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The map label for this gene is dadA [H]

Identifier: 77458532

GI number: 77458532

Start: 2658134

End: 2659396

Strand: Reverse

Name: dadA [H]

Synonym: Pfl01_2305

Alternate gene names: 77458532

Gene position: 2659396-2658134 (Counterclockwise)

Preceding gene: 77458533

Following gene: 77458530

Centisome position: 41.31

GC content: 63.42

Gene sequence:

>1263_bases
ATGCCGAATAACCCGTACGACATCGCCGTGGTCGGCGCCGGCATCATCGGTGTCGCGAGTGCCCTGCGCCTGGCCCGTCA
GGGTTTGCGGGTGGTGGTGATCGACCCGCAGGAACCGGGCCACGGCGCCTCGTTCGGCAACGCCGGACACCTGGCGACCG
AGCAGGTGTTTCCGATTGCCGACAGCTCGATCCTCAAACGCCTGCCGGCCATGCTCATGGACCCGATGGGCCCGCTGCGT
CTGGACTGGAAATACCTGCCCCGCGCCCTGCCCTGGTTCGCACGGCTGTTGCTGAACCTGCGCTCGGCACCGTTCCAGCG
CACCGTTGCCGGGTTGCGCGCGCTGAATGAAAGCTCGCTGGATGCCTGGCACCGCTTGTTGAATGACATTCAGCGTCCCG
AGCTGTTGAAGATGGAAGGTTCGCTGCTGGTGTTCGAACGACCCGACTCACGCCCGGCCCTTGAAGCAGTACAGCGACGC
ATGCAACAGCAACAAGTGCCAGTCGATGGGTGGCAGGCCGGAGCGGTACGCGAGACCGCGCCGCAACTCAGCGAACAGAT
TCAGGGCGGACTGTTTTTCCCGAACACCGGGCATTTCATCGATCCCTACCGCGTCGTTTGTGAGTTGGTCGAGGCGGCGA
AAGCCAGCGGCGTGCAGTTTGTGAAACAGGCCGTTCAGGGCGGGCAACTGCACGAGCATGGCGTCAATCTGATGACCGGA
ACCGGCGCCCTGTCCGCTCGCCAGGTACTGATCGCCTGTGGCGCCCACTCGGCAAAACTCACCGCTGCACTGACTGGCAA
AAAAGTCCCGCTGGACACCGAGCGCGGCTATCACCTGATGCTGCCCCACGAACACAACCGGCTGCCTTTCCCCGTCACCT
CGCTGGAACGCAAATTCATCATGACCCCGATGTCCGACGGGCTGCGCCTGGCCGGCACCGTGGAATTCGCCGGCCTCGAC
GCCCCTCCGACCATGGCCCGCGCCTGGCAGTTACATCGATTGAGCCAAGGGTTGTTTCGTGAGGATTTGAGTGTCGAGGC
AGCCACACCGTGGATGGGTTTTCGACCGTCGCTGCCGGATTCATTGCCGGTGATTGATCGGGTGTGTGACGGCAAAGTAT
TGCTTGCGTTCGGGCATCAGCATCTGGGGCTGACGCAGGCGGCGGTGACGGCGGAGATGGTTGGGGGGATGGTATTGCCG
TCGGCGCAGGACACGGCGCGTATTCTCCCGTCGAGCGAGCCCTATCGGCTGGATCGCTTTTGA

Upstream 100 bases:

>100_bases
TATCAGGATTTCCCCGACAGCCTGCTGCCGTCCGCGCTCAAACACGCCAATCCACTGTCGCTGCGTCGCTTGCTCGATGG
CCAAAGGGAAACCGCGAAAA

Downstream 100 bases:

>100_bases
GTGGCCGGCTATCGGGCGCCCTGCTGATATTCACGGGGCGTACACCCGAACTCCCGGCGAAACACCGTGTGCAGGTACTG
TGCGGATTTGAAACCGCACG

Product: D-amino-acid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MPNNPYDIAVVGAGIIGVASALRLARQGLRVVVIDPQEPGHGASFGNAGHLATEQVFPIADSSILKRLPAMLMDPMGPLR
LDWKYLPRALPWFARLLLNLRSAPFQRTVAGLRALNESSLDAWHRLLNDIQRPELLKMEGSLLVFERPDSRPALEAVQRR
MQQQQVPVDGWQAGAVRETAPQLSEQIQGGLFFPNTGHFIDPYRVVCELVEAAKASGVQFVKQAVQGGQLHEHGVNLMTG
TGALSARQVLIACGAHSAKLTAALTGKKVPLDTERGYHLMLPHEHNRLPFPVTSLERKFIMTPMSDGLRLAGTVEFAGLD
APPTMARAWQLHRLSQGLFREDLSVEAATPWMGFRPSLPDSLPVIDRVCDGKVLLAFGHQHLGLTQAAVTAEMVGGMVLP
SAQDTARILPSSEPYRLDRF

Sequences:

>Translated_420_residues
MPNNPYDIAVVGAGIIGVASALRLARQGLRVVVIDPQEPGHGASFGNAGHLATEQVFPIADSSILKRLPAMLMDPMGPLR
LDWKYLPRALPWFARLLLNLRSAPFQRTVAGLRALNESSLDAWHRLLNDIQRPELLKMEGSLLVFERPDSRPALEAVQRR
MQQQQVPVDGWQAGAVRETAPQLSEQIQGGLFFPNTGHFIDPYRVVCELVEAAKASGVQFVKQAVQGGQLHEHGVNLMTG
TGALSARQVLIACGAHSAKLTAALTGKKVPLDTERGYHLMLPHEHNRLPFPVTSLERKFIMTPMSDGLRLAGTVEFAGLD
APPTMARAWQLHRLSQGLFREDLSVEAATPWMGFRPSLPDSLPVIDRVCDGKVLLAFGHQHLGLTQAAVTAEMVGGMVLP
SAQDTARILPSSEPYRLDRF
>Mature_419_residues
PNNPYDIAVVGAGIIGVASALRLARQGLRVVVIDPQEPGHGASFGNAGHLATEQVFPIADSSILKRLPAMLMDPMGPLRL
DWKYLPRALPWFARLLLNLRSAPFQRTVAGLRALNESSLDAWHRLLNDIQRPELLKMEGSLLVFERPDSRPALEAVQRRM
QQQQVPVDGWQAGAVRETAPQLSEQIQGGLFFPNTGHFIDPYRVVCELVEAAKASGVQFVKQAVQGGQLHEHGVNLMTGT
GALSARQVLIACGAHSAKLTAALTGKKVPLDTERGYHLMLPHEHNRLPFPVTSLERKFIMTPMSDGLRLAGTVEFAGLDA
PPTMARAWQLHRLSQGLFREDLSVEAATPWMGFRPSLPDSLPVIDRVCDGKVLLAFGHQHLGLTQAAVTAEMVGGMVLPS
AQDTARILPSSEPYRLDRF

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=407, Percent_Identity=25.5528255528256, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45936; Mature: 45805

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNPYDIAVVGAGIIGVASALRLARQGLRVVVIDPQEPGHGASFGNAGHLATEQVFPIA
CCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
DSSILKRLPAMLMDPMGPLRLDWKYLPRALPWFARLLLNLRSAPFQRTVAGLRALNESSL
CHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHH
DAWHRLLNDIQRPELLKMEGSLLVFERPDSRPALEAVQRRMQQQQVPVDGWQAGAVRETA
HHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
PQLSEQIQGGLFFPNTGHFIDPYRVVCELVEAAKASGVQFVKQAVQGGQLHEHGVNLMTG
HHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHCCCEEEEC
TGALSARQVLIACGAHSAKLTAALTGKKVPLDTERGYHLMLPHEHNRLPFPVTSLERKFI
CCCHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCEEE
MTPMSDGLRLAGTVEFAGLDAPPTMARAWQLHRLSQGLFREDLSVEAATPWMGFRPSLPD
ECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCC
SLPVIDRVCDGKVLLAFGHQHLGLTQAAVTAEMVGGMVLPSAQDTARILPSSEPYRLDRF
CCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCEECCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PNNPYDIAVVGAGIIGVASALRLARQGLRVVVIDPQEPGHGASFGNAGHLATEQVFPIA
CCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
DSSILKRLPAMLMDPMGPLRLDWKYLPRALPWFARLLLNLRSAPFQRTVAGLRALNESSL
CHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHH
DAWHRLLNDIQRPELLKMEGSLLVFERPDSRPALEAVQRRMQQQQVPVDGWQAGAVRETA
HHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
PQLSEQIQGGLFFPNTGHFIDPYRVVCELVEAAKASGVQFVKQAVQGGQLHEHGVNLMTG
HHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHCCCEEEEC
TGALSARQVLIACGAHSAKLTAALTGKKVPLDTERGYHLMLPHEHNRLPFPVTSLERKFI
CCCHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCEEE
MTPMSDGLRLAGTVEFAGLDAPPTMARAWQLHRLSQGLFREDLSVEAATPWMGFRPSLPD
ECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCC
SLPVIDRVCDGKVLLAFGHQHLGLTQAAVTAEMVGGMVLPSAQDTARILPSSEPYRLDRF
CCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCEECCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA