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The map label for this gene is potI [H]

Identifier: 77458345

GI number: 77458345

Start: 2403522

End: 2404406

Strand: Reverse

Name: potI [H]

Synonym: Pfl01_2118

Alternate gene names: 77458345

Gene position: 2404406-2403522 (Counterclockwise)

Preceding gene: 77458346

Following gene: 77458344

Centisome position: 37.34

GC content: 61.47

Gene sequence:

>885_bases
ATGAAACGCTTCGGATTTTCAAAGTTCATGCTGATCTTCGGCCTGATGTTCATCTATCTGCCGATGCTGATTCTGGTGAT
CTACTCGTTCAACGCCTCGAAACTGGTGACGGTCTGGGGCGGCTGGTCGGTGAAGTGGTACGTCGGCCTGCTCGACAACA
CGCAACTGATGGGCTCGGTGGTGCGCTCGCTGGAAATCGCCTGCTACACCGCGATTGCCGCCGTAGCGTTGGGCACCCTC
GCTGCGTTCGTGCTGACCCGCGTGACCCGCTTCAAGGGTCGTACGCTGTTCGGTGGTCTGGTGACCGCGCCGCTGGTGAT
GCCTGAAGTGATCACGGGTCTGTCGCTGTTGCTGCTGTTCGTGGCGATGGCGCAACTGATCGGCTGGCCGCAGGAGCGCG
GCATCGTCACCATCTGGATCGCCCACACCACGTTTTGTGCCGCTTATGTGGCGGTGGTAGTCTCCGCCCGCTTGCGCGAG
CTGGACCTGTCGATCGAAGAAGCGGCGATGGATCTGGGCGCGAAACCGTTCAAAGTGTTTTTCCTGATCACCATCCCGAT
GATCGCGCCGTCGCTGGCGGCGGGCGGCATGATGTCGTTCGCCCTGTCGCTGGATGACCTGGTGCTGGCGAGCTTCGTGT
CGGGCCCTGGCTCCACCACCCTGCCGATGGAAGTCTTCTCGGCCGTGCGTCTGGGTGTGAAGCCCGAGATTAACGCTGTG
GCCAGCCTGATTCTGCTGGCGGTGTCGCTGGTGACCTTCCTGGTCTGGTACTTCGGCCGCAAGGCGGAAGCCAACCGCAA
GCGGGCGATTCAGGAAGCGATGGATCAGACCGCCAACGAATCGTGGCAGCAACCGCAACCTCAACGAGTAGCAGCGACGG
CCTAA

Upstream 100 bases:

>100_bases
GGCCGGTGGCGTCTGCTCTGGCGGTGGTGATGCTGTTGATCCTGATTGTGCCGATCCTGCTGTTCAACCGCAGCCAGGCC
AAAGAGATGGAGGGACGAGG

Downstream 100 bases:

>100_bases
GGCCGTCGCGTTTTACGGCTTTGCGCTTTTGCTTAAAAGCTTCTGAATAAAAAGAATGGAGTTTCACCGATGAAAATGTT
TGGCAGGACTCTGCTGACAC

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKRFGFSKFMLIFGLMFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNTQLMGSVVRSLEIACYTAIAAVALGTL
AAFVLTRVTRFKGRTLFGGLVTAPLVMPEVITGLSLLLLFVAMAQLIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRE
LDLSIEEAAMDLGAKPFKVFFLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
ASLILLAVSLVTFLVWYFGRKAEANRKRAIQEAMDQTANESWQQPQPQRVAATA

Sequences:

>Translated_294_residues
MKRFGFSKFMLIFGLMFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNTQLMGSVVRSLEIACYTAIAAVALGTL
AAFVLTRVTRFKGRTLFGGLVTAPLVMPEVITGLSLLLLFVAMAQLIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRE
LDLSIEEAAMDLGAKPFKVFFLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
ASLILLAVSLVTFLVWYFGRKAEANRKRAIQEAMDQTANESWQQPQPQRVAATA
>Mature_294_residues
MKRFGFSKFMLIFGLMFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNTQLMGSVVRSLEIACYTAIAAVALGTL
AAFVLTRVTRFKGRTLFGGLVTAPLVMPEVITGLSLLLLFVAMAQLIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRE
LDLSIEEAAMDLGAKPFKVFFLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
ASLILLAVSLVTFLVWYFGRKAEANRKRAIQEAMDQTANESWQQPQPQRVAATA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=264, Percent_Identity=64.3939393939394, Blast_Score=310, Evalue=6e-86,
Organism=Escherichia coli, GI1787368, Length=226, Percent_Identity=38.9380530973451, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=32.03125, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=29.2452830188679, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32057; Mature: 32057

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFGFSKFMLIFGLMFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNTQLMGSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCHHHHHHH
VRSLEIACYTAIAAVALGTLAAFVLTRVTRFKGRTLFGGLVTAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAMAQLIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRELDLSIEEAAMDLGAKPFKVF
HHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
FLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHH
ASLILLAVSLVTFLVWYFGRKAEANRKRAIQEAMDQTANESWQQPQPQRVAATA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKRFGFSKFMLIFGLMFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNTQLMGSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCHHHHHHH
VRSLEIACYTAIAAVALGTLAAFVLTRVTRFKGRTLFGGLVTAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAMAQLIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRELDLSIEEAAMDLGAKPFKVF
HHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
FLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHH
ASLILLAVSLVTFLVWYFGRKAEANRKRAIQEAMDQTANESWQQPQPQRVAATA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]