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The map label for this gene is xylX [H]

Identifier: 77458548

GI number: 77458548

Start: 2677247

End: 2678605

Strand: Direct

Name: xylX [H]

Synonym: Pfl01_2321

Alternate gene names: 77458548

Gene position: 2677247-2678605 (Clockwise)

Preceding gene: 77458547

Following gene: 77458549

Centisome position: 41.58

GC content: 60.85

Gene sequence:

>1359_bases
ATGTCCCTGGGAATCGACTACCTCAATGCCATGCTTGAAGAGGACCCCGAGAAGGGGGCCTACCGCTGCAAGCGGGAGAT
GTTCACCGATCCGCGGCTGTTCGAACTGGAGATGGCGCACATCTTCGAAGGCAACTGGATCTACCTCGCCCATGAAAGCC
AGATCCCCAACGTCAATGACTTTCTGACCACCACCATGGGCCGCCAACCCGTGTTCATTGCCCGCAACAAGGCCGGTGAA
CTCAATGCCTTTCTCAATGCGTGCAGCCATCGCGGCGCCATGCTTTGCCGGCACAAGTCCGGCAACCGCTCCAGCTATAC
CTGCCCGTTCCACGGCTGGACGTTCAACAACAGCGGCAAACTGCTCAAGGTCAAGGACCCGAGCGAGGCCGGCTACCCCG
AAGGCTTCAACTGCGAAGGCTCCCATGACCTGACCAAAGTCGCGCGTTTCGAGTCCTATCGCGGCTTTCTGTTCGGCAGC
CTGAACGCCGATGTGAAATCCCTCGCCGAGCACCTGGGCGAGTCGGCCAAGATCATCGACATGATCGTCGATCAGTCCCC
TGAAGGCTTGGAAGTGCTGCGCGGTTCCAGCTCCTACATCTATGAAGGCAACTGGAAACTCACCGCCGAAAATGGCGCCG
ATGGCTATCACGTCAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGTCAGCAGCGCGACGCTGGCGAAGAA
ATCAAGACCATGAGCGCCGGCAGTTGGGCGAAGAAGGGCGGCGGTTTCTACTCGTTCGACCACGGCCACTTGCTGCTCTG
GACCCGCTGGGCCAACCCCGAGGACCGTCCGGCCTACGAGCGTCGCGATGAACTGGTCCGGGACTTTGGCCAGGCTCGCG
CTGACTGGATGATCGAGAACTCGCGCAACCTGTGCCTGTACCCCAACGTGTATCTGATGGATCAGTTCAGCTCGCAGATC
CGCATCGCCCGGCCGATCTCCGTCGACAAGACCGAGATCACCATCTATTGCATTGCCCCCAAAGGCGAGAGCGCCGAGGC
CCGGGCCAAGCGCATTCGCCAATACGAAGACTTCTTCAACGTCAGCGGTATGGCCACCCCGGATGACCTCGAAGAGTTCC
GCTCATGCCAGACCGGTTACGGCGCCGGGCGCGGCTGGAACGACATGTCCCGTGGCGCGACCCATTGGGTCGAAGGCGCA
GACGCGGCGGCCGAGGAAATCGACCTCAAGCCCTTGCTGTCCGGCATGCGCACCGAGGACGAAGGCCTGTTCGTGCTGCA
ACACAAGTACTGGCAGGAAACCATGCTCAAGGCCACCGCCAGCGATAAAAACCTGATCCCCGTGGAGGCCGTGCAATGA

Upstream 100 bases:

>100_bases
CTGCACAAAACGGATAGCGCTCTGCAGGATTCGGATATTGCCGCCCGCGTTGCCTCCCTAATCTGACCTGGCCTGAACAA
TAACAATGGAGGCCCCGGCC

Downstream 100 bases:

>100_bases
GCAACCTCTACGACACCGTGCGCGATTTTCTCTACCGCGAAGCACGTTATCTGGACGACGGACAGTGGGATCAGTGGCTG
GAGCTCTACGCTGCCGACGC

Product: benzoate 1,2-dioxygenase subunit alpha

Products: 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate; NAD+

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSLGIDYLNAMLEEDPEKGAYRCKREMFTDPRLFELEMAHIFEGNWIYLAHESQIPNVNDFLTTTMGRQPVFIARNKAGE
LNAFLNACSHRGAMLCRHKSGNRSSYTCPFHGWTFNNSGKLLKVKDPSEAGYPEGFNCEGSHDLTKVARFESYRGFLFGS
LNADVKSLAEHLGESAKIIDMIVDQSPEGLEVLRGSSSYIYEGNWKLTAENGADGYHVSSVHWNYAATQNQRQQRDAGEE
IKTMSAGSWAKKGGGFYSFDHGHLLLWTRWANPEDRPAYERRDELVRDFGQARADWMIENSRNLCLYPNVYLMDQFSSQI
RIARPISVDKTEITIYCIAPKGESAEARAKRIRQYEDFFNVSGMATPDDLEEFRSCQTGYGAGRGWNDMSRGATHWVEGA
DAAAEEIDLKPLLSGMRTEDEGLFVLQHKYWQETMLKATASDKNLIPVEAVQ

Sequences:

>Translated_452_residues
MSLGIDYLNAMLEEDPEKGAYRCKREMFTDPRLFELEMAHIFEGNWIYLAHESQIPNVNDFLTTTMGRQPVFIARNKAGE
LNAFLNACSHRGAMLCRHKSGNRSSYTCPFHGWTFNNSGKLLKVKDPSEAGYPEGFNCEGSHDLTKVARFESYRGFLFGS
LNADVKSLAEHLGESAKIIDMIVDQSPEGLEVLRGSSSYIYEGNWKLTAENGADGYHVSSVHWNYAATQNQRQQRDAGEE
IKTMSAGSWAKKGGGFYSFDHGHLLLWTRWANPEDRPAYERRDELVRDFGQARADWMIENSRNLCLYPNVYLMDQFSSQI
RIARPISVDKTEITIYCIAPKGESAEARAKRIRQYEDFFNVSGMATPDDLEEFRSCQTGYGAGRGWNDMSRGATHWVEGA
DAAAEEIDLKPLLSGMRTEDEGLFVLQHKYWQETMLKATASDKNLIPVEAVQ
>Mature_451_residues
SLGIDYLNAMLEEDPEKGAYRCKREMFTDPRLFELEMAHIFEGNWIYLAHESQIPNVNDFLTTTMGRQPVFIARNKAGEL
NAFLNACSHRGAMLCRHKSGNRSSYTCPFHGWTFNNSGKLLKVKDPSEAGYPEGFNCEGSHDLTKVARFESYRGFLFGSL
NADVKSLAEHLGESAKIIDMIVDQSPEGLEVLRGSSSYIYEGNWKLTAENGADGYHVSSVHWNYAATQNQRQQRDAGEEI
KTMSAGSWAKKGGGFYSFDHGHLLLWTRWANPEDRPAYERRDELVRDFGQARADWMIENSRNLCLYPNVYLMDQFSSQIR
IARPISVDKTEITIYCIAPKGESAEARAKRIRQYEDFFNVSGMATPDDLEEFRSCQTGYGAGRGWNDMSRGATHWVEGAD
AAAEEIDLKPLLSGMRTEDEGLFVLQHKYWQETMLKATASDKNLIPVEAVQ

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=371, Percent_Identity=29.9191374663073, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1788103, Length=205, Percent_Identity=27.3170731707317, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.12.10

Molecular weight: Translated: 51183; Mature: 51052

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGIDYLNAMLEEDPEKGAYRCKREMFTDPRLFELEMAHIFEGNWIYLAHESQIPNVND
CCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEECCCCCCHHH
FLTTTMGRQPVFIARNKAGELNAFLNACSHRGAMLCRHKSGNRSSYTCPFHGWTFNNSGK
HHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCEEECCCCC
LLKVKDPSEAGYPEGFNCEGSHDLTKVARFESYRGFLFGSLNADVKSLAEHLGESAKIID
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCEEEH
MIVDQSPEGLEVLRGSSSYIYEGNWKLTAENGADGYHVSSVHWNYAATQNQRQQRDAGEE
HHHCCCCCCHHHHCCCCCEEEECCEEEEECCCCCCEEEEEEEEEEHHCCHHHHHHHHHHH
IKTMSAGSWAKKGGGFYSFDHGHLLLWTRWANPEDRPAYERRDELVRDFGQARADWMIEN
HHHCCCCCCHHCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCEEEEC
SRNLCLYPNVYLMDQFSSQIRIARPISVDKTEITIYCIAPKGESAEARAKRIRQYEDFFN
CCCEEECCCEEEEECCCCCEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHC
VSGMATPDDLEEFRSCQTGYGAGRGWNDMSRGATHWVEGADAAAEEIDLKPLLSGMRTED
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHCCCCHHHHHCCHHHHHHCCCCCC
EGLFVLQHKYWQETMLKATASDKNLIPVEAVQ
CCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SLGIDYLNAMLEEDPEKGAYRCKREMFTDPRLFELEMAHIFEGNWIYLAHESQIPNVND
CCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEECCCCCCHHH
FLTTTMGRQPVFIARNKAGELNAFLNACSHRGAMLCRHKSGNRSSYTCPFHGWTFNNSGK
HHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCEEECCCCC
LLKVKDPSEAGYPEGFNCEGSHDLTKVARFESYRGFLFGSLNADVKSLAEHLGESAKIID
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCEEEH
MIVDQSPEGLEVLRGSSSYIYEGNWKLTAENGADGYHVSSVHWNYAATQNQRQQRDAGEE
HHHCCCCCCHHHHCCCCCEEEECCEEEEECCCCCCEEEEEEEEEEHHCCHHHHHHHHHHH
IKTMSAGSWAKKGGGFYSFDHGHLLLWTRWANPEDRPAYERRDELVRDFGQARADWMIEN
HHHCCCCCCHHCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCEEEEC
SRNLCLYPNVYLMDQFSSQIRIARPISVDKTEITIYCIAPKGESAEARAKRIRQYEDFFN
CCCEEECCCEEEEECCCCCEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHC
VSGMATPDDLEEFRSCQTGYGAGRGWNDMSRGATHWVEGADAAAEEIDLKPLLSGMRTED
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHCCCCHHHHHCCHHHHHHCCCCCC
EGLFVLQHKYWQETMLKATASDKNLIPVEAVQ
CCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: benzoate; NADH; H+; O2

Specific reaction: benzoate + NADH + H+ + O2 = 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]