Click here to switch to the map view.

The map label for this gene is fadB [C]

Identifier: 77457971

GI number: 77457971

Start: 1937177

End: 1937866

Strand: Reverse

Name: fadB [C]

Synonym: Pfl01_1744

Alternate gene names: 77457971

Gene position: 1937866-1937177 (Counterclockwise)

Preceding gene: 77457973

Following gene: 77457970

Centisome position: 30.1

GC content: 61.45

Gene sequence:

>690_bases
ATGAGCGACCTGATTGCCTACCACCTCGAAGACGGTATCGCGACCCTGACCCTGAGCAACGGCAAGGTCAACGCCATTTC
CCCGGACGTGATCGCGGCCTTCAACGCGGCGCTGGATCAGGCGGTGGCGGATCGTGCCATTGTGATCATCACCGGTCAGC
CAGGCATTCTCTCTGGCGGCTATGACCTGAAAGTGATGACTGCTGGCCCTAAAGAGGCCGTGTCGCTGGTGACCGCCGGC
TCGACCCTGGCCCGTCGCCTGTTGTCGCACCCGTTCCCGGTAATCGTTGCTTGCCCGGGGCATGCCGTGGCCAAAGGCGC
GTTCATTCTGTTGTCCGCCGACTATCGGATCGGTGTCGACGGCCCGTTCAGCATTGGTCTGAACGAAGTGCAGATCGGCA
TGACCATGCACCACGCCGGCATCGAGCTGGCCCGTGATCGCCTGCGTCGCTCGGCCTTCCACCGCTCGGTGATCAACGGC
GAGATGTTCGATCCGCAAAGTGCAGTGGATGCCGGCTTCCTCGACAAAGTGGTCGCAGCCGAAGAACTGCAAGGCGCTGC
CCTCGCCGCCGCGCGTCAGTTGAAGAAGATCAACATGACCGCGCACAAGAACACCAAGCTGAAAGTGCGCAAGGCGCTGC
TGGAAACCCTGGACAACGCAATCATCCAGGATCAGGAACACTTGGGCTGA

Upstream 100 bases:

>100_bases
GATAAGCCGCTCAAGGGTGACCGGTGGGTCAGTCACAGTGCTTTACCTGACCGGCGACGGTGGGTAACGTCTGCCCATCT
TTCTTACAAGGAATTGCGCC

Downstream 100 bases:

>100_bases
CACCCGGCTCATACCGATTCAGAAAAGCCCGACCGTCGTGTCGGGCTTTTTCTTGCACGCCCCGTTGAAAACTCCGCAGC
AGCTCTAGGACGCCTCTGAC

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Enoyl-CoA Hydratase/Isomerase; Enoyl-CoA Hydratase/Isomerase Family Protein; Enoyl-CoA Hydratase/Carnithine Racemase

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSDLIAYHLEDGIATLTLSNGKVNAISPDVIAAFNAALDQAVADRAIVIITGQPGILSGGYDLKVMTAGPKEAVSLVTAG
STLARRLLSHPFPVIVACPGHAVAKGAFILLSADYRIGVDGPFSIGLNEVQIGMTMHHAGIELARDRLRRSAFHRSVING
EMFDPQSAVDAGFLDKVVAAEELQGAALAAARQLKKINMTAHKNTKLKVRKALLETLDNAIIQDQEHLG

Sequences:

>Translated_229_residues
MSDLIAYHLEDGIATLTLSNGKVNAISPDVIAAFNAALDQAVADRAIVIITGQPGILSGGYDLKVMTAGPKEAVSLVTAG
STLARRLLSHPFPVIVACPGHAVAKGAFILLSADYRIGVDGPFSIGLNEVQIGMTMHHAGIELARDRLRRSAFHRSVING
EMFDPQSAVDAGFLDKVVAAEELQGAALAAARQLKKINMTAHKNTKLKVRKALLETLDNAIIQDQEHLG
>Mature_228_residues
SDLIAYHLEDGIATLTLSNGKVNAISPDVIAAFNAALDQAVADRAIVIITGQPGILSGGYDLKVMTAGPKEAVSLVTAGS
TLARRLLSHPFPVIVACPGHAVAKGAFILLSADYRIGVDGPFSIGLNEVQIGMTMHHAGIELARDRLRRSAFHRSVINGE
MFDPQSAVDAGFLDKVVAAEELQGAALAAARQLKKINMTAHKNTKLKVRKALLETLDNAIIQDQEHLG

Specific function: FADb And FADa Are The Alpha And Beta Subunits Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI62530384, Length=206, Percent_Identity=30.0970873786408, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI295842266, Length=205, Percent_Identity=28.780487804878, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI31542718, Length=215, Percent_Identity=28.3720930232558, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24583165, Length=221, Percent_Identity=26.6968325791855, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.17

Molecular weight: Translated: 24249; Mature: 24118

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLIAYHLEDGIATLTLSNGKVNAISPDVIAAFNAALDQAVADRAIVIITGQPGILSGG
CCCCEEEEECCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
YDLKVMTAGPKEAVSLVTAGSTLARRLLSHPFPVIVACPGHAVAKGAFILLSADYRIGVD
CEEEEEECCCHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCHHHCCEEEEEECCEEECCC
GPFSIGLNEVQIGMTMHHAGIELARDRLRRSAFHRSVINGEMFDPQSAVDAGFLDKVVAA
CCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
EELQGAALAAARQLKKINMTAHKNTKLKVRKALLETLDNAIIQDQEHLG
HHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
SDLIAYHLEDGIATLTLSNGKVNAISPDVIAAFNAALDQAVADRAIVIITGQPGILSGG
CCCEEEEECCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
YDLKVMTAGPKEAVSLVTAGSTLARRLLSHPFPVIVACPGHAVAKGAFILLSADYRIGVD
CEEEEEECCCHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCHHHCCEEEEEECCEEECCC
GPFSIGLNEVQIGMTMHHAGIELARDRLRRSAFHRSVINGEMFDPQSAVDAGFLDKVVAA
CCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
EELQGAALAAARQLKKINMTAHKNTKLKVRKALLETLDNAIIQDQEHLG
HHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.066 {acetoacetyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA