Click here to switch to the map view.

The map label for this gene is puuA [H]

Identifier: 77458349

GI number: 77458349

Start: 2407720

End: 2409096

Strand: Direct

Name: puuA [H]

Synonym: Pfl01_2122

Alternate gene names: 77458349

Gene position: 2407720-2409096 (Clockwise)

Preceding gene: 77458343

Following gene: 77458350

Centisome position: 37.4

GC content: 60.57

Gene sequence:

>1377_bases
ATGTCGGTCCCTCTGCGTGCCGTTCAACTCAACGAAGCAAACGCATTCCTTAAGAAACATCCTGAGGTTTTGTACGTCGA
CCTTCTGATTGCAGACATGAACGGTGTGGTGCGCGGCAAGCGCATCGAGCGCACCAGTCTTCACAAGGTTTACGAGAAAG
GCATCAACCTGCCGGCCTCGCTCTTTGCCCTCGACATCAACGGCTCCACGGTGGAAAGCACCGGCCTGGGCCTGGACATC
GGCGACGCCGACCGTATCTGCTACCCGATCCCTGGCACCCTGAGCATCGAGCCCTGGCAGAAGCGCCCGACCGCACAACT
GCTGATGACCATGCACGAACTGGAAGGCGAGCCGTTCTTTGCCGACCCGCGTGAAGTGCTGGCCAACGTGGTGCGCAAGT
TCGACGCATTGGGCCTGACCATTTGCGCCGCGTTCGAACTCGAGTTCTACCTGATTGACCAGGACAACGTGAACGGCCGT
CCGCAATCGCCGCGTTCGCCGGTTTCGGGCAAGCGTCCGGTGTCGACCCAGGTCTATCTGATCGACGACCTCGACGAGTA
TGTCGACTGCCTGCAAGACATCCTCGAAGGCGCGAAAGAGCAGGGCATTCCTGCCGACGCCATCGTCAAGGAAAGCGCCC
CGGCGCAATTCGAAGTCAACCTGCATCACGTGTCTGACCCGATCAAGGCGTGCGACTACGCCGTCCTGCTCAAGCGTCTG
GTGAAGAACATCGCCTACGACCACGAGATGGACACCACCTTCATGGCCAAGCCGTATCCGGGCCAGGCGGGCAACGGTCT
GCACGTGCACATTTCGATTCTGGACAAAGAAGGCAATAACATCTTCGCCAGCGAGGATCCCGAGCAGAACGCCGCGCTAC
GCCACGCGATCGGCGGTGTGCTGGAGACCCTACCTGCGCAAATGGCCTTCCTGTGCCCGAACGTCAACTCGTACCGCCGC
TTCGGCGCGCAGTTTTATGTGCCGAACTCGCCGAGCTGGGGTATCGACAACCGCACCGTGGCCGTCCGCGTTCCGACCGG
TTCGCCGGATGCCGTGCGCATCGAACACCGTGTCGCCGGTGCCGACGCCAACCCATACCTGCTGATGGCTTCCGTGCTGG
CCGGTATCCACCACGGCCTGACCAACGAAATCGAACCGGGCGCCCCGGTCGAAGGCAACAGCTACGAGCAGAACGAACAG
AGCCTGCCGAACAACCTGCGCGACGCCCTGCGTGAGCTGGACGACAGCGAAGTCATGGCCCGCTACATCGACCCGATGTA
CATCGACGTATTTGTCGCGTGCAAGGAAAGCGAGCTGGCCGAGTTCGAAAACTCGATCTCTGACCTTGAGTACAACTGGT
ATCTGCATACGGTGTGA

Upstream 100 bases:

>100_bases
AGCGGCCCTGTGTTCGCTATTGACTTCACTTTGCCGTTCGGGTTGACTGGCTCGGCGAAACAGCAGTGAACATAATAATT
AACAGCTAAATAGGTGCATC

Downstream 100 bases:

>100_bases
TTCTGAAAAGCCGCTCACCCTAACCCTCTCCCCGAGGGAGAGGGGACTGACCGAGTTGTCTCGGGATTTCCGGCGACCTG
ACCGATCCAATCGATTATGG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSVPLRAVQLNEANAFLKKHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDI
GDADRICYPIPGTLSIEPWQKRPTAQLLMTMHELEGEPFFADPREVLANVVRKFDALGLTICAAFELEFYLIDQDNVNGR
PQSPRSPVSGKRPVSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVSDPIKACDYAVLLKRL
VKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDKEGNNIFASEDPEQNAALRHAIGGVLETLPAQMAFLCPNVNSYRR
FGAQFYVPNSPSWGIDNRTVAVRVPTGSPDAVRIEHRVAGADANPYLLMASVLAGIHHGLTNEIEPGAPVEGNSYEQNEQ
SLPNNLRDALRELDDSEVMARYIDPMYIDVFVACKESELAEFENSISDLEYNWYLHTV

