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The map label for this gene is ilvA [H]

Identifier: 77458452

GI number: 77458452

Start: 2547930

End: 2548886

Strand: Direct

Name: ilvA [H]

Synonym: Pfl01_2225

Alternate gene names: 77458452

Gene position: 2547930-2548886 (Clockwise)

Preceding gene: 77458449

Following gene: 77458453

Centisome position: 39.57

GC content: 59.67

Gene sequence:

>957_bases
ATGCACGTATTCACTCGCGACGACATCGAAAACGCCGCCCGCCAGGTTTATCAGGTGATGTCGGCCACCGCCCAATACCG
TTGGCCATTGCTCGCCGAGCGGTTGGGCTGCACCGTTTGGGTCAAGCATGAAAACCACACGCCGACCGGCGCCTTCAAAG
TGCGTGGCGGTATTACCTTTATGCACTGGCTGCGTCGTGAGCATCCGACTGTCAAAGGCATCGTTTCTGCGACACGTGGC
AATCACGGTCAGAGTCTGGCGCTGGCGGCGCGGGCCGTGGGACTGAAAGCGTTGATCGTGGTGCCGCAGGGCAACTCGCT
GGAGAAGAACAACGCCATGCGTGGCTTTGGTGGTGAAGTGGTCGAATACGGGCGCGATTTTGACGAGGCTCGTGAAGAGG
CGGCGCGCCTGGCACAGAAGCACGACCTTTTTCTGGTGCCACCGTTTCACGTTGAACTGGTCAAGGGCGTAGCGACCTAT
GCGCTGGAGCTGTTCGGTGCGGCGCCGGATCTGGATACGGTTTACGTGCCCATTGGTTGCGGATCGGGGATCTGTGGTGT
GATCGCTGCCCGCGACGCCCTAGGACTGAATACCGAAGTGGTGGGGGTGGTGTCGACAGAGGCCGCGGCCGCCAAGCTGT
CGTTTGAAGCGAACGCGATTTGCGAAACCGCTTCGGCGAATACGTTCGCGGATGGTTTGGCTGTTCGCAAGCCGATTGCG
CAGGCGTTCGATATCTATAAAGAAGGGGCGGCGAGGATTCTCTCGGTCAGCGAAGTTGAGATTGCCGAAGCCATGCGCGT
GTATTACACCGACACCCACAATCTTGCGGAGGGCGCGGGTGCCGCAGCGTTGGCGGCGTTGATGCAGGATCGGGGGAGGA
TGGAGGGGAAAAAGGTCGGGGTGGTTCTGTCCGGAGGGAATATTGACCGATCGGTGTATGCAAAGGTGATTGGTTGA

Upstream 100 bases:

>100_bases
AATAGTAGTGAAGGGGGCGGGTAAAAGTCAGGTCGGTTTTTTGAAAGCCGACGTGATGCGTCCGTGCCTAGACTGGGCCA
ACGTCCAAGGAGCCACTTAT

Downstream 100 bases:

>100_bases
TTGCTGATGTGCTGGTTGGCGGGCTTGGCATTCACTGACAGCAATTCGTTCGCGTCTAACATAGAAGCGGCCTTTTTCAT
TTCTTGATAGAAAAAGCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MHVFTRDDIENAARQVYQVMSATAQYRWPLLAERLGCTVWVKHENHTPTGAFKVRGGITFMHWLRREHPTVKGIVSATRG
NHGQSLALAARAVGLKALIVVPQGNSLEKNNAMRGFGGEVVEYGRDFDEAREEAARLAQKHDLFLVPPFHVELVKGVATY
ALELFGAAPDLDTVYVPIGCGSGICGVIAARDALGLNTEVVGVVSTEAAAAKLSFEANAICETASANTFADGLAVRKPIA
QAFDIYKEGAARILSVSEVEIAEAMRVYYTDTHNLAEGAGAAALAALMQDRGRMEGKKVGVVLSGGNIDRSVYAKVIG

Sequences:

