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The map label for this gene is potF [H]

Identifier: 77458344

GI number: 77458344

Start: 2402355

End: 2403452

Strand: Reverse

Name: potF [H]

Synonym: Pfl01_2117

Alternate gene names: 77458344

Gene position: 2403452-2402355 (Counterclockwise)

Preceding gene: 77458345

Following gene: 77458342

Centisome position: 37.33

GC content: 59.47

Gene sequence:

>1098_bases
ATGAAAATGTTTGGCAGGACTCTGCTGACACTGTCCTTATTGGGCGCAATCGCCACAGGCGCCCAGGCCAACGACAAGGT
GCTGCGCGTTTACAACTGGTCGGATTACATCGCCCCGGACACCGTCAAGAAGTTCGAGGACGAGACCGGCATCCGCGTGA
CCTACGACGTGTTCGACAGCAATGAAACCCTCGAGGCGCGTCTGCTCGCGGGTAAATCCGGCTACGACATCGTGGTGCCG
TCCAACAGTTTCCTGGCCAAGCAGATCAAGGCCGGGGTCTATCAGACCCTGGACAAATCGAAACTGCCAAACTGGAAAAA
CCTCAACCCGGTGCTGCTGAAAAACGCCTCCGCCAGTGATCCGGACAACGCCCACGCGTTCCCGTACATGTGGGGTTCGA
TCGGCATCGGCTTCAACCCGGACAAGGTCAAGGAAGTGCTCGGCAAGAATGCGCCGACCAATTCCTGGGACCTGCTGTTC
AAACCGGAGAACGCCGAGAAGCTCAAGGCCTGCGGTATCAGCTTCCTCGACTCGCCCACCGAAATGATCCCGGCCGCCCT
GCACTACCTGGGCTATCCGGTGAGCGACAAGGACGCCGCGCACATCAAGGAAGCCGAGGCGCTGTTCATGAAGATTCGCC
CGAACGTGGCGTATTTCCACTCTTCGAAATACATTTCGGACCTGGCCAACGGCAACATCTGCGTGGCAGTCGGTTACTCC
GGTGATGTATTGCAGGCCAAGGCCCGCGCGGTGGAATCGGGCAACAACGTCGTGATCGACTACAGCATTCCCAAGGAAGG
TGCCGGCAGCTTCTACGACATGGTCGCCATCCCGCGCGATGCAGCGAACGTCGAGAACGCCTACCTGTTCATGGACTTCC
TGATGCGCCCGGACATCATCGCCGAGATCACCAACAGCAACGGCTACAGCAACGCCAACGCAGCGGCGACGCCGCTGGTG
GACGAAGCGATCCGCAACGATCCGGGCTCGTACCCGTCGCAGGAAGTGATGGCGACACTGTATGCGGTGCCGGATCAACC
GATTGCCACCCAGCGGATCATGACCCGGGGCTGGACCCGCGTGAAACTCGGCAAATAA

Upstream 100 bases:

>100_bases
GCAACCTCAACGAGTAGCAGCGACGGCCTAAGGCCGTCGCGTTTTACGGCTTTGCGCTTTTGCTTAAAAGCTTCTGAATA
AAAAGAATGGAGTTTCACCG

Downstream 100 bases:

>100_bases
ACACACATCGATTTCCGTTCATGCAGCGCCTTACCACCGCTGCCCCCTGCTCTGAACGGCCTCACCTGGCTCTCCTCGGT
TCAGGTGAATTCAGGCGCCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKMFGRTLLTLSLLGAIATGAQANDKVLRVYNWSDYIAPDTVKKFEDETGIRVTYDVFDSNETLEARLLAGKSGYDIVVP
SNSFLAKQIKAGVYQTLDKSKLPNWKNLNPVLLKNASASDPDNAHAFPYMWGSIGIGFNPDKVKEVLGKNAPTNSWDLLF
KPENAEKLKACGISFLDSPTEMIPAALHYLGYPVSDKDAAHIKEAEALFMKIRPNVAYFHSSKYISDLANGNICVAVGYS
GDVLQAKARAVESGNNVVIDYSIPKEGAGSFYDMVAIPRDAANVENAYLFMDFLMRPDIIAEITNSNGYSNANAAATPLV
DEAIRNDPGSYPSQEVMATLYAVPDQPIATQRIMTRGWTRVKLGK

Sequences:

>Translated_365_residues
MKMFGRTLLTLSLLGAIATGAQANDKVLRVYNWSDYIAPDTVKKFEDETGIRVTYDVFDSNETLEARLLAGKSGYDIVVP
SNSFLAKQIKAGVYQTLDKSKLPNWKNLNPVLLKNASASDPDNAHAFPYMWGSIGIGFNPDKVKEVLGKNAPTNSWDLLF
KPENAEKLKACGISFLDSPTEMIPAALHYLGYPVSDKDAAHIKEAEALFMKIRPNVAYFHSSKYISDLANGNICVAVGYS
GDVLQAKARAVESGNNVVIDYSIPKEGAGSFYDMVAIPRDAANVENAYLFMDFLMRPDIIAEITNSNGYSNANAAATPLV
DEAIRNDPGSYPSQEVMATLYAVPDQPIATQRIMTRGWTRVKLGK
>Mature_365_residues
MKMFGRTLLTLSLLGAIATGAQANDKVLRVYNWSDYIAPDTVKKFEDETGIRVTYDVFDSNETLEARLLAGKSGYDIVVP
SNSFLAKQIKAGVYQTLDKSKLPNWKNLNPVLLKNASASDPDNAHAFPYMWGSIGIGFNPDKVKEVLGKNAPTNSWDLLF
KPENAEKLKACGISFLDSPTEMIPAALHYLGYPVSDKDAAHIKEAEALFMKIRPNVAYFHSSKYISDLANGNICVAVGYS
GDVLQAKARAVESGNNVVIDYSIPKEGAGSFYDMVAIPRDAANVENAYLFMDFLMRPDIIAEITNSNGYSNANAAATPLV
DEAIRNDPGSYPSQEVMATLYAVPDQPIATQRIMTRGWTRVKLGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=359, Percent_Identity=56.2674094707521, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787367, Length=339, Percent_Identity=34.8082595870207, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1787711, Length=356, Percent_Identity=23.314606741573, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39969; Mature: 39969

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMFGRTLLTLSLLGAIATGAQANDKVLRVYNWSDYIAPDTVKKFEDETGIRVTYDVFDS
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEEEEEECC
NETLEARLLAGKSGYDIVVPSNSFLAKQIKAGVYQTLDKSKLPNWKNLNPVLLKNASASD
CCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCC
PDNAHAFPYMWGSIGIGFNPDKVKEVLGKNAPTNSWDLLFKPENAEKLKACGISFLDSPT
CCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCCHHCCCCH
EMIPAALHYLGYPVSDKDAAHIKEAEALFMKIRPNVAYFHSSKYISDLANGNICVAVGYS
HHHHHHHHHHCCCCCCCCHHHHHHHHEEEEEECCCEEEEECCHHHHHHCCCCEEEEECCC
GDVLQAKARAVESGNNVVIDYSIPKEGAGSFYDMVAIPRDAANVENAYLFMDFLMRPDII
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEE
AEITNSNGYSNANAAATPLVDEAIRNDPGSYPSQEVMATLYAVPDQPIATQRIMTRGWTR
EEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCE
VKLGK
EECCC
>Mature Secondary Structure
MKMFGRTLLTLSLLGAIATGAQANDKVLRVYNWSDYIAPDTVKKFEDETGIRVTYDVFDS
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEEEEEECC
NETLEARLLAGKSGYDIVVPSNSFLAKQIKAGVYQTLDKSKLPNWKNLNPVLLKNASASD
CCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCC
PDNAHAFPYMWGSIGIGFNPDKVKEVLGKNAPTNSWDLLFKPENAEKLKACGISFLDSPT
CCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCCHHCCCCH
EMIPAALHYLGYPVSDKDAAHIKEAEALFMKIRPNVAYFHSSKYISDLANGNICVAVGYS
HHHHHHHHHHCCCCCCCCHHHHHHHHEEEEEECCCEEEEECCHHHHHHCCCCEEEEECCC
GDVLQAKARAVESGNNVVIDYSIPKEGAGSFYDMVAIPRDAANVENAYLFMDFLMRPDII
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEE
AEITNSNGYSNANAAATPLVDEAIRNDPGSYPSQEVMATLYAVPDQPIATQRIMTRGWTR
EEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCE
VKLGK
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]