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The map label for this gene is pucI [H]

Identifier: 77457983

GI number: 77457983

Start: 1949913

End: 1951457

Strand: Reverse

Name: pucI [H]

Synonym: Pfl01_1756

Alternate gene names: 77457983

Gene position: 1951457-1949913 (Counterclockwise)

Preceding gene: 77457984

Following gene: 77457982

Centisome position: 30.31

GC content: 61.36

Gene sequence:

>1545_bases
ATGCGTACGAGTCTCTCCAATAACAACATCGCGCTGAATCTGCCCGCCTCTTCACCCCTCGACCACAGCGTGCCCGGTGA
TGGCATCGCCGAACCACTGCAACTGAGCCCGCGTCTGCACAACAGCGACCTCGCGCCGACCAAGGTCGAAGGACGGCGCT
GGGGCAAATACAGCATCTTTGCCCTGTGGACCAACGATGTGCACAACATCGCCAACTACTCGTTTGCCATCGGTCTGTAC
GCCTTGGGCCTGGGCGGCTGGCAGATTCTGCTGTCGCTGGGGATCGGCGCGGCGCTGGTGTACTTCTTCATGAACCTGTC
CGGCTACATGGGGCAGAAAACCGGGGTGCCGTTTCCGGTGATCAGCCGGATCAGTTTCGGCATTCACGGGGCACAGATTC
CTGCATTGATCCGGGCGGTGATCGCCATCGCCTGGTTCGGGATTCAGACGTACCTGGCGTCGGTGGTGTTTCGCGTGTTG
CTCACGGCAATCCATCCCGGTTTCGCCGACTACGACCACAACTCGATCCTCGGCCTGTCGAGCCTGGGCTGGGTGTGTTT
CGTGGCGATCTGGTTTGTGCAGTTGGGGATTCTGGCGTATGGCATGGAAATGGTCCGCCGCTACGAAGCCTTCGCCGGGC
CGGTGATTCTGCTGACCGTCGCCTGCCTCGCCGCGTGGATGTACACCCAGGCCAACGCTACCATTGCCTGGTCGATTCGC
GAGCCGCTGACCGGCGCCGAGATGTGGCGCAACATCTTTGCCGGCGGCGCCTTGTGGCTGGCGATCTACGGCACGCTGAT
CCTCAACTTTTGCGATTTCGCTCGCTCTTCACCGTGCCGCAAAACCATCAAGGTCGGAAATTTCTGGGGCCTGCCGGTCA
ATATTCTGGTGTTCGCCGGGATCACCGTCCTGCTGTGCGGTGCGCAATTTCACATCAACGGGCGGATCATCGAAAGCCCG
ACCGAAATCATTGCGTCGATCCCCAACACGTTCTTTCTGGTACTCGGATGCCTGGCGTTCCTGATCGTCACCGTGGCGGT
GAACATTATGGCCAACTTCGTCGCCCCGGCCTTCGTGCTGAGCAACCTGGCGCCTAAATACCTGACCTTCCGCCGCGCCG
GGCTGATCAGCGCAACGATTGCCGTACTGATCCTGCCGTGGAACCTCTACAACAGCCCGCTGGTGATCGTGTACTTCCTG
TCCGGCCTCGGCGCCCTGCTCGGCCCGTTGTACGGCGTGATCATGGTCGACTACTGGCTGATCCGCAAAGGCCGGATCCA
CGTGCCGCAGCTGTACAGCGAACACCCGAACGGTGCGTATTACTACAGTCGCGGGGTCAATCTGCGGGCGGTCGCAGCGT
TTATTCCGGCCGCCTTGATCGCGATCGTCCTGGCGCTGGTGCCGGGCTTCCACAGCGTTTCGCCCTTCTCCTGGCTGATC
GGCGCCGGCATTGCCGGGATGCTCTACCTGATCATCGCCAAACGCCAGCCGCACTACGCCGACGTCGACGGTGAAGCCAT
CGCCGTCGACAACGTCAGCCACTGA

Upstream 100 bases:

>100_bases
GCCCCGTAACCAGTGATGTTTTCACCGCCGCGACGAAGATCTGCCGAGCCCGAACGCTCATGCACAGTCATCGCGGCCCG
AACATCAGGAGCCTGCCGGA

Downstream 100 bases:

>100_bases
ATTTTTTGGCGGCCCTTTCCGGAGCCGCAGAACCAACCGCAATAAGGACTTCCCATGCGCATTCTCGTGGTCAACGTCAA
CACCACCGAATCCATCACCC

Product: cytosine/purines, uracil, thiamine, allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MRTSLSNNNIALNLPASSPLDHSVPGDGIAEPLQLSPRLHNSDLAPTKVEGRRWGKYSIFALWTNDVHNIANYSFAIGLY
ALGLGGWQILLSLGIGAALVYFFMNLSGYMGQKTGVPFPVISRISFGIHGAQIPALIRAVIAIAWFGIQTYLASVVFRVL
LTAIHPGFADYDHNSILGLSSLGWVCFVAIWFVQLGILAYGMEMVRRYEAFAGPVILLTVACLAAWMYTQANATIAWSIR
EPLTGAEMWRNIFAGGALWLAIYGTLILNFCDFARSSPCRKTIKVGNFWGLPVNILVFAGITVLLCGAQFHINGRIIESP
TEIIASIPNTFFLVLGCLAFLIVTVAVNIMANFVAPAFVLSNLAPKYLTFRRAGLISATIAVLILPWNLYNSPLVIVYFL
SGLGALLGPLYGVIMVDYWLIRKGRIHVPQLYSEHPNGAYYYSRGVNLRAVAAFIPAALIAIVLALVPGFHSVSPFSWLI
GAGIAGMLYLIIAKRQPHYADVDGEAIAVDNVSH

