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The map label for this gene is arsB [H]

Identifier: 77458420

GI number: 77458420

Start: 2476463

End: 2477746

Strand: Direct

Name: arsB [H]

Synonym: Pfl01_2193

Alternate gene names: 77458420

Gene position: 2476463-2477746 (Clockwise)

Preceding gene: 77458419

Following gene: 77458422

Centisome position: 38.46

GC content: 61.68

Gene sequence:

>1284_bases
ATGCTCGCTGCGTCACTGATTTTCCTGCTGACCATTACCCTGGTGATCTGGCAACCCAAAGGCCTCGGGGTCGGCTGGAG
TGCAACGTTCGGCGCCATCGTTGCGCTGATTTTCGGCGTGGTGCACCTGAGCGACATCCCGCTGGTGTGGCAAATCATCT
GGAACGCTACCGGTACGTTTGTGGCGCTGATCATCATCAGTCTGTTGCTCGACGAGGCCGGTTTTTTCGCCTGGGCCGCC
CTGCACGTAGCGCGTTGGGGGCGGGGCAGCGGGCGCAAGCTGTTTGCCTTCATGGTGCTGCTCGGTGCACTGGTGTCGGC
GCTGTTCGCCAATGACGGCGCGGCGCTGATCCTCACGCCTATCGTGATTTCCATGCTGCTGGCCCTGCGTTTTTCCCCGG
CGGCGACCCTGGCGTTCGTCATGGGCGCGGGCTTCATTGCCGACACCGCGAGCCTGCCGCTGGTGGTGTCGAACCTGGTG
AACATCGTCTCGGCCGACTTCTTTCACATCGGTTTCAATCGCTATGCGGCGGTGATGGTGCCAGTCAACTTCGTCAGCGT
CGCGGCCACGCTGGGCATGTTGCTGTGGTTCTTCCGCCGCGACATTCCCAAGGCCTACGACCCGGAGCAACTTGAACACC
CGGAAACCGCCATCCACGACAAAGCCACGTTCTACGCCGGTTGGGCGGTGCTGGTCATTCTGCTGATCGGCTGTTTCGCG
CTGGAGCCGCTGGGAATCCCGATCAGTGCAATCTCGGCGGTGTGCGCGGCGCTGTTGCTGGCGATTGCCGCGCGCGGGCA
CAAGATCTCCACGCGCAAGGTGATGAAAGAGGCGCCGTGGCAGATCGTGATTTTCTCGCTGGGCATGTATTTGGTGGTCT
ACGGGCTACGCAATGCCGGGCTCACTGATTATCTGGCCGGGTGGCTCAATGTGTTCGCCGGTCACGGTGTGTGGGGCGCG
GCGATGGGCACCGGGGTGCTGACGGCCTTGTTGTCGTCGATCATGAACAACCTGCCGACGGTGCTGGTCGGCCTGCTGTC
GATCGATGCCAGCCAGGCTACGGGTGTGGTGAAGGAAGCGATGATCTACGCCAACGTGATCGGCAGCGACCTGGGCCCGA
AAATCACCCCGATCGGCAGTCTGGCGACTCTGCTCTGGCTGCATGTGCTGGAGCGCAAAGATATCCGGATTGGCTGGGGT
TATTACTTCAAGGTCGGGATTGTGCTGACGGTGCCGGTGTTGCTGGTGACATTGGCTGCTCTGGCGTTGCGGTTGAGCGT
CTAA

Upstream 100 bases:

>100_bases
TGGCAATATTTCTGACACGATAACGAACCTGTGGGGGCTGGCTTCCACAGGTTTTTTTTGTGTGGTCTGATTCTTTCCCT
TTTATTGAAGGCTGTCGCGA

Downstream 100 bases:

>100_bases
CTCTGCCCGGGCACGTTCGGCCACAGGTCCGACACCAGAAACAGCCGCTCGGCTTCTTCCCACTCGCCCCGGGCATTTTC
GGTCAGGCGCACCATCAATT

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLAASLIFLLTITLVIWQPKGLGVGWSATFGAIVALIFGVVHLSDIPLVWQIIWNATGTFVALIIISLLLDEAGFFAWAA
LHVARWGRGSGRKLFAFMVLLGALVSALFANDGAALILTPIVISMLLALRFSPAATLAFVMGAGFIADTASLPLVVSNLV
NIVSADFFHIGFNRYAAVMVPVNFVSVAATLGMLLWFFRRDIPKAYDPEQLEHPETAIHDKATFYAGWAVLVILLIGCFA
LEPLGIPISAISAVCAALLLAIAARGHKISTRKVMKEAPWQIVIFSLGMYLVVYGLRNAGLTDYLAGWLNVFAGHGVWGA
AMGTGVLTALLSSIMNNLPTVLVGLLSIDASQATGVVKEAMIYANVIGSDLGPKITPIGSLATLLWLHVLERKDIRIGWG
YYFKVGIVLTVPVLLVTLAALALRLSV

Sequences:

>Translated_427_residues
MLAASLIFLLTITLVIWQPKGLGVGWSATFGAIVALIFGVVHLSDIPLVWQIIWNATGTFVALIIISLLLDEAGFFAWAA
LHVARWGRGSGRKLFAFMVLLGALVSALFANDGAALILTPIVISMLLALRFSPAATLAFVMGAGFIADTASLPLVVSNLV
NIVSADFFHIGFNRYAAVMVPVNFVSVAATLGMLLWFFRRDIPKAYDPEQLEHPETAIHDKATFYAGWAVLVILLIGCFA
LEPLGIPISAISAVCAALLLAIAARGHKISTRKVMKEAPWQIVIFSLGMYLVVYGLRNAGLTDYLAGWLNVFAGHGVWGA
AMGTGVLTALLSSIMNNLPTVLVGLLSIDASQATGVVKEAMIYANVIGSDLGPKITPIGSLATLLWLHVLERKDIRIGWG
YYFKVGIVLTVPVLLVTLAALALRLSV
>Mature_427_residues
MLAASLIFLLTITLVIWQPKGLGVGWSATFGAIVALIFGVVHLSDIPLVWQIIWNATGTFVALIIISLLLDEAGFFAWAA
LHVARWGRGSGRKLFAFMVLLGALVSALFANDGAALILTPIVISMLLALRFSPAATLAFVMGAGFIADTASLPLVVSNLV
NIVSADFFHIGFNRYAAVMVPVNFVSVAATLGMLLWFFRRDIPKAYDPEQLEHPETAIHDKATFYAGWAVLVILLIGCFA
LEPLGIPISAISAVCAALLLAIAARGHKISTRKVMKEAPWQIVIFSLGMYLVVYGLRNAGLTDYLAGWLNVFAGHGVWGA
AMGTGVLTALLSSIMNNLPTVLVGLLSIDASQATGVVKEAMIYANVIGSDLGPKITPIGSLATLLWLHVLERKDIRIGWG
YYFKVGIVLTVPVLLVTLAALALRLSV

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=426, Percent_Identity=71.830985915493, Blast_Score=589, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45621; Mature: 45621

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAASLIFLLTITLVIWQPKGLGVGWSATFGAIVALIFGVVHLSDIPLVWQIIWNATGTF
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
VALIIISLLLDEAGFFAWAALHVARWGRGSGRKLFAFMVLLGALVSALFANDGAALILTP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVISMLLALRFSPAATLAFVMGAGFIADTASLPLVVSNLVNIVSADFFHIGFNRYAAVMV
HHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEE
PVNFVSVAATLGMLLWFFRRDIPKAYDPEQLEHPETAIHDKATFYAGWAVLVILLIGCFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
LEPLGIPISAISAVCAALLLAIAARGHKISTRKVMKEAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LTDYLAGWLNVFAGHGVWGAAMGTGVLTALLSSIMNNLPTVLVGLLSIDASQATGVVKEA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MIYANVIGSDLGPKITPIGSLATLLWLHVLERKDIRIGWGYYFKVGIVLTVPVLLVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHH
LALRLSV
HHHHHCC
>Mature Secondary Structure
MLAASLIFLLTITLVIWQPKGLGVGWSATFGAIVALIFGVVHLSDIPLVWQIIWNATGTF
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
VALIIISLLLDEAGFFAWAALHVARWGRGSGRKLFAFMVLLGALVSALFANDGAALILTP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVISMLLALRFSPAATLAFVMGAGFIADTASLPLVVSNLVNIVSADFFHIGFNRYAAVMV
HHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEE
PVNFVSVAATLGMLLWFFRRDIPKAYDPEQLEHPETAIHDKATFYAGWAVLVILLIGCFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
LEPLGIPISAISAVCAALLLAIAARGHKISTRKVMKEAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LTDYLAGWLNVFAGHGVWGAAMGTGVLTALLSSIMNNLPTVLVGLLSIDASQATGVVKEA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MIYANVIGSDLGPKITPIGSLATLLWLHVLERKDIRIGWGYYFKVGIVLTVPVLLVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHH
LALRLSV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]