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The map label for this gene is gloA [C]

Identifier: 77458386

GI number: 77458386

Start: 2441832

End: 2442245

Strand: Direct

Name: gloA [C]

Synonym: Pfl01_2159

Alternate gene names: 77458386

Gene position: 2441832-2442245 (Clockwise)

Preceding gene: 77458382

Following gene: 77458388

Centisome position: 37.93

GC content: 61.84

Gene sequence:

>414_bases
ATGCCACCGTTCACGATTCACCACATCGATCACATCGTGCTGCGCGTCGCCGATCTGCAACGCAGCGTTGATTTCTATGA
CCGGGTCTTCGGTGCCGAAGTGGTCAAGCGCAACGAAAAGTTCGGTCTGGTGCACCTGCGCGCCGGCACGTCGATGATCG
ATCTGGTCGACCTCGACGGCGAAATCGGCCGCAAGGGCGGCGCCGCTGCCGGCAGCGAGCGGCGCAATGTCGATCACTTC
TGTCTGCGCATCGAGCCGTTCGATGAAGCGGCGCTGATCAGTCATTTGCAATCCTGCGGCCTGACCGTCGACAAGGCCGC
CACCCGTTTCGGCGCCGAGGGCAACGGCCTGTCGTTGTACTGCTTTGATCCGGACGGCAATCAGGTCGAACTCAAAGGCC
CGTCCGAAGCCTAG

Upstream 100 bases:

>100_bases
ATAAAACCAGTTATTCGATGGCTCCACCGAAAAATCGACAGTGAATTTTCGGGCCGCTGCACTACAGTGATCGGGTCGCT
ACAACTCGGGAAATGAAGGT

Downstream 100 bases:

>100_bases
GCCCGCTTGGCCATCAGCAACACCGAAGCCGCCGCCGACAGCAGCCCGGCGCAGCAACGGTTGAAAATCCGCTTGCCGCG
CGGCTCGGCGAACCAGCGGC

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: (R)-S-Lactoylglutathione [C]

Alternate protein names: Lactoylglutathione Lyase; Ring-Cleaving Dioxygenase; Glyoxalase Family Protein; Methylmalonyl CoA Epimerase; Dioxygenase Protein; Glyoxalase; Glyoxalase/Bleomycin Resistance; Glutathione Transferase; Dioxygenase; Lactoylglutathione Lyase Family Protein

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MPPFTIHHIDHIVLRVADLQRSVDFYDRVFGAEVVKRNEKFGLVHLRAGTSMIDLVDLDGEIGRKGGAAAGSERRNVDHF
CLRIEPFDEAALISHLQSCGLTVDKAATRFGAEGNGLSLYCFDPDGNQVELKGPSEA

Sequences:

>Translated_137_residues
MPPFTIHHIDHIVLRVADLQRSVDFYDRVFGAEVVKRNEKFGLVHLRAGTSMIDLVDLDGEIGRKGGAAAGSERRNVDHF
CLRIEPFDEAALISHLQSCGLTVDKAATRFGAEGNGLSLYCFDPDGNQVELKGPSEA
>Mature_136_residues
PPFTIHHIDHIVLRVADLQRSVDFYDRVFGAEVVKRNEKFGLVHLRAGTSMIDLVDLDGEIGRKGGAAAGSERRNVDHFC
LRIEPFDEAALISHLQSCGLTVDKAATRFGAEGNGLSLYCFDPDGNQVELKGPSEA

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.4.1.5 [C]

Molecular weight: Translated: 15008; Mature: 14877

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPFTIHHIDHIVLRVADLQRSVDFYDRVFGAEVVKRNEKFGLVHLRAGTSMIDLVDLDG
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCC
EIGRKGGAAAGSERRNVDHFCLRIEPFDEAALISHLQSCGLTVDKAATRFGAEGNGLSLY
CCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEE
CFDPDGNQVELKGPSEA
EECCCCCEEEECCCCCC
>Mature Secondary Structure 
PPFTIHHIDHIVLRVADLQRSVDFYDRVFGAEVVKRNEKFGLVHLRAGTSMIDLVDLDG
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCC
EIGRKGGAAAGSERRNVDHFCLRIEPFDEAALISHLQSCGLTVDKAATRFGAEGNGLSLY
CCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEE
CFDPDGNQVELKGPSEA
EECCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: Reduced glutathione; Methylglyoxal [C]

Specific reaction: Reduced glutathione + Methylglyoxal --> (R)-S-Lactoylglutathione [C]

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA