Click here to switch to the map view.

The map label for this gene is thrS [H]

Identifier: 77458158

GI number: 77458158

Start: 2200461

End: 2202383

Strand: Direct

Name: thrS [H]

Synonym: Pfl01_1931

Alternate gene names: 77458158

Gene position: 2200461-2202383 (Clockwise)

Preceding gene: 77458155

Following gene: 77458159

Centisome position: 34.18

GC content: 57.46

Gene sequence:

>1923_bases
ATGCCAACTATTACTCTTCCCGACGGCAGTCAACGTTCATTCGATCACCCGGTTTCCGTAGCCGAGGTCGCCGCCTCCAT
CGGTGCAGGTCTGGCCAAGGCCACCGTAGCCGGCAAGGTCAATGGCAAGCTGGTCGACGCCTGCGACATCATCGACAGCG
ATGCGACGCTGCAAATCATCACGCCAAAGGATGAAGAGGGGCTGGAGATCATTCGCCACTCTTGCGCCCACCTGGTCGGT
CATGCGGTCAAGCAGCTGTACCCGACTGCAAAAATGGTCATCGGGCCGGTCATCGACGAAGGTTTCTATTACGACATTGC
CTTCGAGCGTCCTTTCACTCCGGACGACATGGCGGCCATCGAACAGCGCATGCACCAGCTGATCGATACAGAATATGACG
TCATCAAGAAAGTCACTCCGCGTGCCGAAGTGATCGAAGTGTTCAAGGCCCGTGGCGAAGACTACAAGCTGCGCCTGGTC
GAAGACATGCCGAACGAGCAGGCCATGGGCCTGTACTATCACGAAGAATACGTCGACATGTGCCGCGGTCCGCACGTGCC
GAACACTCGCTTCCTGAAATCCTTCAAGCTGACCAAGCTGTCCGGCGCCTACTGGCGCGGTGATGCCAAGAACGAGCAAT
TGCAGCGCGTTTATGGCACCGCGTGGGCTGACAAGAAGCAACTGGCTGCTTACATCCAGCGTATCGAAGAAGCTGAGAAG
CGCGATCACCGCAAGATAGGCAAGCGCCTGGGTCTGTTTCACACCCAGGAGGAAGCGCCGGGCATGGTGTTCTGGCACCC
GAATGGCTGGACGCTGTACCAGGTACTCGAGCAGTACATGCGCAAGGTGCAGCGCGATAACGGCTATCTCGAGATCAAGA
CGCCGCAAGTCGTTGACCGCAGCCTGTGGGAGAAATCCGGGCATTGGGCCAACTACGCCGACAACATGTTCACCACTGAG
TCGGAAAGCCGCGACTACGCCATCAAGCCAATGAACTGCCCATGCCACGTGCAGGTGTTCAACCAGGGCTTGAAGAGCTA
CCGCGAGCTGCCGATGCGTCTTGCCGAGTTCGGCGCCTGCCACCGTAACGAGCCATCGGGTGCGCTGCACGGCATCATGC
GTGTGCGTGCGTTCACTCAGGACGACGCCCACATCTTCTGCACCGAAGAGCAGATGCAGGCTGAATCCGCTGCGTTCATC
AAGCTGACCATGGATGTTTACCGCGACTTCGGCTTTACCGACGTCGAGATGAAGCTGTCCACTCGTCCGGAAAAACGCGT
CGGTTCCGACGAGCTGTGGGATCGCGCCGAAGCAGCTCTGGCTGCAGCCCTTGATAGCGCGGGTCTGCCTTACGATCTGC
AGCCGGGTGAGGGGGCGTTCTACGGTCCGAAAATCGAATTCTCGCTGAAAGATTGCCTCGGCCGCGTGTGGCAGTGTGGT
ACCTTGCAGCTCGATTTCAACCTGCCTGTGCGTCTGGGAGCCGAATACGTTTCCGAAGACAACAGCCGCAAGCATCCGGT
GATGTTGCACCGTGCGATCCTCGGTTCGTTCGAGCGTTTCGTCGGAATTCTGATCGAGCACTACGAGGGTGCGTTCCCTG
CGTGGCTGGCGCCAACCCAGGCAGTGATCATGAATATCACTGATAAACAGGCAGATTTCGTCGTTCAGGTCGAAAAAACT
CTCAACGAAAGCGGGTTTCGTGCCAAGTCCGACTTGAGAAATGAAAAGATCGGCTTTAAAATCCGCGAGCATACTTTGCT
CAAGGTTCCATATCTCTTGGTTATTGGGGATAAGGAAGTCGAGATGCAGACTGTCGCTGTGCGTACTCGTGAAGGTGCTG
ACCTGGGCTCGATGCCCGTCGCCCAGTTCGCTGAGTTTCTCGCGCAAGCGGTTTCCCGGCGTGGTCGCCCAGATTCGGAG
TAA

Upstream 100 bases:

>100_bases
GGCAGGCGTTCGTCATTGAATGGCCTGGATCGGATCTTGTACTGGCTCATCCCAACCCACGTGACCTTTGGTAGGGGTCA
CCACTAGGAGAGGAGGCGCC

Downstream 100 bases:

>100_bases
TTATTATTAAGCGTGAAATGAGACAAGATAAACGAGCTGCACCGAAAGCCCCGATCAACGAGAATATCTCGGCACGCGAG
GTTCGGTTAATTGGCGCTGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MPTITLPDGSQRSFDHPVSVAEVAASIGAGLAKATVAGKVNGKLVDACDIIDSDATLQIITPKDEEGLEIIRHSCAHLVG
HAVKQLYPTAKMVIGPVIDEGFYYDIAFERPFTPDDMAAIEQRMHQLIDTEYDVIKKVTPRAEVIEVFKARGEDYKLRLV
EDMPNEQAMGLYYHEEYVDMCRGPHVPNTRFLKSFKLTKLSGAYWRGDAKNEQLQRVYGTAWADKKQLAAYIQRIEEAEK
RDHRKIGKRLGLFHTQEEAPGMVFWHPNGWTLYQVLEQYMRKVQRDNGYLEIKTPQVVDRSLWEKSGHWANYADNMFTTE
SESRDYAIKPMNCPCHVQVFNQGLKSYRELPMRLAEFGACHRNEPSGALHGIMRVRAFTQDDAHIFCTEEQMQAESAAFI
KLTMDVYRDFGFTDVEMKLSTRPEKRVGSDELWDRAEAALAAALDSAGLPYDLQPGEGAFYGPKIEFSLKDCLGRVWQCG
TLQLDFNLPVRLGAEYVSEDNSRKHPVMLHRAILGSFERFVGILIEHYEGAFPAWLAPTQAVIMNITDKQADFVVQVEKT
LNESGFRAKSDLRNEKIGFKIREHTLLKVPYLLVIGDKEVEMQTVAVRTREGADLGSMPVAQFAEFLAQAVSRRGRPDSE

Sequences:

>Translated_640_residues
MPTITLPDGSQRSFDHPVSVAEVAASIGAGLAKATVAGKVNGKLVDACDIIDSDATLQIITPKDEEGLEIIRHSCAHLVG
HAVKQLYPTAKMVIGPVIDEGFYYDIAFERPFTPDDMAAIEQRMHQLIDTEYDVIKKVTPRAEVIEVFKARGEDYKLRLV
EDMPNEQAMGLYYHEEYVDMCRGPHVPNTRFLKSFKLTKLSGAYWRGDAKNEQLQRVYGTAWADKKQLAAYIQRIEEAEK
RDHRKIGKRLGLFHTQEEAPGMVFWHPNGWTLYQVLEQYMRKVQRDNGYLEIKTPQVVDRSLWEKSGHWANYADNMFTTE
SESRDYAIKPMNCPCHVQVFNQGLKSYRELPMRLAEFGACHRNEPSGALHGIMRVRAFTQDDAHIFCTEEQMQAESAAFI
KLTMDVYRDFGFTDVEMKLSTRPEKRVGSDELWDRAEAALAAALDSAGLPYDLQPGEGAFYGPKIEFSLKDCLGRVWQCG
TLQLDFNLPVRLGAEYVSEDNSRKHPVMLHRAILGSFERFVGILIEHYEGAFPAWLAPTQAVIMNITDKQADFVVQVEKT
LNESGFRAKSDLRNEKIGFKIREHTLLKVPYLLVIGDKEVEMQTVAVRTREGADLGSMPVAQFAEFLAQAVSRRGRPDSE
>Mature_639_residues
PTITLPDGSQRSFDHPVSVAEVAASIGAGLAKATVAGKVNGKLVDACDIIDSDATLQIITPKDEEGLEIIRHSCAHLVGH
AVKQLYPTAKMVIGPVIDEGFYYDIAFERPFTPDDMAAIEQRMHQLIDTEYDVIKKVTPRAEVIEVFKARGEDYKLRLVE
DMPNEQAMGLYYHEEYVDMCRGPHVPNTRFLKSFKLTKLSGAYWRGDAKNEQLQRVYGTAWADKKQLAAYIQRIEEAEKR
DHRKIGKRLGLFHTQEEAPGMVFWHPNGWTLYQVLEQYMRKVQRDNGYLEIKTPQVVDRSLWEKSGHWANYADNMFTTES
ESRDYAIKPMNCPCHVQVFNQGLKSYRELPMRLAEFGACHRNEPSGALHGIMRVRAFTQDDAHIFCTEEQMQAESAAFIK
LTMDVYRDFGFTDVEMKLSTRPEKRVGSDELWDRAEAALAAALDSAGLPYDLQPGEGAFYGPKIEFSLKDCLGRVWQCGT
LQLDFNLPVRLGAEYVSEDNSRKHPVMLHRAILGSFERFVGILIEHYEGAFPAWLAPTQAVIMNITDKQADFVVQVEKTL
NESGFRAKSDLRNEKIGFKIREHTLLKVPYLLVIGDKEVEMQTVAVRTREGADLGSMPVAQFAEFLAQAVSRRGRPDSE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=641, Percent_Identity=38.8455538221529, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI38570111, Length=603, Percent_Identity=39.9668325041459, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI20070344, Length=652, Percent_Identity=35.4294478527607, Blast_Score=394, Evalue=1e-109,
Organism=Escherichia coli, GI1788013, Length=635, Percent_Identity=65.3543307086614, Blast_Score=901, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=158, Percent_Identity=25.9493670886076, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71984192, Length=637, Percent_Identity=39.5604395604396, Blast_Score=477, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71984184, Length=637, Percent_Identity=39.5604395604396, Blast_Score=477, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322112, Length=628, Percent_Identity=39.3312101910828, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=40.3022670025189, Blast_Score=319, Evalue=9e-88,
Organism=Drosophila melanogaster, GI24583842, Length=623, Percent_Identity=40.1284109149278, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI28574141, Length=623, Percent_Identity=40.1284109149278, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24583839, Length=623, Percent_Identity=40.1284109149278, Blast_Score=498, Evalue=1e-141,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 72543; Mature: 72412

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTITLPDGSQRSFDHPVSVAEVAASIGAGLAKATVAGKVNGKLVDACDIIDSDATLQII
CCEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHEECCCCCCEEEHHHCCCCCCEEEEE
TPKDEEGLEIIRHSCAHLVGHAVKQLYPTAKMVIGPVIDEGFYYDIAFERPFTPDDMAAI
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
EQRMHQLIDTEYDVIKKVTPRAEVIEVFKARGEDYKLRLVEDMPNEQAMGLYYHEEYVDM
HHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEHHHHHHH
CRGPHVPNTRFLKSFKLTKLSGAYWRGDAKNEQLQRVYGTAWADKKQLAAYIQRIEEAEK
HCCCCCCCHHHHHHHHHEECCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RDHRKIGKRLGLFHTQEEAPGMVFWHPNGWTLYQVLEQYMRKVQRDNGYLEIKTPQVVDR
HHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHH
SLWEKSGHWANYADNMFTTESESRDYAIKPMNCPCHVQVFNQGLKSYRELPMRLAEFGAC
HHHHCCCCCCCCCCCCEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
HRNEPSGALHGIMRVRAFTQDDAHIFCTEEQMQAESAAFIKLTMDVYRDFGFTDVEMKLS
CCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHEEEEEHHHHHCCCCCEEEEEEC
TRPEKRVGSDELWDRAEAALAAALDSAGLPYDLQPGEGAFYGPKIEFSLKDCLGRVWQCG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCEEEEEHHHHHHHHHCCC
TLQLDFNLPVRLGAEYVSEDNSRKHPVMLHRAILGSFERFVGILIEHYEGAFPAWLAPTQ
EEEEECCCCHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCE
AVIMNITDKQADFVVQVEKTLNESGFRAKSDLRNEKIGFKIREHTLLKVPYLLVIGDKEV
EEEEECCCCCCCEEEEEHHHHCCCCCCCHHHHCCCCCCEEEECCEEEECCEEEEECCCCC
EMQTVAVRTREGADLGSMPVAQFAEFLAQAVSRRGRPDSE
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PTITLPDGSQRSFDHPVSVAEVAASIGAGLAKATVAGKVNGKLVDACDIIDSDATLQII
CEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHEECCCCCCEEEHHHCCCCCCEEEEE
TPKDEEGLEIIRHSCAHLVGHAVKQLYPTAKMVIGPVIDEGFYYDIAFERPFTPDDMAAI
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
EQRMHQLIDTEYDVIKKVTPRAEVIEVFKARGEDYKLRLVEDMPNEQAMGLYYHEEYVDM
HHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEHHHHHHH
CRGPHVPNTRFLKSFKLTKLSGAYWRGDAKNEQLQRVYGTAWADKKQLAAYIQRIEEAEK
HCCCCCCCHHHHHHHHHEECCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RDHRKIGKRLGLFHTQEEAPGMVFWHPNGWTLYQVLEQYMRKVQRDNGYLEIKTPQVVDR
HHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHH
SLWEKSGHWANYADNMFTTESESRDYAIKPMNCPCHVQVFNQGLKSYRELPMRLAEFGAC
HHHHCCCCCCCCCCCCEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
HRNEPSGALHGIMRVRAFTQDDAHIFCTEEQMQAESAAFIKLTMDVYRDFGFTDVEMKLS
CCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHEEEEEHHHHHCCCCCEEEEEEC
TRPEKRVGSDELWDRAEAALAAALDSAGLPYDLQPGEGAFYGPKIEFSLKDCLGRVWQCG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCEEEEEHHHHHHHHHCCC
TLQLDFNLPVRLGAEYVSEDNSRKHPVMLHRAILGSFERFVGILIEHYEGAFPAWLAPTQ
EEEEECCCCHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCE
AVIMNITDKQADFVVQVEKTLNESGFRAKSDLRNEKIGFKIREHTLLKVPYLLVIGDKEV
EEEEECCCCCCCEEEEEHHHHCCCCCCCHHHHCCCCCCEEEECCEEEECCEEEEECCCCC
EMQTVAVRTREGADLGSMPVAQFAEFLAQAVSRRGRPDSE
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA