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The map label for this gene is ligT [H]

Identifier: 77458343

GI number: 77458343

Start: 2401694

End: 2402233

Strand: Direct

Name: ligT [H]

Synonym: Pfl01_2116

Alternate gene names: 77458343

Gene position: 2401694-2402233 (Clockwise)

Preceding gene: 77458341

Following gene: 77458349

Centisome position: 37.3

GC content: 65.93

Gene sequence:

>540_bases
ATGACGGACGAAACGCGCGGGGATCAACCGGTCAAGCGGCTGTTTTTCGCCCTGGACTGCCCGCCGGCGCAGCGCAAGGC
GATTGCCCAGTGGCGCAGCGAACTGGGTTTGCGTACCGGTAAACCGGTGCCGGCCGACAACTTTCACCTGACGCTGCTGT
TTCTCGGCGCGGTGCCGTTGGCGCAGATCGGCGAAGTCTGCGAGGCGGCGGCGAAGGTGCGAACACCGGGCGAGGTGCTG
AGGATTTCGCTGGATCGGTTGCAGGTCTGGCGGCGTGCCGGGGTGTTGTCGCTGGCGCCGGAGCAGGCCCCGCAGGCATT
GCTGCGACTGGTTTACGCGCTGGAGCAGGCGATGTTGCCATTCGGATTTGAAGAGGCGACGCGCGAGTATCGTCCACATC
TGACGCTGGCCCGGGACTATCGGGCGCCGGAGCCGGAATCTGCGACGCCGCCGGAGTTCTTCCTGCGGGCCGAACGCTTT
GCGCTGTTCGAATCCCACAAGGGTCGCTACCGCTCGCTGGCCGACTGGCCACTGATCTGA

Upstream 100 bases:

>100_bases
TCCGGTTGATGGCAATCGTTGAACTATAAACCCCATGCCGAGAGTCGCCAGAAACGGGCGTGTAGAATCCAGGGATCTCG
ACGAACGAAGGAGCGGGGCC

Downstream 100 bases:

>100_bases
GCCACAAAAAAAGGCGCCCGAGGGCGCCTGAATTCACCTGAACCGAGGAGAGCCAGGTGAGGCCGTTCAGAGCAGGGGGC
AGCGGTGGTAAGGCGCTGCA

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MTDETRGDQPVKRLFFALDCPPAQRKAIAQWRSELGLRTGKPVPADNFHLTLLFLGAVPLAQIGEVCEAAAKVRTPGEVL
RISLDRLQVWRRAGVLSLAPEQAPQALLRLVYALEQAMLPFGFEEATREYRPHLTLARDYRAPEPESATPPEFFLRAERF
ALFESHKGRYRSLADWPLI

Sequences:

>Translated_179_residues
MTDETRGDQPVKRLFFALDCPPAQRKAIAQWRSELGLRTGKPVPADNFHLTLLFLGAVPLAQIGEVCEAAAKVRTPGEVL
RISLDRLQVWRRAGVLSLAPEQAPQALLRLVYALEQAMLPFGFEEATREYRPHLTLARDYRAPEPESATPPEFFLRAERF
ALFESHKGRYRSLADWPLI
>Mature_178_residues
TDETRGDQPVKRLFFALDCPPAQRKAIAQWRSELGLRTGKPVPADNFHLTLLFLGAVPLAQIGEVCEAAAKVRTPGEVLR
ISLDRLQVWRRAGVLSLAPEQAPQALLRLVYALEQAMLPFGFEEATREYRPHLTLARDYRAPEPESATPPEFFLRAERFA
LFESHKGRYRSLADWPLI

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=177, Percent_Identity=36.1581920903955, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20279; Mature: 20147

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDETRGDQPVKRLFFALDCPPAQRKAIAQWRSELGLRTGKPVPADNFHLTLLFLGAVPL
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCH
AQIGEVCEAAAKVRTPGEVLRISLDRLQVWRRAGVLSLAPEQAPQALLRLVYALEQAMLP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
FGFEEATREYRPHLTLARDYRAPEPESATPPEFFLRAERFALFESHKGRYRSLADWPLI
CCHHHHHHHHCCCCEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDETRGDQPVKRLFFALDCPPAQRKAIAQWRSELGLRTGKPVPADNFHLTLLFLGAVPL
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCH
AQIGEVCEAAAKVRTPGEVLRISLDRLQVWRRAGVLSLAPEQAPQALLRLVYALEQAMLP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
FGFEEATREYRPHLTLARDYRAPEPESATPPEFFLRAERFALFESHKGRYRSLADWPLI
CCHHHHHHHHCCCCEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]