>Translated_318_residues
MHVFTRDDIENAARQVYQVMSATAQYRWPLLAERLGCTVWVKHENHTPTGAFKVRGGITFMHWLRREHPTVKGIVSATRG
NHGQSLALAARAVGLKALIVVPQGNSLEKNNAMRGFGGEVVEYGRDFDEAREEAARLAQKHDLFLVPPFHVELVKGVATY
ALELFGAAPDLDTVYVPIGCGSGICGVIAARDALGLNTEVVGVVSTEAAAAKLSFEANAICETASANTFADGLAVRKPIA
QAFDIYKEGAARILSVSEVEIAEAMRVYYTDTHNLAEGAGAAALAALMQDRGRMEGKKVGVVLSGGNIDRSVYAKVIG
>Mature_318_residues
MHVFTRDDIENAARQVYQVMSATAQYRWPLLAERLGCTVWVKHENHTPTGAFKVRGGITFMHWLRREHPTVKGIVSATRG
NHGQSLALAARAVGLKALIVVPQGNSLEKNNAMRGFGGEVVEYGRDFDEAREEAARLAQKHDLFLVPPFHVELVKGVATY
ALELFGAAPDLDTVYVPIGCGSGICGVIAARDALGLNTEVVGVVSTEAAAAKLSFEANAICETASANTFADGLAVRKPIA
QAFDIYKEGAARILSVSEVEIAEAMRVYYTDTHNLAEGAGAAALAALMQDRGRMEGKKVGVVLSGGNIDRSVYAKVIG

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=311, Percent_Identity=28.2958199356913, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1790207, Length=297, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789505, Length=315, Percent_Identity=29.8412698412698, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71991565, Length=312, Percent_Identity=27.8846153846154, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17537387, Length=312, Percent_Identity=30.4487179487179, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508781, Length=280, Percent_Identity=28.2142857142857, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322631, Length=319, Percent_Identity=31.0344827586207, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6320930, Length=278, Percent_Identity=30.2158273381295, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355833, Length=284, Percent_Identity=30.9859154929577, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24645328, Length=219, Percent_Identity=31.5068493150685, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 34038; Mature: 34038

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVFTRDDIENAARQVYQVMSATAQYRWPLLAERLGCTVWVKHENHTPTGAFKVRGGITF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCCCCEEEEECCHHH
MHWLRREHPTVKGIVSATRGNHGQSLALAARAVGLKALIVVPQGNSLEKNNAMRGFGGEV
HHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCHH
VEYGRDFDEAREEAARLAQKHDLFLVPPFHVELVKGVATYALELFGAAPDLDTVYVPIGC
HHHCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GSGICGVIAARDALGLNTEVVGVVSTEAAAAKLSFEANAICETASANTFADGLAVRKPIA
CCCHHHHHHHHHHCCCCCEEEEEEECCHHHHEEECCCHHHCCCCCCCHHHHHHHHHHHHH
QAFDIYKEGAARILSVSEVEIAEAMRVYYTDTHNLAEGAGAAALAALMQDRGRMEGKKVG
HHHHHHHHHHHHEEEHHHHHHHHHHEEEECCCCCHHHCCHHHHHHHHHHHHCCCCCCEEE
VVLSGGNIDRSVYAKVIG
EEEECCCCCHHHHHEECC
>Mature Secondary Structure
MHVFTRDDIENAARQVYQVMSATAQYRWPLLAERLGCTVWVKHENHTPTGAFKVRGGITF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCCCCEEEEECCHHH
MHWLRREHPTVKGIVSATRGNHGQSLALAARAVGLKALIVVPQGNSLEKNNAMRGFGGEV
HHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCHH
VEYGRDFDEAREEAARLAQKHDLFLVPPFHVELVKGVATYALELFGAAPDLDTVYVPIGC
HHHCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GSGICGVIAARDALGLNTEVVGVVSTEAAAAKLSFEANAICETASANTFADGLAVRKPIA
CCCHHHHHHHHHHCCCCCEEEEEEECCHHHHEEECCCHHHCCCCCCCHHHHHHHHHHHHH
QAFDIYKEGAARILSVSEVEIAEAMRVYYTDTHNLAEGAGAAALAALMQDRGRMEGKKVG
HHHHHHHHHHHHEEEHHHHHHHHHHEEEECCCCCHHHCCHHHHHHHHHHHHCCCCCCEEE
VVLSGGNIDRSVYAKVIG
EEEECCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]