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The map label for this gene is atoC [H]

Identifier: 77458231

GI number: 77458231

Start: 2287340

End: 2288776

Strand: Direct

Name: atoC [H]

Synonym: Pfl01_2004

Alternate gene names: 77458231

Gene position: 2287340-2288776 (Clockwise)

Preceding gene: 77458230

Following gene: 77458233

Centisome position: 35.53

GC content: 61.8

Gene sequence:

>1437_bases
ATGGAACACAGCATTCTGCTGGTCGAGGATGACGAACTGCTGGCCGAGAATATTCAGACCTACCTGGAGCGCAAAGACTT
CGAGGTGACGGTCTGCCACTCGGCCGAGGACGCGCTGGGGCAACTGGAAAGCTTCATGCCGGACATCGTCCTGACCGACA
ACTCGCTGCCGGGCATGAGCGGTCACGATCTGATCCAGAAGCTGCGCATCAGCGCGCCGGACCTGAAAGTGATCATGATG
ACCGGTTACGGCAACGTCGAAGACGCGGTGGTCGCGATGAAGGAGGGCGCCTTCCATTACGTCACCAAACCGGTCGCGCT
GCCGGAACTCAAACTGCTGCTGGACAAGGCCCTGGCCACCGAACGGATGGAACGCACGCTGTCGTTCTATCAGGAGCGCG
AGGCGCAGAAATCCGGAGTGCAGGCGCTGATCGGCGAATCGGCGCCTATGCTGCACCTGAAGAACACCATCGGTCAGTTG
CTCGACGCCGAGCGGCGCATGGCCAACGGCGATCTGCCGCCGGTTCTGGTGGAGGGCGAAACCGGTACCGGCAAGGAACT
GGTCGCCCGCGCGCTGCATTTCGACGGCGCGCGCAGCAAAGGCCCGTTCATTGAATTCAACTGCGCGTCGATTCCGGCCA
ACCTGGTGGAGTCAGAGCTGTTCGGTCACGAGAAAGGCGCGTTCACCGATGCCAAGGACCGCCGGGTCGGGCTGGTGGAA
GCCGCTGATGGCGGCACGCTGTTTCTCGATGAAATCGGTGAAATGGATCTGGTGCTCCAGGCTAAGCTGCTGAAATTGCT
GGAGGATCGCACCATTCGCCGGGTCGGCTCGGTGAAGGAGCGCAAGGTCAACCTGCGGGTGATCAGCGCCACCAATTGCA
ACCTTGAGCAGATGGTCCAGCAGGGCAAGTTCCGCCGCGATCTGTTTTTCCGGCTGCGGATCATCTCGATCAAGGTCCCG
CGCTTGTACGCCCGTGGCGAAGACATCCTGCTGCTGGCCCGGCACTTCCTCGCCAGCCACGGCAAGCGATATGGCAAACC
GAACCTGCACTTCAGCGATCAGGCCGAAGAGCTGCTGCTCAGTTACACCTGGCCGGGCAACGTGCGCGAACTGCGCAACA
TGCTCGAACAGACCGTGCTGCTGGCGCCAAACGACACCATCGCCGCTCATCAACTGAACGTGTGCATGAGCCTGTGCGAT
GAGCTGCCACCGCAACATCAGCACGAGGTGCCGCACACGCACTTCGAACCGCGCCCGGCGAGCAACACCGAATCGATGAA
CCTGCCGGAAGTCGAACGCGACATGGTGCGCAAGATGCTCGACAAGACCGACTGGAACGTCACCAAATCGGCGCGCCTGC
TGGGCCTGAGCCGCGACATGCTGCGCTACCGCATCGAAAAGCTCGGCCTGGCGCGGCCGGACAAGCGTCAGTGGTAA

Upstream 100 bases:

>100_bases
CGAATCATGGAGCGGTTTCAGGGTTCGGTCATGCTGACCAGCCGCGAGCAGGAAGGAACCCGCGTCAGTCTCAACTTTAG
AGTGGCATCGGGAGGGGAAT

Downstream 100 bases:

>100_bases
ACGGCGGTGCTGCGTGGATTCGGCGGGCTCAGGCATTCGAGCACGCACGTCGGGTCCAGCACCTCGTCGTTGACGTAAAT
GCCGCGCTCGGCATCGTAGG

Product: two component, sigma-54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MEHSILLVEDDELLAENIQTYLERKDFEVTVCHSAEDALGQLESFMPDIVLTDNSLPGMSGHDLIQKLRISAPDLKVIMM
TGYGNVEDAVVAMKEGAFHYVTKPVALPELKLLLDKALATERMERTLSFYQEREAQKSGVQALIGESAPMLHLKNTIGQL
LDAERRMANGDLPPVLVEGETGTGKELVARALHFDGARSKGPFIEFNCASIPANLVESELFGHEKGAFTDAKDRRVGLVE
AADGGTLFLDEIGEMDLVLQAKLLKLLEDRTIRRVGSVKERKVNLRVISATNCNLEQMVQQGKFRRDLFFRLRIISIKVP
RLYARGEDILLLARHFLASHGKRYGKPNLHFSDQAEELLLSYTWPGNVRELRNMLEQTVLLAPNDTIAAHQLNVCMSLCD
ELPPQHQHEVPHTHFEPRPASNTESMNLPEVERDMVRKMLDKTDWNVTKSARLLGLSRDMLRYRIEKLGLARPDKRQW

Sequences:

>Translated_478_residues
MEHSILLVEDDELLAENIQTYLERKDFEVTVCHSAEDALGQLESFMPDIVLTDNSLPGMSGHDLIQKLRISAPDLKVIMM
TGYGNVEDAVVAMKEGAFHYVTKPVALPELKLLLDKALATERMERTLSFYQEREAQKSGVQALIGESAPMLHLKNTIGQL
LDAERRMANGDLPPVLVEGETGTGKELVARALHFDGARSKGPFIEFNCASIPANLVESELFGHEKGAFTDAKDRRVGLVE
AADGGTLFLDEIGEMDLVLQAKLLKLLEDRTIRRVGSVKERKVNLRVISATNCNLEQMVQQGKFRRDLFFRLRIISIKVP
RLYARGEDILLLARHFLASHGKRYGKPNLHFSDQAEELLLSYTWPGNVRELRNMLEQTVLLAPNDTIAAHQLNVCMSLCD
ELPPQHQHEVPHTHFEPRPASNTESMNLPEVERDMVRKMLDKTDWNVTKSARLLGLSRDMLRYRIEKLGLARPDKRQW
>Mature_478_residues
MEHSILLVEDDELLAENIQTYLERKDFEVTVCHSAEDALGQLESFMPDIVLTDNSLPGMSGHDLIQKLRISAPDLKVIMM
TGYGNVEDAVVAMKEGAFHYVTKPVALPELKLLLDKALATERMERTLSFYQEREAQKSGVQALIGESAPMLHLKNTIGQL
LDAERRMANGDLPPVLVEGETGTGKELVARALHFDGARSKGPFIEFNCASIPANLVESELFGHEKGAFTDAKDRRVGLVE
AADGGTLFLDEIGEMDLVLQAKLLKLLEDRTIRRVGSVKERKVNLRVISATNCNLEQMVQQGKFRRDLFFRLRIISIKVP
RLYARGEDILLLARHFLASHGKRYGKPNLHFSDQAEELLLSYTWPGNVRELRNMLEQTVLLAPNDTIAAHQLNVCMSLCD
ELPPQHQHEVPHTHFEPRPASNTESMNLPEVERDMVRKMLDKTDWNVTKSARLLGLSRDMLRYRIEKLGLARPDKRQW

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=474, Percent_Identity=37.9746835443038, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1790437, Length=464, Percent_Identity=37.9310344827586, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1790299, Length=488, Percent_Identity=38.9344262295082, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788905, Length=454, Percent_Identity=36.784140969163, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI87082117, Length=304, Percent_Identity=42.7631578947368, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789233, Length=354, Percent_Identity=39.2655367231638, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1786524, Length=342, Percent_Identity=40.0584795321637, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=36.1963190184049, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082152, Length=335, Percent_Identity=40.2985074626866, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789087, Length=426, Percent_Identity=34.037558685446, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1787583, Length=202, Percent_Identity=44.5544554455446, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789828, Length=268, Percent_Identity=34.7014925373134, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=29.2929292929293, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1786599, Length=112, Percent_Identity=35.7142857142857, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54016; Mature: 54016

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHSILLVEDDELLAENIQTYLERKDFEVTVCHSAEDALGQLESFMPDIVLTDNSLPGMS
CCCCEEEEECHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCEEEECCCCCCCC
GHDLIQKLRISAPDLKVIMMTGYGNVEDAVVAMKEGAFHYVTKPVALPELKLLLDKALAT
HHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
ERMERTLSFYQEREAQKSGVQALIGESAPMLHLKNTIGQLLDAERRMANGDLPPVLVEGE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
TGTGKELVARALHFDGARSKGPFIEFNCASIPANLVESELFGHEKGAFTDAKDRRVGLVE
CCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEE
AADGGTLFLDEIGEMDLVLQAKLLKLLEDRTIRRVGSVKERKVNLRVISATNCNLEQMVQ
ECCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEEEEECCCCHHHHHH
QGKFRRDLFFRLRIISIKVPRLYARGEDILLLARHFLASHGKRYGKPNLHFSDQAEELLL
CCCHHHHHHHEEEEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEE
SYTWPGNVRELRNMLEQTVLLAPNDTIAAHQLNVCMSLCDELPPQHQHEVPHTHFEPRPA
EECCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
SNTESMNLPEVERDMVRKMLDKTDWNVTKSARLLGLSRDMLRYRIEKLGLARPDKRQW
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEHSILLVEDDELLAENIQTYLERKDFEVTVCHSAEDALGQLESFMPDIVLTDNSLPGMS
CCCCEEEEECHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCEEEECCCCCCCC
GHDLIQKLRISAPDLKVIMMTGYGNVEDAVVAMKEGAFHYVTKPVALPELKLLLDKALAT
HHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
ERMERTLSFYQEREAQKSGVQALIGESAPMLHLKNTIGQLLDAERRMANGDLPPVLVEGE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
TGTGKELVARALHFDGARSKGPFIEFNCASIPANLVESELFGHEKGAFTDAKDRRVGLVE
CCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEE
AADGGTLFLDEIGEMDLVLQAKLLKLLEDRTIRRVGSVKERKVNLRVISATNCNLEQMVQ
ECCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEEEEECCCCHHHHHH
QGKFRRDLFFRLRIISIKVPRLYARGEDILLLARHFLASHGKRYGKPNLHFSDQAEELLL
CCCHHHHHHHEEEEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEE
SYTWPGNVRELRNMLEQTVLLAPNDTIAAHQLNVCMSLCDELPPQHQHEVPHTHFEPRPA
EECCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
SNTESMNLPEVERDMVRKMLDKTDWNVTKSARLLGLSRDMLRYRIEKLGLARPDKRQW
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]