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The map label for this gene is mii [H]

Identifier: 77457994

GI number: 77457994

Start: 1963888

End: 1965099

Strand: Direct

Name: mii [H]

Synonym: Pfl01_1767

Alternate gene names: 77457994

Gene position: 1963888-1965099 (Clockwise)

Preceding gene: 77457993

Following gene: 77457995

Centisome position: 30.5

GC content: 61.06

Gene sequence:

>1212_bases
ATGTCTTCGTTAAGGAGCACCATGGCTCACGCACCGCAAATAAAAATCCCCGCCACCTACATGCGTGGCGGCACCAGCAA
AGGCGTGTTTTTCAGCCTCAAGGACCTGCCCGAAGCCGCGCAGATTCCCGGCCCGGCCCGCGATGCGCTGTTGCTGCGGG
TCATCGGCAGCCCCGATCCGTACGACAAGCAGATCGACGGCATGGGCGGCGCCACCTCGAGCACCAGCAAAACCGTAATC
CTGTCGAAAAGCATCAAGGCCGATCACGACGTCGATTACCTGTTCGGTCAGGTGTCGATCGACAAGCCGTTTGTGGACTG
GAGTGGCAACTGCGGCAACCTGTCGGCGGCGGTCGGCTCGTTTGCCATCAGCAACGGTCTGGTGGACGCCAGTCGCATTC
CGCACAACGGTGTGGCGGTGGTTCGCGTGTGGCAGGCCAACATCGGTAAAACGATCATTGCCCACGTGCCGATCACCAAC
GGCGAAGTCCAGGAAACCGGTGATTTCGAACTCGACGGCGTGACCTTCCCGGCTGCCGAAGTCCAGGTCGAATTCATGGA
CCCGGCGGCGGAAGAAGAGGGCGGCGGTGGCTCGATGTTCCCCACCGGCAACCTGATCGACGATCTGGAAGTGCCGGGCG
TCGGCACGTTCAAGGCGACCATGATCAACGCGGGTATTCCGACGATCTTCGTCAACGCCGAAGACATCGGTTACACCGGT
ACTGAATTGCAAAGCGCGATCAACAGCGATCCGAAAGCGTTACAGATGTTTGAGACCATTCGTGCCTACGGTGCTTTGCG
CATGGGGCTGATTTCCAGTCTGGATGAAGCGGCCAGGCGTCAGCACACGCCTAAGGTAGCGTTCGTCGCCAAGCCTGCGG
ATTACGTCGCGTCCAGTGGCAAAGCCATTGGCGCCGGGGATGTGGATTTGCTGGTGCGGGCGTTGTCGATGGGCAAGCTG
CACCACGCGATGATGGGGACGGCGGCGGTGGCGATCGGCACGGCGGCGGCCATCTCGGGCACGTTGGTGAATCTGGCGGC
GGGTGGTGTGGAGCGGAATGCGGTGCGGTTCGGGCATCCGTCCGGGACGTTGCGTGTTGGCGCAGAAGCTAGTCTGGAAA
ACGGTGAGTGGGTTGTGAAGAAAGCGATCATGAGCCGTAGTGCGCGCGTCCTGATGGAAGGTTACGTGCGCGTTCCTGGC
GACTCGTTCTAA

Upstream 100 bases:

>100_bases
CGAGGCTGGCGGCGTGTTGCAGCGCTTCGCTCAGGACTTCCTCGAAGAGTCGGCGGTTGCCGTTTAAATCAACTGATGTG
GGCATGGGACTTCAAGTCCC

Downstream 100 bases:

>100_bases
TGAACAACCGGTGAGAGCGAGCCCGTTCGCTCTCACCGCCATGCACATTTCAATAAGAAGAAAATTAGCCCTGAACCGAG
GTCCCATCAACGAACCACTT

Product: methylitaconate delta2-delta3-isomerase

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSSLRSTMAHAPQIKIPATYMRGGTSKGVFFSLKDLPEAAQIPGPARDALLLRVIGSPDPYDKQIDGMGGATSSTSKTVI
LSKSIKADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGSFAISNGLVDASRIPHNGVAVVRVWQANIGKTIIAHVPITN
GEVQETGDFELDGVTFPAAEVQVEFMDPAAEEEGGGGSMFPTGNLIDDLEVPGVGTFKATMINAGIPTIFVNAEDIGYTG
TELQSAINSDPKALQMFETIRAYGALRMGLISSLDEAARRQHTPKVAFVAKPADYVASSGKAIGAGDVDLLVRALSMGKL
HHAMMGTAAVAIGTAAAISGTLVNLAAGGVERNAVRFGHPSGTLRVGAEASLENGEWVVKKAIMSRSARVLMEGYVRVPG
DSF

Sequences:

>Translated_403_residues
MSSLRSTMAHAPQIKIPATYMRGGTSKGVFFSLKDLPEAAQIPGPARDALLLRVIGSPDPYDKQIDGMGGATSSTSKTVI
LSKSIKADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGSFAISNGLVDASRIPHNGVAVVRVWQANIGKTIIAHVPITN
GEVQETGDFELDGVTFPAAEVQVEFMDPAAEEEGGGGSMFPTGNLIDDLEVPGVGTFKATMINAGIPTIFVNAEDIGYTG
TELQSAINSDPKALQMFETIRAYGALRMGLISSLDEAARRQHTPKVAFVAKPADYVASSGKAIGAGDVDLLVRALSMGKL
HHAMMGTAAVAIGTAAAISGTLVNLAAGGVERNAVRFGHPSGTLRVGAEASLENGEWVVKKAIMSRSARVLMEGYVRVPG
DSF
>Mature_402_residues
SSLRSTMAHAPQIKIPATYMRGGTSKGVFFSLKDLPEAAQIPGPARDALLLRVIGSPDPYDKQIDGMGGATSSTSKTVIL
SKSIKADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGSFAISNGLVDASRIPHNGVAVVRVWQANIGKTIIAHVPITNG
EVQETGDFELDGVTFPAAEVQVEFMDPAAEEEGGGGSMFPTGNLIDDLEVPGVGTFKATMINAGIPTIFVNAEDIGYTGT
ELQSAINSDPKALQMFETIRAYGALRMGLISSLDEAARRQHTPKVAFVAKPADYVASSGKAIGAGDVDLLVRALSMGKLH
HAMMGTAAVAIGTAAAISGTLVNLAAGGVERNAVRFGHPSGTLRVGAEASLENGEWVVKKAIMSRSARVLMEGYVRVPGD
SF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=388, Percent_Identity=34.5360824742268, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 42117; Mature: 41986

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLRSTMAHAPQIKIPATYMRGGTSKGVFFSLKDLPEAAQIPGPARDALLLRVIGSPDP
CCHHHHHHHCCCCEECCHHHHCCCCCCCEEEEECCCCCHHCCCCCCCCEEEEEEECCCCC
YDKQIDGMGGATSSTSKTVILSKSIKADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGS
CCCHHCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHH
FAISNGLVDASRIPHNGVAVVRVWQANIGKTIIAHVPITNGEVQETGDFELDGVTFPAAE
HHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHE
VQVEFMDPAAEEEGGGGSMFPTGNLIDDLEVPGVGTFKATMINAGIPTIFVNAEDIGYTG
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECHHCCCCH
TELQSAINSDPKALQMFETIRAYGALRMGLISSLDEAARRQHTPKVAFVAKPADYVASSG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCC
KAIGAGDVDLLVRALSMGKLHHAMMGTAAVAIGTAAAISGTLVNLAAGGVERNAVRFGHP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCCCEEEECCC
SGTLRVGAEASLENGEWVVKKAIMSRSARVLMEGYVRVPGDSF
CCCEEECCCCCCCCCHHHHHHHHHHHHHHEEEECEEECCCCCC
>Mature Secondary Structure 
SSLRSTMAHAPQIKIPATYMRGGTSKGVFFSLKDLPEAAQIPGPARDALLLRVIGSPDP
CHHHHHHHCCCCEECCHHHHCCCCCCCEEEEECCCCCHHCCCCCCCCEEEEEEECCCCC
YDKQIDGMGGATSSTSKTVILSKSIKADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGS
CCCHHCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHH
FAISNGLVDASRIPHNGVAVVRVWQANIGKTIIAHVPITNGEVQETGDFELDGVTFPAAE
HHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHE
VQVEFMDPAAEEEGGGGSMFPTGNLIDDLEVPGVGTFKATMINAGIPTIFVNAEDIGYTG
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECHHCCCCH
TELQSAINSDPKALQMFETIRAYGALRMGLISSLDEAARRQHTPKVAFVAKPADYVASSG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCC
KAIGAGDVDLLVRALSMGKLHHAMMGTAAVAIGTAAAISGTLVNLAAGGVERNAVRFGHP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCCCEEEECCC
SGTLRVGAEASLENGEWVVKKAIMSRSARVLMEGYVRVPGDSF
CCCEEECCCCCCCCCHHHHHHHHHHHHHHEEEECEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA