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The map label for this gene is braZ [H]

Identifier: 77457846

GI number: 77457846

Start: 1811257

End: 1812570

Strand: Direct

Name: braZ [H]

Synonym: Pfl01_1619

Alternate gene names: 77457846

Gene position: 1811257-1812570 (Clockwise)

Preceding gene: 77457845

Following gene: 77457847

Centisome position: 28.13

GC content: 63.62

Gene sequence:

>1314_bases
ATGAAAGTTTTGAAAGGTCAGGACATCCTGGCACTTGGCTTTATGACATTCGCCCTGTTCGTCGGGGCCGGCAACATCAT
CTTCCCGCCTATCGTCGGTTTGCAGTCCGGGCCTAACGTCTGGATCGCGGCGCTGGGCTTCCTGATCACGGCAGTCGGTC
TGCCGGTGGTCACCGTGGTTGCACTGGCCAAGGTCGGTGGCGCAATGGACGCCCTGAGCAGCCCGATCGGCAAGGTCGCC
GGTGGCGTACTGGCTGCCGCGTGCTACCTGGCAGTCGGCCCGCTGTTCGCCACCCCGCGTACCGCGACCGTTTCCTTTGA
AGTGGGTTTGGCGCCGCTGACCGGCGAGAGCCCGCTGGCACTGTTCCTCTACAGTTCGGTGTACTTCCTGCTGGTGTTCT
TCATTTCGCTCTACCCTGGGCGTCTGCTGGACACCGTCGGTCGCTTCCTCGCACCCCTGAAGATCATTGCTCTGGCCGTG
CTCGGCATCGCCGCATTCGCACTGCCGGCCGGTGACATCGGCGTGGCCACTCCGGAATACGTTGCGGCACCGTTCTCCCA
AGGTTTCATCAATGGTTACCTGACCATGGATACCCTCGGCGCGCTGGTGTTCGGCATCGTCATCGTCAACGCGATCCGCT
CCCGTGGCGTCGAGTCGCCGGCGCTGATCACCCGGTACGCGATCATCGCCGGGCTGATTGCCGGCGTCGGTCTGGCGCTG
GTGTATGTCAGCCTGTTCCGTCTGGGTTCCGGCAGCCATGAAGTGGCCGCTGGCGCTACCAATGGCGCGGCGGTGCTGCA
CGCTTACGTGCAACACACCTTCGGTTCGCTGGGTAGCGGTTTCCTTGCGGTGCTGATCTCGCTGGCCTGCCTGGTGACGG
CGGTCGGTCTGACCTGCGCCTGTGCTGAGTACTTCAGCCGCGTGTTGCCACTGTCCTACAAGACACTGGTGATCATCCTG
GCTGCATTCTCGCTGCTGGTATCCAACCTGGGCCTGACCAAGCTGATCGCGTTCTCGATCCCGGTGCTCACCGCGATCTA
CCCGCCGTGCATCGTGCTGGTGGCCCTGAGCTTCTGCGCCGCGTTCTGGCATGAGCAGAGCCGCATCATGGGCCCGGTGA
TGCTGGTGTCGTTCGTGTTCGGCACCATCGATGCGCTGAAAGGCGCCGGTCTGGCGGACTGGATGCCATCGCAACTGGCC
CACCTGCCGCTCAGCGAGCAAGGTCTGGCGTGGCTGGTGCCGTGCGTGATGACCCTGGTGGTTGCCGTGGTTTGCGACCG
CCTGCTGGGCAAGCGCGCCGAAGCACTCGCTTAA

Upstream 100 bases:

>100_bases
CGCGCTAAACGGGTCAGTCTTATACGGATTGACAGCATTTCCCTAACCCCACAGGGAAATCCCCCTCTAAATTCCGATTC
AGTAGTGTGGTATTTCCTTA

Downstream 100 bases:

>100_bases
GATTGCCGGGTCGCCACAAGCGGCTGCCGAGCGATTAAACAGAAATGCCCCGTATCAATCGATACGGGGCATTTTTTATG
GGCTTCAGGCAGTGTCTTTT

Product: branched chain amino acid ABC transporter carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-III [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQSGPNVWIAALGFLITAVGLPVVTVVALAKVGGAMDALSSPIGKVA
GGVLAAACYLAVGPLFATPRTATVSFEVGLAPLTGESPLALFLYSSVYFLLVFFISLYPGRLLDTVGRFLAPLKIIALAV
LGIAAFALPAGDIGVATPEYVAAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPALITRYAIIAGLIAGVGLAL
VYVSLFRLGSGSHEVAAGATNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCACAEYFSRVLPLSYKTLVIIL
AAFSLLVSNLGLTKLIAFSIPVLTAIYPPCIVLVALSFCAAFWHEQSRIMGPVMLVSFVFGTIDALKGAGLADWMPSQLA
HLPLSEQGLAWLVPCVMTLVVAVVCDRLLGKRAEALA

Sequences:

>Translated_437_residues
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQSGPNVWIAALGFLITAVGLPVVTVVALAKVGGAMDALSSPIGKVA
GGVLAAACYLAVGPLFATPRTATVSFEVGLAPLTGESPLALFLYSSVYFLLVFFISLYPGRLLDTVGRFLAPLKIIALAV
LGIAAFALPAGDIGVATPEYVAAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPALITRYAIIAGLIAGVGLAL
VYVSLFRLGSGSHEVAAGATNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCACAEYFSRVLPLSYKTLVIIL
AAFSLLVSNLGLTKLIAFSIPVLTAIYPPCIVLVALSFCAAFWHEQSRIMGPVMLVSFVFGTIDALKGAGLADWMPSQLA
HLPLSEQGLAWLVPCVMTLVVAVVCDRLLGKRAEALA
>Mature_437_residues
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQSGPNVWIAALGFLITAVGLPVVTVVALAKVGGAMDALSSPIGKVA
GGVLAAACYLAVGPLFATPRTATVSFEVGLAPLTGESPLALFLYSSVYFLLVFFISLYPGRLLDTVGRFLAPLKIIALAV
LGIAAFALPAGDIGVATPEYVAAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPALITRYAIIAGLIAGVGLAL
VYVSLFRLGSGSHEVAAGATNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCACAEYFSRVLPLSYKTLVIIL
AAFSLLVSNLGLTKLIAFSIPVLTAIYPPCIVLVALSFCAAFWHEQSRIMGPVMLVSFVFGTIDALKGAGLADWMPSQLA
HLPLSEQGLAWLVPCVMTLVVAVVCDRLLGKRAEALA

Specific function: Component of the LIV-III transport system for branched- chain amino acids. BraZ is specific for isoleucine and valine. The LIV-III transport system may be H(+)-coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=434, Percent_Identity=66.3594470046083, Blast_Score=575, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45175; Mature: 45175

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQSGPNVWIAALGFLITAVGLPVVTVV
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALAKVGGAMDALSSPIGKVAGGVLAAACYLAVGPLFATPRTATVSFEVGLAPLTGESPLA
HHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHH
LFLYSSVYFLLVFFISLYPGRLLDTVGRFLAPLKIIALAVLGIAAFALPAGDIGVATPEY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VAAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPALITRYAIIAGLIAGVGLAL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VYVSLFRLGSGSHEVAAGATNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
CAEYFSRVLPLSYKTLVIILAAFSLLVSNLGLTKLIAFSIPVLTAIYPPCIVLVALSFCA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFWHEQSRIMGPVMLVSFVFGTIDALKGAGLADWMPSQLAHLPLSEQGLAWLVPCVMTLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHCCCCCCCCHHHHHHHHHHHH
VAVVCDRLLGKRAEALA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQSGPNVWIAALGFLITAVGLPVVTVV
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALAKVGGAMDALSSPIGKVAGGVLAAACYLAVGPLFATPRTATVSFEVGLAPLTGESPLA
HHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHH
LFLYSSVYFLLVFFISLYPGRLLDTVGRFLAPLKIIALAVLGIAAFALPAGDIGVATPEY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VAAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPALITRYAIIAGLIAGVGLAL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VYVSLFRLGSGSHEVAAGATNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
CAEYFSRVLPLSYKTLVIILAAFSLLVSNLGLTKLIAFSIPVLTAIYPPCIVLVALSFCA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFWHEQSRIMGPVMLVSFVFGTIDALKGAGLADWMPSQLAHLPLSEQGLAWLVPCVMTLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHCCCCCCCCHHHHHHHHHHHH
VAVVCDRLLGKRAEALA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1900503; 10984043 [H]