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The map label for this gene is leuC1 [H]

Identifier: 77458587

GI number: 77458587

Start: 2717856

End: 2719274

Strand: Reverse

Name: leuC1 [H]

Synonym: Pfl01_2360

Alternate gene names: 77458587

Gene position: 2719274-2717856 (Counterclockwise)

Preceding gene: 77458588

Following gene: 77458586

Centisome position: 42.24

GC content: 65.12

Gene sequence:

>1419_bases
ATGCCCAGCCCGAGAACGCTGTACCAGAAGCACATCGACAGTCACACCGTCTGCACCCTCGACGATCAGGGCCACGTGCT
GCTTTACATCGACCGCCAGGTCGCCAACGAATACACCAGCCCGCAAGCCTTCAGCGGCCTGCGCGAGGCCGGGCGCACGG
TGTGGCGGCCTGCGGCGACGCTGGCGGTGGTCGATCATGTGAACCCCACCGCACCGAAACGCATCGCGACCATGCCCGAC
GCCGGGGGCGCGCGGCAGGTGTCGTACTTCGAAGAAAACTGCCGCGACTTCGGCATCGAGCTGTTCGATGTGCTGGACAA
GCGCCAGGGCATCGAACACGTCGTAGCGCCGGAACAGGGTTTCATTTTGCCGGGCATGGTGGTCGCGGCCGGTGACAGCC
ACACCACCACTTACGGCGCACTGGGAGCGTTCGGTTTCGGCATCGGCACCTCGGAAATCGAGCACCTGCTGGCAACCCAG
ACGCTGGTCTACAAACGTCTGAAAACCCTGCGGGTGACGGTCAACGGCGAACTGGGCGCAGGCGTCACCTCCAAGGACAT
CATCATGGCGCTGATCGAAAAGATCGGCGCGTCCGGCGCCACCGGTTACGCCATCGAGTTCACCGGCCCGGCCATCAGCG
CCCTCAGCGTCGAAGCGCGCATGACCATCTGCAACATGGCCGTCGAGGCCGGCGCCCGGGGTGCGTTCATGGCACCGGAT
GACAAGGTGTTCGCCTACCTGCAACACAAGCCGCGTGCGCCCAAGGGCAAGCTCTGGGAGCAGGCCATCGATCACTGGAA
AACCCTGCACAGCGATGAGGGCGCAGTGTTCGATCGCGAAGTCATCCTTGATGTCGCCGAGCTGGAACCAATGGTGACCT
GGGGCACCAGCCCCGATCAGGCCGCGCCGATCAAGGCACACGTGCCGGATCCGGCCGCCCAGCCCGATCCGATCCTGCGT
CAGGGCCTGCAGCGCGCCCTCGACTACATGGGGCTGACGCCCGGCATGCCGCTGAACGAAGTGACGATCAGCCATGCCTT
CATCGGTTCCTGCACCAATGCGCGGATCGAAGATTTGCGTGATGTTGCCCGCGTGGTGCGCGGCAAGCGTGTGGCGGCCC
ACGTGCGGGCGATGATCGTTCCCGGCTCGACGTTGGTGCGCAATCAGGCCGAGGACGAAGGCCTGGCGCAGATCTTTCTC
GACGCCGGTTTCGAATGGCGGCAATCGGGTTGCTCGATGTGCCTGGCGATGAACGACGACGTGCTCGCGCCAGGTGATCG
CTGTGCGTCGAGCACCAATCGCAACTTCGAAGGCCGTCAGGGCGCCGGTGCGCGCACCCACCTGATGAGCCCGGCGATGG
TCGCCGCCGCCATCAGCGGCCATTTGACTGACGTGCGCACCGTCGCGCTGGAGGCTTGA

Upstream 100 bases:

>100_bases
TCGTGTTTAACCGCTTCACCAATGCGCTCTTGATGGCATTTCTGCGATCAATATTCTGTCCTCATGTTGTTTTTGCCCCG
GAAAGAAAAGAGCGCAAGCC

Downstream 100 bases:

>100_bases
ACCATGCAACCTTTCGACACCGTCAGCGGCAGCGCCGCGCCGTTTCTTGCATCCAACATCGACACCGACGTGATCATGCC
CAAGCAGTTTCTCAAGGGCA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPSPRTLYQKHIDSHTVCTLDDQGHVLLYIDRQVANEYTSPQAFSGLREAGRTVWRPAATLAVVDHVNPTAPKRIATMPD
AGGARQVSYFEENCRDFGIELFDVLDKRQGIEHVVAPEQGFILPGMVVAAGDSHTTTYGALGAFGFGIGTSEIEHLLATQ
TLVYKRLKTLRVTVNGELGAGVTSKDIIMALIEKIGASGATGYAIEFTGPAISALSVEARMTICNMAVEAGARGAFMAPD
DKVFAYLQHKPRAPKGKLWEQAIDHWKTLHSDEGAVFDREVILDVAELEPMVTWGTSPDQAAPIKAHVPDPAAQPDPILR
QGLQRALDYMGLTPGMPLNEVTISHAFIGSCTNARIEDLRDVARVVRGKRVAAHVRAMIVPGSTLVRNQAEDEGLAQIFL
DAGFEWRQSGCSMCLAMNDDVLAPGDRCASSTNRNFEGRQGAGARTHLMSPAMVAAAISGHLTDVRTVALEA

Sequences:

>Translated_472_residues
MPSPRTLYQKHIDSHTVCTLDDQGHVLLYIDRQVANEYTSPQAFSGLREAGRTVWRPAATLAVVDHVNPTAPKRIATMPD
AGGARQVSYFEENCRDFGIELFDVLDKRQGIEHVVAPEQGFILPGMVVAAGDSHTTTYGALGAFGFGIGTSEIEHLLATQ
TLVYKRLKTLRVTVNGELGAGVTSKDIIMALIEKIGASGATGYAIEFTGPAISALSVEARMTICNMAVEAGARGAFMAPD
DKVFAYLQHKPRAPKGKLWEQAIDHWKTLHSDEGAVFDREVILDVAELEPMVTWGTSPDQAAPIKAHVPDPAAQPDPILR
QGLQRALDYMGLTPGMPLNEVTISHAFIGSCTNARIEDLRDVARVVRGKRVAAHVRAMIVPGSTLVRNQAEDEGLAQIFL
DAGFEWRQSGCSMCLAMNDDVLAPGDRCASSTNRNFEGRQGAGARTHLMSPAMVAAAISGHLTDVRTVALEA
>Mature_471_residues
PSPRTLYQKHIDSHTVCTLDDQGHVLLYIDRQVANEYTSPQAFSGLREAGRTVWRPAATLAVVDHVNPTAPKRIATMPDA
GGARQVSYFEENCRDFGIELFDVLDKRQGIEHVVAPEQGFILPGMVVAAGDSHTTTYGALGAFGFGIGTSEIEHLLATQT
LVYKRLKTLRVTVNGELGAGVTSKDIIMALIEKIGASGATGYAIEFTGPAISALSVEARMTICNMAVEAGARGAFMAPDD
KVFAYLQHKPRAPKGKLWEQAIDHWKTLHSDEGAVFDREVILDVAELEPMVTWGTSPDQAAPIKAHVPDPAAQPDPILRQ
GLQRALDYMGLTPGMPLNEVTISHAFIGSCTNARIEDLRDVARVVRGKRVAAHVRAMIVPGSTLVRNQAEDEGLAQIFLD
AGFEWRQSGCSMCLAMNDDVLAPGDRCASSTNRNFEGRQGAGARTHLMSPAMVAAAISGHLTDVRTVALEA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=364, Percent_Identity=30.2197802197802, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI8659555, Length=387, Percent_Identity=26.6149870801034, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI41352693, Length=388, Percent_Identity=26.2886597938144, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=53.0042918454936, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=24.5125348189415, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081781, Length=356, Percent_Identity=23.0337078651685, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=402, Percent_Identity=28.1094527363184, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=362, Percent_Identity=29.2817679558011, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=29.2763157894737, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=389, Percent_Identity=24.4215938303342, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=54.2553191489362, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6322261, Length=396, Percent_Identity=28.2828282828283, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=353, Percent_Identity=29.1784702549575, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=365, Percent_Identity=29.041095890411, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI28571643, Length=389, Percent_Identity=27.2493573264781, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161076999, Length=401, Percent_Identity=26.1845386533666, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281365315, Length=400, Percent_Identity=26.25, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17864292, Length=400, Percent_Identity=26.25, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24645686, Length=454, Percent_Identity=25.3303964757709, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137564, Length=367, Percent_Identity=25.3405994550409, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50875; Mature: 50743

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPRTLYQKHIDSHTVCTLDDQGHVLLYIDRQVANEYTSPQAFSGLREAGRTVWRPAAT
CCCCHHHHHHHCCCCEEEEECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHCHHHHCCHHH
LAVVDHVNPTAPKRIATMPDAGGARQVSYFEENCRDFGIELFDVLDKRQGIEHVVAPEQG
EEEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCEECCCCCC
FILPGMVVAAGDSHTTTYGALGAFGFGIGTSEIEHLLATQTLVYKRLKTLRVTVNGELGA
CCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
GVTSKDIIMALIEKIGASGATGYAIEFTGPAISALSVEARMTICNMAVEAGARGAFMAPD
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCC
DKVFAYLQHKPRAPKGKLWEQAIDHWKTLHSDEGAVFDREVILDVAELEPMVTWGTSPDQ
HHEEHEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCEEEECCCCCC
AAPIKAHVPDPAAQPDPILRQGLQRALDYMGLTPGMPLNEVTISHAFIGSCTNARIEDLR
CCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEEHHHHHCCCCCCHHHHHH
DVARVVRGKRVAAHVRAMIVPGSTLVRNQAEDEGLAQIFLDAGFEWRQSGCSMCLAMNDD
HHHHHHHCCHHHHHHEEEEECCHHHHHCCCCCCHHHHHHHHCCCHHHHCCCCEEEEECCC
VLAPGDRCASSTNRNFEGRQGAGARTHLMSPAMVAAAISGHLTDVRTVALEA
CCCCCHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCHHHHEEEEECC
>Mature Secondary Structure 
PSPRTLYQKHIDSHTVCTLDDQGHVLLYIDRQVANEYTSPQAFSGLREAGRTVWRPAAT
CCCHHHHHHHCCCCEEEEECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHCHHHHCCHHH
LAVVDHVNPTAPKRIATMPDAGGARQVSYFEENCRDFGIELFDVLDKRQGIEHVVAPEQG
EEEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCEECCCCCC
FILPGMVVAAGDSHTTTYGALGAFGFGIGTSEIEHLLATQTLVYKRLKTLRVTVNGELGA
CCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
GVTSKDIIMALIEKIGASGATGYAIEFTGPAISALSVEARMTICNMAVEAGARGAFMAPD
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCC
DKVFAYLQHKPRAPKGKLWEQAIDHWKTLHSDEGAVFDREVILDVAELEPMVTWGTSPDQ
HHEEHEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCEEEECCCCCC
AAPIKAHVPDPAAQPDPILRQGLQRALDYMGLTPGMPLNEVTISHAFIGSCTNARIEDLR
CCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEEHHHHHCCCCCCHHHHHH
DVARVVRGKRVAAHVRAMIVPGSTLVRNQAEDEGLAQIFLDAGFEWRQSGCSMCLAMNDD
HHHHHHHCCHHHHHHEEEEECCHHHHHCCCCCCHHHHHHHHCCCHHHHCCCCEEEEECCC
VLAPGDRCASSTNRNFEGRQGAGARTHLMSPAMVAAAISGHLTDVRTVALEA
CCCCCHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA