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The map label for this gene is prpB [C]

Identifier: 77458384

GI number: 77458384

Start: 2439820

End: 2440617

Strand: Reverse

Name: prpB [C]

Synonym: Pfl01_2157

Alternate gene names: 77458384

Gene position: 2440617-2439820 (Counterclockwise)

Preceding gene: 77458385

Following gene: 77458383

Centisome position: 37.91

GC content: 62.78

Gene sequence:

>798_bases
ATGAACGCGCTCGACACTCAGTTTCACCAGTTGCACCACGATGACCTGCTGATCCTGACCAACGTTGCCGACGCCACCGG
CGCGCGACTGGTCGAGCAACTGGGTGGCAAAGCGGTCGCCACCAGCAGCGCCGCTGTCGCGTGGGCCCACGGTTACCCGG
ACGGCAACACCCTGCCGCTCGAGCGGTTGATCAGCACCGTGGAATCGATTGCCCGGGTCATCAAGGTGCCGTTGAGCGTG
GACATCGAAGCCGGTTATTCCGATGACCTCGACCGGGTCGCCGAAGTGATCGATGCGGTTATCGCAGCCGGCGCGGTCGG
GATCAACATCGAAGATGGCAGCGGCACGCCGGAACTGCTGGTGCGCAAGATCAAGGTCGCGCGGCAAGTGGCCGAGAAGC
GCAATGTGAAGCTGTTCATCAACGCCCGCACCGACGTTTACCTCAAAGGTCTGGTGCCGGCCGAGGATCGTGTCGCCGAA
ACCCTGCGTCGCGCGGCGCTTTATCAGGCAGCCGGGGCCGATGGGTTGTTCGCTGCCGGGGTGACGTCCGCCTACGAAAT
CGAAGCCATCTGCAAAGGTACGACCCTGCCGGTCAACGTCCTGGGATTCGCTTGCCTGCCCTCCCCCGAAGAACTGCAAA
CCCTCGGCGTGCGCCGCTTGAGCGTCGGTTCCGGCATCGCCGAGTTCCTGTACGGCGCCATGGGCTCACTGGTGAAAAGT
TTTCTCGCCAGCGGCAAGCTCGACACCCATGACCTCAAGGCCTTCACCTATGGCGAAGTCAACGGTCTGCTGAAGTAA

Upstream 100 bases:

>100_bases
GGTGGGCAACCGCGCAAGGCGTTTTTGCTCCGGCTGGAGAACGCCGCCGTGCAGCTCACGCAACAACTGGCTTTCTGACC
AACGACAAGAAGGGATTTCG

Downstream 100 bases:

>100_bases
CTCATGCCCGATCCCTACCAACCGGCCAGCGAGTTTCTGGCCAGCCTCGATGCCGACTGGCAGCGCCACATTGCTGCGGT
CGGTCCCTGCCTGCATCAGC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNALDTQFHQLHHDDLLILTNVADATGARLVEQLGGKAVATSSAAVAWAHGYPDGNTLPLERLISTVESIARVIKVPLSV
DIEAGYSDDLDRVAEVIDAVIAAGAVGINIEDGSGTPELLVRKIKVARQVAEKRNVKLFINARTDVYLKGLVPAEDRVAE
TLRRAALYQAAGADGLFAAGVTSAYEIEAICKGTTLPVNVLGFACLPSPEELQTLGVRRLSVGSGIAEFLYGAMGSLVKS
FLASGKLDTHDLKAFTYGEVNGLLK

Sequences:

>Translated_265_residues
MNALDTQFHQLHHDDLLILTNVADATGARLVEQLGGKAVATSSAAVAWAHGYPDGNTLPLERLISTVESIARVIKVPLSV
DIEAGYSDDLDRVAEVIDAVIAAGAVGINIEDGSGTPELLVRKIKVARQVAEKRNVKLFINARTDVYLKGLVPAEDRVAE
TLRRAALYQAAGADGLFAAGVTSAYEIEAICKGTTLPVNVLGFACLPSPEELQTLGVRRLSVGSGIAEFLYGAMGSLVKS
FLASGKLDTHDLKAFTYGEVNGLLK
>Mature_265_residues
MNALDTQFHQLHHDDLLILTNVADATGARLVEQLGGKAVATSSAAVAWAHGYPDGNTLPLERLISTVESIARVIKVPLSV
DIEAGYSDDLDRVAEVIDAVIAAGAVGINIEDGSGTPELLVRKIKVARQVAEKRNVKLFINARTDVYLKGLVPAEDRVAE
TLRRAALYQAAGADGLFAAGVTSAYEIEAICKGTTLPVNVLGFACLPSPEELQTLGVRRLSVGSGIAEFLYGAMGSLVKS
FLASGKLDTHDLKAFTYGEVNGLLK

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28025; Mature: 28025

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALDTQFHQLHHDDLLILTNVADATGARLVEQLGGKAVATSSAAVAWAHGYPDGNTLPL
CCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCH
ERLISTVESIARVIKVPLSVDIEAGYSDDLDRVAEVIDAVIAAGAVGINIEDGSGTPELL
HHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHH
VRKIKVARQVAEKRNVKLFINARTDVYLKGLVPAEDRVAETLRRAALYQAAGADGLFAAG
HHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHC
VTSAYEIEAICKGTTLPVNVLGFACLPSPEELQTLGVRRLSVGSGIAEFLYGAMGSLVKS
CCCHHHHHHHHCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FLASGKLDTHDLKAFTYGEVNGLLK
HHHCCCCCHHHCCEEEECCCCCCCC
>Mature Secondary Structure
MNALDTQFHQLHHDDLLILTNVADATGARLVEQLGGKAVATSSAAVAWAHGYPDGNTLPL
CCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCH
ERLISTVESIARVIKVPLSVDIEAGYSDDLDRVAEVIDAVIAAGAVGINIEDGSGTPELL
HHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHH
VRKIKVARQVAEKRNVKLFINARTDVYLKGLVPAEDRVAETLRRAALYQAAGADGLFAAG
HHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHC
VTSAYEIEAICKGTTLPVNVLGFACLPSPEELQTLGVRRLSVGSGIAEFLYGAMGSLVKS
CCCHHHHHHHHCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FLASGKLDTHDLKAFTYGEVNGLLK
HHHCCCCCHHHCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]