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The map label for this gene is prpD [H]

Identifier: 77457995

GI number: 77457995

Start: 1965217

End: 1966701

Strand: Direct

Name: prpD [H]

Synonym: Pfl01_1768

Alternate gene names: 77457995

Gene position: 1965217-1966701 (Clockwise)

Preceding gene: 77457994

Following gene: 77457997

Centisome position: 30.52

GC content: 60.61

Gene sequence:

>1485_bases
ATGAGCGCCAACGTCGACCTGAACAACCGCCCCGACTACGACCATGTCCTGCAGGATATCGCCGATTACGTCCTCAGCTA
CAAAATCACATCCGCCGAAGCCCTCGACACCGCCCGCAACTGCCTGATGGACACCCTGGGCTGCGGCCTGCTGGCCCTGC
GTTTCCCCGAATGCACCAAACACCTTGGCCCAATCGTCGAAGGCACCGTCGTCCCATTCGGCGCCCGAGTCCCTGGCACC
TCCTATCGCCTCGACCCGGTGAAAGCGGCGTGGGACATCGGCTGCATCGTGCGCTGGCTCGATTACAACGACACCTGGCT
CGCCGCTGAATGGGGTCATCCGTCGGACAACCTCGGCGGTATTCTCGCGGTGGCCGATCACCTGTCGCAAAAGCGTCTGG
CCAACGGCGAAGCGCCGCTGACCGTGCGCGATGTACTTGAAGCCATGATCATGGCCCACGAAATCCAGGGCGTGATCGCG
CTGGAGAACTCCTTCAATCGCGTAGGCCTCGACCATGTCATTCTGGTGAAAGTCGCCTCGACCGCCGTGACCGCCAAGCT
GATGGGCGCCAACCGTGAGCAGTTGTTATCGGCGTTGTCTCACGCGTTCGCCGACGGCCAGGCATTGCGCACTTACCGCC
ATGCACCAAACGCCGGCTCGCGTAAATCCTGGGCGGCAGGGGATGCATCGAGCCGGGGCGTGCGGCTGGCGGATATCGCG
ATGCGCGGCGAAATGGGCATTCCCGGCGTCTTGAGTGCCAGACAGTGGGGCTTTTACGACGTTCTGTTCAGCCACACCAA
CAACGATCTGGCGCTCAAACCCGAAGACAAACGCACCTTCAGCTTCTCGCGACCGTTCGGCAGTTACGTGATGGAAAACG
TGCTGTTCAAGATCAGCTTCCCCGCCGAGTTTCACGCGCAAACCGCCTGCGAGGCCGCCGTGACCCTGCATCCGCAGGTA
CGCAATCGTCTGCATGAAATTGATCGGATCGTCATCACTACCCACGAGTCGGCGATCCGCATCATTTCCAAGGTCGGGCC
GTTGGCCAATGCCGCCGACCGCGATCACTGCCTCCAGTACATGACCGCCGTGCCGCTGGCGTTCGGCAATCTGGTAGCGG
AGCAGTACGAGGATGATTTCCACAAGGCCCATCCGATCATCGATGTGCTGCGCGAGAAAATGGTCATCGTCGAAGATCCG
CGCTTCACCCGTGAATATCTGGAGGCCGACAAACGCTCGATTGCCAACGCGGTGCAGGTGTTTTTCAAGGACGGTTCGAG
CACGGAAAACGTGGTGGTGGAATACCCGATCGGCCATCGCCGGCGTCGCGCCGAGGGCATTCCATTGCTGGAGGACAAGT
TCAGGGCAAATCTGGCCACCCGTTTCACCGGGCAGCGCAGCGGGGAGATTCTTGCGCTGTGCAAGGATTCGGCGCGGCTT
GAAGCCACGCCGGTGAATCGGTTTATGGATTTATTGGTTATTTGA

Upstream 100 bases:

>100_bases
CGAGCCCGTTCGCTCTCACCGCCATGCACATTTCAATAAGAAGAAAATTAGCCCTGAACCGAGGTCCCATCAACGAACCA
CTTTCAAGAGTGAATCGAAC

Downstream 100 bases:

>100_bases
AGCGCCGCTCCACGCCTTTTTCCACGAGGATCTTCGCGGAGATTTCTTCAACGGAGAAATGCGTGGAGTTGATGTGCGGA
ATGTTCTCGCGGCGGAACAG

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSANVDLNNRPDYDHVLQDIADYVLSYKITSAEALDTARNCLMDTLGCGLLALRFPECTKHLGPIVEGTVVPFGARVPGT
SYRLDPVKAAWDIGCIVRWLDYNDTWLAAEWGHPSDNLGGILAVADHLSQKRLANGEAPLTVRDVLEAMIMAHEIQGVIA
LENSFNRVGLDHVILVKVASTAVTAKLMGANREQLLSALSHAFADGQALRTYRHAPNAGSRKSWAAGDASSRGVRLADIA
MRGEMGIPGVLSARQWGFYDVLFSHTNNDLALKPEDKRTFSFSRPFGSYVMENVLFKISFPAEFHAQTACEAAVTLHPQV
RNRLHEIDRIVITTHESAIRIISKVGPLANAADRDHCLQYMTAVPLAFGNLVAEQYEDDFHKAHPIIDVLREKMVIVEDP
RFTREYLEADKRSIANAVQVFFKDGSSTENVVVEYPIGHRRRRAEGIPLLEDKFRANLATRFTGQRSGEILALCKDSARL
EATPVNRFMDLLVI

Sequences:

>Translated_494_residues
MSANVDLNNRPDYDHVLQDIADYVLSYKITSAEALDTARNCLMDTLGCGLLALRFPECTKHLGPIVEGTVVPFGARVPGT
SYRLDPVKAAWDIGCIVRWLDYNDTWLAAEWGHPSDNLGGILAVADHLSQKRLANGEAPLTVRDVLEAMIMAHEIQGVIA
LENSFNRVGLDHVILVKVASTAVTAKLMGANREQLLSALSHAFADGQALRTYRHAPNAGSRKSWAAGDASSRGVRLADIA
MRGEMGIPGVLSARQWGFYDVLFSHTNNDLALKPEDKRTFSFSRPFGSYVMENVLFKISFPAEFHAQTACEAAVTLHPQV
RNRLHEIDRIVITTHESAIRIISKVGPLANAADRDHCLQYMTAVPLAFGNLVAEQYEDDFHKAHPIIDVLREKMVIVEDP
RFTREYLEADKRSIANAVQVFFKDGSSTENVVVEYPIGHRRRRAEGIPLLEDKFRANLATRFTGQRSGEILALCKDSARL
EATPVNRFMDLLVI
>Mature_493_residues
SANVDLNNRPDYDHVLQDIADYVLSYKITSAEALDTARNCLMDTLGCGLLALRFPECTKHLGPIVEGTVVPFGARVPGTS
YRLDPVKAAWDIGCIVRWLDYNDTWLAAEWGHPSDNLGGILAVADHLSQKRLANGEAPLTVRDVLEAMIMAHEIQGVIAL
ENSFNRVGLDHVILVKVASTAVTAKLMGANREQLLSALSHAFADGQALRTYRHAPNAGSRKSWAAGDASSRGVRLADIAM
RGEMGIPGVLSARQWGFYDVLFSHTNNDLALKPEDKRTFSFSRPFGSYVMENVLFKISFPAEFHAQTACEAAVTLHPQVR
NRLHEIDRIVITTHESAIRIISKVGPLANAADRDHCLQYMTAVPLAFGNLVAEQYEDDFHKAHPIIDVLREKMVIVEDPR
FTREYLEADKRSIANAVQVFFKDGSSTENVVVEYPIGHRRRRAEGIPLLEDKFRANLATRFTGQRSGEILALCKDSARLE
ATPVNRFMDLLVI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=497, Percent_Identity=60.7645875251509, Blast_Score=612, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6325258, Length=504, Percent_Identity=54.9603174603175, Blast_Score=528, Evalue=1e-151,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 54784; Mature: 54653

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANVDLNNRPDYDHVLQDIADYVLSYKITSAEALDTARNCLMDTLGCGLLALRFPECTK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HLGPIVEGTVVPFGARVPGTSYRLDPVKAAWDIGCIVRWLDYNDTWLAAEWGHPSDNLGG
HCCCCCCCCEECCCCCCCCCCEECCCHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCH
ILAVADHLSQKRLANGEAPLTVRDVLEAMIMAHEIQGVIALENSFNRVGLDHVILVKVAS
HHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEEEHHH
TAVTAKLMGANREQLLSALSHAFADGQALRTYRHAPNAGSRKSWAAGDASSRGVRLADIA
HHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEE
MRGEMGIPGVLSARQWGFYDVLFSHTNNDLALKPEDKRTFSFSRPFGSYVMENVLFKISF
ECCCCCCCCCCCCCCCCCEEEHEECCCCCEEECCCCCCEEEECCCHHHHHHHHHHEEEEC
PAEFHAQTACEAAVTLHPQVRNRLHEIDRIVITTHESAIRIISKVGPLANAADRDHCLQY
CHHHHHHHHHHHHEEECHHHHHHHHHHCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHH
MTAVPLAFGNLVAEQYEDDFHKAHPIIDVLREKMVIVEDPRFTREYLEADKRSIANAVQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHH
FFKDGSSTENVVVEYPIGHRRRRAEGIPLLEDKFRANLATRFTGQRSGEILALCKDSARL
HHCCCCCCCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCE
EATPVNRFMDLLVI
ECCHHHHHHHHHCC
>Mature Secondary Structure 
SANVDLNNRPDYDHVLQDIADYVLSYKITSAEALDTARNCLMDTLGCGLLALRFPECTK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HLGPIVEGTVVPFGARVPGTSYRLDPVKAAWDIGCIVRWLDYNDTWLAAEWGHPSDNLGG
HCCCCCCCCEECCCCCCCCCCEECCCHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCH
ILAVADHLSQKRLANGEAPLTVRDVLEAMIMAHEIQGVIALENSFNRVGLDHVILVKVAS
HHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEEEHHH
TAVTAKLMGANREQLLSALSHAFADGQALRTYRHAPNAGSRKSWAAGDASSRGVRLADIA
HHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEE
MRGEMGIPGVLSARQWGFYDVLFSHTNNDLALKPEDKRTFSFSRPFGSYVMENVLFKISF
ECCCCCCCCCCCCCCCCCEEEHEECCCCCEEECCCCCCEEEECCCHHHHHHHHHHEEEEC
PAEFHAQTACEAAVTLHPQVRNRLHEIDRIVITTHESAIRIISKVGPLANAADRDHCLQY
CHHHHHHHHHHHHEEECHHHHHHHHHHCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHH
MTAVPLAFGNLVAEQYEDDFHKAHPIIDVLREKMVIVEDPRFTREYLEADKRSIANAVQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHH
FFKDGSSTENVVVEYPIGHRRRRAEGIPLLEDKFRANLATRFTGQRSGEILALCKDSARL
HHCCCCCCCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCE
EATPVNRFMDLLVI
ECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]