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The map label for this gene is leuD1 [H]

Identifier: 77458586

GI number: 77458586

Start: 2717244

End: 2717852

Strand: Reverse

Name: leuD1 [H]

Synonym: Pfl01_2359

Alternate gene names: 77458586

Gene position: 2717852-2717244 (Counterclockwise)

Preceding gene: 77458587

Following gene: 77458584

Centisome position: 42.21

GC content: 61.9

Gene sequence:

>609_bases
ATGCAACCTTTCGACACCGTCAGCGGCAGCGCCGCGCCGTTTCTTGCATCCAACATCGACACCGACGTGATCATGCCCAA
GCAGTTTCTCAAGGGCATCGATCGTCAGGGGCTGGATCGCGGGCTGTTCTTCGATCTGCGCTTTCTCGCCTCCGGCGAAC
CCAACCCTGAATTCGTGCTCAACCAGCCGGCCTGGCAGGACGCGGCGTTTCTGGTGACCGGCCCCAACTTCGGTTGCGGC
TCCAGCCGCGAACACGCGGTATGGGGCTTGAAGCAAGTGGGAATCCGGGCCTTGATCGGCACCACCTTCGCCGGGATTTT
TTACGACAACTGCCAGCGCAATGGTGTGTTGGCGATTCAGCTGGACGACGCGCAGTTCAAGCGTGTAGCCGAGGCGATCA
GCGTTCCGGCGACCGCGCGGATCAGCGTTAACCTGGCGCAGCAGACCCTCGAGCTGGCGGACGGTACGCTGATCGAATTC
GAGATCGATCAGTTGCGCAAACAGTCGTTGCTGCTCGGGCTGGACGCCATCGGCACCACGCTGCAACGCACCGAGCAGAT
CCGCGCCTTTGAGGCACGGCATCTGGCGGACAATCCCTGGTTGGGTTGA

Upstream 100 bases:

>100_bases
CGCCGGTGCGCGCACCCACCTGATGAGCCCGGCGATGGTCGCCGCCGCCATCAGCGGCCATTTGACTGACGTGCGCACCG
TCGCGCTGGAGGCTTGAACC

Downstream 100 bases:

>100_bases
TGGCAAAAAAACGGGCGGTTCATTGAACCGCCCGTTTTCATTCCACGGACAAATCGCGGAAGTACTCTTCCAGAAACTCC
ACGCATACCCGCAGCTTTGC

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MQPFDTVSGSAAPFLASNIDTDVIMPKQFLKGIDRQGLDRGLFFDLRFLASGEPNPEFVLNQPAWQDAAFLVTGPNFGCG
SSREHAVWGLKQVGIRALIGTTFAGIFYDNCQRNGVLAIQLDDAQFKRVAEAISVPATARISVNLAQQTLELADGTLIEF
EIDQLRKQSLLLGLDAIGTTLQRTEQIRAFEARHLADNPWLG

Sequences:

>Translated_202_residues
MQPFDTVSGSAAPFLASNIDTDVIMPKQFLKGIDRQGLDRGLFFDLRFLASGEPNPEFVLNQPAWQDAAFLVTGPNFGCG
SSREHAVWGLKQVGIRALIGTTFAGIFYDNCQRNGVLAIQLDDAQFKRVAEAISVPATARISVNLAQQTLELADGTLIEF
EIDQLRKQSLLLGLDAIGTTLQRTEQIRAFEARHLADNPWLG
>Mature_202_residues
MQPFDTVSGSAAPFLASNIDTDVIMPKQFLKGIDRQGLDRGLFFDLRFLASGEPNPEFVLNQPAWQDAAFLVTGPNFGCG
SSREHAVWGLKQVGIRALIGTTFAGIFYDNCQRNGVLAIQLDDAQFKRVAEAISVPATARISVNLAQQTLELADGTLIEF
EIDQLRKQSLLLGLDAIGTTLQRTEQIRAFEARHLADNPWLG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=40.6091370558376, Blast_Score=151, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=44.7236180904523, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22148; Mature: 22148

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFDTVSGSAAPFLASNIDTDVIMPKQFLKGIDRQGLDRGLFFDLRFLASGEPNPEFVL
CCCCCCCCCCCCCHHHCCCCCCEECHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEE
NQPAWQDAAFLVTGPNFGCGSSREHAVWGLKQVGIRALIGTTFAGIFYDNCQRNGVLAIQ
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LDDAQFKRVAEAISVPATARISVNLAQQTLELADGTLIEFEIDQLRKQSLLLGLDAIGTT
ECHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHH
LQRTEQIRAFEARHLADNPWLG
HHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQPFDTVSGSAAPFLASNIDTDVIMPKQFLKGIDRQGLDRGLFFDLRFLASGEPNPEFVL
CCCCCCCCCCCCCHHHCCCCCCEECHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEE
NQPAWQDAAFLVTGPNFGCGSSREHAVWGLKQVGIRALIGTTFAGIFYDNCQRNGVLAIQ
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LDDAQFKRVAEAISVPATARISVNLAQQTLELADGTLIEFEIDQLRKQSLLLGLDAIGTT
ECHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHH
LQRTEQIRAFEARHLADNPWLG
HHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA