Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 77458116

GI number: 77458116

Start: 2158122

End: 2159540

Strand: Direct

Name: leuC [H]

Synonym: Pfl01_1889

Alternate gene names: 77458116

Gene position: 2158122-2159540 (Clockwise)

Preceding gene: 77458112

Following gene: 77458117

Centisome position: 33.52

GC content: 62.51

Gene sequence:

>1419_bases
ATGGCCGGCAAAACGCTCTACGACAAGCTCTGGGATTCGCATTTGGTCAAGCAGCGCGACGATGGCTCGGCGCTGATCTA
TATCGATCGTCATATCATTCACGAAGTGACCTCGCCGCAAGCCTTTGAAGGCCTGCGTCTGGCCGGACGCAAGCCGTGGC
GCATCGATGCCAACATCGCCACCCCGGACCACAACGTACCGACCACTCCGGAGCGCAAGGGCGGCATCGAAGCCATTGCC
GACCAGGTCTCGCGTTTGCAGGTTCAGACCCTCGATGACAACTGCGATGAATACGGCATCGTCGAGTTCAAGATGAACGA
CGTGCGCCAAGGCATCGTTCACGTGATCAGCCCGGAGCAGGGCGCGACCTTGCCGGGCATGACCGTGGTCTGCGGCGACT
CGCACACCTCGACCCACGGCGCGTTCGGCGCCTTGGCCCACGGGATCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACC
CAGTGCCTGGTCGCCAAGAAAATGAAGAACATGCTGGTGCGCGTCGAAGGTCAGTTGCCGTTCGGCGTGACCGCCAAGGA
CATCGTCCTCGCGGTGATCGGCAAGATCGGCACCGCTGGCGGTAACGGCCATGCGATCGAGTTCGCCGGCAGCGCGATCC
GCGATCTGTCCATCGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGTCTGGTGGCGGCG
GACCAGAAGACTGTGGATTACGTCAAGGGTCGTCCTTTCGCACCGAAAGGTGCCGAGTGGGATCTGGCGGTCGAGTCCTG
GAAAGACCTGGTGTCCGACGCCGATGCGCATTTCGACACCGTGGTCGAGCTCGACGCCACGCAGATCAAGCCGCAGGTCA
GCTGGGGCACTTCGCCGGAAATGGTCTTGGCCGTTGATCAGAACGTGCCGGACCCGGCTAAAGAGATGGATCTGGTCAAA
CGCGACTCGATCGTCCGCGCCTTGAAATACATGGGTTTGACCGCCAATCAGGCGATCACTGACATTCAGCTCGACCGCGT
GTTCATCGGCTCCTGCACCAACTCGCGCATCGAAGACTTGCGCGCTGCGGCGGTGATCGCCAAGGGTCGCAAGGTTGCGT
CGACCATCAAGCAGGCCATCGTCGTGCCGGGCTCGGGTCTGGTGAAGGCGCAGGCCGAGGCCGAAGGTCTGGACAAGATT
TTCCTCGAAGCCGGTTTCGAATGGCGCGAGCCGGGTTGCTCGATGTGCCTGGCGATGAACCCGGACCGTCTGGAGTCCGG
CGAGCATTGCGCCTCGACCTCCAACCGTAACTTCGAAGGCCGTCAGGGCGCCGGTGGCCGTACCCACCTCGTCAGCCCGG
CCATGGCCGCTGCGGCGGCGGTGAACGGTCGTTTCATCGACGTTCGTGAATTGATCTAA

Upstream 100 bases:

>100_bases
AATTTGAGTTATTTAATCTAACCCCATAGGATCGGCCTCACAAGCCAAAGGGTTATTGATAAGCCCAGGGCATAGAAACA
AGCTGATGAGGAACCGTCTG

Downstream 100 bases:

>100_bases
AGGAGCGCAGCATGAAAGCTTTTACCCAGCACACTGGTCTCGTCGCGCCTCTGGATCGTGCCAACGTCGACACCGACCAG
ATCATTCCGAAGCAGTTTCT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAGRKPWRIDANIATPDHNVPTTPERKGGIEAIA
DQVSRLQVQTLDDNCDEYGIVEFKMNDVRQGIVHVISPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QCLVAKKMKNMLVRVEGQLPFGVTAKDIVLAVIGKIGTAGGNGHAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGLVAA
DQKTVDYVKGRPFAPKGAEWDLAVESWKDLVSDADAHFDTVVELDATQIKPQVSWGTSPEMVLAVDQNVPDPAKEMDLVK
RDSIVRALKYMGLTANQAITDIQLDRVFIGSCTNSRIEDLRAAAVIAKGRKVASTIKQAIVVPGSGLVKAQAEAEGLDKI
FLEAGFEWREPGCSMCLAMNPDRLESGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRELI

Sequences:

>Translated_472_residues
MAGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAGRKPWRIDANIATPDHNVPTTPERKGGIEAIA
DQVSRLQVQTLDDNCDEYGIVEFKMNDVRQGIVHVISPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QCLVAKKMKNMLVRVEGQLPFGVTAKDIVLAVIGKIGTAGGNGHAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGLVAA
DQKTVDYVKGRPFAPKGAEWDLAVESWKDLVSDADAHFDTVVELDATQIKPQVSWGTSPEMVLAVDQNVPDPAKEMDLVK
RDSIVRALKYMGLTANQAITDIQLDRVFIGSCTNSRIEDLRAAAVIAKGRKVASTIKQAIVVPGSGLVKAQAEAEGLDKI
FLEAGFEWREPGCSMCLAMNPDRLESGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRELI
>Mature_471_residues
AGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAGRKPWRIDANIATPDHNVPTTPERKGGIEAIAD
QVSRLQVQTLDDNCDEYGIVEFKMNDVRQGIVHVISPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQ
CLVAKKMKNMLVRVEGQLPFGVTAKDIVLAVIGKIGTAGGNGHAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGLVAAD
QKTVDYVKGRPFAPKGAEWDLAVESWKDLVSDADAHFDTVVELDATQIKPQVSWGTSPEMVLAVDQNVPDPAKEMDLVKR
DSIVRALKYMGLTANQAITDIQLDRVFIGSCTNSRIEDLRAAAVIAKGRKVASTIKQAIVVPGSGLVKAQAEAEGLDKIF
LEAGFEWREPGCSMCLAMNPDRLESGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRELI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=364, Percent_Identity=28.021978021978, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI41352693, Length=382, Percent_Identity=26.4397905759162, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=63.8888888888889, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=27.3480662983425, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=23.249299719888, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367097, Length=372, Percent_Identity=24.1935483870968, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=407, Percent_Identity=28.2555282555283, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=407, Percent_Identity=28.2555282555283, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=27.9605263157895, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=400, Percent_Identity=27.25, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=60.6382978723404, Blast_Score=586, Evalue=1e-168,
Organism=Saccharomyces cerevisiae, GI6323335, Length=365, Percent_Identity=30.1369863013699, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=355, Percent_Identity=29.8591549295775, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=362, Percent_Identity=27.9005524861878, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281365315, Length=399, Percent_Identity=27.8195488721804, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17864292, Length=399, Percent_Identity=27.8195488721804, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161076999, Length=400, Percent_Identity=27.75, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571643, Length=396, Percent_Identity=26.2626262626263, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24645686, Length=377, Percent_Identity=27.8514588859416, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137564, Length=377, Percent_Identity=27.0557029177719, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50748; Mature: 50617

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAGRKPWRIDANIA
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCEEEECEEC
TPDHNVPTTPERKGGIEAIADQVSRLQVQTLDDNCDEYGIVEFKMNDVRQGIVHVISPEQ
CCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCEEEEEHHHHHCCCEEEECCCC
GATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVAKKMKNMLVRVEGQLP
CCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
FGVTAKDIVLAVIGKIGTAGGNGHAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGLVAA
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHEEECCCCEEEEEEECCCCCEEEEEEC
DQKTVDYVKGRPFAPKGAEWDLAVESWKDLVSDADAHFDTVVELDATQIKPQVSWGTSPE
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHHCCCEECCCCCCC
MVLAVDQNVPDPAKEMDLVKRDSIVRALKYMGLTANQAITDIQLDRVFIGSCTNSRIEDL
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECEEEEECCCCHHHHHH
RAAAVIAKGRKVASTIKQAIVVPGSGLVKAQAEAEGLDKIFLEAGFEWREPGCSMCLAMN
HHHHHHHCCHHHHHHHHHEEECCCCCCEEECHHHCCHHHHHHHCCCCCCCCCCCEEEEEC
PDRLESGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRELI
HHHCCCCCHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHCCCCEEEHHHCC
>Mature Secondary Structure 
AGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAGRKPWRIDANIA
CCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCEEEECEEC
TPDHNVPTTPERKGGIEAIADQVSRLQVQTLDDNCDEYGIVEFKMNDVRQGIVHVISPEQ
CCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCEEEEEHHHHHCCCEEEECCCC
GATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVAKKMKNMLVRVEGQLP
CCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
FGVTAKDIVLAVIGKIGTAGGNGHAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGLVAA
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHEEECCCCEEEEEEECCCCCEEEEEEC
DQKTVDYVKGRPFAPKGAEWDLAVESWKDLVSDADAHFDTVVELDATQIKPQVSWGTSPE
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHHCCCEECCCCCCC
MVLAVDQNVPDPAKEMDLVKRDSIVRALKYMGLTANQAITDIQLDRVFIGSCTNSRIEDL
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECEEEEECCCCHHHHHH
RAAAVIAKGRKVASTIKQAIVVPGSGLVKAQAEAEGLDKIFLEAGFEWREPGCSMCLAMN
HHHHHHHCCHHHHHHHHHEEECCCCCCEEECHHHCCHHHHHHHCCCCCCCCCCCEEEEEC
PDRLESGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFIDVRELI
HHHCCCCCHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHCCCCEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]