, Percent_Identity=32.967032967033, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789665, Length=370, Percent_Identity=32.4324324324324, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1786668, Length=384, Percent_Identity=32.5520833333333, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI87082036, Length=348, Percent_Identity=27.8735632183908, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87081951, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081796, Length=155, Percent_Identity=31.6129032258064, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790519, Length=257, Percent_Identity=27.2373540856031, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789637, Length=201, Percent_Identity=31.8407960199005, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44577; Mature: 44577

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSLKHLRFPLALLAVLVMSACGKTPETAATMPAAKVSVAKVLEQPVNEWDEFTGRLEA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PETVEIRPRVSGQIDEVAFTEGALVKKGDLLFQIDPRPFQAEVRRLEALVAQARANATRS
CCEEEECCCCCCCCCCEEECCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
ENEAGRGERLRASNAISAELADSRTSAAQEARAAVGALQAQLDLAKLNLSFTRVTAPISG
CCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCC
RVSRAEITAGNLVTADTTPLTSVVSTDKVYAYFDADERVFLKYTQLARQGQRGATTPVYM
CCEEEEEECCCEEECCCCCHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCCCCCCCEEEE
GLSNEDGNPHLGQMNFIDNQVNPKTGTIRGRAVFDNSDGSYTPGLYARLKLVGSGTYNAM
EECCCCCCCCCCEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCCEEEE
LINDEAVGTDLGKKFVLVMDGDNKTAYRAVELGPKIEGLRIVRSGLNKDDTIVVKGLQRV
EEECHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEECHHHC
RPGSPVSPEVIPMASEQTIAALAQQRQALEASNLPKVAPAKGAPGSVVKLAAATPRG
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MEQSLKHLRFPLALLAVLVMSACGKTPETAATMPAAKVSVAKVLEQPVNEWDEFTGRLEA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PETVEIRPRVSGQIDEVAFTEGALVKKGDLLFQIDPRPFQAEVRRLEALVAQARANATRS
CCEEEECCCCCCCCCCEEECCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
ENEAGRGERLRASNAISAELADSRTSAAQEARAAVGALQAQLDLAKLNLSFTRVTAPISG
CCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCC
RVSRAEITAGNLVTADTTPLTSVVSTDKVYAYFDADERVFLKYTQLARQGQRGATTPVYM
CCEEEEEECCCEEECCCCCHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCCCCCCCEEEE
GLSNEDGNPHLGQMNFIDNQVNPKTGTIRGRAVFDNSDGSYTPGLYARLKLVGSGTYNAM
EECCCCCCCCCCEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCCEEEE
LINDEAVGTDLGKKFVLVMDGDNKTAYRAVELGPKIEGLRIVRSGLNKDDTIVVKGLQRV
EEECHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEECHHHC
RPGSPVSPEVIPMASEQTIAALAQQRQALEASNLPKVAPAKGAPGSVVKLAAATPRG
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]