Sequences:

>Translated_514_residues
MRTSLSNNNIALNLPASSPLDHSVPGDGIAEPLQLSPRLHNSDLAPTKVEGRRWGKYSIFALWTNDVHNIANYSFAIGLY
ALGLGGWQILLSLGIGAALVYFFMNLSGYMGQKTGVPFPVISRISFGIHGAQIPALIRAVIAIAWFGIQTYLASVVFRVL
LTAIHPGFADYDHNSILGLSSLGWVCFVAIWFVQLGILAYGMEMVRRYEAFAGPVILLTVACLAAWMYTQANATIAWSIR
EPLTGAEMWRNIFAGGALWLAIYGTLILNFCDFARSSPCRKTIKVGNFWGLPVNILVFAGITVLLCGAQFHINGRIIESP
TEIIASIPNTFFLVLGCLAFLIVTVAVNIMANFVAPAFVLSNLAPKYLTFRRAGLISATIAVLILPWNLYNSPLVIVYFL
SGLGALLGPLYGVIMVDYWLIRKGRIHVPQLYSEHPNGAYYYSRGVNLRAVAAFIPAALIAIVLALVPGFHSVSPFSWLI
GAGIAGMLYLIIAKRQPHYADVDGEAIAVDNVSH
>Mature_514_residues
MRTSLSNNNIALNLPASSPLDHSVPGDGIAEPLQLSPRLHNSDLAPTKVEGRRWGKYSIFALWTNDVHNIANYSFAIGLY
ALGLGGWQILLSLGIGAALVYFFMNLSGYMGQKTGVPFPVISRISFGIHGAQIPALIRAVIAIAWFGIQTYLASVVFRVL
LTAIHPGFADYDHNSILGLSSLGWVCFVAIWFVQLGILAYGMEMVRRYEAFAGPVILLTVACLAAWMYTQANATIAWSIR
EPLTGAEMWRNIFAGGALWLAIYGTLILNFCDFARSSPCRKTIKVGNFWGLPVNILVFAGITVLLCGAQFHINGRIIESP
TEIIASIPNTFFLVLGCLAFLIVTVAVNIMANFVAPAFVLSNLAPKYLTFRRAGLISATIAVLILPWNLYNSPLVIVYFL
SGLGALLGPLYGVIMVDYWLIRKGRIHVPQLYSEHPNGAYYYSRGVNLRAVAAFIPAALIAIVLALVPGFHSVSPFSWLI
GAGIAGMLYLIIAKRQPHYADVDGEAIAVDNVSH

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=486, Percent_Identity=30.4526748971193, Blast_Score=195, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6319495, Length=469, Percent_Identity=26.0127931769723, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6319429, Length=517, Percent_Identity=25.531914893617, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6322219, Length=439, Percent_Identity=25.7403189066059, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324645, Length=434, Percent_Identity=23.2718894009217, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324766, Length=440, Percent_Identity=23.6363636363636, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323266, Length=118, Percent_Identity=31.3559322033898, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 55949; Mature: 55949

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSLSNNNIALNLPASSPLDHSVPGDGIAEPLQLSPRLHNSDLAPTKVEGRRWGKYSIF
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEE
ALWTNDVHNIANYSFAIGLYALGLGGWQILLSLGIGAALVYFFMNLSGYMGQKTGVPFPV
EEECCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ISRISFGIHGAQIPALIRAVIAIAWFGIQTYLASVVFRVLLTAIHPGFADYDHNSILGLS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHH
SLGWVCFVAIWFVQLGILAYGMEMVRRYEAFAGPVILLTVACLAAWMYTQANATIAWSIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEECC
EPLTGAEMWRNIFAGGALWLAIYGTLILNFCDFARSSPCRKTIKVGNFWGLPVNILVFAG
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHH
ITVLLCGAQFHINGRIIESPTEIIASIPNTFFLVLGCLAFLIVTVAVNIMANFVAPAFVL
HHHHHCCCEEEECCEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNLAPKYLTFRRAGLISATIAVLILPWNLYNSPLVIVYFLSGLGALLGPLYGVIMVDYWL
HHCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRKGRIHVPQLYSEHPNGAYYYSRGVNLRAVAAFIPAALIAIVLALVPGFHSVSPFSWLI
HHCCCCCCCHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GAGIAGMLYLIIAKRQPHYADVDGEAIAVDNVSH
HHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MRTSLSNNNIALNLPASSPLDHSVPGDGIAEPLQLSPRLHNSDLAPTKVEGRRWGKYSIF
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEE
ALWTNDVHNIANYSFAIGLYALGLGGWQILLSLGIGAALVYFFMNLSGYMGQKTGVPFPV
EEECCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ISRISFGIHGAQIPALIRAVIAIAWFGIQTYLASVVFRVLLTAIHPGFADYDHNSILGLS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHH
SLGWVCFVAIWFVQLGILAYGMEMVRRYEAFAGPVILLTVACLAAWMYTQANATIAWSIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEECC
EPLTGAEMWRNIFAGGALWLAIYGTLILNFCDFARSSPCRKTIKVGNFWGLPVNILVFAG
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHH
ITVLLCGAQFHINGRIIESPTEIIASIPNTFFLVLGCLAFLIVTVAVNIMANFVAPAFVL
HHHHHCCCEEEECCEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNLAPKYLTFRRAGLISATIAVLILPWNLYNSPLVIVYFLSGLGALLGPLYGVIMVDYWL
HHCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRKGRIHVPQLYSEHPNGAYYYSRGVNLRAVAAFIPAALIAIVLALVPGFHSVSPFSWLI
HHCCCCCCCHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GAGIAGMLYLIIAKRQPHYADVDGEAIAVDNVSH
HHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]