Sequences:

>Translated_458_residues
MSVPLRAVQLNEANAFLKKHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDI
GDADRICYPIPGTLSIEPWQKRPTAQLLMTMHELEGEPFFADPREVLANVVRKFDALGLTICAAFELEFYLIDQDNVNGR
PQSPRSPVSGKRPVSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVSDPIKACDYAVLLKRL
VKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDKEGNNIFASEDPEQNAALRHAIGGVLETLPAQMAFLCPNVNSYRR
FGAQFYVPNSPSWGIDNRTVAVRVPTGSPDAVRIEHRVAGADANPYLLMASVLAGIHHGLTNEIEPGAPVEGNSYEQNEQ
SLPNNLRDALRELDDSEVMARYIDPMYIDVFVACKESELAEFENSISDLEYNWYLHTV
>Mature_457_residues
SVPLRAVQLNEANAFLKKHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDIG
DADRICYPIPGTLSIEPWQKRPTAQLLMTMHELEGEPFFADPREVLANVVRKFDALGLTICAAFELEFYLIDQDNVNGRP
QSPRSPVSGKRPVSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVSDPIKACDYAVLLKRLV
KNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDKEGNNIFASEDPEQNAALRHAIGGVLETLPAQMAFLCPNVNSYRRF
GAQFYVPNSPSWGIDNRTVAVRVPTGSPDAVRIEHRVAGADANPYLLMASVLAGIHHGLTNEIEPGAPVEGNSYEQNEQS
LPNNLRDALRELDDSEVMARYIDPMYIDVFVACKESELAEFENSISDLEYNWYLHTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=441, Percent_Identity=23.3560090702948, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI87081870, Length=445, Percent_Identity=44.7191011235955, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1790301, Length=480, Percent_Identity=26.25, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50754; Mature: 50623

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPLRAVQLNEANAFLKKHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
CCCCCEEEEECCHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEE
LFALDINGSTVESTGLGLDIGDADRICYPIPGTLSIEPWQKRPTAQLLMTMHELEGEPFF
EEEEECCCCEEECCCCCEECCCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHCCCCCC
ADPREVLANVVRKFDALGLTICAAFELEFYLIDQDNVNGRPQSPRSPVSGKRPVSTQVYL
CCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEE
IDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVSDPIKACDYAVLLKRL
ECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHH
VKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDKEGNNIFASEDPEQNAALRHAIGGV
HHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHH
LETLPAQMAFLCPNVNSYRRFGAQFYVPNSPSWGIDNRTVAVRVPTGSPDAVRIEHRVAG
HHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECC
ADANPYLLMASVLAGIHHGLTNEIEPGAPVEGNSYEQNEQSLPNNLRDALRELDDSEVMA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCHHHHHH
RYIDPMYIDVFVACKESELAEFENSISDLEYNWYLHTV
HHCCCEEEEEEEEECCHHHHHHHHHHHCCEEEEEEEEC
>Mature Secondary Structure 
SVPLRAVQLNEANAFLKKHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
CCCCEEEEECCHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEE
LFALDINGSTVESTGLGLDIGDADRICYPIPGTLSIEPWQKRPTAQLLMTMHELEGEPFF
EEEEECCCCEEECCCCCEECCCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHCCCCCC
ADPREVLANVVRKFDALGLTICAAFELEFYLIDQDNVNGRPQSPRSPVSGKRPVSTQVYL
CCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEE
IDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVSDPIKACDYAVLLKRL
ECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHH
VKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDKEGNNIFASEDPEQNAALRHAIGGV
HHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHH
LETLPAQMAFLCPNVNSYRRFGAQFYVPNSPSWGIDNRTVAVRVPTGSPDAVRIEHRVAG
HHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECC
ADANPYLLMASVLAGIHHGLTNEIEPGAPVEGNSYEQNEQSLPNNLRDALRELDDSEVMA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCHHHHHH
RYIDPMYIDVFVACKESELAEFENSISDLEYNWYLHTV
HHCCCEEEEEEEEECCHHHHHHHHHHHCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]