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The map label for this gene is rutB [H]

Identifier: 77458285

GI number: 77458285

Start: 2345992

End: 2346636

Strand: Reverse

Name: rutB [H]

Synonym: Pfl01_2058

Alternate gene names: 77458285

Gene position: 2346636-2345992 (Counterclockwise)

Preceding gene: 77458286

Following gene: 77458284

Centisome position: 36.45

GC content: 64.81

Gene sequence:

>645_bases
ATGTTCAGCCTTCCCCATCGTTCGCCACGGGACCTGCCGTTTGCCACCGACCACACCGCCCTGTTGCTGGTGGACATGCA
GCGCGCCTGGCTCGAGCCACAGTTCGACCCGCACCTCGAAGGCCCGGACGCCGCGTACTTCCTCAACCGCACGCGCTCGC
AAGTGGTGCCCAATCAGGTTCGCCTGCTCAATGCCTTCCGCGACGCACGGCAGAACGTGCTGCACACCCTGATCGAAAGC
CTGACCGCCGACGGCCGCGATCGCTCGCTGGATCACAAACTGTCGGACATGCACCTGCCCAAGGGCAGCCCGCAGGCACA
GGTCATTGCCGAGCTGACGCCGGCGGAAAACGAAATCGTGCTGCCCAAGACCTCGTCCGGGGTCTTCAACTCGACCAACA
TCGACTATGTGCTGCGCAATCTGCAAACCCGTCACCTGATCATCGCCGGCATCGTCACCGACCAGTGCGTGGACATGGCC
GTGCGTGACGCCGCCGACCGTGGCTACCTCGTCACCCTGGTGGCGGACGCCTGCGCGACGTACACCGAAGCACGCCACGA
CGCCTGCCTGAACGCGGTCAAGGGCTACTGCTGGATCACCGACACCGACACCGTGCTCGGCCGGTTGCAGGAGATGCAGC
CATGA

Upstream 100 bases:

>100_bases
CCTGCCTGACCCTGCGCAGCCCGGACCTGGCCGATCGCCTGGAAGGCATCGATGCCCTGCGCAACGACTTCGACACCCAT
TTGCTGGAGGATAAATAAGG

Downstream 100 bases:

>100_bases
GCGAACGCCTGTCGCCGTTGCCGATGACCACCATCGTCACCACCGACCTGATCGGCGTTACCCGTGGCCGTTCGTTTCCC
ACCGATGAGCTGGAACACTA

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MFSLPHRSPRDLPFATDHTALLLVDMQRAWLEPQFDPHLEGPDAAYFLNRTRSQVVPNQVRLLNAFRDARQNVLHTLIES
LTADGRDRSLDHKLSDMHLPKGSPQAQVIAELTPAENEIVLPKTSSGVFNSTNIDYVLRNLQTRHLIIAGIVTDQCVDMA
VRDAADRGYLVTLVADACATYTEARHDACLNAVKGYCWITDTDTVLGRLQEMQP

Sequences:

>Translated_214_residues
MFSLPHRSPRDLPFATDHTALLLVDMQRAWLEPQFDPHLEGPDAAYFLNRTRSQVVPNQVRLLNAFRDARQNVLHTLIES
LTADGRDRSLDHKLSDMHLPKGSPQAQVIAELTPAENEIVLPKTSSGVFNSTNIDYVLRNLQTRHLIIAGIVTDQCVDMA
VRDAADRGYLVTLVADACATYTEARHDACLNAVKGYCWITDTDTVLGRLQEMQP
>Mature_214_residues
MFSLPHRSPRDLPFATDHTALLLVDMQRAWLEPQFDPHLEGPDAAYFLNRTRSQVVPNQVRLLNAFRDARQNVLHTLIES
LTADGRDRSLDHKLSDMHLPKGSPQAQVIAELTPAENEIVLPKTSSGVFNSTNIDYVLRNLQTRHLIIAGIVTDQCVDMA
VRDAADRGYLVTLVADACATYTEARHDACLNAVKGYCWITDTDTVLGRLQEMQP

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=214, Percent_Identity=28.9719626168224, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 23959; Mature: 23959

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSLPHRSPRDLPFATDHTALLLVDMQRAWLEPQFDPHLEGPDAAYFLNRTRSQVVPNQV
CCCCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCHHHH
RLLNAFRDARQNVLHTLIESLTADGRDRSLDHKLSDMHLPKGSPQAQVIAELTPAENEIV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHEEEEECCCCCCEE
LPKTSSGVFNSTNIDYVLRNLQTRHLIIAGIVTDQCVDMAVRDAADRGYLVTLVADACAT
EECCCCCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
YTEARHDACLNAVKGYCWITDTDTVLGRLQEMQP
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCC
>Mature Secondary Structure
MFSLPHRSPRDLPFATDHTALLLVDMQRAWLEPQFDPHLEGPDAAYFLNRTRSQVVPNQV
CCCCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCHHHH
RLLNAFRDARQNVLHTLIESLTADGRDRSLDHKLSDMHLPKGSPQAQVIAELTPAENEIV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHEEEEECCCCCCEE
LPKTSSGVFNSTNIDYVLRNLQTRHLIIAGIVTDQCVDMAVRDAADRGYLVTLVADACAT
EECCCCCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
YTEARHDACLNAVKGYCWITDTDTVLGRLQEMQP
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA