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The map label for this gene is cobS

Identifier: 77457877

GI number: 77457877

Start: 1840487

End: 1841218

Strand: Direct

Name: cobS

Synonym: Pfl01_1650

Alternate gene names: 77457877

Gene position: 1840487-1841218 (Clockwise)

Preceding gene: 77457876

Following gene: 77457878

Centisome position: 28.59

GC content: 62.84

Gene sequence:

>732_bases
ATGCTGCCTCTGTGGATCGCCCTGCAATTTCTCGGCAGCCTGCCGATTCGTCTGCCGGGGATGCCGACGCCCGAACAGCT
TGGCCGGTCGCTGCTGTTTTATCCGCTGGTGGGCCTGTTGTTCGGTGTCATTCTCTGGGCGCTGAACATTGCCTTGGCCG
GCACGCCGTTGCTGTTGCATGCGGCGCTGTTGCTGGCGGTGTGGGTGCTGCTCAGCGGCGCGTTGCACCTTGATGGCCTG
GCGGACAGCGCTGACGCGTGGCTCGGCGGTTTCGGTGATCGTGAGCGTACGCTGACGATCATGAAGGATCCGCGCAGCGG
GCCGATCGCCGTAGTGACGCTGGTGCTGGTGTTGCTGCTCAAGTTCGCCGCGTTGCTGGCGTTGATCGAACAACAGCAAA
GCATGGCGTTGATCATCGTGCCGCTGATCGGGCGAGCGGCGCTGCTCGGGCTGTTTCTCACCACGTCTTATGTGCGGGCT
GGCGGGTTAGGGCAGGCGCTGGCCGATCATCTGCCACGCAAGACAGGTTGGCAGGTGCTGGCGGTGAGTGCGGCGGTGTG
TTTGGTGATTGCCGGGTTCAATGCTGTCGTGGCGTTGCTGCTGGCGGTCATCGTGTTCATCTGGCTGCGGCATTTGATGG
TGCGACGACTGGGTGGGACCACCGGTGATACGGCCGGTGCGTTGCTGGAATTGCTGGAGATGTCAGTGCTGGTCGGGCTG
GCCTTGTTTTGA

Upstream 100 bases:

>100_bases
GGTTTGCCTCGCGAACAACTGCTCAATGTCGAAGTCGGTCATGGTGCGCTGTTTTCGCTGATCGTCGAAGCCGACGGTTC
GCTCAGGGAAGTGCTCTGAC

Downstream 100 bases:

>100_bases
TAAGTAACTTTGATCTGTAACTTGATTTAACACAGTCGCGGGTATATACACGCATCATGCTTCCATCTCAGTGTTTGTGC
ACCAACCTGCGACGCGCCGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLPLWIALQFLGSLPIRLPGMPTPEQLGRSLLFYPLVGLLFGVILWALNIALAGTPLLLHAALLLAVWVLLSGALHLDGL
ADSADAWLGGFGDRERTLTIMKDPRSGPIAVVTLVLVLLLKFAALLALIEQQQSMALIIVPLIGRAALLGLFLTTSYVRA
GGLGQALADHLPRKTGWQVLAVSAAVCLVIAGFNAVVALLLAVIVFIWLRHLMVRRLGGTTGDTAGALLELLEMSVLVGL
ALF

Sequences:

>Translated_243_residues
MLPLWIALQFLGSLPIRLPGMPTPEQLGRSLLFYPLVGLLFGVILWALNIALAGTPLLLHAALLLAVWVLLSGALHLDGL
ADSADAWLGGFGDRERTLTIMKDPRSGPIAVVTLVLVLLLKFAALLALIEQQQSMALIIVPLIGRAALLGLFLTTSYVRA
GGLGQALADHLPRKTGWQVLAVSAAVCLVIAGFNAVVALLLAVIVFIWLRHLMVRRLGGTTGDTAGALLELLEMSVLVGL
ALF
>Mature_243_residues
MLPLWIALQFLGSLPIRLPGMPTPEQLGRSLLFYPLVGLLFGVILWALNIALAGTPLLLHAALLLAVWVLLSGALHLDGL
ADSADAWLGGFGDRERTLTIMKDPRSGPIAVVTLVLVLLLKFAALLALIEQQQSMALIIVPLIGRAALLGLFLTTSYVRA
GGLGQALADHLPRKTGWQVLAVSAAVCLVIAGFNAVVALLLAVIVFIWLRHLMVRRLGGTTGDTAGALLELLEMSVLVGL
ALF

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=32.520325203252, Blast_Score=88, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_PSEPF (Q3KFR3)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_347382.1
- STRING:   Q3KFR3
- GeneID:   3716784
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_1650
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   ELTECAV
- ProtClustDB:   PRK00235
- BioCyc:   PFLU205922:PFL_1650-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 25784; Mature: 25784

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xeefcf70)-; HASH(0xb20d420)-; HASH(0xf3a4f8c)-; HASH(0xedb47bc)-; HASH(0xf83565c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLWIALQFLGSLPIRLPGMPTPEQLGRSLLFYPLVGLLFGVILWALNIALAGTPLLLH
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AALLLAVWVLLSGALHLDGLADSADAWLGGFGDRERTLTIMKDPRSGPIAVVTLVLVLLL
HHHHHHHHHHHHCCHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
KFAALLALIEQQQSMALIIVPLIGRAALLGLFLTTSYVRAGGLGQALADHLPRKTGWQVL
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHH
AVSAAVCLVIAGFNAVVALLLAVIVFIWLRHLMVRRLGGTTGDTAGALLELLEMSVLVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALF
HCH
>Mature Secondary Structure
MLPLWIALQFLGSLPIRLPGMPTPEQLGRSLLFYPLVGLLFGVILWALNIALAGTPLLLH
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AALLLAVWVLLSGALHLDGLADSADAWLGGFGDRERTLTIMKDPRSGPIAVVTLVLVLLL
HHHHHHHHHHHHCCHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
KFAALLALIEQQQSMALIIVPLIGRAALLGLFLTTSYVRAGGLGQALADHLPRKTGWQVL
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHH
AVSAAVCLVIAGFNAVVALLLAVIVFIWLRHLMVRRLGGTTGDTAGALLELLEMSVLVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALF
HCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA