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The map label for this gene is hda [H]

Identifier: 77457863

GI number: 77457863

Start: 1827637

End: 1828341

Strand: Direct

Name: hda [H]

Synonym: Pfl01_1636

Alternate gene names: 77457863

Gene position: 1827637-1828341 (Clockwise)

Preceding gene: 77457862

Following gene: 77457864

Centisome position: 28.39

GC content: 58.58

Gene sequence:

>705_bases
ATGAAACCGATTCAGCTGCCCCTAGGTGTGCGTCTGCGTGACGACGCCACCTTCATCAACTACTACCCAGGCGCCAATGC
CGCTGCACTCGGCTATGTCGAGCGTCTATGCGAAGCCGACGCCGGCTGGACCGAAAGCCTGATCTATCTGTGGGGCAAGC
ACGGAGTAGGGCGTACGCATCTGTTGCAGGCCGCGTGCCTGCGCTTCGAACAGATGGGTGAACCGGCGGTGTACCTGCCG
CTGGCCGAGTTGATGGATCGCGGCATCGAAATTCTCGACAACCTCGAACAGTACGAACTGGTCTGTCTGGACGACTTGCA
GGTGATTGCCGGCAAGGCGGACTGGGAAGAGGCAATGTTTCATCTGTTCAACCGTCTGCGTGACAGCGGTCGGCGTCTGC
TGATTGCGGCGTCCACTTCACCCCGTGAACTGCCGATCAAACTGGCTGACCTGAAATCACGGCTGACCCTGGCGCTGATC
TTCCAGATGCGTCCGCTCTCCGACGAAGACAAATTGCGTGCCCTGCAACTGCGTGCTTCACGTCGCGGTCTGCACCTGAC
TGACGAGGTTGGGCATTTTATTTTGACTCGCGGTACCCGCAGTATGAGCGCGCTTTTCGATCTGCTGGAGCAGCTCGATC
AGGCCTCTTTGCAGGCTCAGCGCAAGCTGACCATTCCCTTCCTGAAAGAAACCCTGGGCTGGTAA

Upstream 100 bases:

>100_bases
GCTTGGGGTCTGTCGCATTGTGCGCTCCGCTTCACGGGTATAAACTTCGCTCACTTTACACAGAGGCCACTAACGGTTCC
TTGGGAACTGTTCAGTCAGC

Downstream 100 bases:

>100_bases
AACCGGGGCTTTCAGCCGGTTTTGGCATATTTCAGGCGCCAGAAAACCCGCTTTCCTGTGGGTTGCACAGGCCGCGTCTC
CATTCAAATAGGCTTAAGCG

Product: DNA replication initiation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKPIQLPLGVRLRDDATFINYYPGANAAALGYVERLCEADAGWTESLIYLWGKHGVGRTHLLQAACLRFEQMGEPAVYLP
LAELMDRGIEILDNLEQYELVCLDDLQVIAGKADWEEAMFHLFNRLRDSGRRLLIAASTSPRELPIKLADLKSRLTLALI
FQMRPLSDEDKLRALQLRASRRGLHLTDEVGHFILTRGTRSMSALFDLLEQLDQASLQAQRKLTIPFLKETLGW

Sequences:

>Translated_234_residues
MKPIQLPLGVRLRDDATFINYYPGANAAALGYVERLCEADAGWTESLIYLWGKHGVGRTHLLQAACLRFEQMGEPAVYLP
LAELMDRGIEILDNLEQYELVCLDDLQVIAGKADWEEAMFHLFNRLRDSGRRLLIAASTSPRELPIKLADLKSRLTLALI
FQMRPLSDEDKLRALQLRASRRGLHLTDEVGHFILTRGTRSMSALFDLLEQLDQASLQAQRKLTIPFLKETLGW
>Mature_234_residues
MKPIQLPLGVRLRDDATFINYYPGANAAALGYVERLCEADAGWTESLIYLWGKHGVGRTHLLQAACLRFEQMGEPAVYLP
LAELMDRGIEILDNLEQYELVCLDDLQVIAGKADWEEAMFHLFNRLRDSGRRLLIAASTSPRELPIKLADLKSRLTLALI
FQMRPLSDEDKLRALQLRASRRGLHLTDEVGHFILTRGTRSMSALFDLLEQLDQASLQAQRKLTIPFLKETLGW

Specific function: Mediates the interactions of dnaA with the beta subunit sliding clamp, promoting hydrolysis of dnaA-bound ATP. It is essential for the control of initiation of DNA replication by inhibiting reinitiation of replication [H]

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family. HdA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510964, Length=231, Percent_Identity=47.1861471861472, Blast_Score=203, Evalue=9e-54,
Organism=Escherichia coli, GI2367267, Length=227, Percent_Identity=24.6696035242291, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020591
- InterPro:   IPR017788
- InterPro:   IPR013317
- InterPro:   IPR022864 [H]

Pfam domain/function: PF00308 Bac_DnaA [H]

EC number: NA

Molecular weight: Translated: 26560; Mature: 26560

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIQLPLGVRLRDDATFINYYPGANAAALGYVERLCEADAGWTESLIYLWGKHGVGRTH
CCCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHEECCCCCCHHH
LLQAACLRFEQMGEPAVYLPLAELMDRGIEILDNLEQYELVCLDDLQVIAGKADWEEAMF
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCHHHHEEEEECCHHHHCCCCCHHHHHH
HLFNRLRDSGRRLLIAASTSPRELPIKLADLKSRLTLALIFQMRPLSDEDKLRALQLRAS
HHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
RRGLHLTDEVGHFILTRGTRSMSALFDLLEQLDQASLQAQRKLTIPFLKETLGW
CCCCEEHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MKPIQLPLGVRLRDDATFINYYPGANAAALGYVERLCEADAGWTESLIYLWGKHGVGRTH
CCCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHEECCCCCCHHH
LLQAACLRFEQMGEPAVYLPLAELMDRGIEILDNLEQYELVCLDDLQVIAGKADWEEAMF
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCHHHHEEEEECCHHHHCCCCCHHHHHH
HLFNRLRDSGRRLLIAASTSPRELPIKLADLKSRLTLALIFQMRPLSDEDKLRALQLRAS
HHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
RRGLHLTDEVGHFILTRGTRSMSALFDLLEQLDQASLQAQRKLTIPFLKETLGW
CCCCEEHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA