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The map label for this gene is ppnK [H]

Identifier: 77458422

GI number: 77458422

Start: 2478809

End: 2479699

Strand: Direct

Name: ppnK [H]

Synonym: Pfl01_2195

Alternate gene names: 77458422

Gene position: 2478809-2479699 (Clockwise)

Preceding gene: 77458420

Following gene: 77458423

Centisome position: 38.5

GC content: 60.72

Gene sequence:

>891_bases
ATGGAGCAATTTCGTAATATCGGCATCATCGGTCGCCTGGGCAGCTCCCAGGTGCTGGATACCGTCCGCCGACTGAAACG
CTTCCTGCTCGATCGTCACCTGCATGTGATTCTCGAAGACACCATCGCCGAAGTCCTGCCGGGCCATGGCCTGCAAACTT
CTTCACGCAAGATGCTCGGCGAAGTTTGCGACATGGTCATCGTGGTCGGCGGTGACGGCAGCCTGCTCGGCGCAGCGCGG
GCACTGGCCAAACACAATATTCCGGTGCTCGGGATCAACCGTGGCAGCCTCGGTTTTCTCACCGACATCCGCCCCGACGA
GCTGGAAATCAAGGTCGCCGAAGTGCTCGACGGCCATTATCTGGTGGAAAACCGCTTCCTGCTGCAGGCCGAAGTCCGTC
GTCACGCCGAGGCCATCGGCCAGGGCGATGCGCTGAACGACGTGGTGCTGCACCCCGGCAAATCGACGCGGATGATCGAG
TTCGAGCTGTACATCGACGGCCAGTTCGTCTGCAGCCAGAAGGCCGACGGCCTGATCGTCGCCACCCCGACCGGTTCCAC
CGCTTACGCACTGTCCGCCGGTGGCCCGATCATGCATCCCAAGCTTGACGCTATTGTGATTGTGCCGATGTACCCCCATA
CCTTGTCGGGCAGACCGATCGTGGTCGATGGCAATAGTGAGCTGAAAATCGTCGTGTCCAAAGATATGCAGATCTACCCG
CAGGTCTCCTGCGACGGGCAGAACCACTTCACCTGCGCGCCGGGCGACACCATCACCGTCAGCAAGAAAGCGCAGAAGCT
GCGGCTGATTCATCCGCTCGACCACAACTACTACGAAGTCTGCCGGACCAAGCTCGGCTGGGGCAGCAAGTTGGGTGGTG
GAGGCGACTGA

Upstream 100 bases:

>100_bases
TCAAACACCGGTGCATCCTCGGGGCTGAAAGGATTTTGTCTATCCGGCGCTTTCGCCCATAATCGTGCTTTTCGCCGTCT
CAGTCCCCCGCAGGAGTCCC

Downstream 100 bases:

>100_bases
TGCTCGATCCCGCGCGCAGTTATGACCTGATTGGTGACGTGCACGGATGCGCCCTGACCCTGGAACACCTGCTGGACCGT
CTCGGTTATCACAAGCAGGG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MEQFRNIGIIGRLGSSQVLDTVRRLKRFLLDRHLHVILEDTIAEVLPGHGLQTSSRKMLGEVCDMVIVVGGDGSLLGAAR
ALAKHNIPVLGINRGSLGFLTDIRPDELEIKVAEVLDGHYLVENRFLLQAEVRRHAEAIGQGDALNDVVLHPGKSTRMIE
FELYIDGQFVCSQKADGLIVATPTGSTAYALSAGGPIMHPKLDAIVIVPMYPHTLSGRPIVVDGNSELKIVVSKDMQIYP
QVSCDGQNHFTCAPGDTITVSKKAQKLRLIHPLDHNYYEVCRTKLGWGSKLGGGGD

Sequences:

>Translated_296_residues
MEQFRNIGIIGRLGSSQVLDTVRRLKRFLLDRHLHVILEDTIAEVLPGHGLQTSSRKMLGEVCDMVIVVGGDGSLLGAAR
ALAKHNIPVLGINRGSLGFLTDIRPDELEIKVAEVLDGHYLVENRFLLQAEVRRHAEAIGQGDALNDVVLHPGKSTRMIE
FELYIDGQFVCSQKADGLIVATPTGSTAYALSAGGPIMHPKLDAIVIVPMYPHTLSGRPIVVDGNSELKIVVSKDMQIYP
QVSCDGQNHFTCAPGDTITVSKKAQKLRLIHPLDHNYYEVCRTKLGWGSKLGGGGD
>Mature_296_residues
MEQFRNIGIIGRLGSSQVLDTVRRLKRFLLDRHLHVILEDTIAEVLPGHGLQTSSRKMLGEVCDMVIVVGGDGSLLGAAR
ALAKHNIPVLGINRGSLGFLTDIRPDELEIKVAEVLDGHYLVENRFLLQAEVRRHAEAIGQGDALNDVVLHPGKSTRMIE
FELYIDGQFVCSQKADGLIVATPTGSTAYALSAGGPIMHPKLDAIVIVPMYPHTLSGRPIVVDGNSELKIVVSKDMQIYP
QVSCDGQNHFTCAPGDTITVSKKAQKLRLIHPLDHNYYEVCRTKLGWGSKLGGGGD

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=325, Percent_Identity=29.2307692307692, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1788968, Length=292, Percent_Identity=47.2602739726027, Blast_Score=273, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6320794, Length=242, Percent_Identity=30.5785123966942, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=31.25, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325068, Length=219, Percent_Identity=31.9634703196347, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28573830, Length=224, Percent_Identity=38.8392857142857, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28573828, Length=255, Percent_Identity=35.6862745098039, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI28573826, Length=255, Percent_Identity=35.6862745098039, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161077047, Length=224, Percent_Identity=38.8392857142857, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI28573832, Length=224, Percent_Identity=38.8392857142857, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI20129957, Length=278, Percent_Identity=31.6546762589928, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24653422, Length=278, Percent_Identity=31.6546762589928, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI281363321, Length=280, Percent_Identity=31.4285714285714, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24653424, Length=280, Percent_Identity=31.4285714285714, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI281363323, Length=280, Percent_Identity=31.4285714285714, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 32213; Mature: 32213

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQFRNIGIIGRLGSSQVLDTVRRLKRFLLDRHLHVILEDTIAEVLPGHGLQTSSRKMLG
CCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EVCDMVIVVGGDGSLLGAARALAKHNIPVLGINRGSLGFLTDIRPDELEIKVAEVLDGHY
HHCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCEEEEEEHHHCCEE
LVENRFLLQAEVRRHAEAIGQGDALNDVVLHPGKSTRMIEFELYIDGQFVCSQKADGLIV
EEECEEEEHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEEEECCEEEECCCCCCEEE
ATPTGSTAYALSAGGPIMHPKLDAIVIVPMYPHTLSGRPIVVDGNSELKIVVSKDMQIYP
ECCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCEEEE
QVSCDGQNHFTCAPGDTITVSKKAQKLRLIHPLDHNYYEVCRTKLGWGSKLGGGGD
EEEECCCCCEEECCCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEQFRNIGIIGRLGSSQVLDTVRRLKRFLLDRHLHVILEDTIAEVLPGHGLQTSSRKMLG
CCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EVCDMVIVVGGDGSLLGAARALAKHNIPVLGINRGSLGFLTDIRPDELEIKVAEVLDGHY
HHCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCEEEEEEHHHCCEE
LVENRFLLQAEVRRHAEAIGQGDALNDVVLHPGKSTRMIEFELYIDGQFVCSQKADGLIV
EEECEEEEHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEEEECCEEEECCCCCCEEE
ATPTGSTAYALSAGGPIMHPKLDAIVIVPMYPHTLSGRPIVVDGNSELKIVVSKDMQIYP
ECCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCEEEE
QVSCDGQNHFTCAPGDTITVSKKAQKLRLIHPLDHNYYEVCRTKLGWGSKLGGGGD
EEEECCCCCEEECCCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA