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The map label for this gene is zraS [H]

Identifier: 77458230

GI number: 77458230

Start: 2285878

End: 2287371

Strand: Direct

Name: zraS [H]

Synonym: Pfl01_2003

Alternate gene names: 77458230

Gene position: 2285878-2287371 (Clockwise)

Preceding gene: 77458229

Following gene: 77458231

Centisome position: 35.5

GC content: 60.31

Gene sequence:

>1494_bases
ATGCAACGACTGGAAGAACAGAAAACATCGGCACCGAACCTCGCGCTGGCGGCCATCAAGAGCTGGGGCGGGCAGTTCAA
TCTGCTGCGCTGGTTTTCGCTGGCAAGTTTTTTCATCATCGCGGCCGTGGCGCTGGGGCTGGGGTATATCTCCACGCGCT
TCGTGGTCACCGAAAGCGTGGAGCGCGATGCGCTGCTCACCGCGCAATTCGTGCAGGCCATCGGCGATGCAGAAATGCGC
CATGCCAACATCACGCCGCAACGGACCATGGGCGAGATGCTCGACCCGCGCCAGGACGAGAACTACCCCGACGTAGATCC
GCTGTCCCATGCAATGGCCCGTGCCGAGTTTCTCGATCATGTCGAACATTTGCCGGATGTTCTGCTGGCCACGGTGTACG
CCCTGGACCGCACGGTCATCTGGTCGACCAACCCCGAACTGATCAACGTCAAGTTTGAAGACGACGACGAGCTGGATGAG
TCGTTCGAGATGAAAGTCCCGGTGTCTTCCAGTTATCACAAGATCGACGATGAAAAACCCGAGCAGCGGCTTTCTCGCGA
ACCCAAATACCTGTTCATCGAAAACTACATTCCGATGTTCAACGCCGACAAAAGCAAAGTGGTCGCCATGGTCGAGATCT
ACAAGGAACCGGCGGATCTGGTCGACCGTATCGACCGAGGGTTCGCCTCGATCTGGGCTGCGACGATCCTCGGCGGCGCG
GCGATTTACCTCGGCTTGTTCTGGATCGTGCGGCGCGCCTCGACGCTGCTGCAGAGCCAGCAGCAGCAACTGATCAGCAA
TGAAACCTTTGTCGCTCTGGGCGAGATGTCCTCGGCGGTGGCCCACAGCCTGCGCAACCCGCTGGCGACCATTCGTTCCA
GCGCCGAACTGGCCCAGGAAATCGCCAGTCCGGGCGCACAGCGCAACATCGGCGACATCATCAGCCAGGTCGACCGCATG
TCGCGCTGGGTTCGCGAACTGCTGGTGTCGTTGCGCCCGATGAGTGACGACGGCGAGGCGGTGGATCTGCTGGCGGCGGT
CGAGGACACCCTGGGGGCGTTTGATGCGCTGATCCGGCGCAACGGCGTCGAAGTGCGATTCACGCCACAAGATTGCCCGC
CGGTGGTCAGTCAACAGGTGCTGCTCACGCAAATTCTCAATAGCCTGTTTGCCAATGCCCTGGAAGCGATGCCCAGAGGC
GGCGTACTCAGTGTCGAGATTGAAACGGCGCAGACCGGTCAGGTGCGCATGACCCTGAGCGACACTGGCAAAGGCATGAG
CGCGCAGCAGCAGCAACTGGTGTTCAAACCGTTTTTCACCACCAAGCAGGGCGGCCTCGGGGTCGGCCTGGCGCTGGTCA
AGCGAATCATGGAGCGGTTTCAGGGTTCGGTCATGCTGACCAGCCGCGAGCAGGAAGGAACCCGCGTCAGTCTCAACTTT
AGAGTGGCATCGGGAGGGGAATATGGAACACAGCATTCTGCTGGTCGAGGATGA

Upstream 100 bases:

>100_bases
AATAGCATCATTGAACCGTGTGTCACGGTCATTGCCACCTACACTCATTCGAGAGAGGGCGGCTGTAACCGGCTCGATAC
CAGAACGCCAGGGCATCCCC

Downstream 100 bases:

>100_bases
CGAACTGCTGGCCGAGAATATTCAGACCTACCTGGAGCGCAAAGACTTCGAGGTGACGGTCTGCCACTCGGCCGAGGACG
CGCTGGGGCAACTGGAAAGC

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MQRLEEQKTSAPNLALAAIKSWGGQFNLLRWFSLASFFIIAAVALGLGYISTRFVVTESVERDALLTAQFVQAIGDAEMR
HANITPQRTMGEMLDPRQDENYPDVDPLSHAMARAEFLDHVEHLPDVLLATVYALDRTVIWSTNPELINVKFEDDDELDE
SFEMKVPVSSSYHKIDDEKPEQRLSREPKYLFIENYIPMFNADKSKVVAMVEIYKEPADLVDRIDRGFASIWAATILGGA
AIYLGLFWIVRRASTLLQSQQQQLISNETFVALGEMSSAVAHSLRNPLATIRSSAELAQEIASPGAQRNIGDIISQVDRM
SRWVRELLVSLRPMSDDGEAVDLLAAVEDTLGAFDALIRRNGVEVRFTPQDCPPVVSQQVLLTQILNSLFANALEAMPRG
GVLSVEIETAQTGQVRMTLSDTGKGMSAQQQQLVFKPFFTTKQGGLGVGLALVKRIMERFQGSVMLTSREQEGTRVSLNF
RVASGGEYGTQHSAGRG

Sequences:

>Translated_497_residues
MQRLEEQKTSAPNLALAAIKSWGGQFNLLRWFSLASFFIIAAVALGLGYISTRFVVTESVERDALLTAQFVQAIGDAEMR
HANITPQRTMGEMLDPRQDENYPDVDPLSHAMARAEFLDHVEHLPDVLLATVYALDRTVIWSTNPELINVKFEDDDELDE
SFEMKVPVSSSYHKIDDEKPEQRLSREPKYLFIENYIPMFNADKSKVVAMVEIYKEPADLVDRIDRGFASIWAATILGGA
AIYLGLFWIVRRASTLLQSQQQQLISNETFVALGEMSSAVAHSLRNPLATIRSSAELAQEIASPGAQRNIGDIISQVDRM
SRWVRELLVSLRPMSDDGEAVDLLAAVEDTLGAFDALIRRNGVEVRFTPQDCPPVVSQQVLLTQILNSLFANALEAMPRG
GVLSVEIETAQTGQVRMTLSDTGKGMSAQQQQLVFKPFFTTKQGGLGVGLALVKRIMERFQGSVMLTSREQEGTRVSLNF
RVASGGEYGTQHSAGRG
>Mature_497_residues
MQRLEEQKTSAPNLALAAIKSWGGQFNLLRWFSLASFFIIAAVALGLGYISTRFVVTESVERDALLTAQFVQAIGDAEMR
HANITPQRTMGEMLDPRQDENYPDVDPLSHAMARAEFLDHVEHLPDVLLATVYALDRTVIWSTNPELINVKFEDDDELDE
SFEMKVPVSSSYHKIDDEKPEQRLSREPKYLFIENYIPMFNADKSKVVAMVEIYKEPADLVDRIDRGFASIWAATILGGA
AIYLGLFWIVRRASTLLQSQQQQLISNETFVALGEMSSAVAHSLRNPLATIRSSAELAQEIASPGAQRNIGDIISQVDRM
SRWVRELLVSLRPMSDDGEAVDLLAAVEDTLGAFDALIRRNGVEVRFTPQDCPPVVSQQVLLTQILNSLFANALEAMPRG
GVLSVEIETAQTGQVRMTLSDTGKGMSAQQQQLVFKPFFTTKQGGLGVGLALVKRIMERFQGSVMLTSREQEGTRVSLNF
RVASGGEYGTQHSAGRG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=250, Percent_Identity=34.4, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=30.3571428571429, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=29.2035398230088, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=325, Percent_Identity=25.5384615384615, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786783, Length=222, Percent_Identity=25.2252252252252, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790300, Length=218, Percent_Identity=25.2293577981651, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55072; Mature: 55072

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLEEQKTSAPNLALAAIKSWGGQFNLLRWFSLASFFIIAAVALGLGYISTRFVVTESV
CCCHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
ERDALLTAQFVQAIGDAEMRHANITPQRTMGEMLDPRQDENYPDVDPLSHAMARAEFLDH
CHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
VEHLPDVLLATVYALDRTVIWSTNPELINVKFEDDDELDESFEMKVPVSSSYHKIDDEKP
HHHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCH
EQRLSREPKYLFIENYIPMFNADKSKVVAMVEIYKEPADLVDRIDRGFASIWAATILGGA
HHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AIYLGLFWIVRRASTLLQSQQQQLISNETFVALGEMSSAVAHSLRNPLATIRSSAELAQE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IASPGAQRNIGDIISQVDRMSRWVRELLVSLRPMSDDGEAVDLLAAVEDTLGAFDALIRR
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NGVEVRFTPQDCPPVVSQQVLLTQILNSLFANALEAMPRGGVLSVEIETAQTGQVRMTLS
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEC
DTGKGMSAQQQQLVFKPFFTTKQGGLGVGLALVKRIMERFQGSVMLTSREQEGTRVSLNF
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEE
RVASGGEYGTQHSAGRG
EEECCCCCCCCCCCCCC
>Mature Secondary Structure
MQRLEEQKTSAPNLALAAIKSWGGQFNLLRWFSLASFFIIAAVALGLGYISTRFVVTESV
CCCHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
ERDALLTAQFVQAIGDAEMRHANITPQRTMGEMLDPRQDENYPDVDPLSHAMARAEFLDH
CHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
VEHLPDVLLATVYALDRTVIWSTNPELINVKFEDDDELDESFEMKVPVSSSYHKIDDEKP
HHHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCH
EQRLSREPKYLFIENYIPMFNADKSKVVAMVEIYKEPADLVDRIDRGFASIWAATILGGA
HHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AIYLGLFWIVRRASTLLQSQQQQLISNETFVALGEMSSAVAHSLRNPLATIRSSAELAQE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IASPGAQRNIGDIISQVDRMSRWVRELLVSLRPMSDDGEAVDLLAAVEDTLGAFDALIRR
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NGVEVRFTPQDCPPVVSQQVLLTQILNSLFANALEAMPRGGVLSVEIETAQTGQVRMTLS
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEC
DTGKGMSAQQQQLVFKPFFTTKQGGLGVGLALVKRIMERFQGSVMLTSREQEGTRVSLNF
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEE
RVASGGEYGTQHSAGRG
EEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]